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Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989408
Funder
Australian Research Council
Funding Amount
$150,000.00
Summary
A multiple-ion membrane inlet mass spectrometer. There is a compelling need for a modern isotope-ratio mass spectrometer to facilitate a range of innovative studies of chemical and biochemical processes. Mass spectrometers operated with a thin permeable membrane over a vacuum inlet allow small gas molecules to be continuously monitored inside specific gas or liquid phase sample chambers. The proposed instrumentation will enable simultaneous and highly sensitive measurement of 10 different isot ....A multiple-ion membrane inlet mass spectrometer. There is a compelling need for a modern isotope-ratio mass spectrometer to facilitate a range of innovative studies of chemical and biochemical processes. Mass spectrometers operated with a thin permeable membrane over a vacuum inlet allow small gas molecules to be continuously monitored inside specific gas or liquid phase sample chambers. The proposed instrumentation will enable simultaneous and highly sensitive measurement of 10 different isotopic species and the capability for detection of hydrogen. This equipment will provide the many researchers and students with opportunities to determine isotopic signatures of isolated chemical reactions through to complex biochemical systems of whole living cells.Read moreRead less
Re-engineering rice root architecture to maximise water use efficiency. This project aims to discover gene networks responsible for producing deeper and more branched roots in rice plants. The roots of plants are the primary mechanism for absorbing water and nutrients from the soil. Manipulating roots to penetrate deeper with greater branching allows plants to thrive with less water and less nutrients. The project will identify key genes and proteins responsible for this process, and alter their ....Re-engineering rice root architecture to maximise water use efficiency. This project aims to discover gene networks responsible for producing deeper and more branched roots in rice plants. The roots of plants are the primary mechanism for absorbing water and nutrients from the soil. Manipulating roots to penetrate deeper with greater branching allows plants to thrive with less water and less nutrients. The project will identify key genes and proteins responsible for this process, and alter their expression in order to assess the role of these regulatory elements in root development in rice plants. The project expects to provide new, more sustainable varieties of rice which will help provide enhanced food security.Read moreRead less
Defining factors that influence protein turnover in plants. This project aims to discover how the functions of different proteins change as they age, and to define factors that dictate protein stability inside plants. This project will change protein turnover rates in plants by altering a regulator of this process to assess the role of this regulator in different plant developmental transitions. Expected outcomes include showing how protein abundance can be altered in plants for our future agric ....Defining factors that influence protein turnover in plants. This project aims to discover how the functions of different proteins change as they age, and to define factors that dictate protein stability inside plants. This project will change protein turnover rates in plants by altering a regulator of this process to assess the role of this regulator in different plant developmental transitions. Expected outcomes include showing how protein abundance can be altered in plants for our future agricultural and biotechnology needs. This will provide significant benefits, such as discovering processes inside plant cells that maintain the quality of different kinds of proteins and propose how different kinds of proteins can be stabilised for plant biotechnology applications.Read moreRead less
REdefining metabolic Schemes and Pathways In plant leaf REspiration. Leaf respiration-related metabolism in terrestrial vegetation liberates considerable amounts of carbon dioxide, ammonia and hydrogen sulphide into the atmosphere. Such gaseous losses are detrimental to biomass production but respiration also sustains nutrient assimilation and biosyntheses. This project aims to describe flux patterns in respiratory metabolism and disentangle interactions with other pathways such as photorespirat ....REdefining metabolic Schemes and Pathways In plant leaf REspiration. Leaf respiration-related metabolism in terrestrial vegetation liberates considerable amounts of carbon dioxide, ammonia and hydrogen sulphide into the atmosphere. Such gaseous losses are detrimental to biomass production but respiration also sustains nutrient assimilation and biosyntheses. This project aims to describe flux patterns in respiratory metabolism and disentangle interactions with other pathways such as photorespiration and nitrogen assimilation. It will exploit stable isotopes to quantify metabolic partitioning and show coordination between major processes. It will establish key mechanisms by which respiration dictates plant carbon balance and contributes to identifying metabolic bottle-necks in plant primary production.Read moreRead less
Smoke-derived karrikins reveal a new pathway for plant development. This project aims to investigate a recently discovered signalling system in plants that requires an unknown hormone to regulate seed germination and seedling growth. Burning vegetation produces karrikins that promote seed germination by signalling through a recently identified plant protein. Plants do not make karrikins and impairments to this protein results in increased seed dormancy and abnormal leaf growth, suggesting karrik ....Smoke-derived karrikins reveal a new pathway for plant development. This project aims to investigate a recently discovered signalling system in plants that requires an unknown hormone to regulate seed germination and seedling growth. Burning vegetation produces karrikins that promote seed germination by signalling through a recently identified plant protein. Plants do not make karrikins and impairments to this protein results in increased seed dormancy and abnormal leaf growth, suggesting karrikins mimic an unknown plant hormone that regulates these aspects of plant development. The project aims to identify this phytohormone and elucidate its biosynthetic pathway. The identification of this new plant growth regulator would be a major advance for plant science and may create new opportunities in restoration ecology, weed control and food production.Read moreRead less
From energy stress to hormones: new signals in bacteria and plants. This project will use molecular tools to detect and identify new chemical signals, known as butenolides, that regulate the growth and development of bacteria and plants. This project will use innovative, interdisciplinary techniques to discover where these butenolide signals come from, and how both bacteria and plants detect them. Expected outcomes of this project include a greater understanding of how plants use butenolides to ....From energy stress to hormones: new signals in bacteria and plants. This project will use molecular tools to detect and identify new chemical signals, known as butenolides, that regulate the growth and development of bacteria and plants. This project will use innovative, interdisciplinary techniques to discover where these butenolide signals come from, and how both bacteria and plants detect them. Expected outcomes of this project include a greater understanding of how plants use butenolides to cope with stress such as drought or salinity, and the design of new technologies for manipulating the growth of both plants and bacteria. The long-term benefits of this work should include fresh approaches for enhancing plant performance under sub-optimal conditions.Read moreRead less
Discovering the molecular mechanisms and origins of karrikin and strigolactone signalling in plants. Understanding how hormones control plant growth has transformed plant biology and driven major advances in crop production. This project will study genes responsible for the action of two new growth regulators, strigolactones and karrikins, and, by uncovering their action mechanisms, will obtain crucial knowledge to stimulate yet further advances in plant science.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100015
Funder
Australian Research Council
Funding Amount
$200,000.00
Summary
Purchase of an ultra-performance liquid chromatograph - triple quadrupole mass spectrometer. The diverse research supported by the proposed instrument group addresses several national research priorities. It will lead to a better fundamental understanding of the hormonal control of plant growth, improved catalysts for organic synthesis including pharmaceuticals and improved food safety. In forestry it will help to increase forest productivity through mitigating losses from insect and mamm ....Purchase of an ultra-performance liquid chromatograph - triple quadrupole mass spectrometer. The diverse research supported by the proposed instrument group addresses several national research priorities. It will lead to a better fundamental understanding of the hormonal control of plant growth, improved catalysts for organic synthesis including pharmaceuticals and improved food safety. In forestry it will help to increase forest productivity through mitigating losses from insect and mammalian pests and enhancing wood quality. In pharmaceutics, improved treatments for asthma are expected. This facility will provide the infrastructure essential for many researchers to maintain internationally competitive profiles in their areas and continue to offer postgraduate training and postdoctoral opportunities.Read moreRead less
How plants open up: revealing the evolution of stomatal opening mechanisms. This project aims to identify novel and conserved mechanisms that drive the opening of stomata – plant pores that enable CO2 acquisition for photosynthesis. Stomatal movements strongly affect plant productivity and water use efficiency and have profoundly influenced the earth’s climate and terrestrial ecology. This project will address critical gaps in our understanding of how plants open stomata in response to their env ....How plants open up: revealing the evolution of stomatal opening mechanisms. This project aims to identify novel and conserved mechanisms that drive the opening of stomata – plant pores that enable CO2 acquisition for photosynthesis. Stomatal movements strongly affect plant productivity and water use efficiency and have profoundly influenced the earth’s climate and terrestrial ecology. This project will address critical gaps in our understanding of how plants open stomata in response to their environment and the evolutionary history of the genes controlling this fundamental process. A major expected outcome is knowledge of the diversity of stomatal opening pathways, which should ultimately lead to improved predictions of plant responses to environmental change and assist future targeted modification of plant growth.Read moreRead less
Molecular switches and genetic consequences of grain retention in cereals. Grain retention at maturity was key for crop domestication and laid the basis for farming. Wheat and barley have evolved a novel mechanism for ensuring grain retention and, although the genes are known, the mechanisms for action are not. Grain dispersal in the wild relatives involves highly targeted changes in the walls of a small number of cells. This project will explore how the two identified genes control this proces ....Molecular switches and genetic consequences of grain retention in cereals. Grain retention at maturity was key for crop domestication and laid the basis for farming. Wheat and barley have evolved a novel mechanism for ensuring grain retention and, although the genes are known, the mechanisms for action are not. Grain dispersal in the wild relatives involves highly targeted changes in the walls of a small number of cells. This project will explore how the two identified genes control this process and clarify their mode of action. The genes ensuring grain retention have been so critical for domestication that the region surrounding them has become genetically fixed. The project will assess the implication of fixation on genetic diversity and develop options to bring novel variation into breeding programs.Read moreRead less