Protein modifications in plant mitochondria: towards functional proteomics. Energy production within plants in cellular structures called mitochondria is vital for their growth and development and is central to the early success of germinating and growing seedlings. This project intends to analyse mitochondria within plants using state of the art instrumentation and technologies. The findings from this research have the potential to directly flow into the plant biotechnology industry and could a ....Protein modifications in plant mitochondria: towards functional proteomics. Energy production within plants in cellular structures called mitochondria is vital for their growth and development and is central to the early success of germinating and growing seedlings. This project intends to analyse mitochondria within plants using state of the art instrumentation and technologies. The findings from this research have the potential to directly flow into the plant biotechnology industry and could assist the future development of Australian agriculture through genetic improvements. The expertise developed by this work will ensure that Australia is well placed to exploit future advances in this field and to further generate the development of novel biotechnological applications in agriculture.Read moreRead less
Plant Mitochondrial Signalling and Regulation. Plant energy production is essential for successful growth and development and is essential for processes such as seedling establishment and germination. This research project intends to expand our understanding of energy regulation within the plant using advanced technologies. Such studies can provide direct benefits to the Australian agriculture community through novel targets for genetic improvements. The capacity to create such advantages is eco ....Plant Mitochondrial Signalling and Regulation. Plant energy production is essential for successful growth and development and is essential for processes such as seedling establishment and germination. This research project intends to expand our understanding of energy regulation within the plant using advanced technologies. Such studies can provide direct benefits to the Australian agriculture community through novel targets for genetic improvements. The capacity to create such advantages is economically vital for the industry and the development of such expertise within Australia will ensure we are well placed to exploit future advances in agricultural improvements and provide the capacity to further generate novel biotechnological applications.Read moreRead less
Functional and structural characterisation of Defective embryo and meristems (Dem) proteins involved in plant development. The proposed research will lead to advances in understanding the regulation of plant development, a process impacting on agriculture, environmental management and human health, areas designated as national research priorities. This understanding is required for modifying plant growth and architecture to fit particular environments, for example generating plants with more ext ....Functional and structural characterisation of Defective embryo and meristems (Dem) proteins involved in plant development. The proposed research will lead to advances in understanding the regulation of plant development, a process impacting on agriculture, environmental management and human health, areas designated as national research priorities. This understanding is required for modifying plant growth and architecture to fit particular environments, for example generating plants with more extensive and deeper roots to mine the soil moisture and nutrients to enhance crop productivity in Australia, and maintaining the competitive advantage of Australian agriculture in view of the range of environmental conditions encountered in this country. The project will also contribute to the health of the Australian population through consumable plants in the diet.Read moreRead less
Aquaporins in roots: resolving observations linking them to diverse processes in water relations and plant productivity. The knowledge we gain will benefit Australia by allowing better management of plant water use and productivity. This is critical for adaptation to a drier climate where water is a critical resource. Large quantities of water move through aquaporin proteins in plants, therefore our understanding of these and the way they influence other processes in plant growth could enable us ....Aquaporins in roots: resolving observations linking them to diverse processes in water relations and plant productivity. The knowledge we gain will benefit Australia by allowing better management of plant water use and productivity. This is critical for adaptation to a drier climate where water is a critical resource. Large quantities of water move through aquaporin proteins in plants, therefore our understanding of these and the way they influence other processes in plant growth could enable us to manipulate plants to conserve water or to extract it more efficiently from the soil. Molecular aspects of the project could reveal new unexploited links between water and plant productivity. High calibre PhD and Honours students will also be educated to maintain the momentum of international excellence within Australia in the field of plant water relations.Read moreRead less
Active bicarbonate transporters from cyanobacteria: physiological properties, genetic regulation, and introduction into plants for crop improvement. An intriguing set of membrane transport proteins that accumulate bicarbonate into marine cyanobacterial cells will be investigated. These proteins support the crucial process of photosynthetic carbon dioxide fixation in marine cyanobacteria (blue-green algae), which are major contributors to global carbon dioxide sequestration and form one of the f ....Active bicarbonate transporters from cyanobacteria: physiological properties, genetic regulation, and introduction into plants for crop improvement. An intriguing set of membrane transport proteins that accumulate bicarbonate into marine cyanobacterial cells will be investigated. These proteins support the crucial process of photosynthetic carbon dioxide fixation in marine cyanobacteria (blue-green algae), which are major contributors to global carbon dioxide sequestration and form one of the foundations of the marine food web. These bicarbonate "transporters" will also be transferred into a model plant system to test whether the efficiency of photosynthesis can be improved, with corresponding gains in the water-use efficiency of these plants. If successful this technology will have profound global implications for improving crop production in semi-arid areas.Read moreRead less
Molecular basis of the antimicrobial activity of the floral defensin, NaD1, for the plant pathogen Fusarium oxysporum. Filamentous fungi are responsible for many major plant diseases that result in devastating crop losses and food spoilage world wide. Currently there are no resistant cultivars or adequate chemical controls for many of these diseases. The plant defensin, NaD1, stops the growth of many pathogens, including the recalcitrant fungi Fusarium oxysporum and Botrytis cinerea, and has bee ....Molecular basis of the antimicrobial activity of the floral defensin, NaD1, for the plant pathogen Fusarium oxysporum. Filamentous fungi are responsible for many major plant diseases that result in devastating crop losses and food spoilage world wide. Currently there are no resistant cultivars or adequate chemical controls for many of these diseases. The plant defensin, NaD1, stops the growth of many pathogens, including the recalcitrant fungi Fusarium oxysporum and Botrytis cinerea, and has been shown to protect transgenic cotton against fungal infection in glasshouse and field trials. NaD1 has potential application for durable, broad spectrum fungal disease control in crops. This will lead to both environmental and economic benefits to Australia. Read moreRead less
Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, ....Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, cytosolic pH and aluminium uptake across the plasma membrane in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop culti ....Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop cultivars are important part of sustainable farming in Australia. This project will characterise early triggers of Al toxicity in plants, providing a foundation for increasing Al resistance in crop cultivars. Understanding the physiological basis of Al toxicity will lead to improved crop breeding strategies.Read moreRead less
Defining the evolutionary processes of resistance to the new mode of action herbicide, pyroxasulfone. The sustainability of the Australin grains industry is threatened by the continuing evolution and widespread expansion of herbicide resistant weed populations across the crop production regions. The resulting loss in herbicide efficacy is forcing producers away from the environmentally friendly practices of stubble retention and reduced tillage in an effort to control herbicide resistant weed po ....Defining the evolutionary processes of resistance to the new mode of action herbicide, pyroxasulfone. The sustainability of the Australin grains industry is threatened by the continuing evolution and widespread expansion of herbicide resistant weed populations across the crop production regions. The resulting loss in herbicide efficacy is forcing producers away from the environmentally friendly practices of stubble retention and reduced tillage in an effort to control herbicide resistant weed populations. This research is aimed at conserving a novel mode of action herbicide with efficacy on resistant Lolium rigidum populations. The success of this project will inevitably lead the pesticide industry to adopt this approach for future product development. Read moreRead less
Carboxylate exudation and phosphate nutrition in Hakea prostrata (Proteaecea). Nonmycorrhizal Proteaceae are very successful in acquiring phosphate from nutrient-impoverished soils; their cluster roots account for this. They are also extremely sensitive to phosphate toxicity. We will elucidate aspects of production and release of carboxylates that are associated with functioning of cluster roots, using Hakea prostrata (Proteaceae) as a model. Types and rates of exudation by cluster roots, as in ....Carboxylate exudation and phosphate nutrition in Hakea prostrata (Proteaecea). Nonmycorrhizal Proteaceae are very successful in acquiring phosphate from nutrient-impoverished soils; their cluster roots account for this. They are also extremely sensitive to phosphate toxicity. We will elucidate aspects of production and release of carboxylates that are associated with functioning of cluster roots, using Hakea prostrata (Proteaceae) as a model. Types and rates of exudation by cluster roots, as influenced by development and environmental signals, will be assessed. Our findings will provide key information on adaptive mechanisms associated with both phosphate acquisition from phosphate-fixing soils and phosphate toxicity.Read moreRead less