Role Of RNA-binding Proteins In Cardiomyocyte Physiology
Funder
National Health and Medical Research Council
Funding Amount
$880,494.00
Summary
Interactions between ribonucleic acids and proteins are of critical importance to gene expression and may also connect it to cell metabolism in unexpected ways. We hypothesise that this is of particular importance in cardiac health and disease. We will employ the tools of proteomics and transcriptomics to characterise the topology and function of RNA-protein interaction networks in heart muscle cells, and thus contribute to the search for better treatment.
Charting The Interface Between Cellular Metabolic States And Gene Regulation
Funder
National Health and Medical Research Council
Funding Amount
$653,196.00
Summary
The research successes of Molecular Biology and Biochemistry have given us detailed pictures of the regulatory and metabolic states of cells and tissues, yet we know little about how these states affect each other. We hypothesise the existence of regulatory interactions between ribonucleic acids, enzymes and metabolites to connect gene expression and metabolism. We will employ novel RNA Biology methods to discover such regulatory interactions in medically important cellular contexts.
Nuclear Sirtuins At The Interface Between Epigenetic Regulation And Human Cytomegalovirus Pathogenesis: A Proteomics Perspective
Funder
National Health and Medical Research Council
Funding Amount
$399,488.00
Summary
Human cytomegalovirus (HCMV) is a human pathogen that infects over 60% of the adult population, and is a major cause of birth defects causing permanent hearing and vision loss, and mental retardation. To investigate the critical involvement of host epigenetic factors, I will study the roles of sirtuins during HCMV infection. Through proteomic, genomic, microscopy, and bioinformatic techniques, I aim to further our understanding of viral pathogenesis, towards developing novel therapies.
Decision-making modules in protein interaction networks. This project aims to discover how cells use proteins to make decisions. This is important for all living things, which must react to stimuli to grow, adapt, defend themselves and to die. The project’s anticipated outcome is the systems-level identification of decision-making modules in an intracellular network. Its focus is on the smallest possible modules, which contain a decision-making protein with two modifications that control protein ....Decision-making modules in protein interaction networks. This project aims to discover how cells use proteins to make decisions. This is important for all living things, which must react to stimuli to grow, adapt, defend themselves and to die. The project’s anticipated outcome is the systems-level identification of decision-making modules in an intracellular network. Its focus is on the smallest possible modules, which contain a decision-making protein with two modifications that control protein-proteins interactions. It will investigate two recurrent decision-making modules. The expected benefits of the project include new means to decipher biological complexity, and targets to modulate biosystems by genome editing or with drugs.Read moreRead less
The role and regulation of protein methylation: a study using the recently developed methylation network of yeast. Tiny changes to proteins, such as methylation, can alter the way they interact with other proteins. This project will investigate the dynamics of protein methylation during the life of the yeast cell. The project results will be of long term relevance to situations where we may want to stop cells dividing, such as cancer or infectious disease.
The effect of methylation and phosphorylation on ribosome function. This project aims to discover how cells regulate ribosome function and selectivity, by modifying their ribosomal proteins. This affects protein synthesis, a process which is central to the growth of all living things. Expected outcomes include new knowledge on the regulation of protein synthesis, improved techniques for the study of this process and an enhanced capacity for international collaboration. New avenues for the artifi ....The effect of methylation and phosphorylation on ribosome function. This project aims to discover how cells regulate ribosome function and selectivity, by modifying their ribosomal proteins. This affects protein synthesis, a process which is central to the growth of all living things. Expected outcomes include new knowledge on the regulation of protein synthesis, improved techniques for the study of this process and an enhanced capacity for international collaboration. New avenues for the artificial regulation of the ribosome may also emerge, relevant to synthetic biology and the engineering of industrial yeasts. The project should provide significant new findings for the research community, generate research citations and contribute to a highly skilled workforce by the training of staff and students.Read moreRead less
Does phosphorylation regulate the methylation of proteins? . The interaction of proteins is a fundamental requirement of life. Tiny switches on proteins affect how they interact but little is known about how these are controlled. This project will study the complex interplay between two types of switches; one is expected to control the other. This will provide new insights into how the cell functions.
Discovery Early Career Researcher Award - Grant ID: DE150100019
Funder
Australian Research Council
Funding Amount
$352,000.00
Summary
Protein network regulation: A systems-level analysis of methylation. The systems-level analysis of protein-protein interaction networks is a vital new framework for exploring protein complex formation. However key studies into the dynamics of these networks are being precluded by a lack of broad-scale data on interactions mediated by post-translational modifications, which are known regulators of protein-protein interactions. To solve this problem, this project aims to conduct an organism-wide s ....Protein network regulation: A systems-level analysis of methylation. The systems-level analysis of protein-protein interaction networks is a vital new framework for exploring protein complex formation. However key studies into the dynamics of these networks are being precluded by a lack of broad-scale data on interactions mediated by post-translational modifications, which are known regulators of protein-protein interactions. To solve this problem, this project aims to conduct an organism-wide survey of interactions mediated by an important class of post-translational modification, methylation, using an innovative mass spectrometry-based workflow. This project aims to produce fundamental new insights into the mechanisms underlying protein-protein interaction network dynamics, and into how protein complexes are formed.Read moreRead less
Identifying The Molecular Mechanisms Of Synergistic And Antagonistic Drug-drug Interactions In Combination Chemotherapies
Funder
National Health and Medical Research Council
Funding Amount
$412,685.00
Summary
Drug combinations in chemotherapy hold promise for more effective treatments and for overcoming drug resistance, but the search for effective combinations is challenging. The combination therapy R-CHOP is often curative for Diffuse Large B-cell Lymphoma (DLBCL). Both cellular drug interactions and evolutionary drug interactions will be quantified in DLBCL cells, to understand the defining features of effective combinations and guide the future rational design of combinations.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100204
Funder
Australian Research Council
Funding Amount
$550,000.00
Summary
A systems biology capability for the Ramaciotti Centre for Genomics. A systems biology capability for the Ramaciotti Centre for Genomics:
The project aims to introduce a complete systems biology capability to the Ramaciotti Centre for Genomics. This is intended to provide a complete systems biology workflow, including improved data analysis for next-generation sequencing, tissue preparation and imaging, and mass spectrometry for proteomics and metabolomics. This would enable a truly systems app ....A systems biology capability for the Ramaciotti Centre for Genomics. A systems biology capability for the Ramaciotti Centre for Genomics:
The project aims to introduce a complete systems biology capability to the Ramaciotti Centre for Genomics. This is intended to provide a complete systems biology workflow, including improved data analysis for next-generation sequencing, tissue preparation and imaging, and mass spectrometry for proteomics and metabolomics. This would enable a truly systems approach to biological problems, supporting researchers and projects that focus on microbial and mammalian metabolism, carbohydrate chemistry and synthetic biology. Read moreRead less