Involvement of cell coupling in vascular function: Development of a computational model. Gap junctions are intercellular channels which enable the production of coordinated responses in multicellular tissues and organs. Blood vessels are comprised of endothelial cells surrounded by smooth muscle cells and gap junctions exist within and between these layers. The present proposal will determine the fundamental role of gap junctions in regulating blood flow and blood pressure. Our data will enable ....Involvement of cell coupling in vascular function: Development of a computational model. Gap junctions are intercellular channels which enable the production of coordinated responses in multicellular tissues and organs. Blood vessels are comprised of endothelial cells surrounded by smooth muscle cells and gap junctions exist within and between these layers. The present proposal will determine the fundamental role of gap junctions in regulating blood flow and blood pressure. Our data will enable us to develop a computational model of the vascular wall and so predict how changes in electrical properties, as occur during pressure changes, can influence blood flow. Since ageing is accompanied by an increase in blood pressure, our results will contribute to a better understanding of blood flow regulation in our ageing population.Read moreRead less
Cellular and network basis of information processing in the mammalian visual system. The project aims to discover the strategies by which cells in the brain interact with each other to code the sensory input efficiently. It is expected that simultaneous recording of the activity of many neurones from the visual cortex of anaesthetised cats during visual stimulation will reveal how the biophysics of synaptic integration combined with excitatory and inhibitory inputs from different sources sculpt ....Cellular and network basis of information processing in the mammalian visual system. The project aims to discover the strategies by which cells in the brain interact with each other to code the sensory input efficiently. It is expected that simultaneous recording of the activity of many neurones from the visual cortex of anaesthetised cats during visual stimulation will reveal how the biophysics of synaptic integration combined with excitatory and inhibitory inputs from different sources sculpts the output of individual neurones. The experiments will be extended to the study of possible interactions between different areas of the brain and the study of mechanisms by which the cortical network and higher cognitive factors such as attention and memory might influence the coding of sensory information in awake animals.Read moreRead less
Unified framework of intestinal motility. The project aims to establish how a few fundamental mechanisms determine the large repertoire of intestinal motor patterns responsible for moving nutrients along the digestive tract. The project will combine experimental and theoretical data, with biomechanical and electrophysiological models to create a new understanding of this essential function of the body.
REGULATION OF RYANODINE RECEPTOR CALCIUM CHANNELS BY THE CALCIUM BINDING PROTEIN CALSEQUESTRIN. The project is to examine the functional interaction between two proteins in skeletal muscle that are essential for Ca2+ regulation and hence contraction, respiration and movement in all vertebrate species. One protein, the ryanodine receptor, releases calcium from stores inside the muscle cell. The other protein, calsequestrin, binds and sequesters calcium ions. We have recently discovered that th ....REGULATION OF RYANODINE RECEPTOR CALCIUM CHANNELS BY THE CALCIUM BINDING PROTEIN CALSEQUESTRIN. The project is to examine the functional interaction between two proteins in skeletal muscle that are essential for Ca2+ regulation and hence contraction, respiration and movement in all vertebrate species. One protein, the ryanodine receptor, releases calcium from stores inside the muscle cell. The other protein, calsequestrin, binds and sequesters calcium ions. We have recently discovered that the proteins also bind to each other and that calsequestrin regulates Ca2+ release from the stores through the ryanodine receptor ion channel. This regulation is likely to be important in conserving store calcium during stress or fatigue.Read moreRead less
Suction pipette measurements of mammalian rod photoreceptor recovery following intense bleaching exposures. The aim of this project is to discover the events and processes that prevent retinal photoreceptors from recovering instantaneously following the cessation of exposure to extremely bright illumination. Recordings will be made from single rod photoreceptors cells isolated from the mammalian retina. The work will uncover the relative roles of the 'photoproducts' created when rhodopsin abso ....Suction pipette measurements of mammalian rod photoreceptor recovery following intense bleaching exposures. The aim of this project is to discover the events and processes that prevent retinal photoreceptors from recovering instantaneously following the cessation of exposure to extremely bright illumination. Recordings will be made from single rod photoreceptors cells isolated from the mammalian retina. The work will uncover the relative roles of the 'photoproducts' created when rhodopsin absorbs light: e.g. intermediates such as metarhodopsin and opsin. The molecular knowledge obtained will help us to understand why it is that the visual system recovers so slowly after the eye has experienced very intense light.Read moreRead less
The first stage of vision: transduction and adaptation in retinal photoreceptors. The project aims to provide a detailed understanding of the molecular steps involved in the first stage of vision - the conversion of light into a neural signal in the rod and cone photoreceptors of the retina. The significance of this is that it will explain the initial events that enable us to see, and will help explain the deficits that occur when the process fails. The outcome will be a comprehensive understand ....The first stage of vision: transduction and adaptation in retinal photoreceptors. The project aims to provide a detailed understanding of the molecular steps involved in the first stage of vision - the conversion of light into a neural signal in the rod and cone photoreceptors of the retina. The significance of this is that it will explain the initial events that enable us to see, and will help explain the deficits that occur when the process fails. The outcome will be a comprehensive understanding of how our photoreceptors respond with extreme sensitivity, yet great rapidity, and over an enormous range of light intensities, thus endowing us with our remarkable sense of vision.Read moreRead less
How triadin and junctin communicate with ryanodine receptors deep within a calcium store to determine skeletal muscle contraction. The project results will provide a platform for muscle relaxants and other drugs that will specifically target either the heart or skeletal muscle and will have applications in the livestock, veterinary and pharmaceutical Industries. The project falls within the National Research Priorities of Promoting and Maintaining Good Health and Frontier Technologies for Buil ....How triadin and junctin communicate with ryanodine receptors deep within a calcium store to determine skeletal muscle contraction. The project results will provide a platform for muscle relaxants and other drugs that will specifically target either the heart or skeletal muscle and will have applications in the livestock, veterinary and pharmaceutical Industries. The project falls within the National Research Priorities of Promoting and Maintaining Good Health and Frontier Technologies for Building and Transforming Australian Industries, as well as the national priority goal of Ageing well, Ageing Productively. The project will be of national benefit in training undergraduate students, PhD students and a postdoctoral fellow in state-of-the-art techniques in an internationally competitive research field.Read moreRead less
Aquafin CRC - Atlantic Salmon Aquaculture Subprogram: Treatment And Pathophysiology Of Amoebic Gill Disease
Funder
Fisheries Research and Development Corporation
Funding Amount
$659,515.00
Summary
There is an urgent need to develop novel treatments which would reduce the impact of AGD on salmon industry. A detailed benefit – cost analysis for the AQUAFIN CRC AGD project was undertaken which gave a Net Present Value of the economic benefit of $21.6M, Benefit/Cost Ratio of 5.3. The current project will deliver the short-term solutions and provide information and advice to the industry on a regular basis.
The current project is part of an integrated research program, with delivera ....There is an urgent need to develop novel treatments which would reduce the impact of AGD on salmon industry. A detailed benefit – cost analysis for the AQUAFIN CRC AGD project was undertaken which gave a Net Present Value of the economic benefit of $21.6M, Benefit/Cost Ratio of 5.3. The current project will deliver the short-term solutions and provide information and advice to the industry on a regular basis.
The current project is part of an integrated research program, with deliverables not only aligned with the development of novel treatments, but also with effective vaccine development, management and other control methods. Developing and maintaining standarised infection is included in this subproject, and it will be crucial for vaccine testing and experimental work within the Host-pathogen interaction project and the Model development – epidemiology of AGD project. The development of a quantitative experimental challenge model is an essential prerequisite to vaccine research and novel treatment testing. Without the quantitative experimental model it is very difficult to compare effectiveness of treatments or vaccines tested in separate experiments. Additionally, the controlled lab infection will provide crude gill isolates to researchers working in other projects.
The growth of the industry and ineffectiveness of freshwater bathing has resulted in the need for the development of new treatments that will either aid in improving current freshwater bathing technology, or offer completely new avenues for the treatment of AGD in Atlantic salmon. Effective treatment of the disease can only be achieved if we understand the physiological and pathological processes at work. To this end it is imperative that we understand:
1. The effect of the parasite on the normal physiology of the salmon so resulting in mortality 2. The effect of the treatment on the parasite 3. The effects of the treatment on fish parasitised to different degrees (I.e., potential toxicity side effects of treatments)
Successful treatments will be rapidly adopted by the industry given the problems with freshwater bathing technology. Advances in the development of either additives to the current freshwater baths or freshwater bath replacement by a less time-consuming chemical treatment will ensure that treatment and control of AGD as a constraint to Atlantic salmon production in Tasmania is eliminated. Objectives: 1. Establish an on-going laboratory source of AGD affected fish. 2. Establish and validate controlled infection/challenge system. 3. To understand how AGD affects the respiratory and cardiovascular systems of Atlantic salmon. 4. To determine how environmental parameters interact with AGD pathophysiology. In particular the interaction of temperature, oxygen, salinity, and carbon dioxide. 5. To develop and test new chemical or pharmaceutical treatments for the control of AGD. 6. To optimise the efficacy of current treatments by minimising the physiological impact on the fish. Read moreRead less
SCRC: SCRC Honours Scholarship H4.3 The Effects Of Feeding Alternative Protein Sources On The Intestinal Mucus Layer And Mucosal Architecture In Yellowtail Kingfish (Rebecca Forder; Student Matthew Bransemer.)
Sensory integration in the whisker pathway: cortical transformations and the neuronal mechanisms that underlie decision confidence. How does the brain use information collected by sensory systems to make decisions? This study combines behavioural experiments with neuronal recording from various brain regions to understand the neuronal mechanisms that integrate sensory signals to generate representations and guide the appropriate behavioural action.