Techniques for probing biological media with holographic angular scattering spectroscopy. Huge effort in the biological and medical sciences is spent in using simple software to laboriously mark, count and measure cells and structures in microscope images of samples. We could replace this incredibly inefficient process in many instances with single-shot size-map images of unstained samples. If depth selectivity can be added, we could perform non-invasive measurements on animals, making a huge re ....Techniques for probing biological media with holographic angular scattering spectroscopy. Huge effort in the biological and medical sciences is spent in using simple software to laboriously mark, count and measure cells and structures in microscope images of samples. We could replace this incredibly inefficient process in many instances with single-shot size-map images of unstained samples. If depth selectivity can be added, we could perform non-invasive measurements on animals, making a huge reduction in their usage. We could assess thick tissues enabling early noninvasive diagnosis of malignancy in tumours in situ or muscle characterization for meat quality or muscular dystrophy. These benefits impact on research, animal ethics, and on health, and have commercial potential in life and medical sciences, and the meat industry.Read moreRead less