Subcellular co-localisation of interacting proteins that control maturation in mammalian eggs. The final maturation of mammalian eggs (oocytes) before fertilisation involves a cascade of interactions between protein kinases and phosphatases, the shuttling of these proteins between cytoplasm and nucleus, and microtubule assembly and disassembly. In this project we have proposed that interacting proteins involved in oocyte maturation are co-localised at subcellular sites in oocytes, in a strict t ....Subcellular co-localisation of interacting proteins that control maturation in mammalian eggs. The final maturation of mammalian eggs (oocytes) before fertilisation involves a cascade of interactions between protein kinases and phosphatases, the shuttling of these proteins between cytoplasm and nucleus, and microtubule assembly and disassembly. In this project we have proposed that interacting proteins involved in oocyte maturation are co-localised at subcellular sites in oocytes, in a strict temporal and spatial manner. The co-localisation of proteins in oocytes is considered a fundamental mechanism that ensures coordination of time-bound cellular events and proper preparation of oocytes for fertilisation and early embryo development. There are important implications for IVF and cloning by nuclear transfer.Read moreRead less