Development of ozone-induced dissociation for lipidomics workflows. An Australian invention (ozone induced dissociation) will be developed in collaboration with a major instrument manufacturer. This project will provide Australian researchers with unique capabilities to investigate the role of lipids (fats) in human disease and will place them at the forefront of lipid research internationally.
Discovery Early Career Researcher Award - Grant ID: DE120100467
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Unravelling the intrinsic structure and stability of multiply charged anions in the gas-phase using photoelectron spectroscopy and mass spectrometry. Molecules possessing multiple negative charges are common constituents in chemistry, influencing a range of processes ranging from photochemical smog formation to protein structure in vivo. This project will develop new technologies to probe their molecular structure in the gas-phase, leading to a more rigorous understanding of these species.
The photons take charge: Elucidating the structure and stability of distonic radical anions by mass spectrometry and photoelectron spectroscopy. Recent work has discovered that certain radical anions have electronic configurations that defy chemical convention and exhibit exceptional radical stability. Exploitation of this breakthrough first requires experimental elucidation of the intrinsic electronic structure of these compounds and how it relates to their remarkable properties. This project w ....The photons take charge: Elucidating the structure and stability of distonic radical anions by mass spectrometry and photoelectron spectroscopy. Recent work has discovered that certain radical anions have electronic configurations that defy chemical convention and exhibit exceptional radical stability. Exploitation of this breakthrough first requires experimental elucidation of the intrinsic electronic structure of these compounds and how it relates to their remarkable properties. This project will probe the fundamental structure and energetics of radical anions by modifying instrumentation to enable multi-step gas-phase ion synthesis to be efficiently coupled with anion photoelectron spectroscopy. These investigations are essential to revealing the scope of this phenomenon in free radical chemistry and biology and could inform future development of new catalysts for polymerisation.Read moreRead less
New laser and mass spectrometry-based tools for comprehensive structural elucidation of lipids and their biomolecular interactions. Lipid-related disorders such as obesity, diabetes and heart disease are reaching epidemic proportions. We propose the development of specialised instrumentation to identify and quantify lipids (fats) in tissue and culture samples thus providing Australian scientists with unique capabilities to investigate the mechanisms of disease.
Correction of non-linearity in inductively-coupled-plasma mass-spectrometry. Chemical analyses by mass spectrometers underpin key Australian economic sectors, particularly minerals and agriculture. The quadrupole inductively-coupled-plasma mass-spectrometer has seen a particular rise in prominence over last 25 years. In this collaboration between mass spectrometrists and the leading instrument designer, we will improve the linearity of its detection system for more precise and accurate data. Bet ....Correction of non-linearity in inductively-coupled-plasma mass-spectrometry. Chemical analyses by mass spectrometers underpin key Australian economic sectors, particularly minerals and agriculture. The quadrupole inductively-coupled-plasma mass-spectrometer has seen a particular rise in prominence over last 25 years. In this collaboration between mass spectrometrists and the leading instrument designer, we will improve the linearity of its detection system for more precise and accurate data. Better elemental and isotope ratio data from these high-throughput instruments will open up new real-world applications in many areas of Australian interest, such as biosecurity, forensics, groundwater management, and drug design. The research will also inform design of the next generation instruments by the industry partner.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100059
Funder
Australian Research Council
Funding Amount
$220,000.00
Summary
Multiplexed capabilities for surface analysis and imaging by mass spectrometry. This facility will support research aimed at developing rapid and reliable analytical methods for the detection of chemicals directly from biological and man-made materials. The mass spectroscopy methods used at the facility will reveal molecular-level changes in systems ranging from the lens of the human eye to Colorbond steel® and have applications in the detection of chemical and biological hazards.
Quantum noise limited molecular spectrometry. This project will develop a new technology for chemical analysis using lasers. The research will produce more accurate instruments for analysing samples containing carbon dioxide and water. This technology has a surprisingly wide array of applications. For example, sensitive analysis of carbon dioxide will help law enforcement agencies identify the location of illicit drug manufacturing, test for performance enhancing drug use by elite athletes, and ....Quantum noise limited molecular spectrometry. This project will develop a new technology for chemical analysis using lasers. The research will produce more accurate instruments for analysing samples containing carbon dioxide and water. This technology has a surprisingly wide array of applications. For example, sensitive analysis of carbon dioxide will help law enforcement agencies identify the location of illicit drug manufacturing, test for performance enhancing drug use by elite athletes, and monitor greenhouse gases. The instrument for analysing water will improve water resource management in Australia. This program will result in commercial instruments that are sensitive, portable and affordable.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100213
Funder
Australian Research Council
Funding Amount
$150,000.00
Summary
Advanced fluorescence characterisation facility. Fluorescence is the emission of light by a substance that has absorbed light of a different wavelength. Fluorescence techniques and fluorescent molecules have enabled a great many of the most important advances in biology, chemistry and medicine in recent decades. This facility will support a wide range of research projects using or exploring fluorescence and luminescence. The research supported will underpin advances in diverse scientific fields.
Microfluidic Separation Science: Innovative Technology for Characterising Complex Chemical Systems. At present there is a need for fast and detailed chemical analysis of complex samples, such as those important to biomedical diagnostics and forensic science. Innovative technology will be developed here in order to reduce analysis time whilst maintaining the integrity of the chemical information contained within the sample. This step change in separation science will directly aid biomedical diagn ....Microfluidic Separation Science: Innovative Technology for Characterising Complex Chemical Systems. At present there is a need for fast and detailed chemical analysis of complex samples, such as those important to biomedical diagnostics and forensic science. Innovative technology will be developed here in order to reduce analysis time whilst maintaining the integrity of the chemical information contained within the sample. This step change in separation science will directly aid biomedical diagnostics, forensic sample determination and industrial process monitoring through decreased analysis time with an increase in the chemical information gained. By performing chemical separations on a microfluidic scale a reduction in both the cost of analysis and impact of solvent waste on the environment will be achieved.Read moreRead less
Enhanced sensitivity of electrospray ionization mass spectrometry. Enhanced sensitivity of electrospray ionization mass spectrometry. This project aims to enhance the sensitivity of nano-electrospray ionization mass spectrometry (nanoESI-MS) by an order of magnitude by simultaneously overcoming the two interdependent limitations in ion generation and transmission efficiency. This project will design glass capillaries and tubes with complex structures to enable both multiplexing ion generation fr ....Enhanced sensitivity of electrospray ionization mass spectrometry. Enhanced sensitivity of electrospray ionization mass spectrometry. This project aims to enhance the sensitivity of nano-electrospray ionization mass spectrometry (nanoESI-MS) by an order of magnitude by simultaneously overcoming the two interdependent limitations in ion generation and transmission efficiency. This project will design glass capillaries and tubes with complex structures to enable both multiplexing ion generation from a single capillary and geometrically matching the bore of the tube collecting the emitted ion plume. NanoESI-MS has become an indispensable analytical tool for proteomics and synthetic chemistry. The significant enhancement of nanoESI-MS sensitivity in this project is expected to accelerate progress in disease research, biomarker discovery and drug development.Read moreRead less