Special Research Initiatives - Grant ID: SR0354702
Funder
Australian Research Council
Funding Amount
$10,000.00
Summary
Australian Microbial Resources Research Network. The Australian Microbial Resources Research Network will provide integrated access to Australian collections of microorganisms and electronic access to bioinformation databases to meet national strategic needs for microbiological resources and to support the competitive development of the life sciences and biotechnology industries in Australia. The network will promote collaborative interactions and accelerate the discovery of Australian microorg ....Australian Microbial Resources Research Network. The Australian Microbial Resources Research Network will provide integrated access to Australian collections of microorganisms and electronic access to bioinformation databases to meet national strategic needs for microbiological resources and to support the competitive development of the life sciences and biotechnology industries in Australia. The network will promote collaborative interactions and accelerate the discovery of Australian microorganisms and microbial genomic information for innovative biotechnology and create new opportunities for bioindustries. The Network will link researchers and foster the discovery and exploitation of Australian microbial resources and make these resources and associated information available for applications in research, industry and education.Read moreRead less
In vitro expression and crystallization of proteins from the thermohalophile, Halothermothrix orenii. The project proposal is to develop enabling state-of-the-art protein technologies for biotechnology. We will for the first time develop a rapid and high throughput technique for the expression, purification and crystallisation of proteins from the extreme thermohalophile, Halothermothrix orenii. This important break through in protein technology will assist in determining structure and function ....In vitro expression and crystallization of proteins from the thermohalophile, Halothermothrix orenii. The project proposal is to develop enabling state-of-the-art protein technologies for biotechnology. We will for the first time develop a rapid and high throughput technique for the expression, purification and crystallisation of proteins from the extreme thermohalophile, Halothermothrix orenii. This important break through in protein technology will assist in determining structure and function relationships of thermohalophilic proteins. It is expected that such an understanding together with comparative bioinformatics will assist researchers to develop "designer proteins" with improved functions for different uses in biotechnology. It is also expected that this technology will be amenable for use with other thermohalophilic microbes.Read moreRead less
Mechanisms of virus transport in indoor environments. The socio-economic benefits to Australia from the project will include the developed and validated model for quantification of virus spread and survival through aerosolation processes, which will become an important tool for: (i) prediction of the pathways of virus spread in indoor environment, and (ii) developing future directions for management and control for prevention or minimization the likelihood of human infections. The ultimate econo ....Mechanisms of virus transport in indoor environments. The socio-economic benefits to Australia from the project will include the developed and validated model for quantification of virus spread and survival through aerosolation processes, which will become an important tool for: (i) prediction of the pathways of virus spread in indoor environment, and (ii) developing future directions for management and control for prevention or minimization the likelihood of human infections. The ultimate economic benefit of this research will be reduction in health care costs and lost productivity. The research will also place Australia in the forefront of international progress and race towards toward better methods for virus spread prevention. Read moreRead less
Development of redox-mediated microbial assays for the rapid characterisation and assessment of wastewater, wastewater treatment processes and recycled water. With SE Qld on Level 5 water restrictions the need to reduce our demand on drinking water by increasing our reliance on recycled water is now urgent. To achieve this we need to ensure that the quality of our recycled wastewater is of a suitable and reliable standard. This project will assist the water industry in this endeavour by the de ....Development of redox-mediated microbial assays for the rapid characterisation and assessment of wastewater, wastewater treatment processes and recycled water. With SE Qld on Level 5 water restrictions the need to reduce our demand on drinking water by increasing our reliance on recycled water is now urgent. To achieve this we need to ensure that the quality of our recycled wastewater is of a suitable and reliable standard. This project will assist the water industry in this endeavour by the developing monitoring techniques that can rapidly characterise and assess the effectiveness of wastewater treatment processes and the quality of the water derived from them. These methods will provide early warnings of potential 'upsets' in treatment plants that would otherwise result in poor quality effluents that would need to be discharged as waste rather than be recycled.Read moreRead less
Early evolutionary origins of mechanosensory transduction: structure, function and phylogenetic studies of the family of mechanosensitive channels in cell-walled organisms. The proposed project is based on the recent progress the applicants have made by (i) identifying a novel family of mechanosensitive (MS) channels in cell-walled organisms, which include Archaea and Bacteria (prokaryotes), as well as fungi and plants (eukaryotes), and (ii) determining basic aspects of the structural dynamics ....Early evolutionary origins of mechanosensory transduction: structure, function and phylogenetic studies of the family of mechanosensitive channels in cell-walled organisms. The proposed project is based on the recent progress the applicants have made by (i) identifying a novel family of mechanosensitive (MS) channels in cell-walled organisms, which include Archaea and Bacteria (prokaryotes), as well as fungi and plants (eukaryotes), and (ii) determining basic aspects of the structural dynamics of MscL, the prototype MS channel, using electronparamagnetic resonance spectroscopy and molecular dynamics simulations in combination with the patch clamp technique. The aim of the project is to elucidate the molecular principles underlying the function of MS channels, which is of fundamental importance for understanding of the biophysics and physiology of mechanosensory transduction throughout the biological world.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453608
Funder
Australian Research Council
Funding Amount
$579,230.00
Summary
Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities. We seek to establish an Australian first confocal laser scanning microscope with time resolved imaging and fluorescence correlation spectroscopy capabilities. Its advantages include ultrasensitive detection of weak fluorescent emissions against high autofluorescent background by using fluorescence lifetime signatures, with over three orders of magnitude discrimi ....Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities. We seek to establish an Australian first confocal laser scanning microscope with time resolved imaging and fluorescence correlation spectroscopy capabilities. Its advantages include ultrasensitive detection of weak fluorescent emissions against high autofluorescent background by using fluorescence lifetime signatures, with over three orders of magnitude discrimination improvement. The system will also be able to monitor binding of single molecules. These techniques will open new and exciting avenues for interdisciplinary research at the frontier between biological and physical sciences. The microscope will operate within an existing multi-user Optical Characterisation Facility supporting research of an established network of scientists in the Sydney area.Read moreRead less
Ecosystem response to human, veterinary and growth promoting antibiotics in the aquatic environment. The objective of this study is to assess the threat to Australian aquatic ecosystems by antibiotics excreted and/or discarded through human activities each year. Specifically this study aims to: 1) Determine types of antibiotics, concentrations and distribution in surface waters downstream from potential sources; 2) Investigate bacterial resistance and bacterial community changes to antibiotic c ....Ecosystem response to human, veterinary and growth promoting antibiotics in the aquatic environment. The objective of this study is to assess the threat to Australian aquatic ecosystems by antibiotics excreted and/or discarded through human activities each year. Specifically this study aims to: 1) Determine types of antibiotics, concentrations and distribution in surface waters downstream from potential sources; 2) Investigate bacterial resistance and bacterial community changes to antibiotic compounds entering the aquatic environment; 3) Determine the influence of antibiotics on key ecological processes performed by bacteria - e.g. nitrogen cycle; 4) Assess effect on, and efficiency of, various effluent treatments in removing antibiotics and direct improvements for future removal.Read moreRead less
Sulfur cycling in soil environments - how bacteria contribute to the oxidation of organic and inorganic sulfur compounds. Element cycling in soil environments is of global significance as soils constantly exchange compounds with the atmosphere and cover vast areas of land. Many of the compounds exchanged are known contributors to the greenhouse effect and other phenomena such as acid rain. By elucidating the regulation of bacterial sulfur oxidation pathways and their integration into general met ....Sulfur cycling in soil environments - how bacteria contribute to the oxidation of organic and inorganic sulfur compounds. Element cycling in soil environments is of global significance as soils constantly exchange compounds with the atmosphere and cover vast areas of land. Many of the compounds exchanged are known contributors to the greenhouse effect and other phenomena such as acid rain. By elucidating the regulation of bacterial sulfur oxidation pathways and their integration into general metabolism, we will enable the development of better management strategies for agricultural soils. Our data will also significantly improve understanding of how soil processes will change in response to changing climatic conditions.Read moreRead less
Environmental controls over Fe availability and transport in a forested coastal catchment. Iron is one of a number of elements that are mobilised in catchments, have recognised detrimental impacts to marine settings and are known to be nutrients for toxic cyanobacteria blooms. Knowledge of controls over distribution of Fe is important in catchment management and for sustainable forestry. The total catchment approach will result in an understanding of release and transport of Fe, and other heav ....Environmental controls over Fe availability and transport in a forested coastal catchment. Iron is one of a number of elements that are mobilised in catchments, have recognised detrimental impacts to marine settings and are known to be nutrients for toxic cyanobacteria blooms. Knowledge of controls over distribution of Fe is important in catchment management and for sustainable forestry. The total catchment approach will result in an understanding of release and transport of Fe, and other heavy metals. The outcomes of the study will enable useful comparison to coastal plantations elsewhere in Australia, and overseas. Determination of the relationship to rainfall and hydrological processes will enhance these comparisons plus consideration of climatic change.
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An integrated genomics approach to improve our understanding of the biology of genital campylobacteriosis in beef cattle. Beef is Australia's most valuable agricultural export estimated at $9.6B annually and this industry accounts for one-third of full time employment in regional communities. Currently, unknown causes of reproductive losses in cattle in Northern Australian cost producers approximately $15M per annum. Bovine genital campylobacteriosis is one of the major risk factors associated w ....An integrated genomics approach to improve our understanding of the biology of genital campylobacteriosis in beef cattle. Beef is Australia's most valuable agricultural export estimated at $9.6B annually and this industry accounts for one-third of full time employment in regional communities. Currently, unknown causes of reproductive losses in cattle in Northern Australian cost producers approximately $15M per annum. Bovine genital campylobacteriosis is one of the major risk factors associated with this high cost due to the inability to accurately diagnose the disease. This research will integrate genomics and bioinformatics with infection and vaccination models to improve our understanding of the biology of the disease to develop appropriate control strategies securing Australia's beef industry.Read moreRead less