In vitro expression and crystallization of proteins from the thermohalophile, Halothermothrix orenii. The project proposal is to develop enabling state-of-the-art protein technologies for biotechnology. We will for the first time develop a rapid and high throughput technique for the expression, purification and crystallisation of proteins from the extreme thermohalophile, Halothermothrix orenii. This important break through in protein technology will assist in determining structure and function ....In vitro expression and crystallization of proteins from the thermohalophile, Halothermothrix orenii. The project proposal is to develop enabling state-of-the-art protein technologies for biotechnology. We will for the first time develop a rapid and high throughput technique for the expression, purification and crystallisation of proteins from the extreme thermohalophile, Halothermothrix orenii. This important break through in protein technology will assist in determining structure and function relationships of thermohalophilic proteins. It is expected that such an understanding together with comparative bioinformatics will assist researchers to develop "designer proteins" with improved functions for different uses in biotechnology. It is also expected that this technology will be amenable for use with other thermohalophilic microbes.Read moreRead less
Development of redox-mediated microbial assays for the rapid characterisation and assessment of wastewater, wastewater treatment processes and recycled water. With SE Qld on Level 5 water restrictions the need to reduce our demand on drinking water by increasing our reliance on recycled water is now urgent. To achieve this we need to ensure that the quality of our recycled wastewater is of a suitable and reliable standard. This project will assist the water industry in this endeavour by the de ....Development of redox-mediated microbial assays for the rapid characterisation and assessment of wastewater, wastewater treatment processes and recycled water. With SE Qld on Level 5 water restrictions the need to reduce our demand on drinking water by increasing our reliance on recycled water is now urgent. To achieve this we need to ensure that the quality of our recycled wastewater is of a suitable and reliable standard. This project will assist the water industry in this endeavour by the developing monitoring techniques that can rapidly characterise and assess the effectiveness of wastewater treatment processes and the quality of the water derived from them. These methods will provide early warnings of potential 'upsets' in treatment plants that would otherwise result in poor quality effluents that would need to be discharged as waste rather than be recycled.Read moreRead less
Investigation of Australian crop species for the rhizoremediation of residual sulfonyl urea herbicide contaminations in agricultural soils. This research aims to identify an environmentally sustainable and economically viable solution to the problem of residual herbicide contaminations in agricultural soils. The strategy is focused on stimulation of microbial degradation of pesticides in the root zone of crop species (Lupins). Such a strategy will improve crop yields and reduce soil contaminatio ....Investigation of Australian crop species for the rhizoremediation of residual sulfonyl urea herbicide contaminations in agricultural soils. This research aims to identify an environmentally sustainable and economically viable solution to the problem of residual herbicide contaminations in agricultural soils. The strategy is focused on stimulation of microbial degradation of pesticides in the root zone of crop species (Lupins). Such a strategy will improve crop yields and reduce soil contaminations and environmental impacts at minimal cost. Read moreRead less
Enhancing Seagrass Restoration : Improving Hessian Durability in Marine Environments. The establishment of an environmentally benign method for restoration of seagrass beds (which provide habitat for a wide range of commercially, recreationally and ecologically important marine species) will enhance Australia's capability of maintaining its coastal marine environment in support of its fishing and ecotourism industries. Furthermore, it will ultimately improve our capacity to manage the environmen ....Enhancing Seagrass Restoration : Improving Hessian Durability in Marine Environments. The establishment of an environmentally benign method for restoration of seagrass beds (which provide habitat for a wide range of commercially, recreationally and ecologically important marine species) will enhance Australia's capability of maintaining its coastal marine environment in support of its fishing and ecotourism industries. Furthermore, it will ultimately improve our capacity to manage the environmental impact of human development on our natural resources. If successful, the knowledge gained of the dynamics of marine biofilms on differentially coated natural fibers could facilitate broader application of methods used in environmental restoration and marine based industries, and foreshadow new applications.Read moreRead less
Ecosystem response to human, veterinary and growth promoting antibiotics in the aquatic environment. The objective of this study is to assess the threat to Australian aquatic ecosystems by antibiotics excreted and/or discarded through human activities each year. Specifically this study aims to: 1) Determine types of antibiotics, concentrations and distribution in surface waters downstream from potential sources; 2) Investigate bacterial resistance and bacterial community changes to antibiotic c ....Ecosystem response to human, veterinary and growth promoting antibiotics in the aquatic environment. The objective of this study is to assess the threat to Australian aquatic ecosystems by antibiotics excreted and/or discarded through human activities each year. Specifically this study aims to: 1) Determine types of antibiotics, concentrations and distribution in surface waters downstream from potential sources; 2) Investigate bacterial resistance and bacterial community changes to antibiotic compounds entering the aquatic environment; 3) Determine the influence of antibiotics on key ecological processes performed by bacteria - e.g. nitrogen cycle; 4) Assess effect on, and efficiency of, various effluent treatments in removing antibiotics and direct improvements for future removal.Read moreRead less
Microbial community characterisation for bioprocessing of chlorinated hydrocarbon contaminated groundwater. Due to irresponsible industrial practices, Australia has hundreds of polluted soil and water environments. This includes the notorious groundwater contamination underlying Botany in Sydney, an area of rich industrial, residential and cultural significance. The use of microbes to clean up polluted environments, such as the Botany groundwater, is known as bioremediation - a process exploitin ....Microbial community characterisation for bioprocessing of chlorinated hydrocarbon contaminated groundwater. Due to irresponsible industrial practices, Australia has hundreds of polluted soil and water environments. This includes the notorious groundwater contamination underlying Botany in Sydney, an area of rich industrial, residential and cultural significance. The use of microbes to clean up polluted environments, such as the Botany groundwater, is known as bioremediation - a process exploiting the natural metabolic versatility of microbes. To clean up the polluted groundwater in Botany, mixed species communities of pollutant degrading microbes are being produced. Novel tools to reveal the inner workings of these microscopic communities are also being developed, giving Australia an unprecedented advantage in the global bioremediation market.Read moreRead less
Novel Technology for Improving Disinfection Outcomes in Regional and Remote Drinking Water Distribution Systems. Climate shifts have led to water supply shortages in many areas of Australia, both in rural and remote regions and in cities. To ease water shortages, transportation of water over long distances is becoming increasingly necessary. A major impediment to transfer of water through long mains is management of the disinfectant: most disinfectants decay too rapidly, leaving consumers at ris ....Novel Technology for Improving Disinfection Outcomes in Regional and Remote Drinking Water Distribution Systems. Climate shifts have led to water supply shortages in many areas of Australia, both in rural and remote regions and in cities. To ease water shortages, transportation of water over long distances is becoming increasingly necessary. A major impediment to transfer of water through long mains is management of the disinfectant: most disinfectants decay too rapidly, leaving consumers at risk of pathogen exposure. Chloramination, the most viable disinfection technology for this purpose, suffers from a process called nitrification which accelerates disinfectant decay. In this project, we are developing an innovative, patented process to prevent nitrification, which will allow safe and effective disinfection of water supplies in long pipelines.Read moreRead less
Development of novel environmentally benign technologies for the control of bacterial biofilms in industrial applications. Bacteria will attach to and form biofilms on almost all surfaces. This is particularly a problem in moist environments, including food preparation surfaces, pipe networks (eg. water, oil, and gas), water purification systems. The effects of bacterial biofilms are wide ranging and impact on human health, our capacity to use water resources effectively, and the environment w ....Development of novel environmentally benign technologies for the control of bacterial biofilms in industrial applications. Bacteria will attach to and form biofilms on almost all surfaces. This is particularly a problem in moist environments, including food preparation surfaces, pipe networks (eg. water, oil, and gas), water purification systems. The effects of bacterial biofilms are wide ranging and impact on human health, our capacity to use water resources effectively, and the environment where toxic chemicals are normally used to kill the biofilm. The technologies under development here have the potential to reduce our reliance on toxic chemicals as well as contribute to significant reductions in the cost to purify and distribute vital resources such as water as well as reducing bacterial contamination food surfaces.Read moreRead less
Enhanced biocontrol options for the Australian sugar industry: a proteomic approach. Sugarcane is the second largest field crop grown in Australia and raw sugar is the third largest export product. Canegrubs are the major insect pest affecting production of sugarcane in Australia, currently costing the industry more than $12 million annually in insecticides and lost production. Biological control of insect pests provides an environmentally sound option of reducing these losses. Here we propos ....Enhanced biocontrol options for the Australian sugar industry: a proteomic approach. Sugarcane is the second largest field crop grown in Australia and raw sugar is the third largest export product. Canegrubs are the major insect pest affecting production of sugarcane in Australia, currently costing the industry more than $12 million annually in insecticides and lost production. Biological control of insect pests provides an environmentally sound option of reducing these losses. Here we propose to use proteomics to identify the key pathogenicity determinants in the biological control fungus Metarhizium during infection of canegrubs. The genes and proteins identified in this project will contribute to developing enhanced biocontrol options for Australian sugar industry.Read moreRead less
Environmental controls over Fe availability and transport in a forested coastal catchment. Iron is one of a number of elements that are mobilised in catchments, have recognised detrimental impacts to marine settings and are known to be nutrients for toxic cyanobacteria blooms. Knowledge of controls over distribution of Fe is important in catchment management and for sustainable forestry. The total catchment approach will result in an understanding of release and transport of Fe, and other heav ....Environmental controls over Fe availability and transport in a forested coastal catchment. Iron is one of a number of elements that are mobilised in catchments, have recognised detrimental impacts to marine settings and are known to be nutrients for toxic cyanobacteria blooms. Knowledge of controls over distribution of Fe is important in catchment management and for sustainable forestry. The total catchment approach will result in an understanding of release and transport of Fe, and other heavy metals. The outcomes of the study will enable useful comparison to coastal plantations elsewhere in Australia, and overseas. Determination of the relationship to rainfall and hydrological processes will enhance these comparisons plus consideration of climatic change.
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