Novel Molecular Markers for the Historical Source Tracing of Faecal Contamination in Urban Water Catchments. Protection of the microbiological quality of raw water systems is imperative to maintaining the safety of drinking water. Monitoring of water samples for the presence of microbes that indicate the presence of faecal pollution can be used to assess the possible threats to human health. The objective of this research is to apply molecular genetic methods to determine their effectiveness as ....Novel Molecular Markers for the Historical Source Tracing of Faecal Contamination in Urban Water Catchments. Protection of the microbiological quality of raw water systems is imperative to maintaining the safety of drinking water. Monitoring of water samples for the presence of microbes that indicate the presence of faecal pollution can be used to assess the possible threats to human health. The objective of this research is to apply molecular genetic methods to determine their effectiveness as tools for the tracking and tracing of faecal bacteria within drinking water catchments. We have chosen the spore-former Clostridium perfringens as an indicator of both long and short-term sewage contamination. It will enable us to predict the origin of contamination and thus identify potential sources of faecal pollution that require remediation.Read moreRead less
The biology, structure and function of bacterial virulence effectors. This project is closely aligned with the National Research Priority of Promoting and Maintaining Good Health and will establish a research framework to investigate novel virulence processes that allow bacterial pathogens to infect humans and cause disease. This fresh approach to the study of bacterial pathogenesis will sit outside classic genetic methods to investigate infection and immunity which rely heavily on genetic manip ....The biology, structure and function of bacterial virulence effectors. This project is closely aligned with the National Research Priority of Promoting and Maintaining Good Health and will establish a research framework to investigate novel virulence processes that allow bacterial pathogens to infect humans and cause disease. This fresh approach to the study of bacterial pathogenesis will sit outside classic genetic methods to investigate infection and immunity which rely heavily on genetic manipulation of the pathogen. Other than providing fundamental information on host-pathogen interactions, this work may lead to novel disease interventions by inhibition of bacterial virulence factor activity and/or enhancement of host inflammatory and immune responses.Read moreRead less
The development of a two-colour flow cytometric assay for the detection of whole cell biosensors in environmental samples. Macquarie University and the University of Copenhagen have expertise in fluorescence detection and whole cell biosensors respectively. The project will take advantage of these skills and develop a sensitive assay for monitoring biosensor bacteria in soil. The technology will be significant as it will enable real time analysis of antibiotic production in situ through the de ....The development of a two-colour flow cytometric assay for the detection of whole cell biosensors in environmental samples. Macquarie University and the University of Copenhagen have expertise in fluorescence detection and whole cell biosensors respectively. The project will take advantage of these skills and develop a sensitive assay for monitoring biosensor bacteria in soil. The technology will be significant as it will enable real time analysis of antibiotic production in situ through the detection of GFP expression. This work will then be used to isolate new antibiotic produces and will be extended to research into the bioavailability of toxic compounds and stress. An existing collaboration between the two institutions will be extended enabling the transfer and application of biosensor technology to Australia.Read moreRead less