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Improving clostridial toxoid production through molecular fermentation maps. This project aims to improve vaccine production by generating detailed molecular maps of fermentation which will be used to design superior fermentation processes with reduced cost. Toxoid vaccines, used routinely in the livestock industry to prevent animal-disease caused by pathogenic Clostridia, are produced using batch fermentation processes. These processes have undergone limited optimisation over the past five deca ....Improving clostridial toxoid production through molecular fermentation maps. This project aims to improve vaccine production by generating detailed molecular maps of fermentation which will be used to design superior fermentation processes with reduced cost. Toxoid vaccines, used routinely in the livestock industry to prevent animal-disease caused by pathogenic Clostridia, are produced using batch fermentation processes. These processes have undergone limited optimisation over the past five decades. Low titres and frequent batch failures greatly affect capital use and represent a significant cost. In addition, current optimisation approaches are limited by the use of expensive and noisy endpoint assays. This project aims to use high-throughput chemistry (multi-omics) that overcome these limitations.Read moreRead less
Synthetic phenazines for enhanced biogas production from renewable and non-renewable resources. Methane (biogas) has a large role to play in meeting the energy needs of the human race globally whilst reducing greenhouse gas emissions. Microbial communities are responsible for biogas production from non-renewable (coal) and renewable (food waste) resources. This project seeks to: increase biogas yields by redirecting electron flow towards biogas producing microbes using electrochemically active p ....Synthetic phenazines for enhanced biogas production from renewable and non-renewable resources. Methane (biogas) has a large role to play in meeting the energy needs of the human race globally whilst reducing greenhouse gas emissions. Microbial communities are responsible for biogas production from non-renewable (coal) and renewable (food waste) resources. This project seeks to: increase biogas yields by redirecting electron flow towards biogas producing microbes using electrochemically active phenazines; understand the molecular mechanism by which phenazines increase biogas yields; and, assess the environmental consequence of phenazine application to coal seam gas production and anaerobic digestion of food waste. Phenazines are likely to emerge as a safe and cost-effective technology for improved biogas generation.Read moreRead less
Energy from microalgae: industrial scale development and downstream processing of co-products. In this project Australian researchers will develop processes to produce renewable energy and commodity chemicals from microalgae grown in open saline ponds, enabling sustainable production of commercial quantities of clean biofuels and commodity products for the future. Renewable energy is an essential part of Australia's low greenhouse gas emissions energy target and is central to energy security. Th ....Energy from microalgae: industrial scale development and downstream processing of co-products. In this project Australian researchers will develop processes to produce renewable energy and commodity chemicals from microalgae grown in open saline ponds, enabling sustainable production of commercial quantities of clean biofuels and commodity products for the future. Renewable energy is an essential part of Australia's low greenhouse gas emissions energy target and is central to energy security. The development of microalgal energy will help Australia's determination to meet its Kyoto target. Additional benefits will include the development of value-added co-products from microalgal biomass, which is one route to commercial success of the novel technology.Read moreRead less
Developing an integrated systems and synthetic biology platform to expand the product spectrum of acetogens. This project aims to advance a waste gas fermentation process to enable the production of sustainable aviation fuel molecules for the first time. LanzaTech are world leaders in microbial gas fermentation and have produced ethanol at large scale in China. This project aims to combine the LanzaTech process with systems biology expertise at The University of Queensland to go beyond ethanol t ....Developing an integrated systems and synthetic biology platform to expand the product spectrum of acetogens. This project aims to advance a waste gas fermentation process to enable the production of sustainable aviation fuel molecules for the first time. LanzaTech are world leaders in microbial gas fermentation and have produced ethanol at large scale in China. This project aims to combine the LanzaTech process with systems biology expertise at The University of Queensland to go beyond ethanol to deliver new value-added products such as butanediol and farnesene. To achieve this aim the project will explore, understand and overcome fundamental energy and metabolic limitations in the production microorganism. Achieving the aims will be of direct relevance to SkyNRG and the new Brisbane Bioport on their path to deliver sustainable fuel to Brisbane Airport.Read moreRead less
Understanding bacteriophage deactivation and stabilisation in formulations. Bacteriophages (phages) are viruses that kill pathogenic bacteria without causing harms to the eco-balance. They can provide a safe and highly effective antimicrobial measure for biocontrol when formulated properly. This project aims to develop a mechanistic understanding of the physicochemical factors responsible for stabilising and deactivating phages in a wide range of formulations. It will create new knowledge on ke ....Understanding bacteriophage deactivation and stabilisation in formulations. Bacteriophages (phages) are viruses that kill pathogenic bacteria without causing harms to the eco-balance. They can provide a safe and highly effective antimicrobial measure for biocontrol when formulated properly. This project aims to develop a mechanistic understanding of the physicochemical factors responsible for stabilising and deactivating phages in a wide range of formulations. It will create new knowledge on key relationships between phage chemistry, phage-excipient interactions and phage stability. The research outcomes would significantly benefit Australia by enabling commercial development in the high value-adding area of environmentally friendly antimicrobial products.Read moreRead less
Sustainable dollar notes and other polypropylenes from bioderived feedstocks. Fossil fuels provide us with the essential chemicals for our life style. The chemical industry recognizes limited supply and a need to reduce carbon emissions. Microbes are able to supply green chemicals (e.g. bio-ethanol), but efficiencies are often low. This project will develop microbes for the fermentative production of plastics from cane sugar.
Unleashing the Hidden Chemical Diversity in Australian Fungi. This project aims to exploit an exclusive genomic resource consisting of >150 unique Australian filamentous fungi that has been built in a university-industry collaboration for genomic-guided biodiscovery. The genome sequence of these fungi revealed extensive hidden genetic instructions for production of novel biologically active molecules. The project will apply cutting-edge synthetic biology and chemical tools to tap into the hidden ....Unleashing the Hidden Chemical Diversity in Australian Fungi. This project aims to exploit an exclusive genomic resource consisting of >150 unique Australian filamentous fungi that has been built in a university-industry collaboration for genomic-guided biodiscovery. The genome sequence of these fungi revealed extensive hidden genetic instructions for production of novel biologically active molecules. The project will apply cutting-edge synthetic biology and chemical tools to tap into the hidden genomic potential of these Australian fungi. Expected outcomes of this project include new fine chemicals and lead molecules with desirable bioactivies. This will provide significant benefits to Australia's economy through the discovery of new pharmaceuticals, veterinary products and agrichemicals.Read moreRead less
Toward sustainable diesel production using microbial cells: unravelling isoprenoid pathway regulation through systems biology. The methylerythritol pyrophosphate (MEP) pathway for isoprenoid production is an essential biochemical pathway. It was only fully elucidated a decade ago, and the regulatory controls over this pathway are not understood. The objective of this project is to elucidate the mechanisms by which the MEP pathway is controlled in E. coli using a novel systems biology approach. U ....Toward sustainable diesel production using microbial cells: unravelling isoprenoid pathway regulation through systems biology. The methylerythritol pyrophosphate (MEP) pathway for isoprenoid production is an essential biochemical pathway. It was only fully elucidated a decade ago, and the regulatory controls over this pathway are not understood. The objective of this project is to elucidate the mechanisms by which the MEP pathway is controlled in E. coli using a novel systems biology approach. Understanding control of the MEP pathway is required to gain insight into homeostatic control of this essential pathway, and enable biotechnological engineering of E. coli in order to produce a wide range of industrially useful isoprenoids (including biofuels, pharmaceuticals, industrial chemicals, neutraceuticals, food additives, perfumes and many more).Read moreRead less
The use of in planta digestion for pretreatment of biofuel feedstock. This project will develop crop plants that overcome the current technical and economic impediments to the use of crop residues as biomass for large scale biofuel production. This innovation will position rural Australia at the forefront of global efforts to develop ligno-cellulose-based fuel ethanol industries and help meet mandatory renewable energy targets and the growing demand for alternative transport fuels. This project ....The use of in planta digestion for pretreatment of biofuel feedstock. This project will develop crop plants that overcome the current technical and economic impediments to the use of crop residues as biomass for large scale biofuel production. This innovation will position rural Australia at the forefront of global efforts to develop ligno-cellulose-based fuel ethanol industries and help meet mandatory renewable energy targets and the growing demand for alternative transport fuels. This project will also provide training and professional development for three early career researchers, exposing them to a suite of cutting edge technologies applied to a real world challenge - supplying renewable fuels in a sustainable and economically viable fashion.
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Using photosynthesis to power fine chemical production. This project aims to develop robust, solar driven, enzyme-based biocatalysts to synthesise complex metabolites and biopharmaceutical molecules in single-cell micro-algae. The significance of this project is that solar energy is used to drive challenging chemical reactions inexpensively via photosynthesis. Expected outcomes include sustainable, solar driven production of high-value molecules that are difficult or impossible to synthesise che ....Using photosynthesis to power fine chemical production. This project aims to develop robust, solar driven, enzyme-based biocatalysts to synthesise complex metabolites and biopharmaceutical molecules in single-cell micro-algae. The significance of this project is that solar energy is used to drive challenging chemical reactions inexpensively via photosynthesis. Expected outcomes include sustainable, solar driven production of high-value molecules that are difficult or impossible to synthesise chemically. Benefits include proof-of-principle demonstration of new, light-driven green-chemistry processes for the production of biochemicals, biopharmaceuticals, agricultural and industrial chemicals, extending the diversity of molecules we can make while reducing cost and environmental impact of their production.Read moreRead less