Discovery Early Career Researcher Award - Grant ID: DE120100723
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
The inheritance of epigenetic information in mammals. This project aims to understand how biological information can be passed from one generation to the next without being encoded in the genes. This may explain questions as diverse as why twins look subtly different and why some families are more likely than others to suffer disease.
Cell-type specific profiling of nascent RNA in the brain during learning. This project aims to understand cell-type specific, fast-acting, and dynamic patterns of RNA expression that arise during learning and contribute to the formation of memory. Activity-induced gene expression is central to neural plasticity, learning and memory. The project will apply a new approach, which tags RNA inside living cells. The findings will be broadly applicable and create new opportunities for understanding the ....Cell-type specific profiling of nascent RNA in the brain during learning. This project aims to understand cell-type specific, fast-acting, and dynamic patterns of RNA expression that arise during learning and contribute to the formation of memory. Activity-induced gene expression is central to neural plasticity, learning and memory. The project will apply a new approach, which tags RNA inside living cells. The findings will be broadly applicable and create new opportunities for understanding the true nature of brain adaptation.Read moreRead less
Understanding co-activator function in transcriptional regulation. A change in gene expression underpins all cell fate decisions yet there is scant knowledge about how transcription factors (TF), the master regulators of transcription, specifically interact with some, but not all, transcription cofactors to nuance gene expression. Aims: Using innovative molecular technologies we will identify and characterise the shared and unique relationships between TF and cofactors. Significance: This study ....Understanding co-activator function in transcriptional regulation. A change in gene expression underpins all cell fate decisions yet there is scant knowledge about how transcription factors (TF), the master regulators of transcription, specifically interact with some, but not all, transcription cofactors to nuance gene expression. Aims: Using innovative molecular technologies we will identify and characterise the shared and unique relationships between TF and cofactors. Significance: This study is important to every biological process in plants and animals driven by a change in gene expression. Expected Outcomes: This study will increase our biological knowledge in transcription control. Benefit: The knowledge gained has future applications in genomics and broad implications for biotechnology and industry.Read moreRead less
Multi-trait plasticity in response to a changing climate. This project aims to understand the effect of climate change on natural populations. Phenotypic plasticity (the ability to change phenotype with environment) determines natural populations’ immediate response to environmental change. However, studies of plasticity frequently rely on simplifying assumptions, and understanding the genomic and epigenomic mechanisms underlying plasticity is only just emerging. This project will combine a fine ....Multi-trait plasticity in response to a changing climate. This project aims to understand the effect of climate change on natural populations. Phenotypic plasticity (the ability to change phenotype with environment) determines natural populations’ immediate response to environmental change. However, studies of plasticity frequently rely on simplifying assumptions, and understanding the genomic and epigenomic mechanisms underlying plasticity is only just emerging. This project will combine a fine-scale temperature-manipulation experiment with genomic and multivariate statistical analyses of a native Australian alpine plant. The intended outcome is a comprehensive analysis of whether multi-trait phenotypic plasticity is adaptive; whether it can evolve; and the epigenomic mechanisms that drive it. The project will predict the likely effect of temperature change on alpine plants, and so generate information internationally relevant to the management of populations adapting to climate change and locally relevant to the conservation of Australian montane flora.Read moreRead less
Characterising inheritance patterns of whole genome DNA methylation. This project aims to characterise epigenetic diversity and inheritance patterns in whole genome sequencing data from a unique human population. The project will employ the well-characterised Norfolk Island genetic isolate, cost-effective whole genome bisulphite sequencing technologies and advanced bioinformatics pipelines and statistical models. It will involve cross-discipline collaboration between human geneticists, epigeneti ....Characterising inheritance patterns of whole genome DNA methylation. This project aims to characterise epigenetic diversity and inheritance patterns in whole genome sequencing data from a unique human population. The project will employ the well-characterised Norfolk Island genetic isolate, cost-effective whole genome bisulphite sequencing technologies and advanced bioinformatics pipelines and statistical models. It will involve cross-discipline collaboration between human geneticists, epigeneticists, statistical geneticists and bioinformaticians. This project will advance our understanding of the interaction of genetics and epigenetics and their relationship to diversity and inheritance in humans.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE180100624
Funder
Australian Research Council
Funding Amount
$365,058.00
Summary
The role of epigenetic modifications in tiger snake adaptation. This project aims to investigate mechanisms underlying animal adaptation to future environmental change by examining the molecular basis for phenotypic plasticity in snakes. This project will specifically examine variation in genetic/epigenetic profiles and compare against important fitness traits, such as variable head size, bite force and swallowing performance to identify relationships between molecular change and physiology. Suc ....The role of epigenetic modifications in tiger snake adaptation. This project aims to investigate mechanisms underlying animal adaptation to future environmental change by examining the molecular basis for phenotypic plasticity in snakes. This project will specifically examine variation in genetic/epigenetic profiles and compare against important fitness traits, such as variable head size, bite force and swallowing performance to identify relationships between molecular change and physiology. Such research is a critical first step in improving our knowledge of the mechanisms whereby animal populations may adapt to environmental change, allowing us to facilitate such processes or concentrate conservation effort where species are unable to adapt via epigenetic modification.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150101206
Funder
Australian Research Council
Funding Amount
$372,536.00
Summary
Beyond genes: How the extended genotype of plants facilitates adaptation. Adaptation to environmental change is required for species to persist, however rapid environmental change may exceed the limits of traditional genetic adaptation leading to widespread decline. Recent work has highlighted the 'extended genotype' as an additional factor influencing adaptive phenotypes. This project aims to examine DNA methylation and polyploidisation as both a cause and consequence of the adaptation process ....Beyond genes: How the extended genotype of plants facilitates adaptation. Adaptation to environmental change is required for species to persist, however rapid environmental change may exceed the limits of traditional genetic adaptation leading to widespread decline. Recent work has highlighted the 'extended genotype' as an additional factor influencing adaptive phenotypes. This project aims to examine DNA methylation and polyploidisation as both a cause and consequence of the adaptation process using natural populations of the model cereal Brachypodium distachyon. The project aims to determine the architecture of these features and how their variability impacts adaptive traits such as flowering time. From the functional role of the extended genotype the project endeavours to predict and select genetic responses to the environment.Read moreRead less
Epigenetics, environment, and evolution. This project will aim to understand how biological information can exist and be passed from one generation to the next without being encoded in the gene sequence, and also how our early environment can modify this so-called "epigenetic" information to alter disease risk.
Intron splicing regulates gene silencing in Arabidopsis. Defective gene regulation (i.e. how genes switch on and off) can cause severe genetic disease in both plants and animals, including humans. This project will use plants as a model to investigate a cause of defective gene expression, and should reveal possible avenues for therapeutic intervention to correct genetic defects in plants and animals.
Next-generation epigenetic analysis: direct reading of DNA methylation. This project aims to develop a new molecular tool to directly and dynamically read chemical modifications on genomic DNA (epigenetics) by utilizing advanced nanomaterials with the unique features of Raman spectroscopy. Epigenetics affects cellular processes and controls genetic programs by turning them “on” and “off" but there is currently no direct method to measure modifications on DNA. A new technology will be designed to ....Next-generation epigenetic analysis: direct reading of DNA methylation. This project aims to develop a new molecular tool to directly and dynamically read chemical modifications on genomic DNA (epigenetics) by utilizing advanced nanomaterials with the unique features of Raman spectroscopy. Epigenetics affects cellular processes and controls genetic programs by turning them “on” and “off" but there is currently no direct method to measure modifications on DNA. A new technology will be designed to avoid complicated procedures/chemistry for DNA epigenetic analysis providing a specific molecular fingerprint. The anticipated outcomes include a new technique and advanced knowledge in nanomaterials and DNA functions, thus strengthening the economic viability of Australian manufacturing and biotechnology sectors.Read moreRead less