Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100181
Funder
Australian Research Council
Funding Amount
$650,000.00
Summary
Strengthening merit-based access and support at the new National Computing Infrastructure petascale supercomputing facility. World-leading high-performance computing is fundamental to Australia's international research success. This facility will provide access to the new National Computational Infrastructure facility by world-leading researchers from six research universities, and sustain ground-breaking work in an increasingly competitive environment.
Understanding the structural basis for catalysis and substrate specificity in non-heme diiron medium-chain alkane hydroxylases. This work will determine the molecular basis for catalysis and specificity of non-heme diiron medium-chain alkane hydroxylases by obtaining the first structure of such an enzyme. This pivotal knowledge will drive enzyme engineering for applications in biodegradation of spilt oils and biosynthesis of pharmaceuticals and high-cost chemicals.
Biosynthetic LEGO: enzymatic redesign to produce new vancomycin analogues. This project aims to uncover the reengineering potential of the biosynthetic machinery that produces glycopeptide antibiotics by advancing our understanding of how the core peptide production line functions. Natural product biosynthesis often produces complex peptide structures, with one important example being the glycopeptide antibiotics. This project expects to generate new knowledge about enzymatic peptide biosynthesi ....Biosynthetic LEGO: enzymatic redesign to produce new vancomycin analogues. This project aims to uncover the reengineering potential of the biosynthetic machinery that produces glycopeptide antibiotics by advancing our understanding of how the core peptide production line functions. Natural product biosynthesis often produces complex peptide structures, with one important example being the glycopeptide antibiotics. This project expects to generate new knowledge about enzymatic peptide biosynthesis using a highly interdisciplinary approach and previously developed tools. The anticipated outcomes of this project will be an enhanced understanding of how such complex peptide biosynthesis is performed, which is knowledge vital for future efforts to reengineer such biosynthetic peptide assembly lines as a series of modular LEGO blocks to produce new bioactive peptides.Read moreRead less
Deciphering Electron Transfer Pathways in Bacteria. Enzyme catalysed oxidation reactions are key players in the production of naturally occurring biologically active molecules. These processes are tightly regulated by their electron transfer partners. This project aims to characterise new electron transfer ferredoxin proteins from a metabolically diverse bacterium. These ferredoxins, important in many bacteria, contain different non-cysteine amino acids in their iron-sulfur cluster binding motif ....Deciphering Electron Transfer Pathways in Bacteria. Enzyme catalysed oxidation reactions are key players in the production of naturally occurring biologically active molecules. These processes are tightly regulated by their electron transfer partners. This project aims to characterise new electron transfer ferredoxin proteins from a metabolically diverse bacterium. These ferredoxins, important in many bacteria, contain different non-cysteine amino acids in their iron-sulfur cluster binding motifs and are poorly defined. The outcomes will advance understandings of electron transfer, a fundamental process. This will allow strategies to combat human and plant pathogens and unlock the potential of these systems as biocatalysts for the green chemical synthesis of complex and valuable chemicals.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE110100078
Funder
Australian Research Council
Funding Amount
$500,000.00
Summary
Establishment of a comprehensive regional biophysical analysis facility. Interactions between molecules are needed for cells to function correctly. This facility will permit comprehensive molecular characterisation as well as research into the fundamentals of how molecules interact.
Short circuiting redox enzymes. Enzymes that catalyse oxidation or reduction reactions can be integrated with an electrode in the development of biosensors. A key challenge is enabling an electrical current between the enzyme and the electrode and this project aims to probe this phenomenon to provide an enzyme system that operates with greater efficiency than in nature.
Visualising chaperones disentangle and refold proteins - one molecule at a time. Chaperones are enzymes that maintain the proper function of proteins in the cell. This research aims to visualise, at the single molecule level, how chaperones facilitate the folding of individual proteins and how they can disentangle proteins that have aggregated as a result of cell stress.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100224
Funder
Australian Research Council
Funding Amount
$250,000.00
Summary
Multi-mode fluorescence microscope for visualising the dynamics of cellular processes at the single-molecule level. Fluorescence is the emission of light by a substance that has absorbed light of a different wavelength. This fluorescence microscopy facility will allow the visualisation of the dynamic processes that define life at the molecular level. This insight will help us understand cellular function and how it is impaired in various diseases including cancer and neurodegenerative disorders ....Multi-mode fluorescence microscope for visualising the dynamics of cellular processes at the single-molecule level. Fluorescence is the emission of light by a substance that has absorbed light of a different wavelength. This fluorescence microscopy facility will allow the visualisation of the dynamic processes that define life at the molecular level. This insight will help us understand cellular function and how it is impaired in various diseases including cancer and neurodegenerative disorders such as Parkinson’s and Alzheimer’s disease.Read moreRead less
A microbiological platform for the production of complex small molecules with important biological activities. Currently making complex chemicals such as medicines relies on harvesting rare natural resources or using complicated, energy intensive laboratory-based processes. This project will overcome these limitations by using a natural biological system to produce these chemicals, returning significant health and economic benefits to the nation.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100127
Funder
Australian Research Council
Funding Amount
$355,000.00
Summary
Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein funct ....Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein function inside living cells. The new equipment is designed to provide a fast super-resolution imaging system to study the intracellular dynamics of proteins in vitro and a super-resolution microscope to visualise structures and assemblies inside microbes with a resolution of tens of nanometres, putting in vitro biochemistry into the context of a living cell. Read moreRead less