In-line SPE-CE for the direct determination of drugs and metabolites in biological fluids based on porous polymer monoliths. A new approach is proposed for the development of novel micro- and nano-scale solid-phase adsorbent materials. These materials are based on porous polymer monoliths formed in situ within a capillary acting as a mould. The allows the material to be readily placed in a defined position, in a process similar to photo-patterning, and alleviates many of the problems usually as ....In-line SPE-CE for the direct determination of drugs and metabolites in biological fluids based on porous polymer monoliths. A new approach is proposed for the development of novel micro- and nano-scale solid-phase adsorbent materials. These materials are based on porous polymer monoliths formed in situ within a capillary acting as a mould. The allows the material to be readily placed in a defined position, in a process similar to photo-patterning, and alleviates many of the problems usually associated with fabrication and miniaturisation. A simple photo-grafting process, initiated by UV light can be used for the selective chemical modification of these materials. These tailored monoliths can then be used for the in-line coupling of biological sample handling and capillary electrophoresis (CE) for the determination of drugs and related metabolites in biological fluids, thus avoiding time-consuming and costly off-line sample pre-treatment. This will lead to the development of new methods for the rapid determination of drugs, metabolites and other small molecules in clinical samples.Read moreRead less
Uncovering the genetic basis for saxitoxin production in Australian marine and freshwater systems: novel molecular tools for management. In Australia, toxic algal blooms have had a devastating impact on marine and freshwater resources. In collaboration with a biotechnology company, this project will use an innovative method to design a molecular genetic tool to monitor, research and potentially mitigate the effects of saxitoxin production on water supplies and aquaculture industries. In working ....Uncovering the genetic basis for saxitoxin production in Australian marine and freshwater systems: novel molecular tools for management. In Australia, toxic algal blooms have had a devastating impact on marine and freshwater resources. In collaboration with a biotechnology company, this project will use an innovative method to design a molecular genetic tool to monitor, research and potentially mitigate the effects of saxitoxin production on water supplies and aquaculture industries. In working with monitoring authorities throughout Australia, we will produce a specific, sensitive and cost-effective technology that will ultimately be applicable worldwide. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0452281
Funder
Australian Research Council
Funding Amount
$102,900.00
Summary
Muscle Vascular Research and Gene Therapy Using Ultrasound. We seek funds to set up a national facility for ultrasound in muscle vascular research and gene therapy. Ultrasound with microbubbles will be used for the imaging of muscle microvascular blood flow and as a delivery modality for gene constructs to endothelial cells that control blood flow. The technology has application to (i) the assessment and therapeutic treatment of impaired microvascular function as in diabetics; (ii) the assessmen ....Muscle Vascular Research and Gene Therapy Using Ultrasound. We seek funds to set up a national facility for ultrasound in muscle vascular research and gene therapy. Ultrasound with microbubbles will be used for the imaging of muscle microvascular blood flow and as a delivery modality for gene constructs to endothelial cells that control blood flow. The technology has application to (i) the assessment and therapeutic treatment of impaired microvascular function as in diabetics; (ii) the assessment of adaptation to physical training and (iii) the development of therapeutic agents used to treat diabetes. We anticipate improved technology that is fully characterized and novel therapeutics that improve microvascular blood flow.Read moreRead less