Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0882890
Funder
Australian Research Council
Funding Amount
$130,000.00
Summary
Investigating protein/protein interactions. To establish and maintain a prominent position in scientific research, Australian scientists must have access to state of the art technology. The Bio21 Institute is a multidisciplinary research centre specialising in medical agricultural and environmental science and is ideally suited to house an SPR facility. SPR provides unique functional, kinetic and thermodynamic information on molecular interactions which give rise to both physiological and path ....Investigating protein/protein interactions. To establish and maintain a prominent position in scientific research, Australian scientists must have access to state of the art technology. The Bio21 Institute is a multidisciplinary research centre specialising in medical agricultural and environmental science and is ideally suited to house an SPR facility. SPR provides unique functional, kinetic and thermodynamic information on molecular interactions which give rise to both physiological and pathological outcomes. A detailed knowledge of molecular interactions is fundamental to the understanding of all biological systems. When placed at the Bio21 Institute, the facility will foster the development of diversified collaborations between the applicants and the wider research community.Read moreRead less
High throughput engineering of genetically encodable fluorescent sensors of intracellular signalling networks. Understanding of biochemical processes in living organisms is central to biological research and drug discovery. At present, the field suffers from a chronic paucity of adequate observation methods. The proposed project represents an interdisciplinary effort to create approaches for real-time monitoring of complex cellular chemistries. This work will deliver novel technologies for use i ....High throughput engineering of genetically encodable fluorescent sensors of intracellular signalling networks. Understanding of biochemical processes in living organisms is central to biological research and drug discovery. At present, the field suffers from a chronic paucity of adequate observation methods. The proposed project represents an interdisciplinary effort to create approaches for real-time monitoring of complex cellular chemistries. This work will deliver novel technologies for use in diagnostics and drug development. It will provide vital information on the changes in cellular processes induced by malignant transformation, viral infection and aging. This work will generate both health and economic benefits for the community and have a positive impact on the international visibility of Australian biomedical research.Read moreRead less
Correlation between surface force and morphology of self-assembled monolayer. As a most potential solid support for biological molecules without denaturing their functions, gold thiol self-assembled monolayers (SAMs) have been studied extensively for surface fabrication. In this project we will prepare functional monolayers using newly synthesized thiol derivatives, evaluate correlation between surface force and surface morphology of the SAMs using the surface forces apparatus, and optimize the ....Correlation between surface force and morphology of self-assembled monolayer. As a most potential solid support for biological molecules without denaturing their functions, gold thiol self-assembled monolayers (SAMs) have been studied extensively for surface fabrication. In this project we will prepare functional monolayers using newly synthesized thiol derivatives, evaluate correlation between surface force and surface morphology of the SAMs using the surface forces apparatus, and optimize the condition to fabricate biological devices such as immune sensors.Read moreRead less
Liquid light: aqueous bio-sensing in microstructured polymer optical fibres. This project builds on Australia's world-leading position in the development of microstructured polymer optical fibres, and applies the unique benefits they provide to for ultra- sensitive bio-sensing. By using the microstructure to simultaneously confine light and liquid, microstructured optical fibres provide a unique platform for ultra-sensitive spectroscopy and structural studies of biomolecules in solution. The wor ....Liquid light: aqueous bio-sensing in microstructured polymer optical fibres. This project builds on Australia's world-leading position in the development of microstructured polymer optical fibres, and applies the unique benefits they provide to for ultra- sensitive bio-sensing. By using the microstructure to simultaneously confine light and liquid, microstructured optical fibres provide a unique platform for ultra-sensitive spectroscopy and structural studies of biomolecules in solution. The work has profound implications both for fundamental science and applications, particularly in medical diagnostics.Read moreRead less
Probing the function of protein molecular motors on nano-fabricated structures. The function of protein linear molecular motors, which are natural dynamic bio-nano-devices with a ubiquitous importance in multicellular organisms, will be 'probed' with purposefully designed nano-structures fabricated via photo- or Scanning Probe Microscopy Lithography, that is, flat polymeric surfaces with combinatorial combinations of physico-chemistries; and micro/nano-channels and nano-wells with critical dimen ....Probing the function of protein molecular motors on nano-fabricated structures. The function of protein linear molecular motors, which are natural dynamic bio-nano-devices with a ubiquitous importance in multicellular organisms, will be 'probed' with purposefully designed nano-structures fabricated via photo- or Scanning Probe Microscopy Lithography, that is, flat polymeric surfaces with combinatorial combinations of physico-chemistries; and micro/nano-channels and nano-wells with critical dimensions similar to the scale of the probed biomolecules. The project turns 'up-side down' the challenge of invasive nano-probing of biomolecules using it in an engineered manner. The fundamental understanding of linear molecular motors will impact on biomedical science and on the assessment of hybrid natural-artificial dynamic nano-devices.Read moreRead less
New Membrane Chips For Protein Interaction Analysis. This proposal is based on a strategic partnership between Monash University and Farfield Sensors. We will create a series of new biosensors that will be used to establish a new approach to the structural analysis of membrane protein function. In particular, this technology may lead to the identification of new proteins and drug targets for therapeutic development. The long-term outcome would be the development of improved therapeutics which wo ....New Membrane Chips For Protein Interaction Analysis. This proposal is based on a strategic partnership between Monash University and Farfield Sensors. We will create a series of new biosensors that will be used to establish a new approach to the structural analysis of membrane protein function. In particular, this technology may lead to the identification of new proteins and drug targets for therapeutic development. The long-term outcome would be the development of improved therapeutics which would be coupled to potential economic returns when further commercialisation is achieved. Read moreRead less