Biomolecular activity modulated by interaction with nanostructures. Nanotechnological methods are able to reliably fabricate artificial nanostructures with dimensions similar to those of large biomolecules (a few to tens of nanometers). This study focuses on the interaction of artificial nanostructures with biomolecules such as proteins and DNA, and will enable scientists to better understand biomolecular recognition and binding events, which are central to all biological processes. The underst ....Biomolecular activity modulated by interaction with nanostructures. Nanotechnological methods are able to reliably fabricate artificial nanostructures with dimensions similar to those of large biomolecules (a few to tens of nanometers). This study focuses on the interaction of artificial nanostructures with biomolecules such as proteins and DNA, and will enable scientists to better understand biomolecular recognition and binding events, which are central to all biological processes. The understanding gained can then be used to design biomimetic surfaces for use in health monitoring and medical diagnostic devices with improved sensitivity, robustness and portability, thereby providing significant benefits to the health sector.Read moreRead less
Electric field induced surface attachment and detachment of proteins. Microarrays are revolutionising the diagnosis of disease by enabling large amounts of data on genetics and protein expression to be obtained from one sample. Biosensors for diseases and toxins rely on the same mechanism, namely attachment of biological macromolecules to a surface. We propose a new method for controlling the attachment by micromachining an electrode system to apply an electric field to chosen sites. Ultimately ....Electric field induced surface attachment and detachment of proteins. Microarrays are revolutionising the diagnosis of disease by enabling large amounts of data on genetics and protein expression to be obtained from one sample. Biosensors for diseases and toxins rely on the same mechanism, namely attachment of biological macromolecules to a surface. We propose a new method for controlling the attachment by micromachining an electrode system to apply an electric field to chosen sites. Ultimately microelectronic engineering methods will be used. This will give control over the attachment process with potential benefits of orienting attaching molecules, minimising non-specific attachment and enriching diagnostics by enabling interrogation of the force of attachment.Read moreRead less
Bacterial cell behaviour in micro/nano-confined environments. The project aims to progress the understanding of the mechanisms of marine bacteria biofilm formation and surface-modulated metabolic response. We will adjust the surface characteristics of photopolymers (as ?model? surfaces) and probe the bacterial response to surfaces, passively with micro/nano-fabricated structures and Atomic Force Microscopy; and actively with optical manipulation of single cells. The results will contribute to th ....Bacterial cell behaviour in micro/nano-confined environments. The project aims to progress the understanding of the mechanisms of marine bacteria biofilm formation and surface-modulated metabolic response. We will adjust the surface characteristics of photopolymers (as ?model? surfaces) and probe the bacterial response to surfaces, passively with micro/nano-fabricated structures and Atomic Force Microscopy; and actively with optical manipulation of single cells. The results will contribute to the fundamental knowledge regarding central biological phenomena -down to single-cell processes- as well as on applied knowledge regarding the manufacturing of antimicrobial surfaces that mimic natural bactericide processes, with larger implications on biomedical practice, and environmental, civil, mining and manufacturing industrial applications.Read moreRead less