Cell–fluid interaction: inside and outside cells. The project aims to measure mechanics at the cellular level using a combination of optical tweezers for measurement of nano-scale environment around/inside cells and light-sheet microscopy for imaging. The project expects to generate new knowledge about movement of cells through their environment, relating to collective behaviour which is of importance in understanding infections and formation of biofilms. Expected outcomes include deepened under ....Cell–fluid interaction: inside and outside cells. The project aims to measure mechanics at the cellular level using a combination of optical tweezers for measurement of nano-scale environment around/inside cells and light-sheet microscopy for imaging. The project expects to generate new knowledge about movement of cells through their environment, relating to collective behaviour which is of importance in understanding infections and formation of biofilms. Expected outcomes include deepened understanding of an enigmatic process conserved from amoebae to humans, by which cells ‘drink and eat’ by ‘gulping’ fluid and supplement their nutrient intake by degrading proteins and cell debris. It will generate new knowledge of these processes to better understand how mechanics affects cellular life.Read moreRead less
Biophysics of the brain’s waste disposal system: Understanding why we sleep. This project aims to develop a new biophysical model of the brain, founded on the recently discovered glymphatic system responsible for waste disposal during sleep. It sets out to formulate, analyse, and validate rigorous new multiscale quantitative modelling – to advance the study of sleep and brain clearance dynamics, at timescales from hours to decades. Among expected outcomes are powerful models ready for applicatio ....Biophysics of the brain’s waste disposal system: Understanding why we sleep. This project aims to develop a new biophysical model of the brain, founded on the recently discovered glymphatic system responsible for waste disposal during sleep. It sets out to formulate, analyse, and validate rigorous new multiscale quantitative modelling – to advance the study of sleep and brain clearance dynamics, at timescales from hours to decades. Among expected outcomes are powerful models ready for application at both population and individual level, and testable predictions concerning the sleep patterns that lead to aggregation of waste in the brain and eventual cognitive decline. Project outcomes should also benefit society and the economy though translation into interventions for sleep disturbance – in future applied research.Read moreRead less
The geometry of genome access: lessons from HIV. Access to the cell’s nucleus, and hence its genome, is of deep scientific and commercial significance. It is controlled by a phase-separated diffusion barrier within the nuclear pore complex. Recent evidence, however, has shown that HIV can cross this barrier with its protective capsid intact, despite it being over one thousand times larger than the limit for passive transport. Combining concepts from soft-matter physics with recombinant assays, t ....The geometry of genome access: lessons from HIV. Access to the cell’s nucleus, and hence its genome, is of deep scientific and commercial significance. It is controlled by a phase-separated diffusion barrier within the nuclear pore complex. Recent evidence, however, has shown that HIV can cross this barrier with its protective capsid intact, despite it being over one thousand times larger than the limit for passive transport. Combining concepts from soft-matter physics with recombinant assays, this project aims to uncover the link between the unique geometry of HIV capsids and their ability to subvert the nucleus’ defenses. The expected outcome is a step-change in the understanding of nuclear access control, with downstream benefits to virology, bio-engineering and bio-technology.Read moreRead less
Untangling the matrix of bacterial biofilms. This research aims to use forefront molecular microbiology and biophysical approaches to advance fundamental knowledge on bacterial biofilms. These bacterial clusters are held together by an extracellular matrix comprised of bacterial-derived fibrous protein and the polysaccharide cellulose, which imparts structural integrity and resistance to antimicrobials. The major goals of this project are to dissect how bacteria regulate production of the biofil ....Untangling the matrix of bacterial biofilms. This research aims to use forefront molecular microbiology and biophysical approaches to advance fundamental knowledge on bacterial biofilms. These bacterial clusters are held together by an extracellular matrix comprised of bacterial-derived fibrous protein and the polysaccharide cellulose, which imparts structural integrity and resistance to antimicrobials. The major goals of this project are to dissect how bacteria regulate production of the biofilm matrix, and examine how changes in the composition of the matrix alters its properties, including the penetration of antimicrobial peptides and antibiotics. The outcomes will help address the economic burden of difficult to treat industrial, environmental and biomedical biofilms.Read moreRead less
Reading the sequence of a single molecule of DNA . This project seeks to develop technology capable of accurately reading the sequence of a single DNA molecule for the first time. This is possible by combining state-of-the-art methods in DNA self-assembly, single-molecule fluorescence microscopy and bioelectronics, to overcome fundamental limits in current technologies.
The outcome of accurate DNA sequencing at single molecule resolution, promises ground-breaking biological insight from a more ....Reading the sequence of a single molecule of DNA . This project seeks to develop technology capable of accurately reading the sequence of a single DNA molecule for the first time. This is possible by combining state-of-the-art methods in DNA self-assembly, single-molecule fluorescence microscopy and bioelectronics, to overcome fundamental limits in current technologies.
The outcome of accurate DNA sequencing at single molecule resolution, promises ground-breaking biological insight from a more fine-grained view of the genetic world, game-changing technologies such as point-of-care genomics and in turn a substantial impact on the rapidly growing multi-billion-dollar DNA sequencing market. Read moreRead less
Comparing properties of innate immune proteins of bats and humans. Supra-molecular protein complexes known as signalosomes drive our innate immune response by forming large signaling hubs capable of recruiting downstream effectors. This project aims to compare the properties and structure of human and bat signalosomes and discover the molecular origins of the “supra-immunity” of bats. In this context, the project expects to generate new knowledge concerning the fundamental molecular mechanisms t ....Comparing properties of innate immune proteins of bats and humans. Supra-molecular protein complexes known as signalosomes drive our innate immune response by forming large signaling hubs capable of recruiting downstream effectors. This project aims to compare the properties and structure of human and bat signalosomes and discover the molecular origins of the “supra-immunity” of bats. In this context, the project expects to generate new knowledge concerning the fundamental molecular mechanisms that regulate the signalosomes. The intended outcome is to answer the long-standing question of control of speed and amplitude of innate immune response at the molecular level. Both locally and internationally, this new approach should provide benefits across structural biology, molecular evolution and biotechnology.Read moreRead less
Nature’s advanced optical materials and their role in thermal management. This project aims to discover the nano-structural properties of beetles than enable effective management of solar and thermal radiation in different environments. A further aim is to reveal how these composite biological materials combine thermal control with desirable mechanical properties, such as strength and flexibility. Passive control of radiative energy is critical for both animal survival and for the design of many ....Nature’s advanced optical materials and their role in thermal management. This project aims to discover the nano-structural properties of beetles than enable effective management of solar and thermal radiation in different environments. A further aim is to reveal how these composite biological materials combine thermal control with desirable mechanical properties, such as strength and flexibility. Passive control of radiative energy is critical for both animal survival and for the design of many manufactured materials, particularly in a warming world. This interdisciplinary project will provide new knowledge of the different ways that biological materials mediate radiative energy exchange with the environment. This knowledge is essential for the design of bioinspired, energy-efficient, multi-functional materials.Read moreRead less