5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids. Rapid and accurate quantification of live biological fluid properties at sub-cellular and molecular levels forms the bedrock of biofluidic sciences. Majority of the biofluidic devices rely on quantifying biological fluids after its removal from the body in an in vitro Flow Cytometer (FC). FC faces many caveats i.e. biological degradation and small volume etc. In this project, we shall engineer the first in vivo 5D imaging ....5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids. Rapid and accurate quantification of live biological fluid properties at sub-cellular and molecular levels forms the bedrock of biofluidic sciences. Majority of the biofluidic devices rely on quantifying biological fluids after its removal from the body in an in vitro Flow Cytometer (FC). FC faces many caveats i.e. biological degradation and small volume etc. In this project, we shall engineer the first in vivo 5D imaging flow cytometer (5D IFC) capable of continuous assessment of potentially entire blood volume in a living mice without removing fluid out of the body. The project represents a major advancement beyond any existing flow cytometer and overcome the engineering limits of state-of-art laser scanning imaging devices.Read moreRead less
Molecular mechanisms of mechanosensation and shape regulation in cells. This project aims to explore how cells physically sense and respond to the surrounding environment on a molecular level. Physical distortion of erythrocytes doubles their glucose consumption and increases cation membrane flux five-fold. This mechanism involves opening of the mechanosenstive ion channel Piezo1. This project will include a kinetic description of these phenomena, with a goal to establish a predictive mathematic ....Molecular mechanisms of mechanosensation and shape regulation in cells. This project aims to explore how cells physically sense and respond to the surrounding environment on a molecular level. Physical distortion of erythrocytes doubles their glucose consumption and increases cation membrane flux five-fold. This mechanism involves opening of the mechanosenstive ion channel Piezo1. This project will include a kinetic description of these phenomena, with a goal to establish a predictive mathematical model of the regulation of cell-shape and volume. The project will provide an understanding of mechanisms operating when cells and tissues are succumbing to trauma and invasion, and how to control these processes on a molecular level.Read moreRead less