Biotechnology applications in the veterinary pharmaceutical industry: Pharmacological manipulation of ovarian function in domestic animals. The purpose of this research is to develop a humane, simple, safe, effective means of sterilizing female domestic animals without the need for surgical intervention. This novel experimental strategy is to target the supply of dormant eggs in the ovaries of female mammals. Every female mammal is born with a finite supply of eggs in the ovarian cortex. These c ....Biotechnology applications in the veterinary pharmaceutical industry: Pharmacological manipulation of ovarian function in domestic animals. The purpose of this research is to develop a humane, simple, safe, effective means of sterilizing female domestic animals without the need for surgical intervention. This novel experimental strategy is to target the supply of dormant eggs in the ovaries of female mammals. Every female mammal is born with a finite supply of eggs in the ovarian cortex. These cells cannot divide and so when this store is exhausted, reproduction ceases. Our strategy is to develop pharmaceutical reagents that will destroy this dormant egg population, thereby rendering the animals instantaneously and irreversibly infertile.Read moreRead less
Sex preselection of stallion spermatozoa. This study will develop novel techniques that will maintain and optimise the fertility of sex-sorted stallion semen before and after processing through a flow cytometer. Ultimately, spermatozoa obtained from ejaculation or from the testes sperm storage site (epididymes) at the time of castration will be sorted into X- (female) or Y- (male) populations and used to inseminate mares and thus, enable horse breeders to predetermine the sex of their foal at th ....Sex preselection of stallion spermatozoa. This study will develop novel techniques that will maintain and optimise the fertility of sex-sorted stallion semen before and after processing through a flow cytometer. Ultimately, spermatozoa obtained from ejaculation or from the testes sperm storage site (epididymes) at the time of castration will be sorted into X- (female) or Y- (male) populations and used to inseminate mares and thus, enable horse breeders to predetermine the sex of their foal at the time of insemination. The introduction of sex-sorted semen into the Australian horse industry (worth some $6.2 billion) will ensure that Australia leads the world in equine reproductive technology.Read moreRead less
Enhancing the efficiency of equine reproduction: relevant to the Thoroughbred and Standardbred breeding industries. The purpose of this project is to link the equine breeding industry with a major centre of reproductive research at the University of Newcastle. By creating this unique nexus, major efficiency gains will be achieved for this industry that will not only secure its international competitiveness but also significantly enhance its profitability.
Enhancing fertility for the Thoroughbred and Standardbred industries. Enhancing fertility for the Thoroughbred and Standardbred industries. Responding to industry calls for improved methods of detecting and managing infertility in both stallions and mares, this project aims to provide a platform for competitive collaborations between universities, biotechnology companies and horse breeders: the end-users of technological developments in equine reproduction. The Thoroughbred and Standardbred bree ....Enhancing fertility for the Thoroughbred and Standardbred industries. Enhancing fertility for the Thoroughbred and Standardbred industries. Responding to industry calls for improved methods of detecting and managing infertility in both stallions and mares, this project aims to provide a platform for competitive collaborations between universities, biotechnology companies and horse breeders: the end-users of technological developments in equine reproduction. The Thoroughbred and Standardbred breeding industries contribute over $6.5 billion per annum to the Australian economy and employ thousands of staff across their value chains. However, these industries suffer from breeding program inefficiencies that amount to over $800 million in annual losses. Expected outcomes are novel reproductive technologies and specialised research capabilities that will make the Australian equine industry a global leader.Read moreRead less
Improving the efficiency of bovine oocyte maturation in vitro. For the dairy and beef industries, the hundreds of eggs (oocytes) in a high value cow's ovary that fail to produce a pregnancy are a wasted genetic resource. A key technology to unlocking this resource is in vitro maturation of oocytes, but the process is inefficient. One possible cause is that the current approaches to maturing oocytes in vitro do not adequately mimic the natural process in vivo. We will design new systems to matu ....Improving the efficiency of bovine oocyte maturation in vitro. For the dairy and beef industries, the hundreds of eggs (oocytes) in a high value cow's ovary that fail to produce a pregnancy are a wasted genetic resource. A key technology to unlocking this resource is in vitro maturation of oocytes, but the process is inefficient. One possible cause is that the current approaches to maturing oocytes in vitro do not adequately mimic the natural process in vivo. We will design new systems to mature cow oocytes in vitro by altering the chemical composition of maturation medium, thus improving the efficiency of laboratory embryo production and related technologies.Read moreRead less
Development of cloning technology for the Australian Pig Industry. Cloning has the potential to be the most efficient of the reproductive technologies developed for increasing genetic improvement in livestock. Currently up to 5% of cloned embryos develop to term in the pig. This is higher than that reported for cattle and sheep. Moreover the use of this technology in the pig does not appear not to result in the same sorts of problems and losses seen around the time of birth in these species ....Development of cloning technology for the Australian Pig Industry. Cloning has the potential to be the most efficient of the reproductive technologies developed for increasing genetic improvement in livestock. Currently up to 5% of cloned embryos develop to term in the pig. This is higher than that reported for cattle and sheep. Moreover the use of this technology in the pig does not appear not to result in the same sorts of problems and losses seen around the time of birth in these species i.e. the majority of cloned pigs appear normal and are healthy at birth. However before cloning can be used commercially, current efficiencies need to be increased approx two fold for this to be economically viable. The aim of the present study is to improve the efficiency of our current cloning protocol and develop associated technologies such as embryo freezing to facilitate commercialisation. This will ensure that the Australian Pig Industry remains competitive at a pivotal time in its development.Read moreRead less
Quantifying the effect of nutrient-gene interaction in utero in key tissues instrumental to productivity and sustainability of the beef industry. The cattle industry occupies 43 per cent of the land mass and provides 13 per cent of rural employment. Nutritional insult in utero affects postnatal reproductive and production traits in cattle and imposes epigenetic modifications. This project investigates the effect upon genes affecting appetite, ovarian development, adipogenesis, myogenesis and pos ....Quantifying the effect of nutrient-gene interaction in utero in key tissues instrumental to productivity and sustainability of the beef industry. The cattle industry occupies 43 per cent of the land mass and provides 13 per cent of rural employment. Nutritional insult in utero affects postnatal reproductive and production traits in cattle and imposes epigenetic modifications. This project investigates the effect upon genes affecting appetite, ovarian development, adipogenesis, myogenesis and post natal growth.Read moreRead less
An integrated genomics approach to improve our understanding of the biology of genital campylobacteriosis in beef cattle. Beef is Australia's most valuable agricultural export estimated at $9.6B annually and this industry accounts for one-third of full time employment in regional communities. Currently, unknown causes of reproductive losses in cattle in Northern Australian cost producers approximately $15M per annum. Bovine genital campylobacteriosis is one of the major risk factors associated w ....An integrated genomics approach to improve our understanding of the biology of genital campylobacteriosis in beef cattle. Beef is Australia's most valuable agricultural export estimated at $9.6B annually and this industry accounts for one-third of full time employment in regional communities. Currently, unknown causes of reproductive losses in cattle in Northern Australian cost producers approximately $15M per annum. Bovine genital campylobacteriosis is one of the major risk factors associated with this high cost due to the inability to accurately diagnose the disease. This research will integrate genomics and bioinformatics with infection and vaccination models to improve our understanding of the biology of the disease to develop appropriate control strategies securing Australia's beef industry.Read moreRead less
In vitro prediction tests of fertility of frozen semen of sheep and cattle. Variation in fertility after artificial insemination (AI) of frozen semen is high and leads to economic loss. Routine assessment of the quality of frozen semen relies on crude, simple tests of motility and morphology of sperm. More sophisticated sperm ultrastructural and function tests have recently been developed, but it is not known how these tests relate to fertility in the field. In this project we will test a ran ....In vitro prediction tests of fertility of frozen semen of sheep and cattle. Variation in fertility after artificial insemination (AI) of frozen semen is high and leads to economic loss. Routine assessment of the quality of frozen semen relies on crude, simple tests of motility and morphology of sperm. More sophisticated sperm ultrastructural and function tests have recently been developed, but it is not known how these tests relate to fertility in the field. In this project we will test a range of in vitro methods of assessment of motility and morphology of sperm and compare them with fertility of frozen semen samples after AI, leading to more objective methods of semen analysis.Read moreRead less
An investigation of the underlying mechanisms that control gender and fertility in the Moreton Bay Bug, Thenus orientalis. To date there is no available information on the underlying biochemical and genetic mechanisms that control gender and fertility in Thenus spp. Understanding how gender and fertility are controlled in this lobster species will allow development of technologies to exploit desired commercial traits such as reproductive sterility (for genetic protection). Such technologies wil ....An investigation of the underlying mechanisms that control gender and fertility in the Moreton Bay Bug, Thenus orientalis. To date there is no available information on the underlying biochemical and genetic mechanisms that control gender and fertility in Thenus spp. Understanding how gender and fertility are controlled in this lobster species will allow development of technologies to exploit desired commercial traits such as reproductive sterility (for genetic protection). Such technologies will facilitate rapid uptake of commercial culture of this high-value species in Australia and establishment of international markets. Read moreRead less