Chemical inhibition: a new approach to investigate the role of a key protease, CtHtrA, from Chlamydia trachomatis. Infertility in women frequently results from infection with Chlamydia trachomatis. This project will develop an inhibitor compound against a important protein from this bacteria. This will establish a new scientific approach to study Chlamydia trachomatis. This project will also contribute to the development of new treatments for infertility.
Stuctural analysis of RNA polymerase elongation complexes. RNA polymerase (RNAP) is an essential enzyme in all living cells. Its role is to convert the genetic information stored in genes into a message that can be converted into protein. Many additional factors are required to ensure that this enzyme functions correctly in the cell. The aim of this project is to obtain structural information on a bacterial RNAP complexed with an essential transcription factor called NusA. Using this information ....Stuctural analysis of RNA polymerase elongation complexes. RNA polymerase (RNAP) is an essential enzyme in all living cells. Its role is to convert the genetic information stored in genes into a message that can be converted into protein. Many additional factors are required to ensure that this enzyme functions correctly in the cell. The aim of this project is to obtain structural information on a bacterial RNAP complexed with an essential transcription factor called NusA. Using this information, plus data already obtained on the structure of this enzyme complexed with another essential factor called sigma, we will design small molecules to inhibit the interaction of these essential factors with polymerase. These molecules will serve as leads for the development of new antibiotics.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100127
Funder
Australian Research Council
Funding Amount
$355,000.00
Summary
Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein funct ....Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein function inside living cells. The new equipment is designed to provide a fast super-resolution imaging system to study the intracellular dynamics of proteins in vitro and a super-resolution microscope to visualise structures and assemblies inside microbes with a resolution of tens of nanometres, putting in vitro biochemistry into the context of a living cell. Read moreRead less
Determinants of substrate preferences and environmental applications of the copper membrane monooxygenases. The project aims to improve sustainability of environmental problems related to methane emissions, nitrogen cycling and pollution. We are developing tools targeting microbial genes correlated to all these issues. Data from these tests provides the information needed for monitoring environmental health and development of sustainable solutions.