Using defined biotic and abiotic stimuli to dissect patterns of gene expression and protein accumulation that specify root architecture. Root morphogenesis is fundamental to agriculture and valuable for investigating the informational networks of genes, proteins and metabolites that control root growth and plant development. Root systems vary widely both within and between species. Root morphology is directed by a basic genetic program that is influenced by environmental factors to provide an e ....Using defined biotic and abiotic stimuli to dissect patterns of gene expression and protein accumulation that specify root architecture. Root morphogenesis is fundamental to agriculture and valuable for investigating the informational networks of genes, proteins and metabolites that control root growth and plant development. Root systems vary widely both within and between species. Root morphology is directed by a basic genetic program that is influenced by environmental factors to provide an enormous "phenotypic plasticity". This project will use two model plant systems to investigate how different external signals are "translated" by the plant into different developmental regimes. This knowledge is crucial to understanding how the plasticity of root development is modulated in response to changing environmental factors.Read moreRead less
A multidisciplinary research program to assess limiting factors and predict impacts of climate change for endangered Australian orchids. Climate change poses a significant threat to biodiversity. Australian sexually deceptive orchids are dependent on obligate and specialised interactions with pollinators and fungi. Consequently, they may face a high risk of extinction if climate change uncouples these interactions. Thus orchids provide an important bio-indicator of change. The tools and expertis ....A multidisciplinary research program to assess limiting factors and predict impacts of climate change for endangered Australian orchids. Climate change poses a significant threat to biodiversity. Australian sexually deceptive orchids are dependent on obligate and specialised interactions with pollinators and fungi. Consequently, they may face a high risk of extinction if climate change uncouples these interactions. Thus orchids provide an important bio-indicator of change. The tools and expertise developed will contribute directly to the conservation of endangered Australian orchids, and will take into account the risks of climate change. The project will contribute to the priority research goals of sustainable use of Australia's biodiversity as well as responding to climate change and variability. The project will also provide high quality, cross-disciplinary training.Read moreRead less
The role of the Ttyh1 protein in cell activation. We have cloned TTYH1, a human homologue of the Drosophila melanogaster tweety gene. The mouse gene has also been identified. The predicted structure of the protein is a membrane protein with 5 transmembrane domains. We have also expressed a GFP-tagged fusion protein in mouse fibroblasts. Confocal microscopy indicates that this protein is likely to be a novel adhesion molecule, with a cellular distribution characteristic of molecules such as integ ....The role of the Ttyh1 protein in cell activation. We have cloned TTYH1, a human homologue of the Drosophila melanogaster tweety gene. The mouse gene has also been identified. The predicted structure of the protein is a membrane protein with 5 transmembrane domains. We have also expressed a GFP-tagged fusion protein in mouse fibroblasts. Confocal microscopy indicates that this protein is likely to be a novel adhesion molecule, with a cellular distribution characteristic of molecules such as integrins. We aim to determine the function of Ttyh1, its interacting intra- and extra-cellular proteins and to assess its candidature as a molecule of importance in cell migration and adhesion.Read moreRead less
Evolution of halophytes: a phyloinformatic approach to understanding and exploiting the traits underlying salt-tolerance in plants. Salinity is an increasing burden on the Australian economy & environment, with >2 million ha of salt-affected land, at an annual cost to agriculture over $187 million. One solution is to exploit naturally salt-tolerant plants to increase productive agricultural land and restore salt-affected environments. To do this, we must increase basic knowledge of the diversity ....Evolution of halophytes: a phyloinformatic approach to understanding and exploiting the traits underlying salt-tolerance in plants. Salinity is an increasing burden on the Australian economy & environment, with >2 million ha of salt-affected land, at an annual cost to agriculture over $187 million. One solution is to exploit naturally salt-tolerant plants to increase productive agricultural land and restore salt-affected environments. To do this, we must increase basic knowledge of the diversity & distribution of salt-tolerance. This project is the first to use DNA sequences from thousands of species to understand the evolution of salt-tolerance in order to provide the foundation for the development of new crop varieties, selection of species that can be developed for bioremediation, and identification of traits that will be profitable targets for breeding programs. Read moreRead less
The molecular basis for oocyst and cyst wall formation in apicomplexan parasites. Apicomplexan parasites such as Eimeria, Neospora, Toxoplasma and Plasmodium are single celled organisms - protozoa - that cause some of the most serious infectious diseases of livestock and humans ever known. Transmission of these parasites is dependent on their ability to encase themselves in protective structures known as oocyst or cyst walls. These walls are resistant to harsh environmental conditions, chemicals ....The molecular basis for oocyst and cyst wall formation in apicomplexan parasites. Apicomplexan parasites such as Eimeria, Neospora, Toxoplasma and Plasmodium are single celled organisms - protozoa - that cause some of the most serious infectious diseases of livestock and humans ever known. Transmission of these parasites is dependent on their ability to encase themselves in protective structures known as oocyst or cyst walls. These walls are resistant to harsh environmental conditions, chemicals and attack by the immune system. We will discover and characterise the molecular basis for cyst wall formation. This fundamental knowledge will be the building block for new, highly specific drugs and vaccines to control these extremely important pathogens.Read moreRead less
Control of meiosis and embryogenesis as a means to induce higher plants to reproduce asexually through seed. New plant meiosis-control genes will be isolated and characterised. These will be used, together with cell proliferation control genes characterised in the applicant's laboratory, to control gamete formation and embryo development in higher plants, and hence plant reproduction. This research will provide a platform for genetic fixation of hybrid vigour and repeat propagation of F1 hybrid ....Control of meiosis and embryogenesis as a means to induce higher plants to reproduce asexually through seed. New plant meiosis-control genes will be isolated and characterised. These will be used, together with cell proliferation control genes characterised in the applicant's laboratory, to control gamete formation and embryo development in higher plants, and hence plant reproduction. This research will provide a platform for genetic fixation of hybrid vigour and repeat propagation of F1 hybrid seed without attenuation of vigour. The technology will increase yield and profitability for Australian agriculture, and ensure access to technology that the industry partner will otherwise develop overseas. The technology benefits most major crops that are bred and grown to meet increasing demand for food and speciality products for pharmaceutical or industrial purposes.Read moreRead less
Why is the photosynthetic CO2-fixing enzyme, Rubisco, so inefficient? Dissection of the catalytic chemistry by computational simulation and experimental testing. Fixation of CO2 by the enzyme Rubisco during photosynthesis produces organic compounds which feed all life. Despite this critical role, Rubisco catalyses its reaction sluggishly and, worse, discriminates poorly between CO2 and O2, leading to useless products. Our combined expertise equips us to analyse Rubisco's mechanism using quantum- ....Why is the photosynthetic CO2-fixing enzyme, Rubisco, so inefficient? Dissection of the catalytic chemistry by computational simulation and experimental testing. Fixation of CO2 by the enzyme Rubisco during photosynthesis produces organic compounds which feed all life. Despite this critical role, Rubisco catalyses its reaction sluggishly and, worse, discriminates poorly between CO2 and O2, leading to useless products. Our combined expertise equips us to analyse Rubisco's mechanism using quantum-chemical methods and then test predictions experimentally. We will capitalise on our previous successful studies of Rubisco by addressing emergent issues which are the keys to understanding catalytic efficiency and CO2/O2 selectivity: the roles of a carbamylated lysine; the way CO2 addition is rendered irreversible; and the spin inversion inherent in O2 addition.Read moreRead less
Isolation and characterization of genes regulating female reproductive organ development in plants. Genes that regulate female reproductive organ development are of immense value for Australia as tools for seed improvement. Those from our preliminary screen have convinced our industry partners that they can be agents for engineering of apomixis or creation of fertile seed without fertilisation. This will allow the capture of hybrid vigour in wheat and rice, for which commercial hybrid seed prod ....Isolation and characterization of genes regulating female reproductive organ development in plants. Genes that regulate female reproductive organ development are of immense value for Australia as tools for seed improvement. Those from our preliminary screen have convinced our industry partners that they can be agents for engineering of apomixis or creation of fertile seed without fertilisation. This will allow the capture of hybrid vigour in wheat and rice, for which commercial hybrid seed production is not currently available. In wheat alone, apomixis presents for Australia an economic value of more than Aus$ ½ billion per annum. Furthermore, controlled apomixis will accelerate breeding programs that will bring drought resistance and minimal fertiliser requiring varieties to the farmer.Read moreRead less