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High temperature limits of leaf function. In arid and semi-arid central Australia, Acacia spp. dominate the over-storey, but this shifts to Eucalyptus and Corymbia spp. in more mesic coastal regions. Areas of central Australia are extremely hot, dry and sunny, and it is this combination of stresses that likely excludes Eucalyptus spp. from many landforms. There has been little research on high temperature tolerance of Acacia and Eucalyptus, despite the putative importance of this stress, in co ....High temperature limits of leaf function. In arid and semi-arid central Australia, Acacia spp. dominate the over-storey, but this shifts to Eucalyptus and Corymbia spp. in more mesic coastal regions. Areas of central Australia are extremely hot, dry and sunny, and it is this combination of stresses that likely excludes Eucalyptus spp. from many landforms. There has been little research on high temperature tolerance of Acacia and Eucalyptus, despite the putative importance of this stress, in combination with other stresses, in limiting species? distributions. Our program of collaborative research will examine the tolerance of Acacia and Eucalyptus to a combination of high temperatures, drought and high light.Read moreRead less
A microscopical examination of curdlan production by an Agrobacterium sp. We will investigate the secretion of the insoluble polysaccharide curdlan, a (1,3)-beta-glucan, from the surfaces of Agrobacterium cells and the assembly of the individual polysaccharide chains into microfibrils. Using state-of-the-art techniques in time lapse and electron microscopy we will compare the images of wild type curdlan-producing cells with those of mutants impaired in the production of curdlan. The outputs will ....A microscopical examination of curdlan production by an Agrobacterium sp. We will investigate the secretion of the insoluble polysaccharide curdlan, a (1,3)-beta-glucan, from the surfaces of Agrobacterium cells and the assembly of the individual polysaccharide chains into microfibrils. Using state-of-the-art techniques in time lapse and electron microscopy we will compare the images of wild type curdlan-producing cells with those of mutants impaired in the production of curdlan. The outputs will be information on the mechanics of curdlan production that will complement that emerging from our molecular biological and biochemical studies. These will have implications for understanding bacterial polysaccharide production in general and may have a commercial outcome in enhanced curdlan production.Read moreRead less
A high-through-put method for unlocking the mitochondrial genomes of significant pathogens. The national/community benefits of this research are: (1) to develop a long-term, high quality scientific and technological program contributing to national objectives, including the maintenance of a strong capability in basic research, the development of new scientific concepts and the enhancement of international collaborative links; (2) to strengthen the links between basic and applied research; (3) to ....A high-through-put method for unlocking the mitochondrial genomes of significant pathogens. The national/community benefits of this research are: (1) to develop a long-term, high quality scientific and technological program contributing to national objectives, including the maintenance of a strong capability in basic research, the development of new scientific concepts and the enhancement of international collaborative links; (2) to strengthen the links between basic and applied research; (3) to develop excellence in research by promoting collaborative research, resulting in a more efficient use of resources in a national and international context; (4) to enhance the skills-base in biology and biotechnology; and (5) to substantially increase global visibility through quality research, leading to an increased investment in Australian science.Read moreRead less
Regulation of proteolysis by specialised adaptor proteins. Training research scientists of the future forms an integral part of this research program and this collaboration will provide an excellent opportunity for young Australian scientists to be exposed to the very professional and competitive environment of basic research, as it exists in Germany. It will expose early career researchers to new ideas and emerging methodologies arming them with valuable skills, which they will transfer to Aust ....Regulation of proteolysis by specialised adaptor proteins. Training research scientists of the future forms an integral part of this research program and this collaboration will provide an excellent opportunity for young Australian scientists to be exposed to the very professional and competitive environment of basic research, as it exists in Germany. It will expose early career researchers to new ideas and emerging methodologies arming them with valuable skills, which they will transfer to Australia. The involvement of Prof. Turgay in the Deutsche Forschungsgemeinschaft (DFG) Priority Programme: Proteolysis in Prokaryotes also provides a unique opportunity for these young researchers to interact with several of the worlds leading scientists in the area of proteolysis, enhancing Australia's reputation at the forefront of science.Read moreRead less
Structure and function of novel transporters in alphaproteobacteria. First, detailed knowledge of a set of membrane transporters and the way their activity might be inhibited, will have implications for the treatment of human disease. Second, excellent outcomes are provided for the training of postgraduate students and research staff. This project entails cutting edge technology, and the transfer of technical capabilities not currently available in Australia. Third, our studies on non-pathogenic ....Structure and function of novel transporters in alphaproteobacteria. First, detailed knowledge of a set of membrane transporters and the way their activity might be inhibited, will have implications for the treatment of human disease. Second, excellent outcomes are provided for the training of postgraduate students and research staff. This project entails cutting edge technology, and the transfer of technical capabilities not currently available in Australia. Third, our studies on non-pathogenic species of alpha-proteobacteria provides for a timely advance in our knowledge of their biology: other species of alpha-proteobacteria were amongst the first organisms trialled for biological weapons by the USA and the former Soviet Union, and those pathogenic species are rated as Class 3 organisms.Read moreRead less
Functional pathways in primate retina. When light enters the eye, specialised nerve cells called photoreceptors transform the light signal into nerve signals. These signals are processed in the retina by two groups of neurones, bipolar and ganglion cells before being sent to higher brain centres. Bipolar and ganglion cells can be subdivided into several types. We will measure which types communicate with each other and which proteins (neurotransmitter receptors) are involved at the site of commu ....Functional pathways in primate retina. When light enters the eye, specialised nerve cells called photoreceptors transform the light signal into nerve signals. These signals are processed in the retina by two groups of neurones, bipolar and ganglion cells before being sent to higher brain centres. Bipolar and ganglion cells can be subdivided into several types. We will measure which types communicate with each other and which proteins (neurotransmitter receptors) are involved at the site of communication between these nerve cells. This study will improve our knowledge of the basis of functional subspecialisation at the earliest stages of the visual process.Read moreRead less
DNA Dynamics is Shear and Extensional Flows: Simulation and Single Molecule Experiments. The proposal seeks to establish a collaboration between Monash University and Stanford University in order to combine several recent experimental and theoretical advances that have been made by the individual groups in single molecule experimental techniques, extensional rheometry, and molecular rheology, to obtain new insights into the structure and dynamics of biopolymers. The central aim is to make a sign ....DNA Dynamics is Shear and Extensional Flows: Simulation and Single Molecule Experiments. The proposal seeks to establish a collaboration between Monash University and Stanford University in order to combine several recent experimental and theoretical advances that have been made by the individual groups in single molecule experimental techniques, extensional rheometry, and molecular rheology, to obtain new insights into the structure and dynamics of biopolymers. The central aim is to make a significant contribution towards bringing state-of-the-art techniques used for the characterization of polymeric systems to bear on the nature and origin of the elastic properties of biopolymers.Read moreRead less
A unified approach to studies on mitochondrial biogenesis. Mitochondria supply the cell with the majority of its energy as well as performing other key functions. How mitochondrial processes are regulated within the cell is an important area of biology. The broad aims of this collaboration are to analyse processes involved in mitochondrial biogenesis using yeast and mammalian cells as model systems. In particular the protein import processes and mitochondrial stress responses will be studied. Th ....A unified approach to studies on mitochondrial biogenesis. Mitochondria supply the cell with the majority of its energy as well as performing other key functions. How mitochondrial processes are regulated within the cell is an important area of biology. The broad aims of this collaboration are to analyse processes involved in mitochondrial biogenesis using yeast and mammalian cells as model systems. In particular the protein import processes and mitochondrial stress responses will be studied. The outcomes of this work will enable us to gain an appreciation of the similarities and differences in processes that exist between organisms so that general, unifying models can be made.Read moreRead less
Joint Theoretical and Experimental Electron Momentum Spectroscopic Studies for DNA Bases. The study of DNA structure is an area of intense research activity and continues to reveal new levels of complexity and diversity. Recent experiments (Science, 2002) provided direct evidences of the adenine non-planarity, indicating non-rigidity of DNA bases. Electron momentum spectroscopy (EMS) has been identified to be an appropriate technique in the study of chemical binding mechanism and orbitals at mol ....Joint Theoretical and Experimental Electron Momentum Spectroscopic Studies for DNA Bases. The study of DNA structure is an area of intense research activity and continues to reveal new levels of complexity and diversity. Recent experiments (Science, 2002) provided direct evidences of the adenine non-planarity, indicating non-rigidity of DNA bases. Electron momentum spectroscopy (EMS) has been identified to be an appropriate technique in the study of chemical binding mechanism and orbitals at molecular level. The aims of the project is to study orbitals and interactions of DNA and RNA bases such as adenine, thymine (uracil), guanine and cytosine using momentum space quantum mechanics and EMS experimental techniques. The outcome of the project will improve our understanding of the DNA double helical strand structure.Read moreRead less
Relationship of the functional architecture of the mammalian brain to its microcircuitry. The project seeks to understand a very fundamental issue in neuroscience: how the connectivity and architecture of the cortex are related to the functions of neurones in that area? This will be investigated by imaging the surface of the visual cortex of anaesthetised cats and monkeys as special visual patterns are shown to the eye/s. The overall picture gained of the active and inactive cortical areas will ....Relationship of the functional architecture of the mammalian brain to its microcircuitry. The project seeks to understand a very fundamental issue in neuroscience: how the connectivity and architecture of the cortex are related to the functions of neurones in that area? This will be investigated by imaging the surface of the visual cortex of anaesthetised cats and monkeys as special visual patterns are shown to the eye/s. The overall picture gained of the active and inactive cortical areas will be related to the properties of neurones in those areas and to those of individual input and output fibres. An optical imaging equipment will be acquired in 2004 using a recently awarded LIEF grant to the CI.Read moreRead less