ORCID Profile
0000-0002-1686-2989
Current Organisation
Forensic Science South Australia
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Publisher: American Vacuum Society
Date: 11-2020
DOI: 10.1116/6.0000586
Abstract: The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable the rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed mechanistic knowledge of specific material-virion interactions before they can be employed, for ex le, to aid in the purification of vaccine components or in the design of a more effective PPE. Here, we show that an adaption of a polymer microarray method for screening bacterial-surface interactions allows for the screening of polymers for desirable material-virion interactions. Nonpathogenic virus-like particles including fluorophores are exposed to the arrays in an aqueous buffer as a simple model of virions carried to the surface in saliva/sputum. Competitive binding of Lassa and Rubella virus-like particles is measured to probe the relative binding properties of a selection of copolymers. This provides the first step in the development of a method for the discovery of novel materials with promise for viral binding, with the next being development of this method to assess absolute viral adsorption and assessment of the attenuation of the activity of live virus, which we propose would be part of a material scale up step carried out in high containment facilities, alongside the use of more complex media to represent biological fluids.
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D3DT01378B
Abstract: Cubic coordination cages encapsulate P–F containing guests and, when complexed, protect the P–F bond from aqueous hydrolysis.
Publisher: SAGE Publications
Date: 09-07-2014
Publisher: Springer Science and Business Media LLC
Date: 31-05-2017
DOI: 10.1038/S41598-017-02470-0
Abstract: A new urea functionalised 4-amino-1,8-naphthalimide based fluorescent anion sensor was synthesised in 64% yield over three steps. Fluorescence and 1 H NMR titrations showed that the sensor complexes strongly with acetate and dihydrogen phosphate and to a lesser extent bromide. The corresponding binding stoichiometries were examined using 1 H NMR titrations. Results show that the sensor molecule initially forms 1:1 complexes through hydrogen bonding to the urea moiety, followed by secondary complexation to form higher order host:guest stoichiometries. Specifically, oxyanions complex to the sensor via hydrogen bonding through synergistic aryl C-H and N-H anion interactions in a 1:2 sensor:oxyanion arrangement. Furthermore, 2:1 sensor:oxyanion complexes are formed through an oxyanion linkage between two urea functionalities on different host molecules. This contrasts the majority of previous reports for similar hosts, which indicate 1:1 binding stoichiometry.
Publisher: Elsevier BV
Date: 10-2014
Publisher: Springer Science and Business Media LLC
Date: 23-10-2020
DOI: 10.1038/S41598-020-75226-Y
Abstract: Protein microarrays have been successfully used for detection of allergen-specific IgE in patient sera. Here, we demonstrate proof-of-concept of a solid-phase technique coupling the high-throughput potential of protein microarrays with the biologically relevant readout provided by IgE reporter cells, creating a novel allergic sensitization detection system. Three proteins (κ-casein, timothy grass pollen extract, polyclonal anti-human IgE) were printed onto three different polymer-coated surfaces (aldehyde-, epoxy- and NHS ester-coated). ToF–SIMs analysis was performed to assess printed protein stability and retention during washing steps. NFAT-DsRed rat basophil leukemia cell attachment and retention during washing steps was assessed after treatment with various extracellular matrix proteins. NFAT-DsRed IgE reporter cells were sensitized with serum of an allergic donor, incubated on the printed slides, and cell activation determined using a microarray laser scanner. NFAT DsRed IgE reporter cell binding was significantly increased on all polymer surfaces after incubation with fibronectin and vitronectin, but not collagen or laminin. All surfaces supported printed protein stability during washing procedure, with epoxy- and NHS ester-coated surfaces showing best protein retention. Cell activation was significantly higher in NHS ester-coated slides after timothy grass pollen extract stimulation appearing a suitable substrate for further development of an automated allergy diagnosis system.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6RA07678E
Abstract: Using a simple dip-coating mechanism, urinary catheters have been coated with poly(2-methacryloyloxyethyl)trimethylammonium chloride (pMTAC) using activator regenerated by electron transfer (ARGET)–atom transfer radical polymerization (ATRP).
Publisher: IOP Publishing
Date: 25-02-2019
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5RA09370H
Abstract: The use of a polydopamine-based macroinitiator provides a flexible attachment method that is virtually independent of membrane substrate. The subsequent ARGET-ATRP controllably grafts the stable biofouling resistant polyzwitterion coating.
Publisher: American Chemical Society (ACS)
Date: 06-11-2020
Publisher: American Chemical Society (ACS)
Date: 09-07-2020
Publisher: Springer US
Date: 2020
Publisher: Elsevier BV
Date: 10-2015
Location: Malaysia
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Andrew Blok.