ORCID Profile
0000-0002-4576-2944
Current Organisation
University of Queensland Diamantina Institute for Cancer Immunology and Metabolic Medicine
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Publications
CLEC4C gene expression can be used to quantify circulating plasmacytoid dendritic cells
Publisher: Elsevier BV
Date: 2018
DOI: 10.1016/J.JIM.2018.11.001
Abstract: Plasmacytoid dendritic cells (pDC) are an important type I interferon producer that play an important role in the first line of host defence during viral infection. Abnormalities in pDC numbers and function have been associated with several health conditions. Quantifying pDC is important for understanding pDC related immune responses in viral infections and other diseases, however the current methods for quantifying pDC using flow cytometry have limited utility in large cohort studies involving multiple centres with limited access to flow cytometry. We reasoned that examining gene expression of the pDC marker C-type lectin domain family 4 member C (CLEC4C, also known as CD303 and BDCA2) in combination with pDC exclusive leukocyte immunoglobulin like receptor A4 (LILRA4, also known as CD85g and ILT7) might provide a more practical method that could be applied to multi-centre studies. Our results show a moderate correlation between pDC numbers measured by surface staining and CLEC4C gene expression in whole blood (rho = 0.39, P = .037, as well as a high correlation between CLEC4C gene expression in whole blood and peripheral blood mononuclear cells (rho = 0.79, P < .001). LILRA4 gene expression did not provide additional useful information. Our results indicate that measuring CLEC4C gene expression can provide an alternative method for quantifying pDC numbers in human s les.
Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
Publisher: Wiley
Date: 2021
DOI: 10.1002/CTI2.1356
Abstract: Viral respiratory infections cause considerable morbidity and economic loss. While rhinoviruses (RV) typically cause little more than the common cold, they can produce severe infections and disease exacerbations in susceptible in iduals, such as those with asthma. Variations in the regulation of key antiviral cytokines, particularly type I interferon (IFN‐α and IFN‐β), may contribute to RV susceptibility. To understand this variability, we compared the transcriptomes of high and low type I IFN producers. Blood mononuclear cells from 238 in iduals with or without asthma were cultured in the presence or absence of RV. Those s les demonstrating high or low RV‐stimulated IFN‐α production (N = 75) underwent RNA‐sequencing. Gene expression patterns were similar in s les from healthy participants and those with asthma. At baseline, the high IFN‐α producer group showed higher expression of genes associated with plasmacytoid dendritic cells, the innate immune response and vitamin D activation, but lower expression of oxidative stress pathways than the low IFN‐α producer group. After RV stimulation, the high IFN‐α producer group showed higher expression of genes found in immune response biological pathways and lower expression of genes linked to developmental and catabolic processes when compared to the low IFN‐α producer group. These differences suggest that the high IFN‐α group has a higher level of immune system readiness, resulting in a more intense and perhaps more focussed pathogen‐specific immune response. These results contribute to a better understanding of the variability in type I IFN production between in iduals.
Gremlin-1 associates with fibrillin microfibrils in vivo and regulates mesothelioma cell survival through transcription factor slug
Publisher: Springer Science and Business Media LLC
Date: 26-08-2013
An examination of the medicinal potential of Scaevola spinescens: Toxicity, antibacterial, and antiviral activities
Publisher: EManuscript Technologies
Date: 2011
Risks for cold frequency vary by sex: role of asthma, age, TLR7 and leukocyte subsets
Publisher: European Respiratory Society (ERS)
Date: 08-06-2020
DOI: 10.1183/13993003.02453-2019
Abstract: Viral respiratory infections are usually benign but can trigger asthma exacerbations. The factors associated with upper respiratory tract infection (cold) frequency are not fully understood, nor is it clear whether such factors differ between women and men. To determine which immunological and clinical variables associate with the frequency of self-reported respiratory infections (colds), 150 asthma cases and 151 controls were recruited. Associations between antiviral immune response variables: toll-like receptor (TLR)7/8 gene expression, plasmacytoid dendritic cell (pDC) numbers and interferon-α, tumour necrosis factor and interleukin-12 production, and asthma were then examined that might explain cold frequency. People with asthma cases reported more colds per year (median 3 versus 2 p .001) and had lower baseline TLR7 gene expression (odds ratio 0.12 p=0.02) than controls. Associations between many variables and cold frequency differed between women and men. In women, high blood neutrophil counts (β=0.096, p=0.002), and younger age (β=−0.017, p .001), but not exposure to children, were independently associated with more frequent colds. In men, low TLR7 expression (β=−0.96, p=0.041) and high CLEC4C gene expression (a marker of pDC β=0.88, p=0.008) were independently associated with more frequent colds. Poor asthma symptom control was independently associated with reduced TLR8 gene expression (β=−1.4, p=0.036) and high body mass index (β=0.041, p=0.004). Asthma, age and markers of inflammation and antiviral immunity in peripheral blood are associated with frequent colds. Interestingly, the variables associated with cold frequency differed between women and men.
Interleukin 33 Selectively Augments Rhinovirus-Induced Type 2 Immune Responses in Asthmatic but not Healthy People
Publisher: Frontiers Media SA
Date: 16-08-2018
Related Organisations
University Of Queensland Diamantina Institute For Cancer Immunology And Metabolic Medicine
Location: Australia
Related Funding Activities
No related grants have been discovered for Liisa Maarit Murray.