ORCID Profile
0000-0003-0581-1817
Current Organisations
University of Adelaide
,
University of South Australia
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Publisher: CSIRO Publishing
Date: 1976
DOI: 10.1071/AR9760845
Abstract: An experiment IS dcsci bed which lasted for SIX seasons and was designed to investigate the response of the cultivar Qouchen (syn Clare Riesling) to various trellising and pruning treatments This cultivar, which achieves high levels of bud fruitfulness when grown In the Murray Valley, responded to widening the trellis from 0.3 m to 1.4 m with an increase In yield of 25–30%. This yield Increase was largely due to a better bud burst for vines on the wider trellis. Increasing the number of nodes per vine from 32 to 48 caused a yield Increase of about 15 %, but a further Increase to 64 nodes d i d not result in any additional yield response In the initial year of the trial, spur- and cane-pruned vines (with node numbers in the ratio of 4 to 5) yielded equally well. But In later years spur-pruned vines gave increasingly better yields, again resulting from differences In bud burst. To investigate the relationship between wine quality and yield, the fruit of four combinations of pruning and trellising treatments, giving a range of yields, was made into wine. Small-scale wine-making techniques were used No significant differences In wine quality attributable to the yield differences were found.
Publisher: CSIRO Publishing
Date: 1978
DOI: 10.1071/EA9780847
Abstract: Trellis drying of Sultana grapes necessitates the severing of the fruit bearing canes from the vine at harvest time (Harvest pruning, HP). Two experiments have shown that HP of young vines, from the beginning of cropping onwards, caused no greater yield losses than those reported for mature vines, viz 10 per cent. In one experiment, HP gave significant yield reductions over six seasons, amounting to about 7 per cent, apparently due to fewer berries per bunch. Vines harvest pruned only every second year suffered losses mainly in the season following HP. In the other experiment, HP did not cause any loss of yield over four seasons. With or without HP, vines on a 0.9 m and 1.2 m wide T-trellis yielded about 20 per cent more than vines on a 0.3 m trellis, apparently because of better bud burst. Providing vines with two trunks, each producing the crop or replacement shoots only in alternate seasons, did not prevent HP losses. Such vines yielded similarly to one-trunk vines while vines with one trunk and planted in pairs yielded less. A 'split system' of training was used where each vine had all fruiting canes placed on one trellis wire whilst spurs producing all replacement shoots were trained on the other wire, the arrangement alternating down the row from vine to vine. HP of such vines was more than twice as fast as that of conventionally trained vines other management advantages are discussed.
Publisher: Elsevier BV
Date: 07-2019
Publisher: American Chemical Society (ACS)
Date: 24-02-2010
DOI: 10.1021/LA904443S
Abstract: Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been used as a technique to correlate the surface chemistry of chalcopyrite particles with their contact angle. Three particle sizes (20-38, 75-105, and 150-210 microm) were used, covering a range of contact angles between 20 and 90 degrees. Multivariate statistical techniques were applied to the ToF-SIMS data in order to identify structure in the data and the surface species contributing the most to surface chemistry and hence the hydrophobicity variation. A method to calculate the contact angle of chalcopyrite by ToF-SIMS surface analysis has been developed using only information from three secondary ions: oxygen, sulfur, and a thiol collector fragment. This approach is capable of determining the surface chemistry contribution to the contact angle of in idual mineral particles and the distribution of contact angles within a large ensemble of particles. Further measurements verified that the methodology can also be applied to flat surfaces, enabling rapid surface chemistry-hydrophobicity correlations to be made on a wide range of mineral and material systems.
Publisher: Springer Science and Business Media LLC
Date: 19-05-2023
Publisher: Wiley
Date: 22-07-2020
DOI: 10.1111/PCE.13841
Publisher: Institute of Mathematical Statistics
Date: 2012
DOI: 10.1214/12-EJS732
Publisher: Wiley
Date: 12-05-2023
Abstract: Comparative experiments involve the allocation of treatments to units, ideally by randomization. This necessarily confounds treatment information with unit information, which we distinguish from the other forms of information blending, in particular aliasing and marginality. We outline a factor‐allocation paradigm for describing experimental designs with the aim of (i) exhibiting the confounding in a design, using analysis‐of‐variance‐like tables, so as to understand and evaluate the design and (ii) formulating a linear mixed model based on the factor allocation that the design involves. The approach exhibits the dispersal of treatments information between units sources, allows designers a choice in the strategy that they adopt for including block‐treatment interactions, clarifies differences between experiments, accommodates systematic allocation of factors, and provides a consolidated analysis of nonorthogonal designs. It provides insights into the process of designing experiments and issues that commonly arise with designs. The paradigm has pedagogical advantages and is implemented using the R package dae .
Publisher: MDPI AG
Date: 11-2019
Abstract: Grain protein content (GPC) is a key quality attribute and an important marketing trait in wheat. In the current cropping systems worldwide, GPC is mostly determined by nitrogen (N) fertilizer application. The objectives of this study were to understand the differences in N response between high and low GPC wheat genotypes, and to assess the value of biomass growth analysis to assess the differences in N response. Six wheat genotypes from a range of high to low GPC were grown in low, medium and high N, under glasshouse conditions. This experiment was designed around non-destructive estimation of biomass using a high throughput image-based phenotyping system. Results showed that Spitfire and Mace had higher grain N% than Gazelle and QAL2000, and appeared to demand more N to grow their biomass. Moreover, at low N, Spitfire grew faster and achieved the maximum absolute growth rate earlier than high N-treated plants. High grain N% genotypes seem able to manage grain N reserves by compromising biomass production at low N. This study also indicated the importance of biomass growth analysis to show the differences in the N responsiveness of high and low GPC wheat.
Publisher: Elsevier BV
Date: 2020
DOI: 10.1016/J.PLANTSCI.2019.05.009
Abstract: Current climate change models project that water availability will become more erratic in the future. With soil nitrogen (N) supply coupled to water availability, it is important to understand the combined effects of variable water and N supply on food crop plants (above- and below-ground). Here we present a study that precisely controls soil moisture and compares stable soil moisture contents with a controlled wetting-drying cycle. Our aim was to identify how changes in soil moisture and N concentration affect shoot-root biomass, N acquisition in wheat, and soil N cycling. Using a novel gravimetric platform allowing fine-scale control of soil moisture dynamics, a 3 × 3 factorial experiment was conducted on wheat plants subjected to three rates of N application (0, 25 and 75 mg N/kg soil) and three soil moisture regimes (two uniform treatments: 23.5 and 13% gravimetric moisture content (herein referred to as Well-watered and Reduced water, respectively), and a Variable treatment which cycled between the two). Plant biomass, soil N and microbial biomass carbon were measured at three developmental stages: tillering (Harvest 1), flowering (Harvest 2), and early grain milk development (Harvest 3). Reduced water supply encouraged root growth when combined with medium and high N. Plant growth was more responsive to N than the water treatments imposed, with a 15-fold increase in biomass between the high and no added N treatment plants. Both uniform soil water treatments resulted in similar plant biomass, while the Variable water treatment resulted in less biomass overall, suggesting wheat prefers consistency whether at a Well-watered or Reduced water level. Plants did not respond well to variable soil moisture, highlighting the need to understand plant adaptation and biomass allocation with resource limitation. This is particularly relevant to developing irrigation practices, but also in the design of water availability experiments.
Publisher: Institute of Mathematical Statistics
Date: 06-2016
DOI: 10.1214/15-AOS1400
Publisher: Emerald
Date: 28-03-2008
Publisher: Oxford University Press (OUP)
Date: 30-05-2017
DOI: 10.1111/RSSA.12284
Publisher: Public Library of Science (PLoS)
Date: 23-07-2020
Publisher: MDPI AG
Date: 27-09-2022
Abstract: Salinity tolerance-associated phenotypes of 35 EMS mutagenized wheat lines originating from BARI Gom-25 were compared. Vegetative growth was measured using non-destructive image-based phenotyping. Five different NaCl concentrations (0 to 160 mM) were applied to plants 19 days after planting (DAP 19), and plants were imaged daily until DAP 38. Plant growth, water use, leaf Na+, K+ and Cl− content, and thousand kernel weight (TKW) were measured, and six lines were selected for further analysis. In saline conditions, leaf Na+, K+, and Cl− content variation on a dry weight basis within these six lines were ~9.3, 1.4, and 2.4-fold, respectively. Relative to BARI Gom-25, two (OA6, OA62) lines had greater K+ accumulation, three (OA6, OA10, OA62) had 50–75% lower Na+:K+ ratios, and OA62 had ~30% greater water-use index (WUI). OA23 had ~2.2-fold greater leaf Na+ and maintained TKW relative to BARI Gom-25. Two lines (OA25, OA52) had greater TKW than BARI Gom-25 when grown in 120 mM NaCl but similar Na+:K+, WUI, and biomass accumulation. OA6 had relatively high TKW, high leaf K+, and WUI, and low leaf Na+ and Cl−. Phenotypic variation revealed differing associations between the parameters measured in the lines. Future identification of the genetic basis of these differences, and crossing of lines with phenotypes of interest, is expected to enable the assessment of which combinations of parameters deliver the greatest improvement in salinity tolerance.
Publisher: Oxford University Press (OUP)
Date: 1988
Publisher: CSIRO Publishing
Date: 2017
DOI: 10.1071/FP17049
Abstract: Soil salinity can severely reduce crop growth and yield. Many studies have investigated salinity tolerance mechanisms in cereals using phenotypes that are relatively easy to measure. The majority of these studies measured the accumulation of shoot Na+ and the effect this has on plant growth. However, plant growth is reduced immediately after exposure to NaCl before Na+ accumulates to toxic concentrations in the shoot. In this study, nondestructive and destructive measurements are used to evaluate the responses of 24 predominately Australian barley (Hordeum vulgare L.) lines at 0, 150 and 250 mM NaCl. Considerable variation for shoot tolerance mechanisms not related to ion toxicity (shoot ion-independent tolerance) was found, with some lines being able to maintain substantial growth rates under salt stress, whereas others stopped growing. Hordeum vulgare spp. spontaneum accessions and barley landraces predominantly had the best shoot ion independent tolerance, although two commercial cultivars, Fathom and Skiff, also had high tolerance. The tolerance of cv. Fathom may be caused by a recent introgression from H. vulgare L. spp. spontaneum. This study shows that the most salt-tolerant barley lines are those that contain both shoot ion-independent tolerance and the ability to exclude Na+ from the shoot (and thus maintain high K+ : Na+ ratios).
Publisher: Springer Science and Business Media LLC
Date: 05-03-2016
Publisher: CSIRO Publishing
Date: 1998
DOI: 10.1071/EA97046
Abstract: Summary. A method for deriving the analysis of variance for an experiment is presented and applied to grazing trials. A special feature of grazing trials, specifically utilised by our method, is that they involve at least 2 randomisations: treatments are randomised to field units (for ex le paddocks or plots), and field units are randomised to animals. Randomisation results in the confounding (‘mixing up’) of terms and our method includes separate terms in the analysis of variance table for confounded terms so that all sources of variability in the experiment have terms for them included in the table and the confounding between the sources of variability in the experiment is explicitly displayed in the table. This information is used in determining the valid error terms and we will present ex les that show how to ascertain these for effects of interest and hence which effects can be tested. In this it fulfils the same role as the contentious process of identifying the experimental unit. It will be demonstrated that the inclusion of separate terms for confounded terms results in improper replication in grazing trials being automatically signalled, and makes its ramifications clear.
Publisher: Frontiers Media SA
Date: 30-10-2019
Publisher: MDPI AG
Date: 15-07-2019
Abstract: Living walls (LW) have been widely proposed as a form of green infrastructure to improve aesthetics, energy consumption, and microclimate in urban environments by adding densely-planted vegetation to the outside walls of buildings. Scientific studies using multiple treatments in a single LW face challenges due to the close physical proximity of different treatments, particularly the potential for plants above to influence those below. A study on a west-facing LW was undertaken to investigate 36 unique treatments in Adelaide, South Australia, for nine months. The LW comprised combinations of six native plant species, three soil substrates and two irrigation volumes. The LW consisted of 144 modular trays mounted on a wall in a 12 × 12 grid with four replicates of each treatment. The location of each treatment was designed to account for a cascading carry-over effect that may be present when one plant is placed above another. Carry-over effect of the model designed showed mixed results among the plant groups identified. It was also found that long-form plants can significantly shade smaller plants below them. Experimental research into the performance of plants in mixed species LW should consider the carry-over effect to account for this.
Publisher: Cold Spring Harbor Laboratory
Date: 17-08-2023
DOI: 10.1101/2023.08.15.553433
Abstract: Soil salinity is a major environmental stressor affecting agricultural productivity worldwide. Understanding plant responses to salt stress is crucial for developing resilient crop varieties. Wild relatives of cultivated crops, such as wild tomato, Solanum pimpinellifolium , can serve as a useful resource to further expand the resilience potential of the cultivated germplasm, S. lycopersicum . In this study, we employed high-throughput phenotyping in the greenhouse and field conditions to explore salt stress responses of a S. pimpinellifolium ersity panel. Our study revealed extensive phenotypic variations in response to salt stress, with traits such as transpiration rate, shoot mass, and ion accumulation showing significant correlations with plant performance. We found that while transpiration was a key determinant of plant performance in the greenhouse, shoot mass strongly correlated with yield under field conditions. Conversely, ion accumulation was the least influential factor under greenhouse conditions. Through a Genome Wide Association Study, we identified candidate genes not previously associated with salt stress, highlighting the power of high-throughput phenotyping in uncovering novel aspects of plant stress responses. This study contributes to our understanding of salt stress tolerance in S. pimpinellifolium and lays the groundwork for further investigations into the genetic basis of these traits, ultimately informing breeding efforts for salinity tolerance in tomato and other crops.
Publisher: Elsevier BV
Date: 09-2013
Abstract: A common practice in poultry science is to compare new treatments with a control or between treatments tested in planned experiments. The overall F-test from an ANOVA of the data allows the researcher to reject or not reject the null hypothesis. However, the correct conclusion from such analysis depends on sufficient replicates being included in the experiment. On the other hand, restrictions are imposed to reduce the number of birds used in experiments for welfare reasons and to save scarce resources. We review the basic concepts needed to determine the number of replicates before conducting an experiment. We use these concepts to assess the results of several real experiments and to show what might be done in future experiments. We describe how to do the computations in R software.
Publisher: Springer Science and Business Media LLC
Date: 10-03-2020
DOI: 10.1186/S13007-020-00577-6
Abstract: Non-destructive high-throughput plant phenotyping is becoming increasingly used and various methods for growth analysis have been proposed. Traditional longitudinal or repeated measures analyses that model growth using statistical models are common. However, often the variation in the data is inappropriately modelled, in part because the required models are complicated and difficult to fit. We provide a novel, computationally efficient technique that is based on smoothing and extraction of traits (SET), which we compare with the alternative traditional longitudinal analysis methods. The SET-based and longitudinal analyses were applied to a tomato experiment to investigate the effects on plant growth of zinc (Zn) addition and growing plants in soil inoculated with arbuscular mycorrhizal fungi (AMF). Conclusions from the SET-based and longitudinal analyses are similar, although the former analysis results in more significant differences. They showed that added Zn had little effect on plants grown in inoculated soils, but that growth depended on the amount of added Zn for plants grown in uninoculated soils. The longitudinal analysis of the unsmoothed data fitted a mixed model that involved both fixed and random regression modelling with splines, as well as allowing for unequal variances and autocorrelation between time points. A SET-based analysis can be used in any situation in which a traditional longitudinal analysis might be applied, especially when there are many observed time points. Two reasons for deploying the SET-based method are (i) biologically relevant growth parameters are required that parsimoniously describe growth, usually focussing on a small number of intervals, and/or (ii) a computationally efficient method is required for which a valid analysis is easier to achieve, while still capturing the essential features of the exhibited growth dynamics. Also discussed are the statistical models that need to be considered for traditional longitudinal analyses and it is demonstrated that the oft-omitted unequal variances and autocorrelation may be required for a valid longitudinal analysis. With respect to the separate issue of the subjective choice of mathematical growth functions or splines to characterize growth, it is recommended that, for both SET-based and longitudinal analyses, an evidence-based procedure is adopted.
Publisher: Wiley
Date: 03-1999
Publisher: Springer Science and Business Media LLC
Date: 07-06-2011
Publisher: Elsevier BV
Date: 10-2019
DOI: 10.1016/J.JENVMAN.2019.06.021
Abstract: Kerb side inlets with adjacent leaky wells are an emerging tool to harvest stormwater and to reduce runoff volumes and peak flow rates. This is achieved by collecting the first flush runoff into kerb side storages and infiltrating this water into the surrounding soil, thereby also reducing stormwater pollutant loadings. The hydraulic performance of the kerb side inlet, filter media and surrounding soil are key factors in the performance of these systems. However, no field or laboratory data are currently available for the hydraulic performance of a kerb side tree inlet pit. In this study, 12 tree inlet pits were constructed and filled with various media types including gravel, water treatment solids (a recycled waste product), sandy loam and clay to examine (1) leaky well infiltration rates (2) emptying times of the wells and (3) the well capacity (runoff storage volume) before and after runoff filtering through the wells. Using a laboratory model, the water harvesting performance of the kerb side inlet plate was also examined for various road longitudinal slopes. Using the field and laboratory data, simulation of the well performance was undertaken using the Model for Urban Stormwater Improvement Conceptualisation (MUSIC) to assess the capacity of these systems to reduce runoff volumes at the residential street scale. It was hypothesised that the type of filter media used in leaky well systems has a significant impact on the infiltration rate, regardless of the native soil type through which the stormwater eventually infiltrates. The results showed that the infiltration rates of systems filled with gravel were significantly higher than for the other media types, and this was followed by water treatment solids, sandy loam and clay. The results of the MUSIC modelling indicated that 2.8% of the mean annual runoff volume in the catchment could be harvested by the systems at the case study site. It was found that selection of high infiltration rate media and regular maintenance are the key factors for maintaining long-term performance of these systems.
Publisher: Springer Science and Business Media LLC
Date: 2013
Publisher: Institute of Mathematical Statistics
Date: 10-2010
DOI: 10.1214/09-AOS785
Publisher: Wiley
Date: 07-2017
DOI: 10.3835/PLANTGENOME2016.07.0064
Abstract: Early vigor is an important trait for many rice ( Oryza sativa L.)‐growing environments. However, genetic characterization and improvement for early vigor is hindered by the temporal nature of the trait and strong genotype × environment effects. We explored the genetic architecture of shoot growth dynamics during the early and active tillering stages by applying a functional modeling and genomewide association (GWAS) mapping approach on a ersity panel of ∼360 rice accessions. Multiple loci with small effects on shoot growth trajectory were identified, indicating a complex polygenic architecture. Natural variation for shoot growth dynamics was assessed in a subset of 31 accessions using RNA sequencing and hormone quantification. These analyses yielded a gibberellic acid (GA) catabolic gene, OsGA2ox7 , which could influence GA levels to regulate vigor in the early tillering stage. Given the complex genetic architecture of shoot growth dynamics, the potential of genomic selection (GS) for improving early vigor was explored using all 36,901 single‐nucleotide polymorphisms (SNPs) as well as several subsets of the most significant SNPs from GWAS. Shoot growth trajectories could be predicted with reasonable accuracy using the 50 most significant SNPs from GWAS (0.37–0.53) however, the accuracy of prediction was improved by including more markers, which indicates that GS may be an effective strategy for improving shoot growth dynamics during the vegetative growth stage. This study provides insights into the complex genetic architecture and molecular mechanisms underlying early shoot growth dynamics and provides a foundation for improving this complex trait in rice.
Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.SCITOTENV.2014.05.040
Abstract: Living roofs are an emerging green infrastructure technology that can potentially be used to ameliorate both climate change and urban heat island effects. There is not much information regarding the design of green roofs for dry climates and so the aim of this study was to develop low maintenance and unfertilized green roofs for a dry climate. This paper describes the effects of four important elements of green roofs namely slope, depth, growing media and plant species and their possible interactions in terms of plant growth responses in a dry climate. Sixteen medium-scale green roofs were set up and monitored during a one year period. This experiment consisted of twelve vegetated platforms and four non-vegetated platforms as controls. The design for the experiment was a split-split-plot design in which the factors Slope (1° and 25°) and Depth (100mm, 300 mm) were randomized to the platforms (main plots). Root depth and volume, average height of plants, final dry biomass and ground cover, relative growth rate, final dry shoot-root ratio, water use efficiency and leaf succulence were studied during a twelve month period. The results showed little growth of the plants in media type A, whilst the growth was significant in both media types B and C. On average, a 90% survival rate of plants was observed. Also the growth indices indicated that some plants can grow efficiently in the harsh environment created by green roofs in a dry climate. The root growth pattern showed that retained water in the drainage layer is an alternative source of water for plants. It was also shown that stormwater can be used as a source of irrigation water for green roofs during six months of the year at the study site. In summary, mild sloping intensive systems containing media type C and planted with either Chrysocephalum apiculatum or Disphyma crassifolium showed the best performance.
Publisher: Elsevier BV
Date: 06-1996
DOI: 10.1016/0268-0033(95)00072-0
Abstract: OBJECTIVE: To describe the effects of age and gender on three-dimensional (3D) active cervical spine motion. DESIGN: This was a descriptive study. BACKGROUND: This study expanded on previous investigations of age and gender effects on single plane motion of the cervical spine. METHODS: Sixty female and 60 male asymptomatic, normal volunteers, aged between 20 and 59 years, were examined in a standardized seated position. The 3 SPACE Isotrak system was used to measure simultaneous 3D motion of the cervical spine. RESULTS: The mean range of all the primary movements decreased significantly with age. For flexion/extension the greatest decrease occurred between the 20- and 30-year-olds, whereas for both lateral flexion and rotation, significant differences were demonstrated in subjects aged two decades apart. The coupling of motion associated with rotation was significantly related to age. CONCLUSION: Age had a significant effect on all of the primary movements. Age had less effect on the range of the coupled movements, in that the only movements to be affected were lateral flexion and extension occurring during cervical rotation. Gender had no marked effect on the primary or the coupled movements. RELEVANCE: This study provides normative data for the effects of age and gender on three-dimensional analysis of active cervical spine motion, which can be used for comparison with specific patient populations. The high level of intra-subject test-retest reliability renders the3 SPACE system of value for clinical measurement of movement pre- and post-treatment intervention for cervical spine disorders.
Publisher: Oxford University Press (OUP)
Date: 1984
Publisher: JSTOR
Date: 03-1983
DOI: 10.2307/2530806
Publisher: SAGE Publications
Date: 21-03-2012
Publisher: CSIRO Publishing
Date: 2021
DOI: 10.1071/FP20167
Abstract: Bread wheat (Triticum aestivum L.) is one of the most important food crops, however it is only moderately tolerant to salinity stress. To improve wheat yield under saline conditions, breeding for improved salinity tolerance of wheat is needed. We have identified nine quantitative trail loci (QTL) for different salt tolerance sub-traits in a recombinant inbred line (RIL) population, derived from the bi-parental cross of Excalibur × Kukri. This population was screened for salinity tolerance subtraits using a combination of both destructive and non-destructive phenotyping. Genotyping by sequencing (GBS) was used to construct a high-density genetic linkage map, consisting of 3236 markers, and utilised for mapping QTL. Of the nine mapped QTL, six were detected under salt stress, including QTL for maintenance of shoot growth under salinity (QG(1-5).asl-5A, QG(1-5).asl-7B) sodium accumulation (QNa.asl-2A), chloride accumulation (QCl.asl-2A, QCl.asl-3A) and potassium:sodium ratio (QK:Na.asl-2DS2). Potential candidate genes within these QTL intervals were shortlisted using bioinformatics tools. These findings are expected to facilitate the breeding of new salt tolerant wheat cultivars.
Publisher: Springer Science and Business Media LLC
Date: 22-04-2016
Publisher: Public Library of Science (PLoS)
Date: 09-11-2020
DOI: 10.1371/JOURNAL.PONE.0241966
Abstract: Drought and heat stress constrain wheat ( Triticum aestivum L.) yields globally. To identify putative mechanisms and candidate genes associated with combined drought and heat stress tolerance, we developed bread wheat near-isogenic lines (NILs) targeting a quantitative trait locus (QTL) on chromosome 6B which was previously associated with combined drought and heat stress tolerance in a erse panel of wheats. Genotyping-by-sequencing was used to identify additional regions that segregated in allelic pairs between the recurrent and the introduced exotic parent, genome-wide. NILs were phenotyped in a gravimetric platform with precision irrigation and exposed to either drought or to combined drought and heat stress from three days after anthesis. An increase in grain weight in NILs carrying the exotic allele at 6B locus was associated with thicker, greener leaves, higher photosynthetic capacity and increased water use index after re-watering. RNA sequencing of developing grains at early and later stages of treatment revealed 75 genes that were differentially expressed between NILs across both treatments and timepoints. Differentially expressed genes coincided with the targeted QTL on chromosome 6B and regions of genetic segregation on chromosomes 1B and 7A. Pathway enrichment analysis showed the involvement of these genes in cell and gene regulation, metabolism of amino acids and transport of carbohydrates. The majority of these genes have not been characterized previously under drought or heat stress and they might serve as candidate genes for improved abiotic stress tolerance.
Publisher: Wiley
Date: 22-02-2019
DOI: 10.1111/TPJ.14225
Publisher: Springer Science and Business Media LLC
Date: 11-10-2017
DOI: 10.1007/S10142-016-0526-8
Abstract: Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic Arabidopsis. We examined barley (Hordeum vulgare L. 'Golden Promise') plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing CaMV-35S::Ath-miR827 and drought-inducible Zm-Rab17::Hv-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUE
Publisher: Wiley
Date: 06-2019
DOI: 10.1111/ANZS.12260
Publisher: CSIRO Publishing
Date: 04-10-2021
DOI: 10.1071/FP21140
Abstract: Salinity tolerance in bread wheat is frequently reported to be associated with low leaf sodium (Na+) concentrations. However, the Portuguese landrace, Mocho de Espiga Branca, accumulates significantly higher leaf Na+ but has comparable salinity tolerance to commercial bread wheat cultivars. To determine the genetic loci associated with the salinity tolerance of this landrace, an F2 mapping population was developed by crossing Mocho de Espiga Branca with the Australian cultivar Gladius. The population was phenotyped for 19 salinity tolerance subtraits using both non-destructive and destructive techniques. Genotyping was performed using genotyping-by-sequencing (GBS). Genomic regions associated with salinity tolerance were detected on chromosomes 1A, 1D, 4B and 5A for the subtraits of relative and absolute growth rate (RGR, AGR respectively), and on chromosome 2A, 2B, 4D and 5D for Na+, potassium (K+) and chloride (Cl−) accumulation. Candidate genes that encode proteins associated with salinity tolerance were identified within the loci including Na+/H+ antiporters, K+ channels, H+-ATPase, calcineurin B-like proteins (CBLs), CBL-interacting protein kinases (CIPKs), calcium dependent protein kinases (CDPKs) and calcium-transporting ATPase. This study provides a new insight into the genetic control of salinity tolerance in a Na+ accumulating bread wheat to assist with the future development of salt tolerant cultivars.
Publisher: Informa UK Limited
Date: 2008
Publisher: Springer Science and Business Media LLC
Date: 09-2009
Publisher: Oxford University Press (OUP)
Date: 25-06-2015
DOI: 10.1104/PP.15.00450
Publisher: Oxford University Press (OUP)
Date: 19-07-2006
DOI: 10.1111/J.1467-9868.2006.00557.X
Abstract: Multitiered experiments are characterized by involving multiple randomizations, in a sense that we make explicit. We compare and contrast six types of multiple randomizations, using a wide range of ex les, and discuss their use in designing experiments. We outline a system of describing the randomizations in terms of sets of objects, their associated tiers and the factor nesting, using randomization diagrams, which give a convenient and readily assimilated summary of an experiment's randomization. We also indicate how to formulate a randomization-based mixed model for the analysis of data from such experiments.
Publisher: Springer Science and Business Media LLC
Date: 02-05-2017
DOI: 10.1038/S41598-017-01211-7
Abstract: Soil salinity results in reduced productivity in chickpea. However, breeding for salinity tolerance is challenging because of limited knowledge of the key traits affecting performance under elevated salt and the difficulty of high-throughput phenotyping for large, erse germplasm collections. This study utilised image-based phenotyping to study genetic variation in chickpea for salinity tolerance in 245 erse accessions. On average salinity reduced plant growth rate (obtained from tracking leaf expansion through time) by 20%, plant height by 15% and shoot biomass by 28%. Additionally, salinity induced pod abortion and inhibited pod filling, which consequently reduced seed number and seed yield by 16% and 32%, respectively. Importantly, moderate to strong correlation was observed for different traits measured between glasshouse and two field sites indicating that the glasshouse assays are relevant to field performance. Using image-based phenotyping, we measured plant growth rate under salinity and subsequently elucidated the role of shoot ion independent stress (resulting from hydraulic resistance and osmotic stress) in chickpea. Broad genetic variation for salinity tolerance was observed in the ersity panel with seed number being the major determinant for salinity tolerance measured as yield. This study proposes seed number as a selection trait in breeding salt tolerant chickpea cultivars.
Publisher: Elsevier BV
Date: 12-2020
Publisher: Figshare
Date: 2017
Publisher: Wiley
Date: 12-2017
DOI: 10.1111/ANZS.12221
Publisher: Elsevier BV
Date: 10-2021
Publisher: Institute of Mathematical Statistics
Date: 12-2009
DOI: 10.1214/09-AOS717
Publisher: Public Library of Science (PLoS)
Date: 07-10-2020
Publisher: Springer Science and Business Media LLC
Date: 08-09-2019
DOI: 10.1007/S11356-018-3083-Y
Abstract: The TREENET inlet is an emerging water-sensitive urban design technology that consists of a novel kerb side inlet coupled with a leaky well infiltration system. The inlets have been retrofitted to existing roads since 2006 however, there is currently little information available on the effectiveness of these inlet and leaky well systems. This study investigated the performance of the kerb side inlets and leaky well system for water quality improvement prior to infiltration to native soil. The leaky wells included four filter media types, namely gravel, water treatment solids, sandy loam and clay. To compare the performance of the four filter media types, batch and column studies were performed in the laboratory. The best performance was observed using the sandy loam as a filter media, followed by clay, water treatment solids and then gravel. The selection of effective media for removal of heavy metals is important as each media type has different pollutant removal capacity, infiltration and clogging performance.
Publisher: Wiley
Date: 14-10-2021
DOI: 10.1002/PPP3.10224
Abstract: Sorghum is an important cereal crop that provides calories and nutrients for much of the world's population, and it is often grown with low fertiliser input. Optimising the yield, nutritive content and bioavailability of sorghum grain with minimal input is of importance for human nutrition, and arbuscular mycorrhizal (AM) fungi have previously shown potential to assist in this. Across sorghum genetic ersity, AM fungi improved the yield, nutrition and zinc and iron bioavailability of grain in a low phosphorus soil. Thus, food production systems that effectively manage AM fungi may improve consumer outcomes. Sorghum is a C 4 cereal crop that is an important source of calories and nutrition across the world, predominantly cultivated and consumed in low‐ and middle‐income countries. Sorghum can be highly colonised by arbuscular mycorrhizal (AM) fungi, and the plant‐fungal association can lead to improvements in biomass and nutrient uptake. High‐throughput phenotyping allows us to non‐destructively interrogate the ‘hidden’ effects of AM fungi on sorghum growth and phenology. Eight genetically erse sorghum genotypes were grown in a soil amended with 2 or 20 mg P kg −1 and inoculated with an AM fungal culture of Rhizophagus irregularis . High‐throughput phenotyping uncovered the ‘hidden’ effects of AM fungi on growth and phenology, while grain biomass, nutrition, Zn and Fe bioavailability and root AM colonisation was determined after destructive harvest. Sorghum plants colonised by AM fungi generally performed better than non‐AM control plants, with greater yield, harvest indices, and grain P, Zn and Fe contents. During the early growth stages, AM colonisation led to temporary growth depressions. There were also AM fungal and P fertilisation effects on sorghum time‐of‐flowering. The sorghum genotype with the highest AM colonisation could barely produce grain when non‐inoculated. The two genotypes that failed to mature had very low AM colonisation. Generally, the genetically erse sorghum genotypes were highly responsive to AM colonisation and produced more grain of greater nutritive quality when colonised, without adverse consequences for micronutrient bioavailability.
Publisher: SAGE Publications
Date: 02-2022
DOI: 10.1177/09622802211031612
Abstract: In a recent paper, Jarrett, Farewell and Herzberg discussed a strategy for developing the analysis of a previously published two-phase experiment that investigated the effect of training on pain rating by occupational and physical therapy students. Here, their ex le is used to illustrate how a multi-step factor-allocation paradigm can be employed (i) to design an experiment, (ii) to understand the confounding in the design and (iii) to formulate linear mixed models, called prior allocation models, for the design. These models are intended as starting models for the analysis of the data, when it becomes available. An understanding of the confounding intrinsic to a design is achieved through an anatomy of the design presented in an analysis-of-variance-style table that can be obtained using functions from the [Formula: see text] package [Formula: see text]. The analysis of the pain-rating experiment is re-examined and it is recommended that conclusions be based on a model with heterogeneous residual variances, in addition to the previously proposed block-treatment interactions. The paradigm is also used in producing an alternative design, taking into account the results of the re-analysis.
Publisher: Springer Science and Business Media LLC
Date: 11-04-2019
Publisher: Wiley
Date: 04-08-2017
DOI: 10.1111/PCE.12764
Abstract: Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, 11 candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes, and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.
Publisher: Elsevier BV
Date: 10-1995
DOI: 10.1016/0268-0033(95)00010-I
Abstract: Isoresistive movements provide a functional method of testing muscle performance and of providing exercise the resistance applied is usually a percentage of the maximal isometric torque which can be developed by in idual subjects. The aim of the current study was to compare the effectiveness of dynamic isoresistive exercise programmes using 25, 50 or 75% of the maximum isometric flexion torque and static isometric exercises for improving the performance of the trunk flexors and extensors. Subjects comprised 42 asymptomatic females aged 18-25 years who were randomly assigned to control or exercise groups. The exercise groups undertook 2 min of exercise 3 times per week. The control subjects showed significant improvement between tests indicating a strong learning effect. The study showed that the subjects who exercised at 50% of maximum flexion isometric torque achieved the greatest increases in muscle performance on most measures. maximal isometric flexion and extension torques did not significantly change in any of the groups. Flexion and extension velocity and power were the muscle performance characteristics which improved the most in response to the training programmes. RELEVANCE:--This paper attempts to determine which among several options is the most effective isoresistive protocol for conditioning trunk muscle performance, as improved strength and power of these muscles may contribute in preventing episodes of LBP.
Publisher: Wiley
Date: 08-2019
DOI: 10.1111/ELE.13353
Abstract: Highly variable phenotypic responses in mycorrhizal plants challenge our functional understanding of plant-fungal mutualisms. Using non-invasive high-throughput phenotyping, we observed that arbuscular mycorrhizal (AM) fungi relieved phosphorus (P) limitation and enhanced growth of Brachypodium distachyon under P-limited conditions, while photosynthetic limitation under low nitrogen (N) was exacerbated by the fungus. However, these responses were strongly dependent on host genotype: only the faster growing genotype (Bd3-1) utilised P transferred from the fungus to achieve improved growth under P-limited conditions. Under low N, the slower growing genotype (Bd21) had a carbon and N surplus that was linked to a less negative growth response compared with the faster growing genotype. These responses were linked to the regulation of N : P stoichiometry, couples resource allocation to growth or luxury consumption in erse plant lineages. Our results attest strongly to a mechanism in plants by which plant genotype-specific resource economics drive phenotypic outcomes during AM symbioses.
Publisher: Wiley
Date: 03-04-2021
DOI: 10.1002/PPP3.10101
Abstract: Arbuscular mycorrhizal fungi (AMF) may contribute to enhanced yield and nutrition of host plants for the purpose of sustainable agriculture. However, the growth response of the host plant to mycorrhizal colonization is generally only measured at harvest, and thus management decisions regarding AMF are made using only a single time point. This study highlights that AMF can provide growth benefits to the host plant over its life. Greater knowledge of how plants respond to AMF over time will improve understanding of how the association functions and ultimately lead to improved management decisions regarding AMF in an agricultural context. Summary Colonization by arbuscular mycorrhizal fungi (AMF) can result in variable responses in the growth and mineral nutrition of host plants, and is highly dependent on soil nutrient condition limited studies have addressed the effects of AMF on plant growth over time. The aim of this study was to investigate the AMF effects on plant growth over the life of the plant, and interactions with soil phosphorus (P) and zinc (Zn) availability. We used a high‐throughput shoot phenotyping system to examine the temporal growth responses to AMF and soil P and Zn availabilities in the pasture legume Medicago truncatula . Plants were either inoculated with Rhizophagus irregularis or mock‐inoculated, and were examined under two soil P and five soil Zn availability treatments. Plants were then destructively harvested to obtain final biomass and shoot nutrition data. The growth of M. truncatula plants over time responded very differently to AMF depending on the soil P availability. At low P, projected shoot area and absolute growth rate (AGR) became increasingly greater in the mycorrhizal plants over the course of the experiment. At high P, there was a positive growth response to AMF until approximately 40 days after planting, after which the AGR of the non‐mycorrhizal plants increased and the response to AMF became neutral. Zinc availability was highly interactive with P availability, but not with AMF inoculation. This research demonstrates that growth responses to mycorrhizal fungi change over the plant's life, and are highly dependent on soil P availability.
Publisher: CSIRO Publishing
Date: 1977
DOI: 10.1071/EA9770871
Abstract: The development of the starwheel sprayer for the application of drying emulsion to Sultanas is described. This sprayer consists, in its standard form, of three sets of six radially and horizontally arranged spokes. Each spoke is fitted with three wide angle, solid cone nozzles which spray near and inside the vine canopy. Spraying vines with the starwheel sprayer took one tenth of the time needed to spray the fruit to saturation by hand. Experiments indicated that at least 4000 l ha-1 of spray emulsion containing at least 1.5 per cent oil and 1.875 per cent K2CO3 were needed to obtain sufficient coverage of the fruit for an adequate rate of drying and to ensure good quality of the fruit. The colour of dried sultanas produced by trellis drying after spraying with the starwheel sprayer was darker and less uniform than the colour of fruit produced by rack-spraying and -drying. This appeared to be related to the system of trellis-drying in general rather than to the use of the starwheel sprayer. It is concluded that the starwheel sprayer successfully meets the requirements for applying drying emulsion to Sultanas to be dried on the trellis.
Publisher: Wiley
Date: 09-1987
Publisher: Wiley
Date: 25-08-2020
DOI: 10.1002/CSC2.20257
Abstract: This paper is concerned with the generation of designs for early generation, plant‐breeding experiments that use limited experimental resources as efficiently as possible to maximize the realized genetic gain (RGG) resulting from the selection of lines. A number of authors have demonstrated that partially replicated ( p ‐rep) designs for such experiments, in which the percentage of lines that are duplicated is p , are likely to be more efficient than grid‐plot designs. Therefore, our aim is to obtain the most efficient p ‐rep design for an experiment using one of two distinctly different criteria and employing widely or readily available statistical software packages to search for an optimal design. However, this can be difficult because knowledge of the sources of variation and their magnitudes is required and is often unavailable. To overcome this impediment, a comprehensive simulation experiment was conducted to investigate whether designs that are robust to a wide range of experimental situations can be identified. Designs with p set to 20% and for different experimental situations are generated and the performance of each tested for 24 different variation scenarios. We concluded that for large experiments, the RGG obtained with various optimal designs is indeed not affected by the different variation scenarios and that resolved designs for fixed genetic effects should be generated for robustness. On the other hand, the design assumptions affect the RGG for small p ‐rep designs. Even so, an overall recommendation is made.
Publisher: Springer Science and Business Media LLC
Date: 30-07-2016
Publisher: Wiley
Date: 02-1975
Publisher: Springer Science and Business Media LLC
Date: 17-11-2016
DOI: 10.1038/NCOMMS13342
Abstract: High-throughput phenotyping produces multiple measurements over time, which require new methods of analyses that are flexible in their quantification of plant growth and transpiration, yet are computationally economic. Here we develop such analyses and apply this to a rice population genotyped with a 700k SNP high-density array. Two rice ersity panels, indica and aus , containing a total of 553 genotypes, are phenotyped in waterlogged conditions. Using cubic smoothing splines to estimate plant growth and transpiration, we identify four time intervals that characterize the early responses of rice to salinity. Relative growth rate, transpiration rate and transpiration use efficiency (TUE) are analysed using a new association model that takes into account the interaction between treatment (control and salt) and genetic marker. This model allows the identification of previously undetected loci affecting TUE on chromosome 11, providing insights into the early responses of rice to salinity, in particular into the effects of salinity on plant growth and transpiration.
Publisher: Cold Spring Harbor Laboratory
Date: 19-07-2018
DOI: 10.1101/372516
Abstract: Nepotism and reciprocity are not mutually exclusive explanations for cooperation, because helping decisions can depend on both kinship cues and past reciprocal help. The importance of these two factors can therefore be difficult to disentangle using observational data. We developed a res ling procedure for inferring the statistical power to detect observational evidence of nepotism and reciprocity. We first applied this procedure to simulated datasets resulting from perfect reciprocity, where the probability and duration of helping events from in idual A to B equaled that from B to A. We then assessed how the probability of detecting correlational evidence of reciprocity was influenced by (1) the number of helping observations and (2) varying degrees of simultaneous nepotism. Last, we applied the same analysis to empirical data on food sharing in v ire bats and allogrooming in mandrills and Japanese macaques. We show that at smaller s le sizes, the effect of kinship was easier to detect and the relative role of kinship was overestimated compared to the effect of reciprocal help in both simulated and empirical data, even with data simulating perfect reciprocity and imperfect nepotism. We explain the causes and consequences of this difference in power for detecting the roles of kinship versus reciprocal help. To compare the relative importance of genetic and social relationships, we therefore suggest that researchers measure the relative reliability of both coefficients in the model by plotting these coefficients and their detection probability as a function of s ling effort. We provide R scripts to allow others to do this power analysis with their own datasets.
Publisher: Springer Science and Business Media LLC
Date: 30-07-2018
DOI: 10.1007/S00122-018-3146-Y
Abstract: Novel QTL for salinity tolerance traits have been detected using non-destructive and destructive phenotyping in bread wheat and were shown to be linked to improvements in yield in saline fields. Soil salinity is a major limitation to cereal production. Breeding new salt-tolerant cultivars has the potential to improve cereal crop yields. In this study, a doubled haploid bread wheat mapping population, derived from the bi-parental cross of Excalibur × Kukri, was grown in a glasshouse under control and salinity treatments and evaluated using high-throughput non-destructive imaging technology. Quantitative trait locus (QTL) analysis of this population detected multiple QTL under salt and control treatments. Of these, six QTL were detected in the salt treatment including one for maintenance of shoot growth under salinity ( QG (1 – 5) .asl - 7A ), one for leaf Na + exclusion ( QNa.asl - 7A ) and four for leaf K + accumulation ( QK.asl - 2B.1 , QK.asl - 2B.2 , QK.asl - 5A and QK:Na.asl - 6A ). The beneficial allele for QG (1 – 5) .asl - 7A (the maintenance of shoot growth under salinity) was present in six out of 44 mainly Australian bread and durum wheat cultivars. The effect of each QTL allele on grain yield was tested in a range of salinity concentrations at three field sites across 2 years. In six out of nine field trials with different levels of salinity stress, lines with alleles for Na + exclusion and/or K + maintenance at three QTL ( QNa.asl - 7A , QK.asl - 2B.2 and QK:Na.asl - 6A ) excluded more Na + or accumulated more K + compared to lines without these alleles. Importantly, the QK.asl - 2B.2 allele for higher K + accumulation was found to be associated with higher grain yield at all field sites. Several alleles at other QTL were associated with higher grain yields at selected field sites.
Publisher: CSIRO Publishing
Date: 1978
DOI: 10.1071/EA9780301
Abstract: Pruning Sultana vines in the Murray Valley by the system of 'arched canes' is described. In two experiments there were trends towards slight increases in crop despite smaller bunch weight when the canes were arched downwards instead of wrapped tightly around thetrellis wire. Downward arching did not greatly affect overall vine performance, but it modified the distribution of growth and yield along the canes, leading to more bunches on the middle part of the cane and shorter shoots on the distal part of the cane. Arching the canes upwards gave similar results to wrapping and showed no commercial promise. Harvest pruning was somewhat faster when the canes were arched downwards, but there was no quality difference in the trellis dried fruit. Winter pruning was speeded up by up to one-half of normal pruning time when the canes were arched, particularly when clips were used to attach them to the trellis wire. Vines pruned by arching had sufficient replacement canes although they produced fewer mature shoots near their crowns than vines pruned by wrapping. It is concluded that pruning Sultana vines by arching canes downwards leads to several beneficial changes, i.e. reduced bunch weight without loss of crop, less fruit clumping where the canes of two adjacent vines meet, shorter terminal shoots, and reduced pruning time. This can be achieved without major changes of the trellis.
Publisher: Cold Spring Harbor Laboratory
Date: 24-01-2020
DOI: 10.1101/2020.01.21.909887
Abstract: Improving salinity tolerance in the most widely cultivated cereal, bread wheat ( Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up to 6 folds greater leaf and sheath sodium (Na + ) than two Australian cultivars, Gladius and Scout, under salt stress. Despite high leaf and sheath Na + concentrations, Mocho de Espiga Branca maintained similar salinity tolerance compared to Gladius and Scout. A naturally occurring single nucleotide substitution was identified in the gene encoding a major Na + transporter TaHKT1 -D in Mocho de Espiga Branca, which resulted in a L190P amino acid residue variation. This variant prevents Mocho de Espiga Branca from retrieving Na + from the root xylem leading to a high shoot Na + concentration. The identification of the tissue tolerant Mocho de Espiga Branca will accelerate the development of more elite salt tolerant bread wheat cultivars.
Publisher: Wiley
Date: 15-11-2012
DOI: 10.1002/ASI.22742
No related grants have been discovered for Chris Brien.