ORCID Profile
0000-0003-2485-3457
Current Organisation
University of South Australia
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Publisher: Wiley
Date: 18-04-2023
DOI: 10.1002/YEA.3851
Abstract: Beer refermentation in bottles is an industrial process utilized by breweries where yeast and fermentable extract are added to green beer. The beer is refermented for a minimum of 2 weeks before distribution, with the physiological state of the yeast a critical factor for successful refermentation. Ideally, fresh yeast that is propagated from a dedicated propagation plant should be used for refermentation in bottles. Here, we explored the applicability of the fluorescent and redox‐sensitive dye, resazurin, to assess cellular metabolism in yeast and its ability to differentiate between growth stages. We applied this assay, with other markers of yeast physiology, to evaluate yeast quality during a full‐scale industrial propagation. Resazurin allowed the discrimination between the different growth phases in yeast and afforded a more in‐depth understanding of yeast metabolism during propagation. This assay can be used to optimize the yeast propagation process and cropping time to improve beer quality.
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C8RA06188B
Abstract: The preparation of polymerised high internal phase emulsions with high shear in narrower capillary dimensions results in significant improvements in the chromatographic performance for the separation of proteins.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1RA07705H
Abstract: Poly(styrene- co - inylbenzene)-based monoliths prepared from the polymerisation of water-in-monomer high internal phase emulsions, where water-soluble monomers acrylamide or poly(ethylene glycol) diacrylate ( M w 258) were included in internal phase.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C5PY02012C
Abstract: Oil-in-water (‘inverse’) High Internal Phase Emulsions (HIPEs) have been prepared using an hiphilic macro-RAFT agent with toluene as the internal dispersed phase (∼80 vol%) and an aqueous monomer solution as the continuous phase.
Publisher: Elsevier BV
Date: 09-2021
Publisher: MDPI AG
Date: 20-04-2023
DOI: 10.3390/CIMB45040235
Abstract: Nearly 90% of cervical cancers are linked to human papillomavirus (HPV). Uncovering the protein signatures in each histological phase of cervical oncogenesis provides a path to biomarker discovery. The proteomes extracted from formalin-fixed paraffin-embedded tissues of the normal cervix, HPV16/18-associated squamous intraepithelial lesion (SIL), and squamous cell carcinoma (SCC) were compared using liquid chromatography-mass spectrometry (LC-MS). A total of 3597 proteins were identified, with 589, 550, and 1570 proteins unique to the normal cervix, SIL, and SCC groups, respectively, while 332 proteins overlapped between the three groups. In the transition from normal cervix to SIL, all 39 differentially expressed proteins were downregulated, while all 51 proteins discovered were upregulated in SIL to SCC. The binding process was the top molecular function, while chromatin silencing in the SIL vs. normal group, and nucleosome assembly in SCC vs. SIL groups was the top biological process. The PI3 kinase pathway appears crucial in initiating neoplastic transformation, while viral carcinogenesis and necroptosis are important for cell proliferation, migration, and metastasis in cervical cancer development. Annexin A2 and cornulin were selected for validation based on LC-MS results. The former was downregulated in the SIL vs. normal cervix and upregulated in the progression from SIL to SCC. In contrast, cornulin exhibited the highest expression in the normal cervix and lowest in SCC. Although other proteins, such as histones, collagen, and vimentin, were differentially expressed, their ubiquitous expression in most cells precluded further analysis. Immunohistochemical analysis of tissue microarrays found no significant difference in Annexin A2 expression between the groups. Conversely, cornulin exhibited the strongest expression in the normal cervix and lowest in SCC, supporting its role as a tumor suppressor and potential biomarker for disease progression.
Publisher: American Chemical Society (ACS)
Date: 26-01-2021
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8GC01055B
Abstract: Preparation of polymer monoliths from the coagulation of particles, synthesized by soap-free emulsion polymerisation, using water as the only solvent.
Publisher: Elsevier BV
Date: 05-2018
Publisher: Elsevier BV
Date: 02-2023
Publisher: American Chemical Society (ACS)
Date: 17-09-2018
DOI: 10.1021/ACS.ANALCHEM.8B02893
Abstract: Multimaterial 3D printing facilitates the rapid production of complex devices with integrated materials of varying properties and functionality. Herein, multimaterial fused deposition modeling (MM-FDM) 3D printing was applied to the fabrication of low-cost passive s ler devices with integrated porous membranes. Using MM-FDM 3D printing, the device body was produced using black polylactic acid, with Poro-Lay Lay-Felt filament used for the printing of the integrated porous membranes (rubber-elastomeric polymer, porous after removal of a water-soluble poly(vinyl alcohol) component). The resulting device consisted of two interlocking circular frames, each containing the integrated membrane, which could be efficiently sealed together without the need for additional O-rings, and prevented loss of enclosed microparticulate sorbent. Scanning electron microscopy (SEM) analysis of the purified composite filament confirmed the porous properties of the material, an average pore size of ∼30 nm. The printed passive s lers with various membrane thicknesses, including 0.5, 1.0, and 1.5 mm, were evaluated for their ability to facilitate the extraction of atrazine as the model solute onto the internal sorbent, under standard conditions. Gas chromatography-mass spectrometry was used to determine the uptake of atrazine by the device from standard water s les and also to evaluate any chemical leaching from the printed materials. The s ler with 0.5 mm thick membrane showed the best performance with 87% depletion and a s ling rate of 0.19 Ld
Publisher: Wiley
Date: 16-03-2021
Abstract: The applicability of mass spectrometry imaging (MSI) has exponentially increased with the improvement of s le preparation, instrumentation (spatial resolution) and data analysis. The number of MSI publications listed in PubMed continues to grow with 378 published articles in 2020‐2021. Initially, MSI was just sensitive enough to identify molecular features correlating with distinct tissue regions, similar to the resolution achieved by visual inspection after standard immunohistochemical staining. Although the spatial resolution was limited compared with other imaging modalities, the molecular intensity mapping added a new exciting capability. Over the past decade, significant improvements in every step of the workflow and most importantly in instrumentation were made, which now enables the molecular analysis at a cellular and even subcellular level. Here, we summarize the latest developments in MSI, with a focus on the latest approaches for tissue‐based imaging described in 2020.
Publisher: Elsevier BV
Date: 03-2024
No related grants have been discovered for Christopher Desire.