ORCID Profile
0000-0002-9999-9154
Current Organisation
University of South Australia
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Biochemistry and Cell Biology | Organic Chemical Synthesis | Manufacturing Processes and Technologies (excl. Textiles) | Materials Engineering | Physical Chemistry (Incl. Structural) | Composite and Hybrid Materials | Structural Chemistry and Spectroscopy | Analytical Biochemistry | Structural Biology (incl. Macromolecular Modelling) | Systems Biology |
ANZSRC Socio-Economic Objective (SEO)
Rehabilitation of Degraded Fresh, Ground and Surface Water Environments | Human Pharmaceutical Treatments (e.g. Antibiotics) | Beverages (excl. Fruit Juices) | Expanding Knowledge in the Chemical Sciences | Expanding Knowledge in the Agricultural and Veterinary Sciences | Expanding Knowledge in the Biological Sciences | Environmentally Sustainable Manufacturing not elsewhere classified | National Security | Environmental Health
Related Links
Publications
The effect on the lanthanide luminescence of structurally simple Eu(iii) cyclen complexes upon deprotonation of metal bound water molecules and amide based pendant arms
Publisher: Royal Society of Chemistry (RSC)
Date: 2010
DOI: 10.1039/B923383K
Abstract: A series of substituted 1,4,7,10-tetraazacylcododecane ligands 1-4, possessing sensitizing nitrobenzene or naphthalene antennae, as one of the amide pendant arms, and their complexes with europium(III) were synthesised. The protonation constants and the metal ion stability constants of two of these ligands were determined by potentiometric titration. The pK(a) of the water molecules coordinated to the complexed metal ion were determined by both luminescent and potentiometric measurements. The luminescence pH dependence of a further three Eu(III) complexes, 5-7, which lack any antennae, were also studied with the aim of gaining a better understanding of the role of the metal bound water molecules in the luminescence properties of such complexes upon direct excitation of the lanthanide ion. The results from these luminescent measurements demonstrate that the Eu(III) emission was significantly modulated as a function of pH for all the complexes, which we assigned to changes occurring in the coordination environment of the ion within the cyclen system, caused by deprotonation of metal bound water molecules and/or deprotonation of pendent amide arms.
Development of an optical fiber‐based redox monitoring system for tissue metabolism
Publisher: Wiley
Date: 25-01-2022
Abstract: An optical redox ratio can potentially be used to report on the dynamics of cell and tissue metabolism and define altered metabolic conditions for different pathologies. While there are methods to measure the optical redox ratio, they are not particularly suited to real‐time in situ or in vivo analysis. Here, we have developed a fiber‐optic system to measure redox ratios in cells and tissues and two mathematical models to enable real‐time, in vivo redox measurements. The optical redox ratios in tissue explants are correlated directly with endogenous NADH/FAD fluorescence emissions. We apply the mathematical models to the two‐photon microscopy data and show consistent results. We also used our fiber‐optic system to measure redox in different tissues and show consistent results between the two models, hence demonstrating proof‐of‐principle. This innovative redox monitoring system will have practical applications for defining different metabolic disease states.
Singlet Oxygen Detection on a Nanostructured Porous Silicon Thin Film via Photonic Luminescence Enhancements
Publisher: American Chemical Society (ACS)
Date: 20-04-2017
DOI: 10.1021/ACS.LANGMUIR.7B00522
Abstract: Because reactive oxygen species are involved in a range of pathologies, developing analytical tools for this group of molecules opens new vistas for biomedical diagnostics. Herein, we fabricate a porous silicon microcavity (pSiMC) functionalized with luminescent singlet oxygen (
Fluorescent ligands derived from 2-(9-anthrylmethylamino)ethyl-appended cyclen for use in metal ion activated molecular receptors
Publisher: Elsevier BV
Date: 07-2009
Norbornane-based cationic antimicrobial peptidomimetics targeting the bacterial membrane
Publisher: Elsevier BV
Date: 12-2018
Selective mono N-alkylations of cyclen in one step syntheses
Publisher: Elsevier BV
Date: 11-2007
Novel Gd-Loaded Silicon Nanohybrid: A Potential Epidermal Growth Factor Receptor Expressing Cancer Cell Targeting Magnetic Resonance Imaging Contrast Agent
Publisher: American Chemical Society (ACS)
Date: 12-2017
Abstract: Continuing our research efforts in developing mesoporous silicon nanoparticle-based biomaterials for cancer therapy, we employed here porous silicon nanoparticles as a nanocarrier to deliver contrast agents to diseased cells. Nanoconfinement of small molecule Gd-chelates (L1-Gd) enhanced the T
Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time
Publisher: Wiley
Date: 09-2016
Abstract: Live cell imaging can provide important information on cellular dynamics however, the full utilisation of this technology has been h ered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3 (1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER(™) , shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER(™) localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.
Surface Functionalised Optical Fibre for Detection of Hydrogen Sulphide
Publisher: MDPI AG
Date: 24-10-2023
DOI: 10.3390/BIOS13110949
Crosslinked shells for nano-assembled capsules: a new encapsulation method for smaller Gd3+-loaded capsules with exceedingly high relaxivities
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7CC00123A
Abstract: Nano-capsules with robust, covalently bound, crosslinked shells can be used to encapsulate large payloads of MRI contrast agent, such as GdDOTP 5− , that exhibit very high per-Gd 3+ relaxivities.
Interactions of proximate amino acid residues in polyaza macrocyclic systems
Publisher: Elsevier BV
Date: 07-2003
Imaging and lipidomics methods for lipid analysis in metabolic and cardiovascular disease
Publisher: Cambridge University Press (CUP)
Date: 12-07-2017
DOI: 10.1017/S2040174417000496
Abstract: Cardiometabolic diseases exhibit changes in lipid biology, which is important as lipids have critical roles in membrane architecture, signalling, hormone synthesis, homoeostasis and metabolism. However, Developmental Origins of Health and Disease studies of cardiometabolic disease rarely include analysis of lipids. This short review highlights some ex les of lipid pathology and then explores the technology available for analysing lipids, focussing on the need to develop imaging modalities for intracellular lipids. Analytical methods for studying interactions between the complex endocrine and intracellular signalling pathways that regulate lipid metabolism have been critical in expanding our understanding of how cardiometabolic diseases develop in association with obesity and dietary factors. Biochemical methods can be used to generate detailed lipid profiles to establish links between lifestyle factors and metabolic signalling pathways and determine how changes in specific lipid subtypes in plasma and homogenized tissue are associated with disease progression. New imaging modalities enable the specific visualization of intracellular lipid traffic and distribution in situ . These techniques provide a dynamic picture of the interactions between lipid storage, mobilization and signalling, which operate during normal cell function and are altered in many important diseases. The development of methods for imaging intracellular lipids can provide a dynamic real-time picture of how lipids are involved in complex signalling and other cell biology pathways and how they ultimately regulate metabolic function/homoeostasis during early development. Some imaging modalities have the potential to be adapted for in vivo applications, and may enable the direct visualization of progression of pathogenesis of cardiometabolic disease after poor growth in early life.
Tuning the properties of cyclen based lanthanide complexes for phosphodiester hydrolysis; the role of basic cofactors
Publisher: Royal Society of Chemistry (RSC)
Date: 2006
DOI: 10.1039/B609923H
Abstract: The synthesis of several cyclen based lanthanide [Eu(III) and La(III)] complexes is described these metallo ribonuclease mimics are based on the use of alkyl amines as pendent arms, which give rise to fast hydrolysis within the physiological pH range of HPNP (an RNA model compound) that is highly dependent on the length of the alkyl spacer.
Recent Advances on Luminescent Enhancement-Based Porous Silicon Biosensors
Publisher: Springer Science and Business Media LLC
Date: 25-02-2016
DOI: 10.1007/S11095-016-1889-1
Abstract: Luminescence-based detection paradigms have key advantages over other optical platforms such as absorbance, reflectance or interferometric based detection. However, autofluorescence, low quantum yield and lack of photostability of the fluorophore or emitting molecule are still performance-limiting factors. Recent research has shown the need for enhanced luminescence-based detection to overcome these drawbacks while at the same time improving the sensitivity, selectivity and reducing the detection limits of optical sensors and biosensors. Nanostructures have been reported to significantly improve the spectral properties of the emitting molecules. These structures offer unique electrical, optic and magnetic properties which may be used to tailor the surrounding electrical field of the emitter. Here, the main principles behind luminescence and luminescence enhancement-based detections are reviewed, with an emphasis on europium complexes as the emitting molecule. An overview of the optical porous silicon microcavity (pSiMC) as a biosensing platform and recent proof-of-concept ex les on enhanced luminescence-based detection using pSiMCs are provided and discussed.
Soft Matter pH Sensing: From Luminescent Lanthanide pH Switches in Solution to Sensing in Hydrogels
Publisher: American Chemical Society (ACS)
Date: 08-2006
DOI: 10.1021/CM060603V
Lanthanide luminescent anion sensing: evidence of multiple anion recognition through hydrogen bonding and metal ion coordination
Publisher: Royal Society of Chemistry (RSC)
Date: 2007
DOI: 10.1039/B705560A
Abstract: The delayed lanthanide luminescence of the terbium [Tb(III)] diaryl-urea complex 1xTb is significantly enhanced upon sensing of dihydrogenphosphate (H2PO4(-)) in CH3CN, which occurs through multiple anion binding through hydrogen bonding interactions and potential metal ion coordination to Tb(III).
Label-free imaging of healthy and infarcted fetal sheep hearts by two-photon microscopy
Publisher: Wiley
Date: 02-05-2018
Abstract: Coronary heart disease is one of the largest causes of death worldwide, making this a significant health care issue. A critical problem for the adult human heart is that it does not undergo effective repair in response to damage, leaving patients with a poor prognosis. Unlike the adult, fetal hearts have the ability to repair after myocardial damage. Using two-photon microscopy, we have visualised the morphological and metabolic changes following myocardial infarction in sheep fetuses, to characterise response to cardiac injury in a mammalian model. Following myocardial infarction, fetal hearts showed no significant increase in collagen deposition in the region of the infarction, when compared to either the surrounding tissue or shams. In contrast, metabolic activity (i. e. NAD(P)H and FAD) was significantly reduced in the region of myocardial infarction, when compared to either the surrounding tissue or sham hearts. For comparison, we also imaged two hearts from preadolescent sheep (sham and myocardial infarction) and showed highly ordered collagen deposition with decreased metabolic activity within the infarcted area. Therefore, two-photon imaging had the capacity to image both morphological and metabolic changes in response to myocardial infarction and showed differences in the response with age. Picture: Two-photon imaging of myocardial infarction (b and d) enabled the visualisation of increased collagen (blue Em=431 nm) and changes in other tissue autofluorescence (green Em=489-606 nm) in fetal (a and b) and preadolescent (c and d) hearts, compared to shams (a and c). The excitation wavelength was 840 nm. Scale bars: 10 μm.
Lanthanide Luminescence Enhancements in Porous Silicon Resonant Microcavities
Publisher: American Chemical Society (ACS)
Date: 28-07-2014
DOI: 10.1021/AM500983R
Abstract: In this paper, the covalent immobilization and luminescence enhancement of a europium (Eu(III)) complex in a porous silicon (pSi) layer with a microcavity (pSiMC) structure are demonstrated. The alkyne-pendant arm of the Eu(III) complex was covalently immobilized on the azide-modified surface via ligand-assisted "click" chemistry. The design parameters of the microcavity were optimized to obtain an efficient luminescence-enhancing device. Luminescence enhancements by a factor of 9.5 and 3.0 were observed for Eu(III) complex bound inside the pSiMC as compared to a single layer and Bragg reflector of identical thickness, respectively, confirming the increased interaction between the immobilized molecules and the electric field in the spacer of the microcavity. When comparing pSiMCs with different resonance wavelength position, luminescence was enhanced when the resonance wavelength overlapped with the maximum emission wavelength of the Eu(III) complex at 614 nm, allowing for effective coupling between the confined light and the emitting molecules. The pSiMC also improved the spectral color purity of the Eu(III) complex luminescence. The ability of a pSiMC to act as an efficient Eu(III) luminescence enhancer, combined with the resulting sharp linelike emission, can be exploited for the development of ultrasensitive optical biosensors.
Label‐free imaging of redox status and collagen deposition showing metabolic differences in the heart
Publisher: Wiley
Date: 28-11-2018
Abstract: The heart has high metabolic demand to maintain function. The primary source of energy supply to support correct contractile muscle function differs between a fetus and an adult. In fetal life, ATP is primarily generated by glycolysis and lactate oxidation, whereas following birth, there is a shift towards a reliance on mitochondrial metabolism and fatty acid oxidation. This change in metabolic status is an adaptation to different fuel availability, oxygenation and growth patterns. In this study, we have employed 2-photon excitation fluorescence microscopy to define the relationship between two critical metabolic cofactors nicotinamide adenine dinucleotide(P)H and flavin adenine dinucleotide, effectively utilizing a redox ratio to differentiate between the metabolic status in fetal (proliferative) and adult (quiescent/hypertrophic) hearts. Two-photon imaging was also used to visually confirm the known increase in collagen deposition in the adult heart. The changes observed were consistent with a hypertrophic growth profile and greater availability of fatty acids in the adult heart, compared to the proliferative fetal heart. Two-photon excitation fluorescence microscopy is therefore a convenient imaging technology that enables the monitoring of striated muscle architecture and the metabolic status of heart tissue. This imaging technology can potentially be employed to visualize cardiac and other muscle pathologies.
Solution studies of trimetallic lanthanide luminescent anion sensors: Towards ratiometric sensing using an internal reference channel
Publisher: Royal Society of Chemistry (RSC)
Date: 2008
DOI: 10.1039/B805610B
Abstract: Luminescent europium and terbium complexes and a mixed Eu(III)-Tb(III) complex were prepared, each with three macrocycles coordinating to a single lanthanide ion to form a trimetallic system, and can be used for the ratiometric sensing of anions in the case of the mixed complex.
Luminescent Sensing of Dicarboxylates in Water by a Bismacrocyclic Dinuclear Eu(III) Conjugate
Publisher: American Chemical Society (ACS)
Date: 21-04-2007
DOI: 10.1021/OL070339R
Abstract: The design, synthesis, and characterization of a novel dinuclear Eu(III) bismacrocyclic conjugate 1.Eu2 as a delayed luminescent lanthanide sensor for dicarboxylates is discussed. The sensor was shown to bind small dicarboxylic acids such as aspartic, malonic, succinic, or glutaric acid in pH 6.5 solutions. However, only malonic acid gave rise to selective Eu(III) luminescent enhancements, as the emission was reduced for all of the other acids.
Modulation of the organelle specificity in Re(i ) tetrazolato complexes leads to labeling of lipid droplets
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4RA00050A
Abstract: Neutral Re( i ) tetrazolato complexes exhibit labeling of lipid droplets with high specificity.
A Molecular Probe for the Detection of Polar Lipids in Live Cells
Publisher: Public Library of Science (PLoS)
Date: 23-08-2016
Spectroscopic and molecular docking study of the interaction between neutral Re(I) tetrazolate complexes and bovine serum albumin.
Publisher: Wiley
Date: 17-06-2021
Abstract: Re(I) complexes have potential in biomedical sciences as imaging agents, diagnostics and therapeutics. Thus, it is crucial to understand how Re(I) complexes interact with carrier proteins, like serum albumins. Here, two neutral Re(I) complexes were used ( fac ‐[Re(CO) 3 (1,10‐phenanthroline)L], in which L is either 4‐cyanophenyltetrazolate (1) or 4‐methoxycarbonylphenyltetrazole ester (2) , to study the interactions with bovine serum albumin (BSA). Spectroscopic measurements, calculations of thermodynamic and Förster resonance energy transfer parameters, as well as molecular modelling, were performed to study differential binding between BSA and complex 1 and 2 . Induced‐fit docking combined with quantum‐polarised ligand docking were employed in what is believed to be a first for a Re(I) complex as a ligand for BSA. Our findings provide a basis for other molecular interaction studies and suggest that subtle functional group alterations at the terminal region of the Re(I) complex have a significant impact on the ability of this class of compounds to interact with BSA.
ARID5B as a critical downstream target of the TAL1 complex that activates the oncogenic transcriptional program and promotes T-cell leukemogenesis
Publisher: Cold Spring Harbor Laboratory
Date: 12-2017
Abstract: The oncogenic transcription factor TAL1/SCL induces an aberrant transcriptional program in T-cell acute lymphoblastic leukemia (T-ALL) cells. However, the critical factors that are directly activated by TAL1 and contribute to T-ALL pathogenesis are largely unknown. Here, we identified AT-rich interactive domain 5B ( ARID5B ) as a collaborating oncogenic factor involved in the transcriptional program in T-ALL. ARID5B expression is down-regulated at the double-negative 2–4 stages in normal thymocytes, while it is induced by the TAL1 complex in human T-ALL cells. The enhancer located 135 kb upstream of the ARID5B gene locus is activated under a superenhancer in T-ALL cells but not in normal T cells. Notably, ARID5B-bound regions are associated predominantly with active transcription. ARID5B and TAL1 frequently co-occupy target genes and coordinately control their expression. ARID5B positively regulates the expression of TAL1 and its regulatory partners. ARID5B also activates the expression of the oncogene MYC . Importantly, ARID5B is required for the survival and growth of T-ALL cells , and forced expression of ARID5B in immature thymocytes results in thymus retention, differentiation arrest, radioresistance, and tumor formation in zebrafish. Our results indicate that ARID5B reinforces the oncogenic transcriptional program by positively regulating the TAL1-induced regulatory circuit and MYC in T-ALL, thereby contributing to T-cell leukemogenesis.
Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissues
Publisher: Wiley
Date: 23-12-2020
Abstract: The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two-photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism.
Synthesis and Characterisation of First Generation Luminescent Lanthanide Complexes Suitable for Being Adapted for Uptake via the Mannose Receptor
Publisher: Hindawi Limited
Date: 18-12-2013
DOI: 10.1155/2013/498598
Abstract: With the aim of directing lanthanide complex uptake via the mannose receptor, a first generation of luminescent lanthanide complexes has been developed with an α -D-mannose targeting motif. Four complexes were produced to investigate photophysical properties and determine the effect of the coordinated mannose residue on emission intensity. The free hydroxyls of the α -D-mannose residue quenched lanthanide phosphorescence due to their close proximity, though they did not bind the lanthanide centre as observed by q -values ≈ 1.0 for all complexes between pH 3 and 10. Fluorescent emission was found to vary significantly with pH, though phosphorescent emission was relatively insensitive to pH. This lack of pH sensitivity has the potential to provide stable emission for the visualisation of the endosome-lysosome system where acidic pH is often encountered.
Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6MB00242K
Abstract: Localisation of a neutral rhenium( i ) tricarbonyl phenanthroline species to regions of high polar lipid concentrations is demonstrated by Fourier transform infrared (FTIR) microspectroscopy.
A europium-based ‘off-on’ colourimetric detector of singlet oxygen
Publisher: Elsevier BV
Date: 06-2017
A Dinuclear Lanthanide Complex for the Recognition of Bis(carboxylates): Formation of Terbium(III) Luminescent Self-Assembly Ternary Complexes in Aqueous Solution
Publisher: American Chemical Society (ACS)
Date: 18-10-2006
DOI: 10.1021/IC0614418
Abstract: The synthesis and photophysical properties of a coordinatively unsaturated cationic dinuclear terbium complex, 2.Tb(2), that can detect the presence of mono- or bis(carboxylates) in buffered aqueous solution at physiological pH is described. Full ligand synthesis and structural characterization of 2.Na(2) are also described. Spectroscopic measurements determined that each Tb(III) metal center has two metal-bound water molecules (q = 2). The recognition or sensing of N,N-dimethylaminocarboxylic acid, 4, and the bis(carboxylate) terephthalic acid, 5, which can also function as sensitizing antennae, was found to occur through the binding of these carboxylates to the metal center via the displacement of the metal bound water molecules. This gave rise to the formation of luminescent ternary complexes in solution in 2:1 or 1:1 (ion:2.Tb(2)) stoichiometry, respectively. Aliphatic bis(carboxylates) also bind to 2.Tb(2) where the selectivity for the ion recognition and stoichiometry was dictated by the structure of the anion, being most selective for pimelic acid, 6. Binding of either l- or d-tartaric acid gave rise to the formation ternary complex formation, with 2:1 stoichiometry, where the ion recognition resulted in quenching of the lanthanide emission.
Supramolecular Self-Assembly of Mixed f−d Metal Ion Conjugates
Publisher: American Chemical Society (ACS)
Date: 23-05-2006
DOI: 10.1021/OL060752J
Abstract: [reaction: see text] The synthesis of the novel Eu(III) cyclen complex, Eu1, is described. In buffered pH 7.4 water, the Eu(III) emission was "switched on" upon excitation of the Terpy antenna. In the presence of various transition-metal ions (e.g., Fe(II) and Ni(II)), both the singlet-excited state and the Eu(III) emission were quenched ("switched off"), whereas in the ground state, an MLCT band was formed, signifying the formation of stable mixed linear trimetallic f-d supramolecular self-assemblies.
Redox ratio in the left ventricle of the growth restricted fetus is positively correlated with cardiac output
Publisher: Wiley
Date: 16-09-2021
Abstract: Intrauterine growth restriction (IUGR) is a result of limited substrate supply to the developing fetus in utero, and can be caused by either placental, genetic or environmental factors. Babies born IUGR can have poor long‐term health outcomes, including being at higher risk of developing cardiovascular disease. Limited substrate supply in the IUGR fetus not only changes the structure of the heart but may also affect metabolism and function of the developing heart. We have utilised two imaging modalities, two‐photon microscopy and phase‐contrast MRI (PC‐MRI), to assess alterations in cardiac metabolism and function using a sheep model of IUGR. Two‐photon imaging revealed that the left ventricle of IUGR fetuses (at 140–141 d GA) had a reduced optical redox ratio, suggesting a reliance on glycolysis for ATP production. Concurrently, the use of PC‐MRI to measure foetal left ventricular cardiac output (LVCO) revealed a positive correlation between LVCO and redox ratio in IUGR, but not control fetuses. These data suggest that altered heart metabolism in IUGR fetuses is indicative of reduced cardiac output, which may contribute to poor cardiac outcomes in adulthood.
Synthesis and characterisation of folic acid based lanthanide ion probes
Publisher: Elsevier BV
Date: 2014
Intracellular distribution and stability of a luminescent rhenium(i ) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging
Publisher: Oxford University Press (OUP)
Date: 2017
DOI: 10.1039/C6MT00243A
Abstract: Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)
Biomolecule detection in porous silicon based microcavitiesviaeuropium luminescence enhancement
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4TB01409J
Abstract: The ability of a porous silicon microcavity (pSiMC) to act as a luminescence enhancing sensor was confirmed using Eu( iii ) complex labelled streptavidin as a model analyte on a biotin-modified pSiMC.
Synthesis, photophysical and cellular characterisation of folate and methotrexate labelled luminescent lanthanide complexes
Publisher: Elsevier BV
Date: 2018
DOI: 10.1016/J.JINORGBIO.2017.10.003
Abstract: In this work we have developed a series of highly emissive europium(III) and terbium(III) complexes tethered to either folic acid (FA) or methotrexate (MTX), with the aim of developing visual probes that enable the imaging of folate receptors in cancer cells. The synthesis, photophysical properties and cellular behaviour are reported for four new lanthanide Ln(III) complexes, where either FA or MTX are tethered to 1,4,7-tris(carbonylmethyl)-10-(4'-quinolineacetic acid, (7'-acetamido)-1',2'-dihydro-2'-oxo)-1,4,7,10-tetraazacyclododecane Ln(III) complex, and Ln(III)=Eu(III) or Tb(III) herein referred to as Eu-FA, Eu-MTX, Tb-FA or Tb-MTX. All four complexes were found to be sensitive to the presence of the folate receptor in a range of cell lines. The MTX conjugates showed different cellular specificity in an oral adenosquamous carcinoma cell line (CAL-27) compared with the analogous FA conjugates. This suggests that it is viable to explore differences in folate receptors using folate vs. anti-folate probes, with labels that have different emissive properties (e.g. Eu-FA vs. Tb-MTX). The MTX complexes were found to be the most cytotoxic, with Eu-MTX showing greater cytotoxicity than free MTX or the isostructural Tb-MTX. This suggested that there could be a synergistic effect on toxicity for the Eu(III) chelate and the MTX components of the complex.
Aminoacid N-substituted 1,4,7-triazacyclononane and 1,4,7,10-tetraazacyclododecane Zn2+, Cd2+ and Cu2+ complexes. A preparative, potentiometric titration and NMR spectroscopic studyElectronic suppleme
Publisher: Royal Society of Chemistry (RSC)
Date: 2004
DOI: 10.1039/B401763C
Abstract: The pK(a)s and Zn2+, Cd2+ and Cu2+ complexation constants (K) for 1,4,7-tris[(2''S)-acetamido-2''-(methyl-3''-phenylpropionate)]-1,4,7-triazacyclononane, 1, 1,4,7-tris[(2''S)-acetamido-2''-(1''-carboxy-3''-phenylpropane)]-1,4,7-triazacyclononane, H(3)2, 1,4,7-tris[(2''S)-acetamido-2''-(methyl-3''-(1H-3-indolyl)propionate)]-1,4,7-triazacyclononane, 3, and 1,4,7,10-tetrakis[(2''S)-acetamido-2''-(methyl-3''-phenylpropionate)]-1,4,7,10-tetraazacyclododecane, 4, 1,4,7,10-tetrakis[(2''S)-acetamido-2''-(1''-carboxy-3''-phenylpropane)]-1,4,7,10-tetraazacyclododecane, H(4)5, in 20 : 80 v/v water-methanol solution are reported. The pK(a)s within the potentiometric detection range for H(3)1(3+) = 8.69 and 3.59, for H(6)2(3+) = 9.06, 6.13, 4.93 and 4.52, H(3)3(3+) = 8.79 and 3.67, H(4)4(4+) = 8.50, 5.62 and 3.77 and for H(8)5(4+) = 9.89, 7.06, 5.53, 5.46, 4.44 and 4.26 where each tertiary amine nitrogen is protonated. The complexes of 1: [Zn(1)]2+(9.00), [Cd(1)]2+ (6.49), [Cd(H1)]3+ (4.54) and [Cu(1)]2+ (10.01) are characterized by the log(K/dm3 mol(-1)) values shown in parentheses. Analogous complexes are formed by 3 and 4: [Zn(3)]2+ (10.19), [Cd(3)]2+ (8.54), [Cu(3)]2+ (10.77), [Zn(4)]2+ (11.41) [Cd(4)]2+ (9.16), [Cd(H4)]3+ (6.16) and [Cu(4)]2+ (11.71). The tricarboxylic acid H(3)2 generates a greater variety of complexes as exemplified by: [Zn(2)-] (10.68) [Zn(H2)] (6.60) [Zn(H(2)2)+] (5.15), [Cd(2)](-) (4.99), [Cd(H2)] (4.64), [Cd(H2(2))]+ (3.99), [Cd(H(3)2)]2+ (3.55), [Cu(2)](-) (12.55) [Cu(H2)] (7.66), [Cu(H(2)2)]+ (5.54) and [Cu(2)2](4-) (3.23). The complexes of H(4)5 were insufficiently soluble to study in this way. The 1H and 13C NMR spectra of the ligands are consistent with formation of a predominant Zn2+ and Cd2+ Delta or Lambda diastereomer. The preparations of the new pendant arm macrocycles H(3)2, 3, 4 and H(4)5 are reported.
pH driven self-assembly of a ternary lanthanide luminescence complex: the sensing of anions using a β-diketonate-Eu(iii ) displacement assay
Publisher: Royal Society of Chemistry (RSC)
Date: 2007
DOI: 10.1039/B611487C
Abstract: The synthesis and the photophysical evaluation of a novel pH dependent lanthanide luminescent self-assembly in water between a cyclen based europium complex and a beta-diketonate is described and its use as a luminescent sensor in displacement assays for anions such as acetate, bicarbonate and lactate, where the Eu(III) emission was quenched upon anion recognition.
In utero substrate restriction by placental insufficiency or maternal undernutrition decreases optical redox ratio in foetal perirenal fat
Publisher: Wiley
Date: 10-01-2021
Tri- and tetra-substituted cyclen based lanthanide(iii ) ion complexes as ribonuclease mimics: a study into the effect of log Ka, hydration and hydrophobicity on phosphodiester hy
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4OB02384F
Abstract: Lanthanide( iii ) complexes of ‘pseudo’ dipeptide ligands and 3'-pyridine ligands have been characterised as metallo-ribonuclease mimics.
Luminescent protein staining with Re(i ) tetrazolato complexes
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8DT02052C
Abstract: Re( i ) tricarbonyl diiimine complexes for the first time exploited as luminescent staining agents for SDS-PAGE.
Investigating Intracellular Localisation and Cytotoxicity Trends for Neutral and Cationic Iridium Tetrazolato Complexes in Live Cells
Publisher: Wiley
Date: 07-08-2017
Abstract: A family of five neutral cyclometalated iridium(III) tetrazolato complexes and their methylated cationic analogues have been synthesised and characterised. The complexes are distinguished by variations of the substituents or degree of π conjugation on either the phenylpyridine or tetrazolato ligands. The photophysical properties of these species have been evaluated in organic and aqueous media, revealing predominantly a solvatochromic emission originating from mixed metal-to-ligand and ligand-to-ligand charge transfer excited states of triplet multiplicity. These emissions are characterised by typically long excited-state lifetimes (∼hundreds of ns), and quantum yields around 5-10 % in aqueous media. Methylation of the complexes caused a systematic red-shift of the emission profiles. The behaviour and the effects of the different complexes were then examined in cells. The neutral species localised mostly in the endoplasmic reticulum and lipid droplets, whereas the majority of the cationic complexes localised in the mitochondria. The amount of complexes found within cells does not depend on lipophilicity, which potentially suggests erse uptake mechanisms. Methylated analogues were found to be more cytotoxic compared to the neutral species, a behaviour that might to be linked to a combination of uptake and intracellular localisation.
Nanoassembled Capsules as Delivery Vehicles for Large Payloads of High Relaxivity Gd3+ Agents
Publisher: American Chemical Society (ACS)
Date: 07-10-2009
DOI: 10.1021/JA906981W
Neutral Re(I) Complex Platform for Live Intracellular Imaging
Publisher: American Chemical Society (ACS)
Date: 07-2021
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