ORCID Profile
0000-0001-8150-1867
Current Organisation
University of South Australia
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Publisher: American Chemical Society (ACS)
Date: 03-01-2022
DOI: 10.1021/ACS.ANALCHEM.1C04470
Abstract: Continued advances in label-free electrical biosensors pave the way to simple, rapid, cost-effective, high-sensitivity, and quantitative biomarker testing at the point-of-care setting that would profoundly transform healthcare. However, implementation in routine diagnostics is faced with significant challenges associated with the inherent requirement for biofluid s le processing before and during testing. We present here a simple yet robust autonomous finger-prick blood s le processing platform integrated with nanoscale field-effect transistor biosensors and demonstrate the feasibility of measuring the SARS-CoV-2 nucleocapsid protein. The 3D-printed platform incorporates a high-yield blood-to-plasma separation module and a delay valve designed to terminate the assay at a specific time. The platform is driven by hydrostatic pressure to efficiently and automatically dispense plasma and washing/measurement buffer to the nanosensors. Our model study demonstrates the feasibility of detecting down to 1.4 pg/mL of the SARS-CoV-2 nucleocapsid protein within 25 min and with only minimal operator intervention.
Publisher: MDPI AG
Date: 15-01-2019
DOI: 10.3390/S19020323
Abstract: Surface plasmon enhanced light scattering (SP-LS) is a powerful new sensing SPR modality that yields excellent sensitivity in sandwich immunoassay using spherical gold nanoparticle (AuNP) tags. Towards further improving the performance of SP-LS, we systematically investigated the AuNP size effect. Simulation results indicated an AuNP size-dependent scattered power, and predicted the optimized AuNPs sizes (i.e., 100 and 130 nm) that afford extremely high signal enhancement in SP-LS. The maximum scattered power from a 130 nm AuNP is about 1700-fold higher than that obtained from a 17 nm AuNP. Experimentally, a bio-conjugation protocol was developed by coating the AuNPs with mixture of low and high molecular weight PEG molecules. Optimal IgG antibody bioconjugation conditions were identified using physicochemical characterization and a model dot-blot assay. Aggregation prevented the use of the larger AuNPs in SP-LS experiments. As predicted by simulation, AuNPs with diameters of 50 and 64 nm yielded significantly higher SP-LS signal enhancement in comparison to the smaller particles. Finally, we demonstrated the feasibility of a two-step SP-LS protocol based on a gold enhancement step, aimed at enlarging 36 nm AuNPs tags. This study provides a blue-print for the further development of SP-LS biosensing and its translation in the bioanalytical field.
Publisher: MDPI AG
Date: 11-05-2018
DOI: 10.3390/MA11050785
Publisher: Elsevier BV
Date: 03-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9RA08605F
Abstract: In the present work, we investigate systematically the electronic and optical properties of Janus ZrSSe using first-principles calculations.
Publisher: Wiley
Date: 06-2019
Publisher: Elsevier BV
Date: 2020
Publisher: MDPI AG
Date: 17-11-2022
DOI: 10.3390/MOLECULES27227952
Abstract: Metal-oxide semiconducting materials are promising for building high-performance field-effect transistor (FET) based biochemical sensors. The existence of well-established top-down scalable manufacturing processes enables the reliable production of cost-effective yet high-performance sensors, two key considerations toward the translation of such devices in real-life applications. Metal-oxide semiconductor FET biochemical sensors are especially well-suited to the development of Point-of-Care testing (PoCT) devices, as illustrated by the rapidly growing body of reports in the field. Yet, metal-oxide semiconductor FET sensors remain confined to date, mainly in academia. Toward accelerating the real-life translation of this exciting technology, we review the current literature and discuss the critical features underpinning the successful development of metal-oxide semiconductor FET-based PoCT devices that meet the stringent performance, manufacturing, and regulatory requirements of PoCT.
Publisher: Elsevier BV
Date: 2020
Publisher: American Chemical Society (ACS)
Date: 04-09-2019
DOI: 10.1021/ACS.ANALCHEM.9B03559
Abstract: Potentiometric sensors based on silicon nanowire field effect transistors (SiNW FETs) typically display exquisite sensitivities, but their bioanalytical implementation is limited due to the need for stringent measurement conditions and high-precision readout units. An alternative operation principle where SiNW FETs are operated in a frequency-domain electrical impedimetric approach is promising. However, to date only limited data is available in regard to the sensing performance and translational relevance of this novel approach in comparison to the standard charge detection paradigm. We demonstrate the feasibility of conducting electrical impedimetric FET measurements with a portable unit for the ultrasensitive detection of cancer biomarkers in biospecimens. Compared to standard potentiometric measurements, electrical impedimetric FET measurements yielded significant improvements in biosensing performances, including the limit of detection, sensing resolution, and dynamic range.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1NR02461B
Abstract: We first present and pilot clinically validate a fully integrated, high-performance indium oxide nano-transistor biodiagnostic platform for rapid, accurate testing of preecl sia without analytical equipment aids and minimum operator intervention.
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9NA00592G
Abstract: High performance indium oxide nanoribbon FETs: mitigating device-to-device signal variation in the subthreshold regime.
Start Date: 2018
End Date: 2021
Funder: Australian Research Council
View Funded ActivityStart Date: 2011
End Date: 2014
Funder: National Health and Medical Research Council
View Funded ActivityStart Date: 2012
End Date: 2013
Funder: Deutscher Akademischer Austauschdienst
View Funded ActivityStart Date: 2018
End Date: 2019
Funder: Deutscher Akademischer Austauschdienst
View Funded ActivityStart Date: 2013
End Date: 2013
Funder: IEEE Foundation
View Funded ActivityStart Date: 2013
End Date: 2013
Funder: University of South Australia
View Funded Activity