ORCID Profile
0000-0002-8320-3668
Current Organisations
University of South Australia
,
South Australian Health and Medical Research Institute
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Publisher: Elsevier BV
Date: 10-2018
DOI: 10.1016/J.PLEFA.2018.08.001
Abstract: Oxylipins are biologically important lipid mediators that are derived enzymatically from polyunsaturated fatty acids (PUFA) and have a major role in regulating inflammatory processes. The currently available methods for measuring oxylipins from human biological s les have limitations, which restricts their use in large studies. We have developed a novel method for measuring 21 oxylipins from dried blood spot (DBS) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and stable isotope dilution analysis. Our new method is reproducible and precise and enables the high throughput analysis and quantitation of bioactive oxylipins in small volumes of blood. In the future, this new method can be readily applied to measure oxylipins in large studies. Abstract Oxylipins are downstream lipid mediators enzymatically-produced from polyunsaturated fatty acids (PUFA) that are implicated as the biological effectors of these fatty acids. Recently reported methods for the quantitation of oxylipins require complex extraction procedures. In this study, we report the development and validation of a novel system for the quantitation of 21 in idual oxylipins from a dried blood spot (DBS) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and stable isotope dilution analysis. Linearity and precision of the method were determined and the stabilities of the 12 most abundant oxylipins were tested during 2 months of storage at room temperature, after being spiked into blood and prepared as DBS on PUFAcoat™ paper. Responses were linear across the concentration range analysed for all oxylipins (r
Publisher: Elsevier BV
Date: 10-2017
DOI: 10.1016/J.PLEFA.2017.08.010
Abstract: Omega-3 and omega-6 polyunsaturated free fatty acids (PUFA-FFA) are precursors to potent downstream lipid mediators that are regulators of inflammation. We describe the development and validation of a novel and sensitive method for quantification of in idual PUFA-FFA in a dried blood spot using liquid chromatography tandem mass spectrometry (LC-MS/MS). Lipids were extracted from dried blood spot and six in idual PUFA-FFA were quantified by LC-MS/MS using stable isotope dilution analysis with deuterated internal standards. PUFA-FFA concentrations in blood s les from 30 subjects were measured using the new method and compared to the traditional approach of thin layer chromatography followed by gas chromatography with flame ionization detection (TLC-GC). Responses for each PUFA-FFA were linear throughout a range of concentrations expected in clinical s les. Intra-day and inter-day variations for all PUFA-FFA were ≤ 16%. The concentrations of all PUFA-FFA measured by LC-MS/MS were positively correlated with measures of the same PUFA-FFA obtained by a traditional TLC-GC method. This novel method for the quantification of PUFA-FFA extracted from dried blood is sensitive and precise, and accurately measures levels of biologically important PUFA-FFA in blood.
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 04-05-2023
DOI: 10.2215/CJN.0000000000000155
Abstract: Transplanted women have high rates of preecl sia. However, determinants of preecl sia and association with graft survival and function remain uncertain. We aimed to determine rates of preecl sia and its association with kidney transplant survival and function. This was a retrospective cohort study analyzing postkidney transplantation pregnancies (≥20 weeks gestation) from the Australia and New Zealand Dialysis and Transplant Registry (2000–2021). Graft survival was assessed in three models accounting for repeated pregnancies and episodes of preecl sia. Preecl sia status was captured in 357 of 390 pregnancies and occurred in 133 pregnancies (37%). The percentage of pregnancies reported to have preecl sia rose from 27% in 2000–2004 to 48% from 2018 to 2021. Reported prior exposure to calcineurin inhibitors was high overall and higher in women who had preecl sia (97% versus 88%, P = 0.005). Seventy-two (27%) graft failures were identified after a pregnancy, with a median follow-up of 8.08 years. Although women with preecl sia had higher median preconception serum creatinine concentration (1.24 [interquartile range, 1.00–1.50] versus 1.13 [0.99–1.36] mg/dl P = 0.02), in all survival models, preecl sia was not associated with higher death-censored graft failure. In multivariable analysis of maternal factors (age, body mass index, primary kidney disease and transplant-pregnancy interval, preconception serum creatinine concentration, era of birth event, and tacrolimus or cyclosporin exposure), only era and preconception serum creatinine concentration ≥1.24 mg/dl (odds ratio, 2.48 95% confidence interval [CI], 1.19 to 5.18) were associated with higher preecl sia risk. Both preconception eGFR ml/min per 1.73 m 2 (adjusted hazard ratio [HR], 5.55 95% CI, 3.27 to 9.44, P 0.001) and preconception serum creatinine concentration ≥1.24 mg/dl (adjusted HR, 3.06 95% CI, 1.77 to 5.27, P 0.001) were associated with a higher risk of graft failure even after adjusting for maternal characteristics. In this large and contemporaneous registry cohort, preecl sia was not associated with worse graft survival or function. Preconception kidney function was the main determinant of graft survival.
Publisher: Elsevier BV
Date: 06-2022
Publisher: Cambridge University Press (CUP)
Date: 14-07-2021
DOI: 10.1017/S0007114520002500
Abstract: Infants born preterm miss out on the peak period of in utero DHA accretion to the brain during the last trimester of pregnancy which is hypothesised to contribute to the increased prevalence of neurodevelopmental deficits in this population. This study aimed to determine whether DHA supplementation in infants born preterm improves attention at 18 months’ corrected age. This is a follow-up of a subset of infants who participated in the N3RO randomised controlled trial. Infants were randomised to receive an enteral emulsion of high-dose DHA (60 mg/kg per d) or no DHA (soya oil – control) from within the first days of birth until 36 weeks’ post-menstrual age. The assessment of attention involved three tasks requiring the child to maintain attention on toy/s in either the presence or absence of competition or a distractor. The primary outcome was the child’s latency of distractibility when attention was focused on a toy. The primary outcome was available for seventy-three of the 120 infants that were eligible to participate. There was no evidence of a difference between groups in the latency of distractibility (adjusted mean difference: 0·08 s, 95 % CI –0·81, 0·97 P = 0·86). Enteral DHA supplementation did not result in improved attention in infants born preterm at 18 months’ corrected age.
Publisher: Wiley
Date: 28-09-2022
DOI: 10.1111/CEN.14604
Abstract: Women with polycystic ovary syndrome (PCOS) report delayed diagnosis of the condition and receiving inadequate information at diagnosis. No studies have investigated the diagnosis experiences of adolescents with PCOS. Our objective was to investigate the adolescents' experiences of PCOS diagnosis and their concerns about the condition. Cross‐sectional study. Eighty‐six adolescents (aged 13–19 years) were diagnosed with PCOS by a medical practitioner. Adolescents were recruited consecutively from paediatric and women's outpatient hospital clinics in South Australia and online PCOS support organisations in Australia and the United Kingdom (May 2017–June 2019). PCOS diagnosis experience and information received at the time of diagnosis were evaluated using a validated questionnaire. The majority of the adolescents ( n = 67, 78%) were diagnosed with PCOS in less than 1 year from their first doctor's visit but 11 (13%) were diagnosed more than 2 years from that visit. Fifty‐three adolescents (66%) saw 1–2 health professionals before the diagnosis was made. Forty‐nine adolescents (57%) were satisfied with the overall diagnosis experience but adolescents were either dissatisfied or reported that the information was not mentioned after diagnosis in relation to lifestyle management ( n = 47, 55%), long‐term complications ( n = 53, 62%) and emotional support and counselling ( n = 65, 76%). The majority of adolescent girls with PCOS are receiving a timely diagnosis, but delayed diagnosis still occurs in a minority of adolescents. Current information provided at diagnosis is not meeting the needs of adolescents and is a lost opportunity for preventive healthcare at a critical transition to adult care period.
Publisher: JMIR Publications Inc.
Date: 05-2019
Abstract: olycystic Ovary Syndrome (PCOS) is a common endocrine condition characterized by irregular periods and hyperandrogenism. Adolescents with PCOS have impaired quality of life (QOL) and increased psychological distress. Transcendental Meditation (TM) is a well-established self-management strategy that has been used to improve stress and well-being. A meta-analysis of TM trials has shown beneficial effects on stress and blood pressure in adults. Recent data are suggesting that another self-management strategy called a mindfulness stress management program has a role in improving QOL in women with PCOS, but there are no studies in adolescents. his study aims to evaluate the effect of TM on QOL and psychological distress in adolescent girls with PCOS. his study is a randomized controlled trial that will be conducted over eight weeks at the Women’s and Children’s Hospital in Adelaide, South Australia, to determine the effect of TM on QOL and psychological distress in adolescent girls (aged 12-20 years) with PCOS. A total of 40 girls will be randomized into either the TM (n=20) or control group (n=20). The TM group will be asked to practice TM in a comfortable sitting position with the eyes closed, for 15 minutes twice daily over eight weeks. The control group will be asked to sit quietly for 15 minutes twice daily for eight weeks. The primary outcomes are any effects on improving QOL and psychological distress, and the secondary outcomes are any effects on lowering blood pressure and salivary cortisol levels. he recruitment of study participants began in May 2019 and is expected to be completed by June 2020. It is expected that the adolescent girls with PCOS practicing TM over eight weeks will have a significant improvement in QOL and psychological distress compared to adolescents in the control group. Also, it is expected that adolescent girls in the TM group will have lower salivary cortisol levels and lower blood pressure. his study will be the first to evaluate the effect of TM on QOL in adolescent girls with PCOS. The study will provide valuable information on a potential self-management strategy to improve QOL and well-being in adolescent girls with PCOS. ustralian New Zealand Clinical Trials Registry (ANZCTR) ACTRN1261900019010 www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=376657& isReview=true RR1-10.2196/14542
Publisher: BMJ
Date: 05-2018
Publisher: Springer Science and Business Media LLC
Date: 04-11-2016
Publisher: Elsevier BV
Date: 07-2022
Publisher: JMIR Publications Inc.
Date: 24-01-2020
DOI: 10.2196/14542
Abstract: Polycystic Ovary Syndrome (PCOS) is a common endocrine condition characterized by irregular periods, hirsutism, acne, or hyperandrogenemia. Adolescents with PCOS have impaired quality of life (QOL) and increased psychological distress. Transcendental Meditation (TM) is a well-established self-management strategy that has been used to improve stress and well-being. A meta-analysis of TM trials has shown beneficial effects on stress and blood pressure in adults. Recent data are suggesting that another self-management strategy called a mindfulness stress management program has a role in improving QOL in women with PCOS, but there are no studies in adolescents. This study aims to evaluate the effect of TM on QOL and psychological distress in adolescent girls with PCOS. This study is a randomized controlled trial that will be conducted over eight weeks at the Women’s and Children’s Hospital in Adelaide, South Australia, to determine the effect of TM on QOL and psychological distress in adolescent girls (aged 12-20 years) with PCOS. A total of 40 girls will be randomized into either the TM (n=20) or control group (n=20). The TM group will be asked to practice TM in a comfortable sitting position with the eyes closed, for 15 minutes twice daily over eight weeks. The control group will be asked to sit quietly for 15 minutes twice daily for eight weeks. The primary outcomes are any effects on improving QOL and psychological distress, and the secondary outcomes are any effects on lowering blood pressure and salivary cortisol levels. The recruitment of study participants began in May 2019 and is expected to be completed by June 2020. It is expected that the adolescent girls with PCOS practicing TM over eight weeks will have a significant improvement in QOL and psychological distress compared to adolescents in the control group. Also, it is expected that adolescent girls in the TM group will have lower salivary cortisol levels and lower blood pressure. This study will be the first to evaluate the effect of TM on QOL in adolescent girls with PCOS. The study will provide valuable information on a potential self-management strategy to improve QOL and well-being in adolescent girls with PCOS. Australian New Zealand Clinical Trials Registry (ANZCTR) ACTRN1261900019010 www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=376657& isReview=true
Publisher: Wiley
Date: 06-12-2021
DOI: 10.1111/CTR.14151
Publisher: Wiley
Date: 23-11-2018
Location: Australia
No related grants have been discovered for Erandi Hewawasam.