ORCID Profile
0000-0002-8250-1639
Current Organisation
University of South Australia
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In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Physical Chemistry of Materials | Functional Materials | Colloid and Surface Chemistry | Polymerisation Mechanisms | Materials Engineering | Physical Chemistry (Incl. Structural) | Materials Engineering not elsewhere classified | Plasma Physics; Fusion Plasmas; Electrical Discharges
Expanding Knowledge in the Chemical Sciences | Health Related to Ageing | Expanding Knowledge in the Physical Sciences | Expanding Knowledge in Engineering | Expanding Knowledge in the Biological Sciences | Manufacturing not elsewhere classified |
Publisher: Royal Society of Chemistry (RSC)
Date: 2003
DOI: 10.1039/B305445B
Publisher: American Chemical Society (ACS)
Date: 29-09-2017
Publisher: Elsevier BV
Date: 03-2022
Publisher: American Chemical Society (ACS)
Date: 08-03-2000
DOI: 10.1021/CM9907413
Publisher: Wiley
Date: 08-06-2011
Publisher: American Chemical Society (ACS)
Date: 12-08-2000
DOI: 10.1021/CM0002158
Publisher: Oxford University Press (OUP)
Date: 03-09-2009
Publisher: Elsevier BV
Date: 02-2013
Publisher: Oxford University Press (OUP)
Date: 09-2013
DOI: 10.1093/JRS/FET021
Publisher: Elsevier BV
Date: 04-2021
Publisher: American Chemical Society (ACS)
Date: 22-07-2020
Publisher: Wiley
Date: 12-02-2015
Publisher: MDPI AG
Date: 31-03-2019
DOI: 10.3390/ANTIBIOTICS8020034
Abstract: Plant metabolites that have shown activity against bacteria and/or environmental fungi represent valuable leads for the identification and development of novel drugs against clinically important human pathogenic fungi. Plants from the genus Eremophila were highly valued in traditional Australian Aboriginal medicinal practices, and E. alternifolia was the most prized among them. As antibacterial activity of extracts from E. alternifolia has been documented, this study addresses the question whether there is also activity against infectious fungal human pathogens. Compounds from leaf-extracts were purified and identified by 1- and 2-D NMR. These were then tested by disk diffusion and broth microdilution assays against ten clinically and environmentally relevant yeast and mould species. The most potent activity was observed with the diterpene compound, 8,19-dihydroxyserrulat-14-ene against Cryptococcus gattii and Cryptococcus neoformans, with minimum inhibition concentrations (MIC) comparable to those of Amphotericin B. This compound also exhibited activity against six Candida species. Combined with previous studies showing an antibacterial effect, this finding could explain a broad antimicrobial effect from Eremophila extracts in their traditional medicinal usage. The discovery of potent antifungal compounds from Eremophila extracts is a promising development in the search for desperately needed antifungal compounds particularly for Cryptococcus infections.
Publisher: Elsevier BV
Date: 09-2021
Publisher: American Chemical Society (ACS)
Date: 22-11-2016
Abstract: Skin has a remarkable capacity for regeneration however, with an ever aging population, there is a growing burden to the healthcare system from chronic wounds. Novel therapies are required to address the problems associated with nonhealing chronic wounds. Novel wound dressings that can encourage increased reepithelialization could help to reduce the burden of chronic wounds. A suite of chemically defined surfaces have been produced using plasma polymerization, and the ability of these surfaces to support the growth of primary human skin cells has been assessed. Additionally, the ability of these surfaces to modulate cell migration and morphology has also been investigated. Keratinocytes and endothelial cells were extremely sensitive to surface chemistry showing increased viability and migration with an increased number of carboxylic acid functional groups. Fibroblasts proved to be more tolerant to changes in surface chemistry however, these cells migrated fastest over amine-functionalized surfaces. The novel combination of comprehensive chemical characterization coupled with the focus on cell migration provides a unique insight into how a material's physicochemical properties affect cell migration.
Publisher: Royal Society of Chemistry (RSC)
Date: 2003
DOI: 10.1039/B302176A
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4BM00018H
Abstract: The cooperative effects of FGF-2, surface bound heparin and plasma polymer, produce a biologically active surface which increases the half-life of FGF-2. The production of this surface is simple and requires no chemical cross-linking.
Publisher: American Vacuum Society
Date: 11-2000
DOI: 10.1116/1.1316099
Publisher: Wiley
Date: 07-01-2014
Publisher: Springer Science and Business Media LLC
Date: 04-02-2014
DOI: 10.1007/S10856-014-5163-Y
Abstract: Age related macular degeneration of the eye is brought about by damage to the retinal pigment epithelium (RPE) and is a major cause of adult blindness. One potential treatment method is transplantation of RPE cells grown in vitro. Maintaining RPE cell viability and physiological function in vitro is a challenge, and this must also be achieved using materials that can be subsequently used to deliver an intact cell sheet into the eye. In this paper, plasma polymerisation has been used to develop a chemically modified surface for maintaining RPE cells in vitro. Multiwell plates modified with a plasma copolymer of allylamine and octadiene maintained RPE cell growth at a level similar to that of TCPS. However, the addition of bound glycosaminoglycans (GAGs) to the plasma polymerised surface significantly enhanced RPE proliferation. Simply adding GAG to the culture media had no positive effect. It is shown that a combination of plasma polymer and GAG is a promising method for developing suitable surfaces for cell growth and delivery, that can be applied to any substrate material.
Publisher: Frontiers Media SA
Date: 04-02-2015
Publisher: MDPI AG
Date: 06-08-2021
DOI: 10.3390/MOLECULES26164762
Abstract: If plasma polymer thin films are to be synthesised from sustainable and natural precursors of chemically heterogeneous composition, it is important to understand the extent to which this composition influences the mechanism of polymerisation. To this end, a well-studied monoterpene alcohol, terpinen-4-ol, has been targeted for a comparative study with the naturally occurring mix of terpenes (viz. Melaleuca alternifolia oil) from which it is commonly distilled. Positive ion mode mass spectra of both terpinen-4-ol and M. alternifolia oil showed a decrease in disparities between the type and abundance of cationic species formed in their respective plasma environments as applied plasma power was increased. Supplementary biological assay revealed the antibacterial action of both terpinen-4-ol and M. alternifolia derived coatings with respect to S. aureus bacteria, whilst cytocompatibility was demonstrated by comparable eukaryotic cell adhesion to both coatings. Elucidating the processes occurring within the reactive plasmas can enhance the economics of plasma polymer deposition by permitting use of the minimum power, time and precursor pre-processing required to control the extent of monomer fragmentation and fabricate a film of the desired thickness and functionality.
Publisher: American Chemical Society (ACS)
Date: 22-07-2004
DOI: 10.1021/JP048250F
Publisher: Elsevier BV
Date: 02-2018
DOI: 10.1016/J.JCYT.2017.11.004
Abstract: This review aims to provide a broad introduction to the use of cell sheets and the role of materials in the delivery of cell sheets to patients within a clinical setting. Traditionally, cells sheets have been, and currently are, fabricated using established and accepted cell culture methods within standard formats (e.g., petri dishes) utilizing biological substrates. Synthetic surfaces provide a far more versatile system for culturing and delivering cell sheets. This has the potential to positively affect quality, and efficient, localized cell delivery has a significant impact on patient outcome and on the overall cost of goods. We highlight current applications of these advanced carriers and future applications of these surfaces and cell sheets with an emphasis both on clinical use and regulatory requirements.
Publisher: MDPI AG
Date: 02-01-2023
DOI: 10.3390/IJMS24010797
Abstract: Fundamental knowledge about cell–surface interactions can be applied in the development of wound dressings and scaffolds to encourage wounds to heal. As surfaces produced with acid-functionalised monomers encourage keratinocyte adhesion, proliferation and migration, whilst amine functionalisation enhances fibroblast proliferation and migration in vitro, standard care wound dressings were plasma-coated with either acrylic acid or allylamine and applied to 6 mm excisional wounds on the backs of mice to test their effectiveness in vivo. At day 3, the rate of wound healing was increased in mice treated with dressings that were plasma-coated with allylamine compared to uncoated dressings, with a significantly reduced wound area. However, healing may be impaired following prolonged treatment with allylamine-functionalised dressings, with delayed re-epithelialisation and increased cellularisation of the wound site at later timepoints. Acrylic acid functionalisation, however, offered no early improvement in wound healing, but wounds treated with these dressings displayed increased collagen deposition at day 7 post wounding. These results suggest that plasma polymerisation may allow for the development of new dressings which can enhance wound closure by directing cell behaviour, but that the application of these dressings may require a timed approach to enhance specific phases of the wound healing response.
Publisher: BMJ
Date: 12-2021
DOI: 10.1136/BMJOPEN-2021-052156
Abstract: To investigate the additional programme cost and cost-effectiveness of ‘right@home’ Nurse Home Visiting (NHV) programme in relation to improving maternal and child outcomes at child age 3 years compared with usual care. A cost–utility analysis from a government-as-payer perspective alongside a randomised trial of NHV over 3-year period. Costs and quality-adjusted life-years (QALYs) were discounted at 5%. Analysis used an intention-to-treat approach with multiple imputation. The right@home was implemented from 2013 in Victoria and Tasmania states of Australia, as a primary care service for pregnant women, delivered until child age 2 years. 722 pregnant Australian women experiencing adversity received NHV (n=363) or usual care (clinic visits) (n=359). First, a cost–consequences analysis to compare the additional costs of NHV over usual care, accounting for any reduced costs of service use, and impacts on all maternal and child outcomes assessed at 3 years. Second, cost–utility analysis from a government-as-payer perspective compared additional costs to maternal QALYs to express cost-effectiveness in terms of additional cost per additional QALY gained. When compared with usual care at child age 3 years, the right@home intervention cost $A7685 extra per woman (95% CI $A7006 to $A8364) and generated 0.01 more QALYs (95% CI −0.01 to 0.02). The probability of right@home being cost-effective by child age 3 years is less than 20%, at a willingness-to-pay threshold of $A50 000 per QALY. Benefits of NHV to parenting at 2 years and maternal health and well-being at 3 years translate into marginal maternal QALY gains. Like previous cost-effectiveness results for NHV programmes, right@home is not cost-effective at 3 years. Given the relatively high up-front costs of NHV, long-term follow-up is needed to assess the accrual of health and economic benefits over time. ISRCTN89962120 .
Publisher: Elsevier BV
Date: 02-2012
DOI: 10.1016/J.BIOMATERIALS.2011.10.042
Abstract: Glycosaminoglycans play an important role in tissue organisation through interactions with a erse range of proteins, growth factors and other chemokines. In this report, we demonstrate the GAG-binding 'fingerprint' of two important GAG-binding proteins - osteoprotogerin and TIMP-3. The technique uses a straightforward method for attaching GAGs to assay surfaces in a non-covalent manner using plasma polymerization that leaves the adsorbed GAG able to participate in subsequent ligand binding. We show that OPG and TIMP-3 bind preferentially to different GAGs in a simple ELISA and that this binding does not correlate directly with simple GAG properties such as degree of sulfation. The methods outlined in this report can be easily applied to tissue engineering scaffolds in order to exploit the potential of surface-bound GAGs in influencing the structure of engineered tissues.
Publisher: Elsevier BV
Date: 2021
Publisher: Wiley
Date: 04-06-2014
Publisher: American Chemical Society (ACS)
Date: 14-06-2013
DOI: 10.1021/AM401484B
Abstract: External parameters (RF power and precursor flow rate) are typically quoted to define plasma polymerization experiments. Utilizing a parallel-plate electrode reactor with variable geometry, it is shown that these parameters cannot be transferred to reactors with different geometries in order to reproduce plasma polymer films using four precursors. Measurements of ion flux and power coupling efficiency confirm that intrinsic plasma properties vary greatly with reactor geometry at constant applied RF power. It is further demonstrated that controlling intrinsic parameters, in this case the ion flux, offers a more widely applicable method of defining plasma polymerization processes, particularly for saturated and allylic precursors.
Publisher: Wiley
Date: 18-10-2006
DOI: 10.1002/SIA.2400
Publisher: American Vacuum Society
Date: 09-2016
DOI: 10.1116/1.4962267
Abstract: Furfuryl methacrylate (FMA) is a promising precursor for producing polymers for biomedical and cell therapy applications. Herein, FMA plasma polymer coatings were prepared with different powers, deposition times, and flow rates. The plasma polymer coatings were characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The results from AFM and SEM show the early growth of the coatings and the existence of particle aggregates on the surfaces. XPS results indicated no measureable chemical differences between the deposited films produced under different power and flow rate conditions. ToF-SIMS analysis demonstrated differing amounts of C5H5O (81 m/z) and C10H9O2 (161 m/z) species in the coatings which are related to the furan ring structure. Through judicious choice of plasma polymerization parameters, the quantity of the particle aggregates was reduced, and the fabricated plasma polymer coatings were chemically uniform and smooth. Primary human fibroblasts were cultured on FMA plasma polymer surfaces to determine the effect of surface chemical composition and the presence of particle aggregates on cell culture. Particle aggregates were shown to inhibit fibroblast attachment and proliferation.
Publisher: American Chemical Society (ACS)
Date: 25-09-2019
Publisher: Springer Science and Business Media LLC
Date: 10-11-2021
Publisher: Springer Science and Business Media LLC
Date: 12-2016
Publisher: American Chemical Society (ACS)
Date: 20-03-2009
DOI: 10.1021/LA803775M
Abstract: We describe a method to produce antibody-captured ligand gradients over biologically relevant distances (hundreds of micrometers) whereby the ligand density and gradient shape may be tailored. Separation of the ligand from the solid-phase surface ensures that the biological activity of the ligand remains unaffected by immobilization. Our method involves the use of a plasma-masking method to generate a surface chemical gradient on a glass substrate to which the 9E10 antibody is covalently coupled. This antibody captures myc-tagged biomolecules. In our ex le, the antibody is then used to immobilize a gradient of the intercellular signaling molecule delta-like-1 (Dll1). To visualize the gradient of Dll1, we have used the multistep approach of binding with rabbit anti-Dll1 primary antibody and then adding colloidal-gold-conjugated secondary antibody.
Publisher: American Chemical Society (ACS)
Date: 13-02-2013
DOI: 10.1021/LA304713B
Abstract: It has been shown that both ions and neutral species may contribute to plasma polymer growth. However, the relative contribution from these mechanisms remains unclear. We present data elucidating the importance of considering monomer structure with respect to which the growth mechanism dominates for nonfouling PEG-like plasma polymers. The deposition rate for saturated monomers is directly linked with ion flux to the substrate. For unsaturated monomers, the neutral flux also plays a role, particularly at low power. Increased fragmentation of the monomer at high power reduces the ability of unsaturated monomers to grow via neutral grafting. Chemical characterization by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) confirm the role that plasma phase fragmentation plays in determining the deposition rate and surface chemistry of the deposited film. The simple experimental method used here may also be used to determine which mechanisms dominate plasma deposition for other monomers. This knowledge may enable significant improvement in future reactor design and process control.
Publisher: Wiley
Date: 17-06-2013
Publisher: Royal Society of Chemistry (RSC)
Date: 11-07-2002
DOI: 10.1039/B201471H
Publisher: Wiley
Date: 03-04-2015
Publisher: AIP Publishing
Date: 03-02-2022
DOI: 10.1063/5.0061714
Abstract: Layered vanadate cathodes hold promise for aqueous zinc-ion batteries (AZIBs) owing to their multiple redox reactions as well as large interlayer space for Zn2+ storage. However, they are limited by vanadium dissolution during cycling, in association with severe capacity fade and unsatisfactory cyclic life. To address this challenge, we herein report a pre-inserted dual-cation vanadate (NaxZnyV3O8·nH2O) cathode, which combines the Zn2+-reinforced cathode structure with the Na+-enlarged lattice distance for fast and stable Zn2+ migration. Multiple ex situ analysis found that electrochemically active Zn3(OH)2V2O7·2H2O was generated after discharging, and this corresponds to the efficient suppression of vanadium dissolution by strong ionic bonding. As a result, a certain NaxZnyV3O8·nH2O cathode having a Na+ to Zn2+ ratio of 2:1 retains 99.6% of capacity after 418 cycles at 0.1 A g−1, 90.5% after 6000 cycles at 1.0 A g−1, and 96.7% after 9499 cycles at 10.0 A g−1. Our method paves a way for researchers to develop robust cathode materials for ultra-stable AZIBs.
Publisher: Wiley
Date: 20-09-2017
DOI: 10.1002/JBM.B.33999
Abstract: Functionalizing medical devices with polypeptides to enhance their performance has become important for improved clinical success. The extracellular matrix (ECM) adhesion protein vitronectin (VN) is an effective coating, although the chemistry used to attach VN often reduces its bioactivity. In vivo, VN binds the ECM in a sequence-dependent manner with heparan sulfate (HS) glycosaminoglycans. We reasoned therefore that sequence-based affinity chromatography could be used to isolate a VN-binding HS fraction (HS9) for use as a coating material to capture VN onto implant surfaces. Binding avidity and specificity of HS9 were confirmed by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR)-based assays. Plasma polymerization of allylamine (AA) to tissue culture-treated polystyrene (TCPS) was then used to capture and present HS9 as determined by radiolabeling and ELISA. HS9-coated TCPS avidly bound VN, and this layered surface supported the robust attachment, expansion, and maintenance of human pluripotent stem cells. Compositional analysis demonstrated that 6-O- and N-sulfation, as well as lengths greater than three disaccharide units (dp6) are critical for VN binding to HS-coated surfaces. Importantly, HS9 coating reduced the threshold concentration of VN required to create an optimally bioactive surface for pluripotent stem cells. We conclude that affinity-purified heparan sugars are able to coat materials to efficiently bind adhesive factors for biomedical applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1887-1896, 2018.
Publisher: Wiley
Date: 25-01-2016
Publisher: Elsevier BV
Date: 2018
DOI: 10.1016/J.COLSURFB.2017.10.065
Abstract: Human Mesenchymal Stem cells (hMSCs) are becoming a major focus in biomedical fields. Application of in vitro expanded hMSCs to treat numerous ailments has led to a commercial emphasis on improving hMSC growth ex vivo. Production of substrate independent, novel thin films is one potential tool for production of commercial viable hMSC expansion. Plasma polymerization allow controlled chemical optimisation of large scale surface areas in a substrate independent manner. Previous study shown that plasma polymerized Furfuryl Methacrylate (ppFMA) surfaces allowed primary fibroblast cells adhesion and proliferation. However, under some deposition conditions, particle aggregates formation was observed. These aggregates had the effect of disrupting cell attachment, despite being chemically indistinguishable from the underlying surface. Herein, hMSCs were cultured on ppFMA surfaces to determine their suitability for stem cell culture and observe the effect of particle aggregates on hMSC attachment and growth. Both metabolic and DNA quantification assays showed that surfaces with particle aggregates had lower numbers of attached cells and slower growth. Uniform surfaces without aggregates showed higher cell attachment and growth levels, which were comparable to Thermanox. Phenotypic analysis showed that there was no change to hMSCs phenotype after 7 & 14days of culture on uniform ppFMA surface. Further investigation using time-lapse image analysis indicated that particle aggregates reduced cell attachment by presenting a physically weak boundary layer, which was damaged by intracellular tension during cell spreading. ppFMA surface can provide a stable substrate independent hMSCs expansion interface that could be applied to larger scale bioreactors, beads or scaffolds.
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3RA41563E
Publisher: Informa UK Limited
Date: 23-09-2015
Publisher: Elsevier BV
Date: 12-2023
Publisher: Wiley
Date: 18-03-2008
Publisher: Wiley
Date: 27-05-2015
Abstract: Active dressings that based on fabric materials are an area of interest for the treatment of wounds. Poly(l-lactide) nanoparticles containing the antimicrobial agent octenidine can be controllably lysed by toxins released by pathogenic bacteria thus releasing antimicrobial material in response to the presence of the bacterial toxins and so counteracting the infection. We developed an integrated engineering solution that allows for the stable immobilisation of nanoparticles on non-woven fabrics. The process involves coating nanoparticles on non-woven polymer surfaces by using an inkjet printing process. In order to improve the adhesion and retention of the nanoparticles on the fabric, surface pretreatment of the non-woven fabric using plasma jet treatment can be applied to increase its surface energy.
Publisher: Wiley
Date: 10-2019
Publisher: Wiley
Date: 08-2019
Publisher: Wiley
Date: 15-02-2010
Publisher: Informa UK Limited
Date: 09-2001
Publisher: Royal Society of Chemistry (RSC)
Date: 2004
DOI: 10.1039/B311256J
Publisher: Wiley
Date: 18-06-2012
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3SM51039E
Start Date: 09-2011
End Date: 12-2015
Amount: $290,376.00
Funder: Australian Research Council
View Funded ActivityStart Date: 01-2012
End Date: 12-2013
Amount: $150,000.00
Funder: Australian Research Council
View Funded Activity