ORCID Profile
0000-0002-7337-093X
Current Organisations
Krida Wacana Christian University
,
University of South Australia
,
Universitas Indonesia
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Publications
Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography
Publisher: Garval Editorial Ltda.
Date: 11-2023
In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L.
Publisher: Secretariat of The Indonesian Biomedical Journal
Date: 02-08-2018
Abstract: BACKGROUND: The leaf and fruit of Psidium guajava L. are potential for neutraceutical beverage especially for antidiabetic drink. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf (LE) and fruit (FE) from P. guajava.METHODS: Both extracts were investigated for their inhibitory effect on α-glucosidase activity in vitro. Their antioxidant activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferrous ion chelating, reducing power and phosphomolybdate methods.RESULTS: The IC50 of LE, FEandacarbose as a positive control were 5.67, 428.00 and 823.99 μg/mL, respectively. The enzyme kinetic analysis indicated that LE inhibited α-glucosidase in a competitive inhibition type, similar to that of acarbose. Both extracts showed antioxidant activities, with LE showed stronger activities than FE in all methods. In DPPH method, IC50 of LE and FE were 74.77 and 843.84 μg/mL respectively, compared to 53.24 and 21.36 μg/mL for reference antioxidants butylated hydroxytoluene (BHT) and ascorbic acid (AA), respectively. In ferrous ion chelating activity, the IC50 were 147.07 and 2105.05 μg/mL for LE and FE, whereas ethylenediaminetetraacetic acid (EDTA) as a control s le was 66.50 μg/mL. In reducing power and phosphomolybdate methods, at different concentrations, the activities of LE, FE, and standard compounds showed the following order: AA BHT LE FE.CONCLUSION: LE from P. guajava exhibited excellent inhibitory activity against α-glucosidase. In addition, LE had better antioxidant acivities than FE. This study can recommend the aqueous extract from P. guajava as a promising candidate for neutraceutical drink for prediabetic and diabetic patients.KEYWORDS: antioxidant, aqueous extract, α-glucosidase inhibition, guava, Psidium guajava L.
In-vitro antidiabetic effect and antioxidant activity of Premna serratifolia L
Publisher: EManuscript Technologies
Date: 06-06-2017
DOI: 10.5530/PJ.2017.4
In Vitro Antidiabetic and Antioxidant Activities of Aqueous Extract from the Leaf and Fruit of Psidium guajava L.
Publisher: Secretariat of The Indonesian Biomedical Journal
Date: 02-08-2018
Abstract: BACKGROUND: The leaf and fruit of Psidium guajava L. are potential for neutraceutical beverage especially for antidiabetic drink. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf (LE) and fruit (FE) from P. guajava.METHODS: Both extracts were investigated for their inhibitory effect on α-glucosidase activity in vitro. Their antioxidant activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferrous ion chelating, reducing power and phosphomolybdate methods.RESULTS: The IC50 of LE, FEandacarbose as a positive control were 5.67, 428.00 and 823.99 μg/mL, respectively. The enzyme kinetic analysis indicated that LE inhibited α-glucosidase in a competitive inhibition type, similar to that of acarbose. Both extracts showed antioxidant activities, with LE showed stronger activities than FE in all methods. In DPPH method, IC50 of LE and FE were 74.77 and 843.84 μg/mL respectively, compared to 53.24 and 21.36 μg/mL for reference antioxidants butylated hydroxytoluene (BHT) and ascorbic acid (AA), respectively. In ferrous ion chelating activity, the IC50 were 147.07 and 2105.05 μg/mL for LE and FE, whereas ethylenediaminetetraacetic acid (EDTA) as a control s le was 66.50 μg/mL. In reducing power and phosphomolybdate methods, at different concentrations, the activities of LE, FE, and standard compounds showed the following order: AA BHT LE FE.CONCLUSION: LE from P. guajava exhibited excellent inhibitory activity against α-glucosidase. In addition, LE had better antioxidant acivities than FE. This study can recommend the aqueous extract from P. guajava as a promising candidate for neutraceutical drink for prediabetic and diabetic patients.KEYWORDS: antioxidant, aqueous extract, α-glucosidase inhibition, guava, Psidium guajava L.
Chemical Characteristics and In vitro Antidiabetic and Antioxidant Activities of Premna serratifolia L. Leaf Infusion and Decoction
Publisher: EManuscript Technologies
Date: 14-09-2018
α-Glucosidase Inhibitory Effect of Fermented Fruit Juice of Morinda Citrifolia L and Combination Effect with Acarbose
Publisher: Enviro Research Publishers
Date: 04-2019
Abstract: Fermented fruit juice of M.citrifoliais supposed to be the future nutraceutical beverage due to its antidiabetic and antioxidant activities. The purposes of this study were to characterize the fermented juice microbiologically and chemically and to evaluate itsα-glucosidase inhibition and radical scavenging activities in vitro. The fruit of M.citrifolia was fermented and the fruit juice was obtained and evaluated for its radical scavenging activity based on a DPPH assay. It's in vitroanti diabetic activity on α-glucosidase inhibition was investigated, including its combined effect with acarbose by a Chou-Talalay method. The inhibition mode was evaluated by Line weaver-Burk plots. The juice was identified for its microbiome with 16S sequencing method and pictured with SEM. The bioactive compounds were analysed with LC-MS. The main microbiome was yeast and tentatively identified as Candida. The yeast was not able to grow in the normal growth medium for yeast, such as sabouroud agar. The TPC of the juice was 1,193 μg GAE/ml. The main compounds identified by an LC-MS were short-chain fatty acids (α-ketoglutaric acid and malic acid). The fermented fruit juice showed good α-glucosidase inhibitory and antioxidant activities with IC50 of 28.99 and 14.09μgGAE/ml, respectively. The kinetic study showed a non-competitive inhibition on α-glucosidase. The combination of the juice with acarbose at higher concentrations produced an additive effect on α-glucosidase. However, at lower concentrations, an antagonistic effect was observed. The fermented fruit juice of M.citrifoliais a good beverage with strong antidiabetic and antioxidant effects.
Enzyme inhibitory, antioxidant, and antibacterial activities of ethanol fruit extract of Muntingia calabura Linn
Publisher: Maad Rayan Publishing Company
Date: 07-2020
DOI: 10.34172/JHP.2020.44
Abstract: Introduction: Muntingia calabura is used for many medicinal advantages. So far, limited study has been done for the bioactivities of M.calabura fruit. The study aimed to investigate the enzyme inhibitory, antioxidant, and antibacterial activities of M.calabura fruit. Methods: Ethanol extract of M.calabura fruit was tested for its inhibitory enzyme activities against key enzymes linked to human pathologies, such as diabetes (α-glucosidase and α-amylase), hyperuricemia (xanthine oxidase), and obesity (lipase). The antioxidant properties were investigated using different in vitro assays (DPPH, CUPRAC, reducing power, phosphomolybdenum, metal chelating and DNA-Damage protection assays). The fruit was also evaluated for its antibacterial activity against several gram positive and negative bacteria. Results: The total phenolic and flavonoid contents of the extract were 10.85 mgGAE/g and 3.30 mg QE/g, respectively. The fruit extract showed good inhibition against α-glucosidase and α-amylase (IC 50 16.74 and 46.49 µg/ml, respectively), with activities stronger than acarbose (100.38 and 152.46 µg/ml, respectively). It exhibited weak inhibitory activity against xanthine oxidase (IC 50 0.91 mg/ml) and lipase (IC 50 16.48 mg/ml), weaker than the references used for respective test (IC 50 allopurinol 5.31 µg/ml and orlistat 0.17 µg/ml). The extract showed antibacterial activities againts Chromobacterium violaceum, Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Escherichia coli . The ethanol extract showed weaker antioxidant activities, when compared to ascorbic acid and BHT. However, the extract was able to protect DNA-damage. Conclusions: The study concludes that M. calabura fruit exhibits antioxidant, antibacterial, and enzyme inhibitory properties, thus can be a good source for pharmacological uses.
Antidiabetic, Antibacterial and Antioxidant Activities of Different Extracts from Brucea javanica (L.) Merr Seeds
Publisher: EManuscript Technologies
Date: 10-05-2019
Antioxidant, Antidiabetic, and Anti-obesity Potential of Ipomoea reptans Poir Leaves
Publisher: Universitas Muhammadiyah Palangkaraya
Date: 30-11-2020
Abstract: Ipomoea reptans Poir or kangkung is a popular leafy vegetable, a favorite to people in Asian countries. However, limited information is available on their bioactivities. In the present study, the antioxidant, antidiabetic, and anti-obesity potential of I. reptans leaves were investigated. Different fractions (ethanol, ethyl acetate, and hexane) of I. reptans leaves were evaluated for their scavenging activity on DPPH radicals, whereas their reducing potential was investigated by cupric reducing antioxidant capacity (CuPRAC), total antioxidant, and reducing power assays. The antidiabetic potential was investigated by their inhibition effect on a-glucosidase. Total phenolic and flavonoid contents of I. reptans leaves were solvent dependent. Ethyl acetate contained the highest phenolic content, followed by ethanol and hexane fractions. However, for flavonoid content, the order was ethanol ethyl acetate hexane. All fractions showed DPPH scavenging activity in a concentration-dependent manner, with activities weaker than standards ascorbic acid and BHT, in the order of ethanol ethyl acetate hexane. All fractions showed reducing capacity, but only hexane and ethanol fractions of I. reptans leaves showed inhibition on a-glucosidase, with hexane showed more potent inhibition compared to acarbose. The study also found that fractions of I. reptans inhibit lipase and trypsin, enzymes related to lipid metabolism. Findings in this study offer a prospect for I. reptans leaves as a functional food source for antioxidant, antidiabetic, and anti-obesity purposes.
Nutritional Enzymes Inhibitory Activities of Fruit Juice and Fruit Pulp Extract of Muntingia Calabura L.
Publisher: Enviro Research Publishers
Date: 28-12-2020
Abstract: The fruit of Kersen or cherry (Muntingia calabura), a popular shade tree, is not yet promoted as a marketable fruit or a healthy herbal drink. This study aimed to investigate potential health benefits of fruit juice and crude extract of the fruit pulp of M. calabura including their antioxidant potential and inhibitory activities on several enzymes related to metabolic disorders. Fresh juice (FJ) of M.calabura fruit was obtained by juice extractor, whereas the fruit pulp (FP) was dried and macerated with ethanol to obtain the crude extract. Both were evaluated for their inhibitory activities against α-glucosidase, α-amylase, xanthine oxidase, lipase, and protease. Antioxidant activity was evaluated based on their scavenging activity on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radicals. Both FJ and FP showed good antidiabetic activity by inhibiting α-glucosidase (IC50 162.00 and 84.70 µg/mL, respectively) and α-amylase (IC50 422.34 and 80.46 µg/mL, respectively). In comparison, IC50 of acarbose on α-glucosidase and α-amylase were 130.66 and 152.46 µg/mL, respectively. The FJ and FP extract showed anti-hyperuricemia activity by inhibiting xanthine oxidase (0.02 and 2.78 mg allopurinol equivalent/mL for FJ and FP, respectively). However, weak inhibition activities on protease and lipase were observed for the FJ and FP extract compared to the references used. Similarly, FJ and FP exhibited weak scavenging activity on DPPH, when compared with BHT and ascorbic acid. The study recommends the fruit of M.calabura as a potential source of antidiabetic and anti-hyperuricemia agents. The fruit can be further explored for nutraceutical applications.
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