ORCID Profile
0000-0001-5557-6689
Current Organisations
University of South Australia
,
Monash University
,
Bilkent Üniversitesi
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In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Nanomaterials | Nanotechnology | Nanobiotechnology | Nanofabrication, Growth and Self Assembly
Expanding Knowledge in Engineering | Expanding Knowledge in Technology |
Publisher: Cold Spring Harbor Laboratory
Date: 22-07-2022
DOI: 10.1101/2022.07.22.501113
Abstract: With the rapid development of microfluidic based cell therapeutics systems, the need arises for compact, modular, and microfluidics-compatible intracellular delivery platforms with small footprints and minimal operational requirements. Physical deformation of cells passing through a constriction in a microfluidic channel has been shown to create transient membrane perturbations that allow passive diffusion of materials from the outside to the interior of the cell. This mechanical approach to intracellular delivery is simple to implement and fits the criteria outlined above. However, available microfluidic platforms that operate through this mechanism are traditionally constructed from rigid channels with fixed dimensions that suffer from irreversible clogging and incompatibility with larger size distributions of cells. Here we report a flexible and elastically deformable microfluidic channel, and we leverage this elasticity to dynamically generate temporary constrictions with any given size within the channel width parameters. Additionally, clogging is prevented by increasing the size of the constriction to allow clogs to pass. By tuning the size of the constriction appropriately, we show the successful delivery of GFP-coding plasmids to the interior of three mammalian cell lines and fluorescent gold nanoparticles to HEK293 FT cells all while maintaining a high cell viability rate. This development will no doubt lead to miniaturized intracellular delivery microfluidic components that can be easily integrated into larger lab-on-a-chip systems in future cell modification devices.
Publisher: Wiley
Date: 12-2017
Publisher: Wiley
Date: 15-10-2023
Abstract: Permittivity of microscopic particles can be used as a classification parameter for applications in materials and environmental sciences. However, directly measuring the permittivity of in idual microparticles has proven to be challenging due to the convoluting effect of particle size on capacitive signals. To overcome this challenge, we built a sensing platform to independently obtain both the geometric and electric size of a particle, by combining impedance cytometry and microwave resonant sensing in a microfluidic chip. This way the microwave signal, which contains both permittivity and size effects, can be normalized by the size information provided by impedance cytometry to yield an intensive parameter that depends only on permittivity. The technique allowed us to differentiate between polystyrene and soda lime glass microparticles —below 22 microns in diameter— with more than 94% accuracy, despite their similar sizes and electrical characteristics. Furthermore, we show that the same technique can be used to differentiate between normal healthy cells and fixed cells of the same geometric size. The technique offers a potential route for targeted applications such as environmental monitoring of microplastic pollution or quality control in pharmaceutical industry. This article is protected by copyright. All rights reserved
Publisher: Wiley
Date: 24-07-2019
Abstract: Although the negative consequences of the global phenomenon of jellyfish (JF) swarms are well recognized, the use of their biomass for practical applications is mostly limited to a niche in the Asian food industry. This fact is quite surprising since JF's biomass comprises useful biomaterials such as Q‐mucin glycoprotein and collagen. In this work, the JF biomass, collected from two different species, is used to prepare electrospun scaffolds composed of nanometric “core–shell”‐type fibers, in which adjustment of the electrospinning process parameters can easily control their mechanical, morphological, and chemical properties. This nonwoven scaffold shows excellent biocompatibility and biodegradability, indicating suitability for biomedical research contexts. Performed cell proliferation assays show that the scaffold could support the growth of cardiac cells, fitting the requirement of tissue engineering. Additional incorporation of in situ‐generated silver nanoparticles in these nanofibers produced mats with potent antibacterial properties. Preclinical trials with the resulted mats on porcine wound healing models exhibit fast and complete healing of wounds.
Publisher: Elsevier BV
Date: 02-2021
Publisher: American Chemical Society (ACS)
Date: 17-06-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 22-09-2014
DOI: 10.1039/C4AN01391C
Abstract: Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is ideally suited for the high-throughput analysis of small molecules in bodily fluids (e.g. saliva, urine, and blood plasma). A key application for this technique is the testing of drug consumption in the context of workplace, roadside, athlete sports and anti-addictive drug compliance. Here, we show that vertically-aligned ordered silicon nanopillar (SiNP) arrays fabricated using nanosphere lithography followed by metal-assisted chemical etching (MACE) are suitable substrates for the SALDI-MS detection of methadone and small peptides. Porosity, length and diameter are fabrication parameters that we have explored here in order to optimize analytical performance. We demonstrate the quantitative analysis of methadone in MilliQ water down to 32 ng mL(-1). Finally, the capability of SiNP arrays to facilitate the detection of methadone in clinical s les is also demonstrated.
Publisher: American Chemical Society (ACS)
Date: 25-09-2017
Abstract: In order to address the issue of pathogenic bacterial colonization of diabetic wounds, a more direct and robust approach is required, which relies on a physical form of bacterial destruction in addition to the conventional biochemical approach (i.e., antibiotics). Targeted bacterial destruction through the use of photothermally active nanomaterials has recently come into the spotlight as a viable approach to solving the rising problem of antibiotic resistant microorganisms. Materials with high absorption coefficients in the near-infrared (NIR) region of the electromagnetic spectrum show promise as alternative antibacterial therapeutic agents, since they preclude the development of bacterial resistance and can be activated on demand. Here were report on a novel approach for the fabrication of gold nanoparticle decorated porous silicon nanopillars with tunable geometry that demonstrate excellent photothermal conversion properties when irradiated with a 808 nm laser. These photothermal antibacterial properties are demonstrated in vitro against the Gram-positive bacteria Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli). Results show a reduction in bacterial viability of up to 99% after 10 min of laser irradiation. We also show an increase in antibacterial performance after modifying the nanopillars with S. aureus targeting antibodies causing up to a 10-fold increase in bactericidal efficiency compared to E. coli. In contrast, the nanomaterial resulted in minimal disruption of metabolic processes in human foreskin fibroblasts (HFF) after an equivalent period of irradiation.
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D2LC00692H
Abstract: Controllable constrictions in PDMS microchannels were achieved by applying external mechanical force to facilitate cell membrane permeation and intracellular delivery.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6RA13734B
Abstract: Silicon nanowires fabricated through Ag-assisted chemical etching were found to be effective bacterial-traps with strong antibacterial properties resulting from Ag-nanoclusters.
Publisher: Wiley
Date: 15-12-2014
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 04-2023
Publisher: IOP Publishing
Date: 18-01-2016
DOI: 10.1088/0957-4484/27/7/075301
Abstract: We present an elegant route for the fabrication of ordered arrays of vertically-aligned silicon nanowires with tunable geometry at controlled locations on a silicon wafer. A monolayer of transparent microspheres convectively assembled onto a gold-coated silicon wafer acts as a microlens array. Irradiation with a single nanosecond laser pulse removes the gold beneath each focusing microsphere, leaving behind a hexagonal pattern of holes in the gold layer. Owing to the near-field effects, the diameter of the holes can be at least five times smaller than the laser wavelength. The patterned gold layer is used as catalyst in a metal-assisted chemical etching to produce an array of vertically-aligned silicon nanowires. This approach combines the advantages of direct laser writing with the benefits of parallel laser processing, yielding nanowire arrays with controlled geometry at predefined locations on the silicon surface. The fabricated VA-SiNW arrays can effectively transfect human cells with a plasmid encoding for green fluorescent protein.
Publisher: Wiley
Date: 09-2016
Publisher: American Chemical Society (ACS)
Date: 27-03-2020
Publisher: Wiley
Date: 13-10-2015
Publisher: Elsevier BV
Date: 07-2021
Publisher: American Chemical Society (ACS)
Date: 04-01-2022
Abstract: Mass spectrometry of intact nanoparticles and viruses can serve as a potent characterization tool for material science and biophysics. Inaccessible by widespread commercial techniques, the mass of single nanoparticles and viruses (>10MDa) can be readily measured by nanoelectromechanical systems (NEMS)-based mass spectrometry, where charged and isolated analyte particles are generated by electrospray ionization (ESI) in air and transported onto the NEMS resonator for capture and detection. However, the applicability of NEMS as a practical solution is hindered by their miniscule surface area, which results in poor limit-of-detection and low capture efficiency values. Another hindrance is the necessity to house the NEMS inside complex vacuum systems, which is required in part to focus analytes toward the miniscule detection surface of the NEMS. Here, we overcome both limitations by integrating an ion lens onto the NEMS chip. The ion lens is composed of a polymer layer, which charges up by receiving part of the ions incoming from the ESI tip and consequently starts to focus the analytes toward an open window aligned with the active area of the NEMS electrostatically. With this integrated system, we have detected the mass of gold and polystyrene nanoparticles under ambient conditions and with two orders-of-magnitude improvement in capture efficiency compared to the state-of-the-art. We then applied this technology to obtain the mass spectrum of SARS-CoV-2 and BoHV-1 virions. With the increase in analytical throughput, the simplicity of the overall setup, and the operation capability under ambient conditions, the technique demonstrates that NEMS mass spectrometry can be deployed for mass detection of engineered nanoparticles and biological s les efficiently.
Start Date: 03-2015
End Date: 05-2018
Amount: $295,900.00
Funder: Australian Research Council
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