ORCID Profile
0000-0001-7667-3648
Current Organisation
University of South Australia
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Publisher: The Optical Society
Date: 13-02-2012
DOI: 10.1364/OE.20.004968
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 09-2017
Publisher: IEEE
Date: 09-2014
Publisher: The Optical Society
Date: 07-08-2013
DOI: 10.1364/OME.3.001242
Publisher: IEEE
Date: 08-2015
Publisher: Elsevier BV
Date: 02-2023
DOI: 10.1016/J.PATHOL.2022.08.001
Abstract: Diagnosis and assessment of patients with prostate cancer is dependent on accurate interpretation and grading of histopathology. However, morphology does not necessarily reflect the complex biological changes occurring in prostate cancer disease progression, and current biomarkers have demonstrated limited clinical utility in patient assessment. This study aimed to develop biomarkers that accurately define prostate cancer biology by distinguishing specific pathological features that enable reliable interpretation of pathology for accurate Gleason grading of patients. Online gene expression databases were interrogated and a pathogenic pathway for prostate cancer was identified. The protein expression of key genes in the pathway, including adaptor protein containing a pleckstrin homology (PH) domain, phosphotyrosine-binding (PTB) domain, and leucine zipper motif 1 (Appl1), Sortilin and Syndecan-1, was examined by immunohistochemistry (IHC) in a pilot study of 29 patients with prostate cancer, using monoclonal antibodies designed against unique epitopes. Appl1, Sortilin, and Syndecan-1 expression was first assessed in a tissue microarray cohort of 112 patient s les, demonstrating that the monoclonal antibodies clearly illustrate gland morphologies. To determine the impact of a novel IHC-assisted interpretation (the utility of Appl1, Sortilin, and Syndecan-1 labelling as a panel) of Gleason grading, versus standard haematoxylin and eosin (H&E) Gleason grade assignment, a radical prostatectomy s le cohort comprising 114 patients was assessed. In comparison to H&E, the utility of the biomarker panel reduced subjectivity in interpretation of prostate cancer tissue morphology and improved the reliability of pathology assessment, resulting in Gleason grade redistribution for 41% of patient s les. Importantly, for equivocal IHC-assisted labelling and H&E staining results, the cancer morphology interpretation could be more accurately applied upon re-review of the H&E tissue sections. This study addresses a key issue in the field of prostate cancer pathology by presenting a novel combination of three biomarkers and has the potential to transform clinical pathology practice by standardising the interpretation of the tissue morphology.
Publisher: IEEE
Date: 08-2015
Publisher: AIP Publishing
Date: 27-09-2016
DOI: 10.1063/1.4963141
Abstract: Efficient methods to modulate terahertz (THz) light are essential for realizing rapid THz imaging and communication applications. Here we report a novel THz modulator which utilizes the evanescent wave in a total internal reflection setup coupled with a conductive interface to enhance the attenuation efficiency of THz light. This approach makes it possible to achieve close to 100% modulation with a small interface conductivity of 12 mS. The frequency dependence of this technique is linked to the optical properties of the materials: a material with close to frequency independent conductivity that is also controllable will result in an achromatic modulation response, and the device performance can be optimized further by tuning the internal reflection angle. In this work, we focus on applying the technique in the terahertz frequency range. Using an LED array with a pump intensity of 475 mW/cm2 to produce carriers in a silicon wafer, we have achieved a modulation depth of up to 99.9% in a broad frequency range of 0.1 THz–0.8 THz. The required pumping power for the generation of the required free carriers is low because the sheet conductivity needed is far less than required for traditional transmission techniques. Consequently, the device can be modulated by an LED making it a very practical, low cost, and scalable solution for THz modulation.
Publisher: The Optical Society
Date: 07-03-2011
DOI: 10.1364/OE.19.005327
Publisher: IEEE
Date: 09-2016
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 03-2013
Publisher: Wiley
Date: 02-11-2016
Abstract: We use terahertz imaging to measure four human skin scars in vivo. Clear contrast between the refractive index of the scar and surrounding tissue was observed for all of the scars, despite some being difficult to see with the naked eye. Additionally, we monitored the healing process of a hypertrophic scar. We found that the contrast in the absorption coefficient became less prominent after a few months post-injury, but that the contrast in the refractive index was still significant even months post-injury. Our results demonstrate the capability of terahertz imaging to quantitatively measure subtle changes in skin properties and this may be useful for improving scar treatment and management.
Publisher: IEEE
Date: 09-2016
Publisher: The Optical Society
Date: 26-10-2016
DOI: 10.1364/BOE.7.004711
Publisher: MDPI AG
Date: 16-06-2023
Abstract: Gleason scoring is used within a five-tier risk stratification system to guide therapeutic decisions for patients with prostate cancer. This study aimed to compare the predictive performance of routine H& E or biomarker-assisted ISUP (International Society of Urological Pathology) grade grouping for assessing the risk of biochemical recurrence (BCR) and clinical recurrence (CR) in patients with prostate cancer. This retrospective study was an assessment of 114 men with prostate cancer who provided radical prostatectomy s les to the Australian Prostate Cancer Bioresource between 2006 and 2014. The prediction of CR was the primary outcome (median time to CR 79.8 months), and BCR was assessed as a secondary outcome (median time to BCR 41.7 months). The associations of (1) H& E ISUP grade groups and (2) modified ISUP grade groups informed by the Appl1, Sortilin and Syndecan-1 immunohistochemistry (IHC) labelling were modelled with BCR and CR using Cox proportional hazard approaches. IHC-assisted grading was more predictive than H& E for BCR (C-statistic 0.63 vs. 0.59) and CR (C-statistic 0.71 vs. 0.66). On adjusted analysis, IHC-assisted ISUP grading was independently associated with both outcome measures. IHC-assisted ISUP grading using the biomarker panel was an independent predictor of in idual BCR and CR. Prospective studies are needed to further validate this biomarker technology and to define BCR and CR associations in real-world cohorts.
Publisher: IOP Publishing
Date: 28-08-2014
Publisher: IEEE
Date: 09-2012
Publisher: IEEE
Date: 09-2012
Publisher: IEEE
Date: 09-2016
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 03-2018
Publisher: Springer Science and Business Media LLC
Date: 2014
Publisher: MDPI AG
Date: 23-12-2021
Abstract: Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability (2) amenability of biological models to fluorescence microscopy and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.
Publisher: IEEE
Date: 09-2010
Publisher: MDPI AG
Date: 19-09-2023
Publisher: IEEE
Date: 09-2014
Publisher: IEEE
Date: 08-2015
Publisher: IEEE
Date: 09-2014
Publisher: American Chemical Society (ACS)
Date: 13-03-2015
Publisher: Springer Science and Business Media LLC
Date: 18-08-2023
DOI: 10.1038/S41598-023-40347-7
Abstract: Prostate cancer (PCa) development and progression relies on the programming of glucose and lipid metabolism, and this involves alterations in androgen receptor expression and signalling. Defining the molecular mechanism that underpins this metabolic programming will have direct significance for patients with PCa who have a poor prognosis. Here we show that there is a dynamic balance between sortilin and syndecan-1, that reports on different metabolic phenotypes. Using tissue microarrays, we demonstrated by immunohistochemistry that sortilin was highly expressed in low-grade cancer, while syndecan-1 was upregulated in high-grade disease. Mechanistic studies in prostate cell lines revealed that in androgen-sensitive LNCaP cells, sortilin enhanced glucose metabolism by regulating GLUT1 and GLUT4, while binding progranulin and lipoprotein lipase (LPL) to limit lipid metabolism. In contrast, in androgen-insensitive PC3 cells, syndecan-1 was upregulated, interacted with LPL and colocalised with β 3 integrin to promote lipid metabolism. In addition, androgen-deprived LNCaP cells had decreased expression of sortilin and reduced glucose-metabolism, but increased syndecan-1 expression, facilitating interactions with LPL and possibly β 3 integrin. We report a hitherto unappreciated molecular mechanism for PCa, which may have significance for disease progression and how androgen-deprivation therapy might promote castration-resistant PCa.
Publisher: IEEE
Date: 09-2012
Publisher: IEEE
Date: 09-2009
Publisher: SPIE
Date: 21-12-2008
DOI: 10.1117/12.759825
Publisher: IOP Publishing
Date: 13-03-2015
DOI: 10.1088/0031-9155/60/7/2703
Abstract: S le dehydration has traditionally been a challenging problem in ex vivo terahertz biomedical experiments as water content changes significantly affect the terahertz properties and can diminish important contrast features. In this paper, we propose a novel method to prevent s le dehydration using gelatin embedding. By looking at terahertz image data and calculating the optical properties of the gelatin-embedded s le, we find that our method successfully preserves the s le for at least 35 h, both for imaging and spectroscopy. Our novel preservation method demonstrates for the first time the capability to simultaneously maintain s le structural integrity and prevent dehydration at room temperature. This is particularly relevant for terahertz studies of freshly excised tissues but could be beneficial for other imaging and spectroscopy techniques.
Publisher: Elsevier BV
Date: 10-2023
Publisher: SPIE
Date: 27-12-2007
DOI: 10.1117/12.695716
Publisher: Elsevier BV
Date: 09-2023
Publisher: IEEE
Date: 09-2008
Publisher: The Optical Society
Date: 31-05-2016
DOI: 10.1364/PRJ.4.000A29
Publisher: Springer Science and Business Media LLC
Date: 29-09-2023
Publisher: Wiley
Date: 27-12-2016
Publisher: The Optical Society
Date: 30-01-2013
DOI: 10.1364/OE.21.002875
Publisher: IEEE
Date: 09-2006
Publisher: Springer Science and Business Media LLC
Date: 07-12-2022
Publisher: IEEE
Date: 09-2009
Publisher: AIP Publishing
Date: 06-02-2012
DOI: 10.1063/1.3665180
Abstract: In this work, we characterize the electromagnetic properties of polydimethylsiloxane (PDMS) and use this as a free-standing substrate for the realization of flexible fishnet metamaterials at terahertz frequencies. Across the 0.2–2.5 THz band, the refractive index and absorption coefficient of PDMS are estimated as 1.55 and 0–22 cm−1, respectively. Electromagnetic modeling, multi-layer flexible electronics microfabrication, and terahertz time-domain spectroscopy are used in the design, fabrication, and characterization of the metamaterials, respectively. The properties of PDMS add a degree of freedom to terahertz metamaterials, with the potential for tuning by elastic deformation or integrated microfluidics.
Publisher: The Optical Society
Date: 11-06-2013
DOI: 10.1364/OL.38.002104
Publisher: AIP Publishing
Date: 25-03-2013
DOI: 10.1063/1.4773238
Abstract: Electromagnetic device design and flexible electronics fabrication are combined to demonstrate mechanically tunable metamaterials operating at terahertz frequencies. Each metamaterial comprises a planar array of resonators on a highly elastic polydimethylsiloxane substrate. The resonance of the metamaterials is controllable through substrate deformation. Applying a stretching force to the substrate changes the inter-cell capacitance and hence the resonance frequency of the resonators. In the experiment, greater than 8% of the tuning range is achieved with good repeatability over several stretching-relaxing cycles. This study promises applications in remote strain sensing and other controllable metamaterial-based devices.
Publisher: American Chemical Society (ACS)
Date: 11-06-2014
DOI: 10.1021/PH500110T
Publisher: IEEE
Date: 09-2009
Publisher: Springer Science and Business Media LLC
Date: 14-09-2023
Publisher: Research Square Platform LLC
Date: 27-03-2023
DOI: 10.21203/RS.3.RS-2693853/V1
Abstract: Prostate cancer (PCa) development and progression relies on the programming of glucose and lipid metabolism, and this involves alterations in androgen receptor expression and signalling. Defining the molecular mechanism that underpins this metabolic programming will have direct significance for patients with PCa who have a poor prognosis. Here we show that there is a dynamic balance between sortilin and syndecan-1, that reports on different metabolic phenotypes. Using tissue microarrays, we demonstrated by immunohistochemistry that sortilin was highly expressed in low-grade cancer, while syndecan-1 was upregulated in high-grade disease. Mechanistic studies in prostate cell lines (selected to match the biomarker phenotypes in tissue from PCa patients) revealed that in androgen-sensitive LNCaP cells, sortilin enhanced glucose metabolism by regulating GLUT1 and GLUT4, while binding progranulin and lipoprotein lipase (LPL) to limit lipid metabolism. In contrast, in androgen-insensitive PC3 cells, syndecan-1 was upregulated, interacted with LPL and colocalised with β 3 integrin to promote lipid metabolism. In addition, androgen-deprived LNCaP cells had decreased expression of sortilin and reduced glucose-metabolism, but increased syndecan-1 expression, facilitating interactions with LPL and possibly β 3 integrin. We report a hitherto unappreciated molecular mechanism for PCa, which has significance for disease progression and how androgen-deprivation therapy may promote castration-resistant PCa.
No related grants have been discovered for Benjamin Ung.