ORCID Profile
0000-0002-8088-1981
Current Organisation
University of Tasmania
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Publisher: Wiley
Date: 16-03-2020
Publisher: MDPI AG
Date: 30-01-2019
DOI: 10.3390/BIOS9010019
Abstract: On-site therapeutic drug monitoring (TDM) is important for providing a quick and accurate dosing to patients in order to improve efficacy and minimize toxicity. Aminoglycosides such as amikacin, gentamicin, and tobramycin are important antibiotics that have been commonly used to treat infections of chronic bacterial infections in the urinary tract, lung, and heart. However, these aminoglycosides can lead to vestibular and auditory dysfunction. Therefore, TDM of aminoglycosides is important due to their ototoxicity and nephrotoxicity. Here, we have developed a hot embossed poly (methyl methacrylate) (PMMA) microfluidic device featuring an electrokinetic size and mobility trap (SMT) to purify, concentrate, and separate the aminoglycoside antibiotic drugs amikacin, gentamicin, and tobramycin. These drugs were separated successfully from whole blood within 3 min, with 30-fold lower detection limits compared to a standard pinched injection. The limit of detections (LOD) were 3.75 µg/mL for gentamicin, 8.53 µg/mL for amikacin, and 6.00 µg/mL for tobramycin. These are sufficient to cover the therapeutic range for treating sepsis of 6–10 μg/mL gentamicin and tobramycin and 12–20 μg/mL of amikacin. The device is simple and could be mass produced via embossing or injection molding approaches.
Publisher: American Chemical Society (ACS)
Date: 08-09-2020
Publisher: Wiley
Date: 25-05-2020
Publisher: Elsevier BV
Date: 07-2019
DOI: 10.1016/J.CHROMA.2019.03.019
Abstract: The Copper (II) complex of β-cyclodextrin (β-CD), β-CDCu
Publisher: Wiley
Date: 28-08-2020
Publisher: Institution of Engineering and Technology
Date: 19-07-2016
Publisher: Elsevier BV
Date: 07-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C3AY42106F
Abstract: A microemulsion electrokinetic chromatography (MEEKC) method was developed for the simultaneous determination of three curcuminoids to avoid their alkaline degradation during capillary electrophoresis (CE) analysis with alkaline buffers.
Publisher: American Chemical Society (ACS)
Date: 25-07-2016
DOI: 10.1021/ACS.ANALCHEM.6B02096
Abstract: A microfluidic device with two nanoporous membranes was developed to seamlessly integrate s le preparation and electrophoretic separation of proteins. The device was fabricated by sandwiching two nanoporous polycarbonate track etched (PCTE) membranes with differently sized nanopores between PDMS slabs containing embedded microchannels. The first membrane contained larger (100 nm) pores and served as an initial filter to screen out particles, cells and larger proteins. The second membrane contained smaller pores (10 nm) which facilitated transport of inorganic ions and small organic molecules, but not proteins. The sequential combination of these two membranes allows proteins to be concentrated and purified simultaneously. The device was used for the s le-in/answer-out quantification of albumin in human urine within 2.5 min with an improvement in sensitivity of 500 fold compared to a normal pinched injection using fluorescence detection. The linear range of was 0-100 μg mL(-1), with a LOD of 1.5 μg mL(-1) covering the diagnostic level of microalbuminuria of 30 μg mL(-1). The presented device, which is simple to make and use, provides a quantitative alternative for point-of-care detection of proteins, as demonstrated through its application to albumin in urine for the diagnoisis of (micro)albuminuria.
Publisher: Elsevier BV
Date: 06-2019
DOI: 10.1016/J.CHROMA.2019.02.023
Abstract: A concentric electromembrane extraction preconcentration device was designed and fabricated using fused deposition modelling 3D printing. The device consisted of a hemispherical electrode s le vial printed from a filament of conductive polylactic acid (PLA), into which sat a smaller hemispherical 3D printed porous membrane acceptor vial. Application of voltage between a point-electrode placed in the center of 20 μL solution inside the acceptor vial and the electrode vial containing 1 mL of s le, enabled anion migration through the 3D printed porous material into the acceptor solution. Electromembrane extraction was proved using fluorescein for imaging of the extraction process, with preconcentration rates of 0.833 μM/sec at 120 V with close to 95% recovery. The performance of the fabricated porous 3D printed device was evaluated for the preconcentration of anions from water prior to capillary electrophoresis detection. Preconcentration factors ranging between 36-44 were obtained for chloride, nitrate, perchlorate and sulfate, while a lower performance was observed for weaker acids as fluoride and phosphate (3-4). The limit of detection (LOD) was determined to be 0.16 μM, 0.18 μM and 0.18 μM for NO
Publisher: Springer Science and Business Media LLC
Date: 12-07-2013
Publisher: American Chemical Society (ACS)
Date: 17-09-2018
DOI: 10.1021/ACS.ANALCHEM.8B02893
Abstract: Multimaterial 3D printing facilitates the rapid production of complex devices with integrated materials of varying properties and functionality. Herein, multimaterial fused deposition modeling (MM-FDM) 3D printing was applied to the fabrication of low-cost passive s ler devices with integrated porous membranes. Using MM-FDM 3D printing, the device body was produced using black polylactic acid, with Poro-Lay Lay-Felt filament used for the printing of the integrated porous membranes (rubber-elastomeric polymer, porous after removal of a water-soluble poly(vinyl alcohol) component). The resulting device consisted of two interlocking circular frames, each containing the integrated membrane, which could be efficiently sealed together without the need for additional O-rings, and prevented loss of enclosed microparticulate sorbent. Scanning electron microscopy (SEM) analysis of the purified composite filament confirmed the porous properties of the material, an average pore size of ∼30 nm. The printed passive s lers with various membrane thicknesses, including 0.5, 1.0, and 1.5 mm, were evaluated for their ability to facilitate the extraction of atrazine as the model solute onto the internal sorbent, under standard conditions. Gas chromatography-mass spectrometry was used to determine the uptake of atrazine by the device from standard water s les and also to evaluate any chemical leaching from the printed materials. The s ler with 0.5 mm thick membrane showed the best performance with 87% depletion and a s ling rate of 0.19 Ld
Publisher: Elsevier BV
Date: 11-2012
DOI: 10.1016/J.TALANTA.2012.10.015
Abstract: In the present study, a rapid and repeatable microemulsion electrokinetic chromatography (MEEKC) method was developed for the simultaneous determination of three isomers (α-, β- and γ-asarone) in Acorus tatarinowii by using ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF(6)) as oil phase. Experimental parameters including the microemulsion compositions (concentrations of surfactant, co-surfactant and oil phase), pH, concentration of borate buffer, capillary temperature and voltage were intensively investigated. Finally, the main compounds in the methanol extract of A. tatarinowii were well separated within 11 min using a running buffer composed of 40 mmol/L sodium dodecyl sulfonate (SDS), 2.0 mol/L n-propanol, 8 mmol/L [BMIM]PF(6) in 10 mmol/L borate buffer of pH 9.5. The developed method was applied to determine the contents of α-, β- and γ-asarone in A. tatarinowii from five different producing areas in China (Anhui, Hebei, Sichuan, Zhejiang and Chongqing). The results indicated that the contents of three asarones are quite different in the investigated A. tatarinowii s les. On the other hand, the MEEKC with ionic liquid as oil phase should be a promising method for the analysis of volatile components especially isomers in medicinal herbs.
Publisher: Elsevier BV
Date: 03-2021
Publisher: Elsevier BV
Date: 10-2018
No related grants have been discovered for Feng Li.