ORCID Profile
0000-0002-4781-5202
Current Organisation
Murdoch University
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Elsevier BV
Date: 08-2018
DOI: 10.1016/J.FORSCIINT.2018.05.021
Abstract: In sexual assault cases and more specifically those involving childhood sexual abuse (CSA), victims may have had their potentially semen-stained clothing washed multiple times before a criminal investigation commences. Although it has been previously demonstrated that spermatozoa persist on cotton clothing following a single wash cycle, items of clothing washed multiple times are not routinely examined in these cases because of the assumption that the laundering process would have removed all seminal fluid and spermatozoa. The aim of this study was to examine the persistence of seminal fluid and spermatozoa on a range of fabric types including cotton, nylon, terry towel (100% cotton), polyester fleece, satin and lace which were laundered up to six times. Three techniques were used for the detection of seminal fluid and spermatozoa: an alternative light source, acid phosphatase test and microscopy. The study demonstrated that spermatozoa persisted on cotton and terry towel following six wash cycles. This data emphasises the need to recover and examine items of clothing and bedding of victims for semen, even if the item has been washed multiple times.
Publisher: Informa UK Limited
Date: 18-03-2020
Publisher: Elsevier BV
Date: 05-2019
DOI: 10.1016/J.FORSCIINT.2019.02.048
Abstract: We report the preliminary observations of the peptide content of decomposition fluid produced under controlled laboratory conditions and in the absence of a soil matrix. Four domestic pig (Sus scrofa domesticus) cadavers were used to model human decomposition over a four-week trial period physical characteristics were recorded and the peptide components of decomposition fluid was analysed using high performance liquid chromatography-time of flight mass spectrometry. Preliminary data analysis indicated that a range of peptides were consistently detected across the course of the trial period and 27 of these were common to all four cadavers 22 originating from haemoglobin. The peptides associated with haemoglobin subunit alpha and beta displayed a breakdown pattern that remained consistent for all cadavers for the duration of the trial. Though identification of peptides during decomposition has potential for estimating the time since death, quantification of selected peptides is likely to be essential to identify time-dependent trends.
Publisher: Elsevier BV
Date: 10-2019
DOI: 10.1016/J.FORSCIINT.2019.109957
Abstract: We report the peptide content of decomposition fluid produced under field-based conditions and in the absence of a soil matrix. Sixteen domestic pig (Sus scrofa domesticus) cadavers were used to model human decomposition in trials conducted in the summer and winter months in Western Australia. Physical characteristics were recorded and the peptide components of decomposition fluid were analysed using high performance liquid chromatography-time of flight mass spectrometry. A range of peptides was consistently detected in both summer and winter. Thirty seven peptides were common to both trials 22 originating from haemoglobin subunit beta, 1 from haemoglobin subunit alpha, 4 from beta-enolase, and 2 from creatine kinase. In agreement with our previous findings, 13 peptides occurred consistently, regardless of trial conditions. Degradation patterns for haemoglobin subunits alpha and beta in summer and winter were similar when expressed in ADD and when adjusted for differences in temperature. The consistent identification of several protein-specific peptides generated during decomposition trials conducted under different temperature and rainfall regimes suggests that quantitative peptide analysis may be useful in estimating time since death.
No related grants have been discovered for Ashley-N’Dene Nolan.