ORCID Profile
0000-0002-2556-0528
Current Organisations
University of Melbourne
,
Deakin University
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Springer Science and Business Media LLC
Date: 23-01-2016
DOI: 10.1007/S11130-016-0527-8
Abstract: Ocimum basilicum, a member of the family Lamiaceae, is a rich source of polyphenolics that have antioxidant properties. The present study describes the development and application of an online HPLC-coupled acidic potassium permanganate chemiluminescence assay for the qualitative and quantitative assessment of antioxidants in three cultivars of O. basilicum grown under greenhouse conditions. The chemiluminescence based assay was found to be a sensitive and efficient method for assessment of total and in idual compound antioxidant potential. Leaves, flowers and roots were found to be rich reserves of the antioxidant compounds which showed intense chemiluminescence signals. The polyphenolics such as rosmarinic, chicoric, caffeic, p-coumaric, m-coumaric and ferulic acids showed antioxidant activity. Further, rosmarinic acid was found to be the major antioxidant component in water-ethanol extracts. The highest levels of rosmarinic acid was found in the leaves and roots of cultivars "holy green" (14.37 11.52 mM/100 g DW respectively) followed by "red rubin" (10.02 10.75 mM/100 g DW respectively) and "subja" (6.59 4.97 mM/100 g DW respectively). The sensitivity, efficiency and ease of use of the chemiluminescence based assay should now be considered for its use as a primary method for the identification and quantification of antioxidants in plant extracts.
Publisher: Springer Science and Business Media LLC
Date: 02-08-2016
DOI: 10.1007/S00572-016-0721-4
Abstract: Arbuscular mycorrhiza is a symbiotic association formed between plant roots and soil borne fungi that alter and at times improve the production of secondary metabolites. Detailed information is available on mycorrhizal development and its influence on plants grown under various edapho-climatic conditions, however, very little is known about their influence on transformed roots that are rich reserves of secondary metabolites. This raises the question of how mycorrhizal colonization progresses in transformed roots grown in vitro and whether the mycorrhizal fungus presence influences the production of secondary metabolites. To fully understand mycorrhizal ontogenesis and its effect on root morphology, root biomass, total phenolics, rosmarinic acid, caffeic acid and antioxidant production under in vitro conditions, a co-culture was developed between three Agrobacterium rhizogenes-derived, elite-transformed root lines of Ocimum basilicum and Rhizophagus irregularis. We found that mycorrhizal ontogenesis in transformed roots was similar to mycorrhizal roots obtained from an in planta system. Mycorrhizal establishment was also found to be transformed root line-specific. Colonization of transformed roots increased the concentration of rosmarinic acid, caffeic acid and antioxidant production while no effect was observed on root morphological traits and biomass. Enhancement of total phenolics and rosmarinic acid in the three mycorrhizal transformed root lines was found to be transformed root line-specific and age dependent. We reveal the potential of R. irregularis as a biotic elicitor in vitro and propose its incorporation into commercial in vitro secondary metabolite production via transformed roots.
Publisher: Elsevier BV
Date: 07-2014
Publisher: Oxford University Press (OUP)
Date: 28-04-2003
DOI: 10.1093/JXB/ERG174
Publisher: Wiley
Date: 1983
Publisher: MDPI AG
Date: 12-08-2022
DOI: 10.3390/PATHOGENS11080907
Abstract: The fungus Gnomoniopsis smithogilvyi is a significant threat to the production of sweet chestnut (Castanea sativa) nuts in Australia and worldwide. The pathogen causes nut rot, which leads to substantial production losses. Early and accurate diagnosis of the disease is essential to delineate and implement control strategies. A specific and sensitive multiplex PCR was developed based on the lification of three barcode sequences of G. smithogilvyi. The assay reliability was enhanced by including the lification of a host gene as an internal control. Primers were thoroughly evaluated in silico before assessing them in vitro. Primer annealing temperature and concentration were optimised to enhance the assay sensitivity and specificity. The assay detection limit ranged between 0.1 and 1.0 pg (5 and 50 fg/μL) of genomic DNA per reaction. No cross-reactivity was observed with genomic DNA from closely and distantly related fungal species. We also characterised Australian G. smithogilvyi isolates phenotypically and genotypically and found significant differences in morphologic and virulence traits of the isolates. An understanding of the virulence of G. smithogilvyi and the availability of a reliable and accurate diagnostic technique will enable earlier detection of the pathogen, which will contribute to effective control strategies for the disease.
Publisher: Springer Science and Business Media LLC
Date: 13-12-2015
Publisher: Oxford University Press (OUP)
Date: 08-1986
DOI: 10.1104/PP.81.4.1103
Publisher: American Chemical Society (ACS)
Date: 07-03-2022
Publisher: American Chemical Society (ACS)
Date: 22-07-2015
DOI: 10.1021/ACS.LANGMUIR.5B01637
Abstract: The growth mechanism and kinetics of mesoporous silica nanoparticles (MSNs) were investigated for the first time by using a synchrotron time-resolved small-angle X-ray scattering (SAXS) analysis. The synchrotron SAXS offers unsurpassed time resolution and the ability to detect structural changes of nanometer sized objects, which are beneficial for the understanding of the growth mechanism of small MSNs (∼20 nm). The Porod invariant was used to quantify the conversion of tetraethyl orthosilicate (TEOS) in silica during MSN formation, and the growth kinetics were investigated at different solution pH and temperature through calculating the scattering invariant as a function of reaction time. The growth of MSNs was found to be accelerated at high temperature and high pH, resulting in a higher rate of silica formation. Modeling SAXS data of micelles, where a well-defined electrostatic interaction is assumed, determines the size and shape of hexadecyltrimethylammonium bromide (CTAB) micelles before and after the addition of TEOS. The results suggested that the micelle size increases and the micelle shape changes from ellipsoid to spherical, which might be attributed to the solubilization of TEOS in the hydrophobic core of CTAB micelles. A new "swelling-shrinking" mechanism is proposed. The mechanism provides new insights into understanding MSN growth for the formation of functional mesoporous materials exhibiting controlled morphologies. The SAXS analyses were correlated to the structure of CTAB micelles and chemical reaction of TEOS. This study has provided critical information to an understanding of the growth kinetics and mechanism of MSNs.
Publisher: CSIRO Publishing
Date: 2011
DOI: 10.1071/FP11026
Abstract: Microarray analysis was used to investigate changes in host gene expression during the primary stages of the interaction between the susceptible plant Arabidopsis thaliana (L.) Heynh ecotype Col-0 and the biotrophic pathogen Plasmodiophora brassicae Woronin. Analyses were conducted at 4, 7 and 10 days after inoculation (DAI) and revealed significant induction or suppression of a relatively low number of genes in a range of functional categories. At 4 DAI, there was induced expression of several genes known to be critical for pathogen recognition and signal transduction in other resistant host–pathogen interactions. As the pathogen further colonised root tissue and progressed through the primary plasmodium stage to production of zoosporangia at 7 and 10 DAI, respectively, fewer genes showed changes in expression. The microarray results were validated by examining a subset of induced genes at 4 DAI by quantitative real-time reverse transcriptase PCR (RT-qPCR) analysis all of which correlated positively with the microarray data. The two A. thaliana mutants jar1 and coiI tested were found to be susceptible to P. brassicae. The involvement of defence-related hormones in the interaction was further investigated and the findings indicate that addition of salicylic acid can suppress clubroot disease in A. thaliana plants.
Publisher: Elsevier BV
Date: 08-2018
DOI: 10.1016/J.PLAPHY.2018.05.031
Abstract: Cell ision is a fundamental biological process governed by molecular networks that are initiated in the apical meristems of plants. l-ascorbic acid (AsA) commonly known as vitamin C is a crucial molecular modulator involved in cell proliferation. In this study, we used AsA application to Arabidopsis and four AsA pathway mutants to investigate the influence of AsA on the root apical meristem (RAM) and root growth. Treatment of seeds of wild-type Col-0 with AsA prior to sowing showed a significant increase in the activity of cell ision of the RAM, root growth rate and root length when compared with untreated seeds. Seedlings of the AsA pathway mutant vtc1-1 showed a significant reduction in the level of AsA and a significant increase in the number of quiescent cells in the RAM when compared with Col-0. Cell proliferation was reduced in the AsA pathway mutants vtc1-1, dhar1, vtc5-1, apx1, respectively, however, root growth decreased significantly only in vtc1-1 when compared with Col-0. In addition, hydrogen peroxide (H
Publisher: Elsevier BV
Date: 07-2008
Publisher: Oxford University Press (OUP)
Date: 09-1989
DOI: 10.1104/PP.91.1.23
Publisher: Wiley
Date: 04-2004
Publisher: CSIRO Publishing
Date: 2008
DOI: 10.1071/BT07159
Abstract: Phytophthora cinnamomi continues to cause devastating disease in Australian native vegetation and consequently the disease is listed by the Federal Government as a process that is threatening Australia’s bio ersity. Although several advances have been made in our understanding of how this soil-borne pathogen interacts with plants and of how we may tackle it in natural systems, our ability to control the disease is limited. The pathogen occurs widely across Australia but the severity of its impact is most evident within ecological communities of the south-west and south-east of the country. A regional impact summary for all states and territories shows the pathogen to be the cause of serious disease in numerous species, a significant number of which are rare and threatened. Many genera of endemic taxa have a high proportion of susceptible species including the iconic genera Banksia, Epacris and Xanthorrhoea. Long-term studies in Victoria have shown limited but probably unsustainable recovery of susceptible vegetation, given current management practices. Management of the disease in conservation reserves is reliant on hygiene, the use of chemicals and restriction of access, and has had only limited effectiveness and not provided complete control. The deleterious impacts of the disease on faunal habitat are reasonably well documented and demonstrate loss of in idual animal species and changes in population structure and species abundance. Few plant species are known to be resistant to P. cinnamomi however, investigations over several years have discovered the mechanisms by which some plants are able to survive infection, including the activation of defence-related genes and signalling pathways, the reinforcement of cell walls and accumulation of toxic metabolites. Manipulation of resistance and resistance-related mechanisms may provide avenues for protection against disease in otherwise susceptible species. Despite the advances made in Phytophthora research in Australia during the past 40 years, there is still much to be done to give land managers the resources to combat this disease. Recent State and Federal initiatives offer the prospect of a growing and broader awareness of the disease and its associated impacts. However, awareness must be translated into action as time is running out for the large number of susceptible, and potentially susceptible, species within vulnerable Australian ecological communities.
Publisher: Elsevier BV
Date: 08-1989
Publisher: Elsevier
Date: 2006
Publisher: American Chemical Society (ACS)
Date: 04-03-2013
DOI: 10.1021/AM303208T
Abstract: Agrochemical spray formulations applied to plants are often mixed with surfactants that facilitate delivery of the active ingredient. However, surfactants cause phytotoxicity and off-target effects in the environment. We propose the use of nanostructured liquid crystalline particles (NLCP) as an alternative to surfactant-based agrochemical delivery. For this, we have compared the application of commercial surfactants, di (2-ethylhexyl) sulfosuccinate and alkyl dimethyl betaine, with NLCP made from phytantriol, at concentrations of 0.1%, 1% and 5% on the adaxial surface of leaves of four plant species Ttriticum aestivum (wheat), Zea mays (maize), Lupinus angustifolius (lupin), and Arabidopsis thaliana. In comparison with the application of surfactants there was less phytotoxicity on leaves of each species following treatment with NLCP. Following treatment of leaves with NLCP analysis of cuticular wax micromorphology revealed less wax solubilization in the monocot species. The results clearly show that there are advantages in the use of NLCP rather than surfactants for agrochemical delivery.
Publisher: Springer Science and Business Media LLC
Date: 03-1994
DOI: 10.1007/BF01666397
Publisher: MDPI AG
Date: 12-06-2021
Abstract: Pathogens and plants are in a constant battle with one another, the result of which is either the restriction of pathogen growth via constitutive or induced plant defense responses or the pathogen colonization of plant cells and tissues that cause disease. Elicitins are a group of highly conserved proteins produced by certain oomycete species, and their sterol binding ability is recognized as an important feature in sterol–auxotrophic oomycetes. Elicitins also orchestrate other aspects of the interactions of oomycetes with their plant hosts. The function of elicitins as avirulence or virulence factors is controversial and is dependent on the host species, and despite several decades of research, the function of these proteins remains elusive. We summarize here our current understanding of elicitins as either defense-promoting or defense-suppressing agents and propose that more recent approaches such as the use of ‘omics’ and gene editing can be used to unravel the role of elicitins in host–pathogen interactions. A better understanding of the role of elicitins is required and deciphering their role in host–pathogen interactions will expand the strategies that can be adopted to improve disease resistance and reduce crop losses.
Publisher: Springer Science and Business Media LLC
Date: 2003
Publisher: Elsevier BV
Date: 04-2016
Publisher: Springer Science and Business Media LLC
Date: 13-08-2017
Publisher: Scientific Societies
Date: 1989
Publisher: Springer Science and Business Media LLC
Date: 06-12-2006
DOI: 10.1007/S10142-006-0041-4
Abstract: Abscisic acid (ABA) has been implicated in determining the outcome of interactions between many plants and their pathogens. We had previously shown that increased concentrations of ABA within leaves of Arabidopsis induced susceptibility towards an avirulent strain of Pseudomonas syringae pathovar (pv.) tomato. We now show that ABA induces susceptibility via suppression of the accumulation of components crucial for a resistance response. Lignin and salicylic acid concentrations in leaves were increased during a resistant interaction but reduced when plants were treated with ABA. The reduction in lignin and salicylic acid production was independent of the development of the hypersensitive response (HR), indicating that, in this host-pathogen system, HR is not required for resistance. Genome-wide gene expression analysis using microarrays showed that treatment with ABA suppressed the expression of many defence-related genes, including those important for phenylpropanoid biosynthesis and those encoding resistance-related proteins. Together, these results show that resistance induction in Arabidopsis to an avirulent strain of P. syringae pv. tomato is regulated by ABA.
Publisher: Elsevier BV
Date: 05-1992
Publisher: Springer Science and Business Media LLC
Date: 18-10-2012
Publisher: Springer Science and Business Media LLC
Date: 04-11-2020
Publisher: Springer Science and Business Media LLC
Date: 13-05-2014
DOI: 10.1007/S00299-014-1624-5
Abstract: We report the uptake of MSNs into the roots and their movement to the aerial parts of four plant species and their quantification using fluorescence, TEM and proton-induced x - ray emission (micro - PIXE) elemental analysis. Monodispersed mesoporous silica nanoparticles (MSNs) of optimal size and configuration were synthesized for uptake by plant organs, tissues and cells. These monodispersed nanoparticles have a size of 20 nm with interconnected pores with an approximate diameter of 2.58 nm. There were no negative effects of MSNs on seed germination or when transported to different organs of the four plant species tested in this study. Most importantly, for the first time, a combination of confocal laser scanning microscopy, transmission electron microscopy and proton-induced X-ray emission (micro-PIXE) elemental analysis allowed the location and quantification MSNs in tissues and in cellular and sub-cellular locations. Our results show that MSNs penetrated into the roots via symplastic and apoplastic pathways and then via the conducting tissues of the xylem to the aerial parts of the plants including the stems and leaves. The translocation and widescale distribution of MSNs in plants will enable them to be used as a new delivery means for the transport of different sized biomolecules into plants.
Publisher: Informa UK Limited
Date: 13-12-2022
Publisher: Springer Science and Business Media LLC
Date: 13-01-2015
Publisher: CSIRO Publishing
Date: 2017
DOI: 10.1071/FP16266
Abstract: Resistance is rare against the oomycete plant pathogen Phytophthora cinnamomi Rands. Only a limited number of species have been recorded as field-resistant species in Australia. However, understanding the nature of resistance of those species when grown under controlled conditions is challenging because of their slow growth and the inherent difficulties of working with a root pathogen. We assessed the Australian native species, Lomandra longifolia Labill., as a resistant species by analysing in detail the response of roots to infection by P. cinnamomi in a series of comparative tests with Lupinus angustifolius L., a highly susceptible species. Following inoculation of L. longifolia roots, lesion length and colonisation percentage were significantly less than in roots of the susceptible species. Moreover, there was no statistical difference in root growth rate, whole-plant FW and leaf relative chlorophyll content between controls and inoculated L. longifolia. We then examined three key cellular responses that are related to resistance: the production of the reactive oxygen species, H2O2, callose formation and lignin deposition in L. longifolia roots following inoculation with P. cinnamomi. The upregulation of these resistance-related components in the early hours after inoculation suggested their involvement in resistance and that this is controlled by the coordinated response of multiple components. Resistance assessment and a detailed investigation of cellular resistance components along with gene expression analysis provides a platform for further understanding of the mechanisms of resistance against this generalist pathogen and presents opportunities for manipulating susceptible species for disease resistance.
Publisher: American Chemical Society (ACS)
Date: 29-12-2023
Publisher: Springer Science and Business Media LLC
Date: 20-06-2017
Publisher: Springer Science and Business Media LLC
Date: 2005
DOI: 10.1071/AP05074
Publisher: Oxford University Press (OUP)
Date: 22-07-2012
Abstract: The PHYTOCHROME AND FLOWERING TIME1 gene encoding the MEDIATOR25 (MED25) subunit of the eukaryotic Mediator complex is a positive regulator of jasmonate (JA)-responsive gene expression in Arabidopsis (Arabidopsis thaliana). Based on the function of the Mediator complex as a bridge between DNA-bound transcriptional activators and the RNA polymerase II complex, MED25 has been hypothesized to function in association with transcriptional regulators of the JA pathway. However, it is currently not known mechanistically how MED25 functions to regulate JA-responsive gene expression. In this study, we show that MED25 physically interacts with several key transcriptional regulators of the JA signaling pathway, including the APETALA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) transcription factors OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59 and ERF1 as well as the master regulator MYC2. Physical interaction detected between MED25 and four group IX AP2/ERF transcription factors was shown to require the activator interaction domain of MED25 as well as the recently discovered Conserved Motif IX-1/EDLL transcription activation motif of MED25-interacting AP2/ERFs. Using transcriptional activation experiments, we also show that OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59- and ERF1-dependent activation of PLANT DEFENSIN1.2 as well as MYC2-dependent activation of VEGETATIVE STORAGE PROTEIN1 requires a functional MED25. In addition, MED25 is required for MYC2-dependent repression of pathogen defense genes. These results suggest an important role for MED25 as an integrative hub within the Mediator complex during the regulation of JA-associated gene expression.
Publisher: Elsevier BV
Date: 2001
Publisher: Springer Science and Business Media LLC
Date: 15-09-2022
DOI: 10.1007/S11306-022-01933-4
Abstract: Chestnut rot caused by the fungus Gnomoniopsis smithogilvyi is a disease present in the world’s major chestnut growing regions. The disease is considered a significant threat to the global production of nuts from the sweet chestnut ( Castanea sativa ) . Conventional fungicides provide some control, but little is known about the potential of biological control agents (BCAs) as alternatives to manage the disease. Evaluate whether formulated BCAs and their secreted metabolites inhibit the in vitro growth of G. smithogilvyi. The antifungal potential of BCAs was assessed against the pathogen through an inverted plate assay for volatile compounds (VOCs), a diffusion assay for non-volatile compounds (nVOCs) and in dual culture. Methanolic extracts of nVOCs from the solid medium were further evaluated for their effect on conidia germination and were screened through an LC–MS-based approach for antifungal metabolites. Isolates of Trichoderma spp., derived from the BCAs, significantly suppressed the pathogen through the production of VOCs and nVOCs. The BCA from which Bacillus subtilis was isolated was more effective in growth inhibition through the production of nVOCs. The LC–MS based metabolomics on the nVOCs derived from the BCAs showed the presence of several antifungal compounds. The results show that G. smithogilvyi can be effectively controlled by the BCAs tested and that their use may provide a more ecological alternative for managing chestnut rot. The in vitro analysis should now be expanded to the field to assess the effectiveness of these alternatives for chestnut rot management.
Publisher: Springer Science and Business Media LLC
Date: 1999
Publisher: Elsevier BV
Date: 09-2011
DOI: 10.1016/J.SEMCDB.2011.07.012
Abstract: Since its original discovery in yeast, the Mediator complex has been identified in a wide range of organisms across the eukaryotic kingdom. Despite being experimentally purified from a number of fungal and metazoan organisms, it was not until 2007, thirteen years after its initial discovery, that the Mediator complex was successfully isolated from plants. With a number of papers now beginning to emerge on the plant Mediator complex, this review aims to provide an overview of the erse functions that have been identified for in idual plant Mediator subunits. In addition to demonstrating roles in plant development, flowering, hormone signaling and biotic and abiotic stress tolerance recent findings have revealed novel functions for plant Mediator subunits, including mRNA, miRNA and rRNA processing, as well as controlling DNA and protein stability. These erse activities have expanded the known functions of the Mediator complex and demonstrate a variety of new insights that have been gained from investigations into the plant Mediator complex. Future directions for research into this multi-functional protein complex will be discussed.
Publisher: Scientific Societies
Date: 1994
DOI: 10.1094/PHYTO-84-193
Publisher: Springer Science and Business Media LLC
Date: 25-02-2015
DOI: 10.1007/S00709-015-0777-6
Abstract: Electron microscopy techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) have been invaluable tools for the study of the micromorphology of plant cuticles. However, for electron microscopy, the preparation techniques required may invariably introduce artefacts in cuticle preservation. Further, there are a limited number of methods available for quantifying the image data obtained through electron microscopy. Therefore, in this study, optical microscopy techniques were coupled with staining procedures and, along with SEM were used to qualitatively and quantitatively assess the ultrastructure of plant leaf cuticles. Leaf cryosections of Triticum aestivum (wheat), Zea mays (maize), and Lupinus angustifolius (lupin) were stained with either fat-soluble azo stain Sudan IV or fluorescent, diarylmethane Auramine O and were observed under confocal laser scanning microscope (CLSM). For all the plant species tested, the cuticle on the leaf surfaces could be clearly resolved in many cases into cuticular proper (CP), external cuticular layer (ECL), and internal cuticular layer (ICL). Novel image data analysis procedures for quantifying the epicuticular wax micromorphology were developed, and epicuticular waxes of L. angustifolius were described here for the first time. Together, application of a multifaceted approach involving the use of a range of techniques to study the plant cuticle has led to a better understanding of cuticular structure and provides new insights into leaf surface architecture.
Publisher: Public Library of Science (PLoS)
Date: 30-04-2015
Publisher: Wiley
Date: 04-10-2018
DOI: 10.1111/PLB.12624
Abstract: The plant pathogen Phytophthora cinnamon the causal agent of disease in numerous species, is a major threat to natural vegetation and has economic impacts in agriculture. The pathogen principally invades the root system, which, in susceptible species, is rapidly colonised and functionally destroyed. Few species are resistant, however, where resistance is expressed the pathogen is restricted to small, localised lesions. The molecular mechanisms that underpin this response in resistant species are not well understood. Lomandra longifolia, an Australian native species, is highly resistant to P. cinnamomi. In an earlier study, we showed induction of resistance-related components such as callose, lignin and hydrogen peroxide (H
Publisher: American Chemical Society (ACS)
Date: 02-08-2016
Abstract: The addition of an adjuvant to a pesticide usually occurs in a mix-tank, before spray application to the crop. Their interaction is potentially crucial to overall efficacy but has received little attention from a physical-chemical perspective. Study was undertaken by laser diffraction, Raman spectroscopy, and small-angle X-ray scattering to resolve these physical processes. It was shown that migration of the pesticide into the adjuvant droplet occurred in all cases studied. The level of transfer was dependent upon adjuvant level, adjuvant solubility, and surfactant level. For suspension pesticides, dissolution of crystallites within the droplet occurred to a degree limited by solubility. The results directly demonstrate the transfer of the pesticide into the adjuvant carrier. This indicates that for emulsion-based pesticides, application to the target is likely as a homogeneously mixed droplet, whereas for suspension pesticides, solubility may limit transfer and dissolution, leading to heterogeneity in the applied particles.
Publisher: Springer Science and Business Media LLC
Date: 16-11-2018
Publisher: CSIRO Publishing
Date: 2013
DOI: 10.1071/FP13023
Abstract: In order to discover phytochemicals that are potentially bioactive against Phytophthora cinnamomi, (a soil-borne plant pathogen) a metabolite profiling protocol for investigation of metabolic changes in Lupinus angustifolius L. plant roots in response to pathogen challenge has been established. Analysis of the metabolic profiles from healthy and P. cinnamomi-inoculated root tissue with high resolution mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that although susceptible, L. angustifolius upregulated a defence associated genistein and 2′-hydroxygenistein-based isoflavonoid and a soyasapogenol saponin at 12 h post inoculation which increased in concentration at 72 h post inoculation. In contrast to the typical susceptible interaction, the application of a phosphorous-based treatment to L. angustifolius foliage 48 h before P. cinnamomi challenge negated the ability of the pathogen to colonise the root tissue and cause disease. Importantly, although the root profiles of water-treated and phosphite-treated plants post pathogen inoculation contained the same secondary metabolites, concentration variations were observed. Accumulation of secondary metabolites within the P. cinnamomi-inoculated plants confirms that pathogen ingress of the root interstitially occurs in phosphite-treated plants, confirming a direct mode of action against the pathogen upon breaching the root cells.
Publisher: Wiley
Date: 04-10-2021
Abstract: Plant cell culture systems have become an attractive and sustainable approach to produce high‐value and commercially significant metabolites under controlled conditions. Strategies involving elicitor supplementation into plant cell culture media are employed to mimic natural conditions for increasing the metabolite yield. Studies on nanoparticles (NPs) that have investigated elicitation of specialized metabolism have shown the potential of NPs to be a substitute for biotic elicitors such as phytohormones and microbial extracts. Customizable physicochemical characteristics allow the design of monodispersed‐, stimulus‐responsive‐, and hormone‐carrying‐NPs of precise geometries to enhance their elicitation capabilities based on target metabolite lant cell culture type. We contextualize advances in NP‐mediated elicitation, especially stimulation of specialized metabolic pathways, the underlying mechanisms, impacts on gene regulation, and NP‐associated cytotoxicity. The novelty of the concept lies in unleashing the potential of designer NPs to enhance yield, harness metabolites, and transform nanoelicitation from exploratory investigations to a commercially viable strategy.
Publisher: American Chemical Society (ACS)
Date: 05-2015
Abstract: The controlled release of salicylic acid (SA), a key phytohormone, was mediated by using a novel decanethiol gatekeeper system grafted onto mesoporous silica nanoparticles (MSNs). The decanethiol was conjugated only to the external surfaces of the MSNs through glutathione (GSH)-cleavable disulfide linkages and the introduction of a process to assemble gatekeepers only on the outer surface so that the mesopore area can be maintained for high cargo loading. Raman and nitrogen sorption isotherm analyses confirmed the successful linkage of decanethiol to the surface of MSNs. The in vitro release of SA from decanethiol gated MSNs indicated that the release rate of SA in an environment with a certain amount of GSH was significantly higher than that without GSH. More importantly, in planta experiments showed the release of SA from decanethiol gated MSNs by GSH induced sustained expression of the plant defense gene PR-1 up to 7 days after introduction, while free SA caused an early peak in PR-1 expression which steadily decreased after 3 days. This study demonstrates the redox-responsive release of a phytohormone in vitro and also indicates the potential use of MSNs in planta as a controlled agrochemical delivery system.
Publisher: Oxford University Press (OUP)
Date: 30-07-2008
Publisher: Elsevier BV
Date: 06-2016
DOI: 10.1016/J.CHEMOSPHERE.2016.02.096
Abstract: The application of mesoporous silica nanoparticles (MSNs) as a smart delivery system to agricultural crops is gaining attention but the release of nanoparticles into the environment may pose a potential threat to biological systems. We investigated the effects of MSNs on the growth and development of wheat and lupin plants grown under controlled conditions. We report a dramatic increase in the growth of wheat and lupin plants exposed to MSNs. We also found that, in leaves, MSNs localised to chloroplasts and that photosynthetic activity was significantly increased. In addition, absorption and cellular distribution of MSNs by the two plant species following root uptake were observed using scanning electron microscopy equipped with energy dispersive spectroscopy (SEM-EDS). Following uptake of MSNs at 500 and 1000 mg L(-1), there was enhancement of seed germination, increased plant biomass, total protein and chlorophyll content. Treatment of both species with MSNs at the highest concentration (2000 mg L(-1)) did not result in oxidative stress or cell membrane damage. These findings show that MSNs can be used as novel delivery systems in plants and that over the range of concentrations tested, MSNs do not have any negative impacts on plant growth or development.
Publisher: American Society for Microbiology
Date: 05-05-2020
DOI: 10.1128/AEM.00375-20
Abstract: (+)-Pinoresinol is an important plant defense compound, a major food lignan for humans and some other animals, and the model compound used to study degradation of the β-β′ linkages in lignin. We report a gene cluster, in one strain each of Pseudomonas and Burkholderia , that is involved in the oxidative catabolism of (+)-pinoresinol. The flavoprotein component of the α-hydroxylase which heads the pathway belongs to the 4-phenol oxidizing (4PO) subgroup of the vanillyl alcohol oxidase/ p -cresol methyl hydroxylase (VAO/PCMH) enzyme family but constitutes a novel combination of cofactor and electron acceptor properties for the family. It is translationally coupled with a cytochrome gene whose product is also required for activity. The work casts new light on the biology of (+)-pinoresinol and its transformation to other bioactive molecules. Potential applications of the findings include new options for deconstructing lignin into useful chemicals and the generation of new phytoestrogenic enterolactones from lignans.
Publisher: Springer Science and Business Media LLC
Date: 2003
DOI: 10.1071/AP03048
Publisher: Wiley
Date: 10-2002
Publisher: Scientific Societies
Date: 1989
DOI: 10.1094/PHYTO-79-417
Publisher: Elsevier BV
Date: 02-1993
Publisher: Springer Science and Business Media LLC
Date: 2003
DOI: 10.1071/AP03041
Publisher: Springer Science and Business Media LLC
Date: 17-03-2016
Publisher: Informa UK Limited
Date: 09-1999
Publisher: Wiley
Date: 05-1986
Publisher: Hindawi Limited
Date: 2015
DOI: 10.1155/2015/982412
Abstract: Catharanthus roseus (L.) known as Madagascar periwinkle (MP) is a legendary medicinal plant mostly because of possessing two invaluable antitumor terpenoid indole alkaloids (TIAs), vincristine and vinblastine. The plant has also high aesthetic value as an evergreen ornamental that yields prolific blooms of splendid colors. The plant possesses yet another unique characteristic as an amiable experimental host for the maintenance of the smallest bacteria found on earth, the phytoplasmas and spiroplasmas, and serves as a model for their study. Botanical information with respect to synonyms, vernacular names, cultivars, floral morphology, and reproduction adds to understanding of the plant while the geography and ecology of periwinkle illustrate the organism’s ubiquity. Good agronomic practices ensure generous propagation of healthy plants that serve as a source of bioactive compounds and multitudinous horticultural applications. The correlation between genetic ersity, variants, and TIA production exists. MP is afflicted with a whole range of diseases that have to be properly managed. The ethnobotanical significance of MP is exemplified by its international usage as a traditional remedy for abundant ailments and not only for cancer. TIAs are present only in micro quantities in the plant and are highly poisonous per se rendering a challenge for researchers to increase yield and reduce toxicity.
Publisher: Wiley
Date: 11-12-2014
DOI: 10.1111/MPP.12190
Publisher: Wiley
Date: 12-1985
Publisher: Springer Science and Business Media LLC
Date: 03-02-2014
Publisher: CSIRO Publishing
Date: 2007
DOI: 10.1071/FP06316
Abstract: Natriuretic peptides (NP) were first identified in animals where they play a role in the regulation of salt and water balance. This regulation is partly mediated by intracellular changes in cyclic GMP (cGMP). NP immunoanalogues occur in many plants and have been isolated, with two NP encoding genes characterised in Arabidopsis thaliana L. (AtPNP-A and AtPNP-B). Part of AtPNP-A contains the region with homology to human atrial (A)NP. We report here on the effects of recombinant AtPNP-A and smaller synthetic peptides within the ANP-homologous region with a view to identifying the biologically active domain of the molecule. Furthermore, we investigated interactions between AtPNP-A and the hormone, abscisic acid (ABA). ABA does not significantly affect Arabidopsis mesophyll protoplast volume regulation, whereas AtPNP-A and synthetic peptides promote water uptake into the protoplasts causing swelling. This effect is promoted by the membrane permeable cGMP analogue, 8-Br-cGMP, and inhibited by guanylate cyclase inhibitors indicating that increases in cGMP are an essential component of the plant natriuretic peptides (PNP) signalling cascade. ABA does not induce cGMP transients and does not affect AtPNP-A dependent cGMP increases, hence the two regulators differ in their second messenger signatures. Interestingly, AtPNP-A significantly delays and reduces the extent of ABA stimulated stomatal closure that is also based on cell volume regulation. We conclude that a complex interplay between observed PNP effects (stomatal opening and protoplast swelling) and ABA is likely to be cell type specific.
Publisher: Frontiers Media SA
Date: 28-03-2018
Publisher: Scientific Societies
Date: 1994
Publisher: Wiley
Date: 05-12-2014
DOI: 10.1111/PLB.12286
Abstract: Exposure of plants to UV-C irradiation induces gene expression and cellular responses that are commonly associated with wounding and pathogen defence, and in some cases can lead to increased resistance against pathogen infection. We examined, at a physiological, molecular and biochemical level, the effects of and responses to, sub-lethal UV-C exposure on Arabidopsis plants when irradiated with increasing dosages of UV-C radiation. Following UV-C exposure plants had reduced leaf areas over time, with the severity of reduction increasing with dosage. Severe morphological changes that included leaf glazing, bronzing and curling were found to occur in plants treated with the 1000 J·m(-2) dosage. Extensive damage to the mesophyll was observed, and cell death occurred in both a dosage- and time-dependent manner. Analysis of H₂O₂ activity and the pathogen defence marker genes PR1 and PDF1.2 demonstrated induction of these defence-related responses at each UV-C dosage tested. Interestingly, in response to UV-C irradiation the production of callose (β-1,3-glucan) was identified at all dosages examined. Together, these results show plant responses to UV-C irradiation at much lower doses than have previously been reported, and that there is potential for the use of UV-C as an inducer of plant defence.
Publisher: MDPI AG
Date: 16-11-2021
Abstract: Plant priming is an induced physiological state where plants are protected from biotic and abiotic stresses. Whether seaweed extracts promote priming is largely unknown as is the mechanism by which priming may occur. In this study, we examined the effect of a seaweed extract (SWE) on two distinct stages of plant priming (priming phase and post-challenge primed state) by characterising (i) plant gene expression responses using qRT-PCR and (ii) signal transduction responses by evaluating reactive oxygen species (ROS) production. The SWE is made from the brown algae Ascophyllum nodosum and Durvillaea potatorum. The priming phase was examined using both Arabidopsis thaliana and Solanum lycopersicum. At this stage, the SWE up-regulated key priming-related genes, such as those related to systemic acquired resistance (SAR) and activated the production of ROS. These responses were found to be temporal (lasting 3 days). The post-challenge primed state was examined using A. thaliana challenged with a root pathogen. Similarly, defence response-related genes, such as PR1 and NPR1, were up-regulated and ROS production was activated (lasting 5 days). This study found that SWE induces plant priming-like responses by (i) up-regulating genes associated with plant defence responses and (ii) increasing production of ROS associated with signalling responses.
Publisher: Springer Science and Business Media LLC
Date: 14-11-2022
DOI: 10.1038/S41598-022-20772-W
Abstract: The ongoing and unrestrained application of nitrogen fertilizer to agricultural lands has been directly linked to climate change and reductions in bio ersity. The agricultural sector needs a technological upgrade to adopt sustainable methods for maintaining high yield. We report synthesis of zinc and magnesium doped and undoped hydroxyapatite nanoparticles, and their urea nanohybrids, to sustainably deliver nitrogen to wheat. The urea nanohybrids loaded with up to 42% nitrogen were used as a new source of nitrogen and compared with a conventional urea-based fertilizer for efficient and sufficient nitrogen delivery to pot-grown wheat. Doping with zinc and magnesium manipulated the hydroxyapatite crystallinity for smaller size and higher nitrogen loading capacity. Interestingly, 50% and 25% doses of urea nanohybrids significantly boosted the wheat growth and yield compared with 100% doses of urea fertilizer. In addition, the nutritional elements uptake and grain protein and phospholipid levels were significantly enhanced in wheat treated with nanohybrids. These results demonstrate the potential of the multi-nutrient complexes, the zinc and magnesium doped and undoped hydroxyapatite-urea nanoparticles, as nitrogen delivery agents that reduce nitrogen inputs by at least 50% while maintaining wheat plant growth and nitrogen uptake to the same level as full-dose urea treatments.
Publisher: Springer Science and Business Media LLC
Date: 2003
DOI: 10.1071/AP02064
Publisher: Elsevier BV
Date: 12-2013
Publisher: CSIRO Publishing
Date: 1995
DOI: 10.1071/AR9951311
Abstract: Phytophthora spp. are one of Australia's most serious plant pathogens, causing well in excess of $200 million in lost production annually across all of our plant-based industries. Of the more than 50 species of Phytophthora recorded worldwide, over 20 are present in Australia. All species, along with their hosts, distribution and current control measures have been tabulated. Use of molecular markers to analyse population genetic structure has shown that most of these pathogens have been introduced from overseas, and become widely distributed in Australia within relatively short periods of time. The review highlights the general biology of Phytophthora spp. and their impact on agricultural production in Australia, with particular emphasis on the genetics of host athogen interactions, population genetic structure and reproduction of Phytophthora populations in Australia. The review highlights the role molecular technologies may play in developing an understanding of host-pathogen recognition and pathogenesis in Phytophthora. Three case histories, involving P. sojae, P. medicaginis and P. cinnamomi, have been developed to provide insights into current levels of understanding, and to identify gaps in our knowledge. Current control measures are summarized and possible new avenues of control discussed.
Publisher: CSIRO Publishing
Date: 2017
DOI: 10.1071/PC16032
Abstract: The introduction and subsequent impact of Phytophthora cinnamomi within native vegetation is one of the major conservation issues for bio ersity in Australia. Recently, many new Phytophthora species have been described from Australia’s native ecosystems however, their distribution, origin, and potential impact remain unknown. Historical bias in Phytophthora detection has been towards sites showing symptoms of disease, and traditional isolation methods show variable effectiveness of detecting different Phytophthora species. However, we now have at our disposal new techniques based on the s ling of environmental DNA and metabarcoding through the use of high-throughput sequencing. Here, we report on the ersity and distribution of Phytophthora in Australia using metabarcoding of 640 soil s les and we compare the ersity detected using this technique with that available in curated databases. Phytophthora was detected in 65% of sites, and phylogenetic analysis revealed 68 distinct Phytophthora phylotypes. Of these, 21 were identified as potentially unique taxa and 25 were new detections in natural areas and/or new introductions to Australia. There are 66 Phytophthora taxa listed in Australian databases, 43 of which were also detected in this metabarcoding study. This study revealed high Phytophthora richness within native vegetation and the additional records provide a valuable baseline resource for future studies. Many of the Phytophthora species now uncovered in Australia’s native ecosystems are newly described and until more is known we need to be cautious with regard to the spread and conservation management of these new species in Australia’s unique ecosystems.
Publisher: American Chemical Society (ACS)
Date: 05-2023
Publisher: Springer Science and Business Media LLC
Date: 18-04-2015
DOI: 10.1007/S00438-015-1046-2
Abstract: Invasive phytoplasmas wreak havoc on coconut palms worldwide, leading to high loss of income, food insecurity and extreme poverty of farmers in producing countries. Phytoplasmas as strictly biotrophic insect-transmitted bacterial pathogens instigate distinct changes in developmental processes and defence responses of the infected plants and manipulate plants to their own advantage however, little is known about the cellular and molecular mechanisms underlying host-phytoplasma interactions. Further, phytoplasma-mediated transcriptional alterations in coconut palm genes have not yet been identified. This study evaluated the whole transcriptome profiles of naturally infected leaves of Cocos nucifera ecotype Malayan Red Dwarf in response to yellow decline phytoplasma from group 16SrXIV, using RNA-Seq technique. Transcriptomics-based analysis reported here identified genes involved in coconut innate immunity. The number of down-regulated genes in response to phytoplasma infection exceeded the number of genes up-regulated. Of the 39,873 differentially expressed unigenes, 21,860 unigenes were suppressed and 18,013 were induced following infection. Comparative analysis revealed that genes associated with defence signalling against biotic stimuli were significantly overexpressed in phytoplasma-infected leaves versus healthy coconut leaves. Genes involving cell rescue and defence, cellular transport, oxidative stress, hormone stimulus and metabolism, photosynthesis reduction, transcription and biosynthesis of secondary metabolites were differentially represented. Our transcriptome analysis unveiled a core set of genes associated with defence of coconut in response to phytoplasma attack, although several novel defence response candidate genes with unknown function have also been identified. This study constitutes valuable sequence resource for uncovering the resistance genes and/or susceptibility genes which can be used as genetic tools in disease resistance breeding.
Publisher: Informa UK Limited
Date: 11-07-2023
DOI: 10.1080/07388551.2022.2090315
Abstract: Graphene has triggered enormous interest in, and exploration of, its applications in erse areas of science and technology due to its unique properties. While graphene has displayed great potential as a nano-delivery system for drugs and biomolecules in biomedicine, its application as a nanocarrier in agriculture has only begun to be explored. Conventional fertilizers and agricultural delivery systems have a number of disadvantages, such as: fast release of the active ingredient, low delivery efficiency, rapid degradation and low stability that often leads to their over-application and consequent environmental problems. Advanced nano fertilizers with high carrier efficiency and slow and controlled release are now considered the gold standard for promoting agricultural sustainability while protecting the environment. Graphene's attractive properties include large surface area, chemical stability, mechanical stability, tunable surface chemistry and low toxicity making it a promising material on which to base agricultural delivery systems. Recent research has demonstrated considerable success in the use of graphene for agricultural applications, including its utilization as a delivery vehicle for plant nutrients and crop protection agents, as well as in post-harvest management of crops. This review, therefore, presents a comprehensive overview of the current status of graphene-based nanocarriers in agriculture. Additionally, the review outlines the surface functionalization methods used for effective molecular delivery, various strategies for nano-vehicle design and the underlying features necessary for a graphene-based agro-delivery system. Finally, the review discusses directions for further research in optimization of graphene-based nanocarriers.
Publisher: Wiley
Date: 06-07-2009
DOI: 10.1111/J.1439-0434.2008.01503.X
Abstract: Analysis of the interaction of pathogens with plant roots is often complicated by the growth of plants in a soil substrate. A soil‐free plant growth system (SPS) was developed that removes the need for a substrate while supporting the growth of seedlings in a nutrient rich, oxygenated environment. The model legume Lupinus angustifolius was used to compare the growth of seedlings within soil and the SPS. Seedlings grown under both conditions were similar in morphology, anatomy and health (measured by leaf chlorophyll abundance) and importantly there was little difference in root growth and development although straighter and fuller root systems were achieved in the SPS. The ease of access to the root system proved efficient for the analysis of root and pathogen interactions with no interference from soil or adhering particulate matter. Following inoculation of L. angustifolius roots with Phytophthora cinnamomi the host athogen interaction was easily observed and tissues s led undamaged.
Publisher: Wiley
Date: 04-04-2016
DOI: 10.1111/MPP.12361
Publisher: Springer Science and Business Media LLC
Date: 1985
DOI: 10.1071/APP9850059
Publisher: Springer Science and Business Media LLC
Date: 1999
DOI: 10.1071/AP99005
Publisher: CSIRO Publishing
Date: 2003
DOI: 10.1071/FP02231
Abstract: The phytohormone abscisic acid (ABA) plays a major role in the regulation of many physiological stresses although its role in pathogen-induced stress remains poorly understood. We examined the influence of ABA on interactions of Arabidopsis thaliana (L.) Heynh. (Arabidopsis) with a bacterial pathogen, Pseudomonas syringae pv. tomato and an Oomycete, Peronospora parasitica. Both addition of 100 μM ABA to plants and drought stress stimulated increased susceptibility of Arabidopsis to an avirulent isolate of P. syringae pv. tomato. In contrast, an ABA-deficient mutant of Arabidopsis, aba1-1, displayed reduced susceptibility to virulent isolates of P. parasitica. An ABA-insensitive mutant, abi1-1, that is impaired in ABA signal transduction did not alter in susceptibility to either P. syringae pv. tomato or P. parasitica. These results demonstrate that the concentration of endogenous ABA at the time of pathogen challenge is important for the development of susceptibility in Arabidopsis.
Publisher: American Chemical Society (ACS)
Date: 20-12-0020
Publisher: Elsevier BV
Date: 09-2019
Publisher: Springer Science and Business Media LLC
Date: 21-11-2018
Publisher: American Chemical Society (ACS)
Date: 24-04-2023
Publisher: Elsevier BV
Date: 12-2018
DOI: 10.1016/J.CHEMOSPHERE.2018.08.057
Abstract: To investigate the bacterial mechanisms of polycyclic aromatic hydrocarbons (PAHs) degradation in an aged-contaminated agricultural soil planted with tall fescue (Festuca arundinacea), a rhizo-box experiment was carried out for 60 d. Shifts in bacterial community structure in the soils during the experiment were performed using denaturing gradient gel electrophoresis. The abundance and activity of total bacteria and PAH-degraders were measured by quantification of 16S rDNA, PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes and their transcripts, respectively. The residual PAH concentrations were monitored using high-performance liquid chromatography analysis. Results showed that the removal percentage of total PAHs in rhizosphere soil was 11% higher than that in unplanted soil. Soil bacteria were dominated by Alphaproteobacteria (48.4%) and Gammaproteobacteria (25.8%). Tall fescue positively affected the abundance and activity of total bacteria in the soil, and stimulated RHDα gram-negative (GN) gene expression while inhibiting RHDα gram-positive gene expression. PAH dissipation in rhizosphere soil could be ascribed to modifications in the bacterial community structure, increase in the abundance of PAH-degraders, and enhancement of the RHDα GN gene expression during the incubation.
Publisher: Informa UK Limited
Date: 02-03-2021
DOI: 10.1080/10495398.2021.1885428
Abstract: Efficient isolation of genetically modified cells that are phenotypically indistinguishable from the unmodified cells remains a major technical barrier for the broader utilization of CRISPR/Cas9. Here, we report a novel enrichment approach to select the genome engineered cells by co-targeting a genomically integrated GFP gene along with the endogenous gene of interest (GOI). Using this co-targeting approach, multiple genomic loci were successfully targeted in chicken (DF1) and quail (CEC-32) fibroblast cell lines by transient transfection of Cas9 and guide RNAs (gRNAs). Clonal isolation of co-targeted DF1 cells showed 75% of cell clones had deletion of GFP and biallelic deletion of the GOI. To assess the utility of this approach to generate genome modified animals, we tested it on chicken primordial germ cells (PGCs) expressing GFP by co-targeting with gRNAs against GFP and endogenous ovomucoid (OVM) gene. PGCs enriched for loss of GFP and confirmed for OVM deletion, derived by co-targeting, were injected into Hamburger and Hamilton stage 14-15 chicken embryos, and their ability to migrate to the genital ridge was confirmed. This simple, efficient enrichment approach could easily be applied to the creation of knock-out or edited cell lines or animals.
Publisher: Elsevier BV
Date: 12-1989
Publisher: Wiley
Date: 28-02-2010
Publisher: Elsevier BV
Date: 08-2001
Publisher: Elsevier BV
Date: 10-2017
Publisher: Elsevier BV
Date: 09-2007
DOI: 10.1016/J.JNEUMETH.2007.06.009
Abstract: Cell culture analyses of growth, morphology and apoptosis commonly require counting of different cell types stained with antibodies to discriminate between them. Previously, we reported the use of l-Leucine methyl ester (l-LME) to prepare purified cultures of type 1 astrocytes with minimal microglia, and staining by GFAP and CD antibodies, respectively. Here, we demonstrate a novel use of acridine orange (AO) for rapid discrimination between these cell types using fluorescence microscopy. AO accumulates in the lysosomes and also binds strongly to nuclear DNA and cytoplasmic/nucleolar RNA. Microglia may contain abundant lysosomes due to known roles in homeostasis and immune response. AO staining of lysosomes was tested at a range of concentrations, and 2.5 microg/mL was most suitable. In agreement with previous reports, microglia treated with AO showed very intense yellow, orange or red granular cytoplasmic staining of lysosomes. Microglia contain a substantially higher number of lysosomes than astrocytes, which have a variable amount. We measured the microglia population at 5.14+/-0.50% in mixed cultures. Thus, these results show AO is a novel discriminatory marker, as microglia were easily observed and counted in clumps on top of the monolayer of astrocytes, providing a rapid alternative to time-consuming and costly antibody-based assays.
Publisher: CSIRO Publishing
Date: 2001
DOI: 10.1071/BT00065
Abstract: Over the past 30 years, heathland and open forest communities in south-eastern Australia dominated by Xanthorrhoea australis R.Br. have been severely affected by disease caused by Phytophthora cinnamomi Rands. The disease has caused a sharp decline in numbers of in iduals within populations of X. australis however, the etiology of the disease is unclear. The characteristics and disease symptoms induced by P. cinnamomi were analysed within nine mature X. australis plants that had been removed from the field. Seven plants showed typical disease symptoms that ranged from chlorotic leaves through to plant death. Plants showing disease symptoms had different numbers of infected roots, ranging from 0% in one dead plant, 40% infected roots in a plant showing yellowing of leaf tips and 67 and 86%, respectively, in two plants with severe chlorosis. There was variation within the roots, with some infected close to the stem while others were infected at more distal regions. Within stems of all plants, P. cinnamomi was difficult to isolate but was found in the desmium and stem apex and was associated with massive lesions within the central area of the stem. The symptoms of disease in X. australis are caused by a combination of damage to tissues of the roots and stem that may lead to a reduction in water and mineral transport throughout the plant.
Publisher: Elsevier BV
Date: 09-1999
Publisher: Informa UK Limited
Date: 22-01-2017
Publisher: CSIRO Publishing
Date: 2000
DOI: 10.1071/BT98067
Abstract: The Eastern Otway Ranges, Victoria is an area recognised for its significant flora and fauna communities. An area of about 7000 ha, the Anglesea heathlands has been listed on the register of the National Estate. Although symptoms of the plant pathogen Phytophthora cinnamomi have been recorded in the area since the 1970s, the extent of infestation and its effects have not been documented. The aims of this study were to assess the distribution of infestation, identify the relationship of plant infection to site variables and to identify evidence of spread of the pathogen. The presence of P. cinnamomi was assessed at 50 study sites by a visual evaluation of the vegetation, using species such as Xanthorrhoea australis (Austral grass tree) and Isopogon ceratophyllus (horny cone bush) as indicator species. Soil and plant tissues were assayed for the presence of the pathogen by cotyledon baiting and immunoassay methods. Phytophthora cinnamomi was isolated at 76% of the study sites from the floristic communities identified: heathy open forest heathy woodland I and II Bald Hills heathland riparian open forest. There were significantly more diseased sites at lower altitudes. The pathogen is widely spread and causing disease throughout the area, but at present often only in small patches. There is an urgent need for disease management to protect the significant communities. These findings suggest that management actions such as mapping current infestations, closing tracks and implementing vehicle wash-down points are required.
Publisher: CSIRO Publishing
Date: 2023
DOI: 10.1071/FP22274
Publisher: Frontiers Media SA
Date: 2017
Publisher: Springer Science and Business Media LLC
Date: 02-08-2022
DOI: 10.1007/S13313-022-00879-4
Abstract: Chestnut rot caused by Gnomoniopsis smithogilvyi is considered a severe threat to the production of chestnuts from European chestnut ( Castanea sativa Mill.) in Australia and overseas. Currently, most of the control strategies are applied post-harvest and little is known about the use of fungicides to reduce nut infection in orchards early in the season. This research evaluated the effectiveness of various fungicides against the pathogen in vitro and selected the most effective products for field trials. In vitro experiments showed that pyraclostrobin and difenoconazole-based fungicides effectively inhibited conidial germination and mycelial growth, respectively. The field trial showed that both active ingredients combined were more effective than single applications in suppressing the level of nut infection caused by G. smithogilvyi . Based on our results, using the selected fungicides can be an additional tool for growers to complement their current practices in the control of chestnut rot. To the best of our knowledge, this is the first study focused on the effectiveness of chemical treatments against G. smithogilvyi .
Publisher: American Chemical Society (ACS)
Date: 15-04-2022
Publisher: Wiley
Date: 12-1993
Publisher: Springer Science and Business Media LLC
Date: 2007
Publisher: Springer Science and Business Media LLC
Date: 05-05-2013
Publisher: Informa UK Limited
Date: 11-07-2023
DOI: 10.1080/07388551.2022.2092717
Abstract: Addressing nutritional deficiencies in food crops through biofortification is a sustainable approach to tackling malnutrition. Biofortification is continuously being attempted through conventional breeding as well as through various plant biotechnological interventions, ranging from molecular breeding to genetic engineering and genome editing for enriching crops with various health-promoting metabolites. Genetic engineering is used for the rational incorporation of desired nutritional traits in food crops and predominantly operates through nuclear and chloroplast genome engineering. In the recent past, chloroplast engineering has been deployed as a strategic tool to develop model plants with enhanced nutritional traits due to the various advantages it offers over nuclear genome engineering. However, this approach needs to be extended for the nutritional enhancement of major food crops. Further, this platform could be combined with strategies, such as synthetic biology, chloroplast editing, nanoparticle-mediated rapid chloroplast transformation, and horizontal gene transfer through grafting for targeting endogenous metabolic pathways for overproducing native nutraceuticals, production of biopharmaceuticals, and biosynthesis of designer nutritional compounds. This review focuses on exploring various features of chloroplast genome engineering for nutritional enhancement of food crops by enhancing the levels of existing metabolites, restoring the metabolites lost during crop domestication, and introducing novel metabolites and phytonutrients needed for a healthy daily diet.
Publisher: MDPI AG
Date: 10-02-2022
DOI: 10.3390/MPS5010018
Abstract: Exogenous RNA polymerase III (pol III) promoters are commonly used to express short hairpin RNA (shRNA). Previous studies have indicated that expression of shRNAs using standard pol III promoters can cause toxicity in vivo due to saturation of the native miRNA pathway. A potential way of mitigating shRNA-associated toxicity is by utilising native miRNA processing enzymes to attain tolerable shRNA expression levels. Here, we examined parallel processing of exogenous shRNAs by harnessing the natural miRNA processing enzymes and positioning a shRNA adjacent to microRNA107 (miR107), located in the intron 5 of the Pantothenate Kinase 1 (PANK1) gene. We developed a vector encoding the PANK1 intron containing miR107 and examined the expression of a single shRNA or multiple shRNAs. Using qRT-PCR analysis and luciferase assay-based knockdown assay, we confirmed that miR30-structured shRNAs have resulted in the highest expression and subsequent transcript knockdown. Next, we injected Hamburger and Hamilton stage 14–15 chicken embryos with a vector encoding multiple shRNAs and confirmed that the parallel processing was not toxic. Taken together, this data provides a novel strategy to harness the native miRNA processing pathways for shRNA expression. This enables new opportunities for RNAi based applications in animal species such as chickens.
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/FP10073
Abstract: Plants constantly come into contact with a erse range of microorganisms that are potential pathogens, and they have evolved multi-faceted physical and chemical strategies to inhibit pathogen ingress and establishment of disease. Microbes, however, have developed their own strategies to counteract plant defence responses. Recent research on plant–microbe interactions has revealed that an important part of the infection strategies of a erse range of plant pathogens, including bacteria, fungi and oomycetes, is the production of effector proteins that are secreted by the pathogen and that promote successful infection by manipulating plant structure and metabolism, including interference in plant defence mechanisms. Pathogen effector proteins may function either in the extracellular spaces within plant tissues or within the plant cell cytoplasm. Extracellular effectors include cell wall degrading enzymes and inhibitors of plant enzymes that attack invading pathogens. Intracellular effectors move into the plant cell cytoplasm by as yet unknown mechanisms where, in incompatible interactions, they may be recognised by plant resistance proteins but where, in compatible interactions, they may suppress the plant’s immune response. This article presents a brief overview of our current understanding of the nature and function of effectors produced by oomycete plant pathogens.
Publisher: Elsevier BV
Date: 08-1982
Publisher: Elsevier BV
Date: 08-2019
Publisher: Elsevier BV
Date: 06-2017
DOI: 10.1016/J.ECOENV.2017.02.044
Abstract: The aims of this study were to evaluate the bioavailability of polycyclic aromatic hydrocarbons (PAHs) in industrial and agricultural soils using chemical methods and a bioassay, and to study the relationships between the methods. This was conducted by comparing the quantities of PAHs extracted from two manufactured gas plant (MGP) soils and an agricultural soil with low level contamination by solid-phase micro-extraction (SPME) and Tenax-TA extraction with the quantities taken up by the earthworm (Eisenia fetida). In addition, a biodegradation experiment was conducted on one MGP soil (MGP-A) to clarify the relationship between PAH removal by biodegradation and the variation in PAH concentrations in soil pore water. Results demonstrated that the earthworm bioassay could not be used to examine PAH bioavailability in the tested MGP soils which was the case even in the diluted MGP-A soils after biodegradation. However, the bioassay was successfully applied to the agricultural soil. These results suggest that earthworms can only be used for bioassays in soils with low toxicity. In general, rapidly desorbing concentrations extracted by Tenax-TA could predict PAH concentrations accumulated in earthworms (R
Publisher: Informa UK Limited
Date: 31-01-2018
Publisher: American Chemical Society (ACS)
Date: 02-10-2014
DOI: 10.1021/JF502709E
Abstract: Plants are an important source for medicinal compounds. Chemical screening and selection is critical for identification of compounds of interest. Ocimum basilicum (Basil) is a rich source of polyphenolics and exhibits high ersity, therefore bioprospecting of a suitable cultivar is a necessity. This study reports on the development of a true to type novel "in vitro system" and its comparison with a conventional system for screening and selection of cultivars for high total phenolics, in idual polyphenolics, and antioxidant content. We have shown for the first time using online acidic potassium permanganate chemiluminescence that extracts from Ocimum basilicum showed antioxidant potential. The current study identified the cultivar specific composition of polyphenolics and their antioxidant properties. Further, a distinct relationship between plant morphotype and polyphenolic content was also found. Of the 15 cultivars examined, "Holy Green", "Red Rubin", and "Basil Genovese" were identified as high polyphenolic producing cultivars while "Subja" was determined to be a low producer. The "in vitro system" enabled differentiation of the cultivars in their morphology, polyphenolic content, and antioxidant activity and is a cheap and efficient method for bioprospecting studies.
Publisher: CSIRO Publishing
Date: 2007
DOI: 10.1071/EA05162
Abstract: Annual ryegrass toxicity (ARGT) is responsible for significant stock losses in South Australia and Western Australia. The toxicity is caused by corynetoxins produced by the bacterium Rathayibacter toxicus (with the possible involvement of a bacteriophage), which infects annual ryegrass (Lolium rigidum). Polymerase chain reaction (PCR)-based assays, compatible with an existing enzyme-linked immunosorbent assay for the corynetoxins, have been developed and used to screen L. rigidum for both the presence of R. toxicus and for the bacteriophage isolate NCPPB 3778. The results from analysing bacterially infected galls from toxic grain screenings showed a positive correlation between the presence of the bacterium and corynetoxins but not with the bacteriophage. Analysis of pasture-derived s les of annual ryegrass showed about a 50% correlation of corynetoxins with bacterial presence and about a 5% correlation of phage with the presence of the bacterium. These observations support the potential application of the PCR-based assays in providing a useful, complementary tool in the assessment of the likelihood of pasture and feed to cause ARGT and to enable a better understanding of the complex aetiology of ARGT.
Publisher: Springer Science and Business Media LLC
Date: 02-02-2016
Publisher: Elsevier BV
Date: 05-2004
Publisher: Springer Science and Business Media LLC
Date: 2009
DOI: 10.1071/AP08092
Publisher: Springer Science and Business Media LLC
Date: 2009
DOI: 10.1071/AP08093
Publisher: American Chemical Society (ACS)
Date: 05-12-2018
Publisher: Elsevier BV
Date: 03-1989
Start Date: 2011
End Date: 2013
Funder: Australian Research Council
View Funded ActivityStart Date: 2010
End Date: 2010
Funder: Australian Research Council
View Funded ActivityStart Date: 2006
End Date: 2006
Funder: Australian Research Council
View Funded ActivityStart Date: 2006
End Date: 2008
Funder: Australian Research Council
View Funded ActivityStart Date: 2013
End Date: 2016
Funder: Australian Research Council
View Funded ActivityStart Date: 2002
End Date: 2004
Funder: Australian Research Council
View Funded ActivityStart Date: 2009
End Date: 2012
Funder: Australian Research Council
View Funded ActivityStart Date: 2005
End Date: 2007
Funder: Australian Research Council
View Funded ActivityStart Date: 2021
End Date: 2026
Funder: Australian Research Council
View Funded ActivityStart Date: 2022
End Date: 2025
Funder: Australian Research Council
View Funded Activity