ORCID Profile
0000-0001-6631-246X
Current Organisations
University of Science and Technology of China
,
Murdoch University
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Publisher: Frontiers Media SA
Date: 25-02-2021
Abstract: Cancer is a life-threatening concern worldwide. Sensitive and early-stage diagnostics of different cancer types can make it possible for patients to get through the best available treatment options to combat this menace. Among several new detection methods, aptamer-based biosensors (aptasensors) have recently shown promising results in terms of sensitivity, identification, or detection of either cancerous cells or the associated biomarkers. In this mini-review, we have summarized the most recent (2016–2020) developments in different approaches belonging to optical aptasensor technologies being widely employed for their simple operation, sensitivity, and early cancer diagnostics. Finally, we shed some light on limitations, advantages, and current challenges of aptasensors in clinical diagnostics, and we elaborated on some future perspectives.
Publisher: Elsevier BV
Date: 11-2022
DOI: 10.1016/J.BIOS.2022.114509
Abstract: Despite their high analytical performance, conventional analytical biosensor devices are usually difficult to handle, time-consuming, bulky and expensive. As a result, their applications remain restricted to resource-limited environments. In particular, the transportation of conventional analytical equipment is challenging for the proper in-situ point of need (PON) oint of care (POC) detection of biomolecules. In this context, smartphones, the most widely utilized cutting-edge mobile gadgets, continue to be a favored option because of their ease of portability and revolutionary sensing capabilities. On the other hand, aptamers are molecular recognition units consisting of nucleic acids with highly sensitive and selective recognition capabilities towards their respective targets. The coupling of smartphones with aptamers have led the development of advanced, user-friendly, portable, and cost-effective in-situ PON/POC biosensors for the detection of biomolecules. Such sensors are well-suited for a variety of applications, including food safety, environmental monitoring, and disease diagnostics. Herein, for the first time, achievements made between 2017 and 2022 in the concept and design of the smartphone-enabled aptasensors for biosensing applications are reviewed. The review covers different fabrication strategies and the discussion of several operating systems, underlying programs, and related software. At the end, the important merits, challenges, and future prospects of smart phone-driven aptasensors are presented. This report intends to assist scientists and engineers in comprehending the fabrication of smartphone-based aptasensors and their underlying sensing principles, as well as stimulating the future developments in the direction of affordable, portable, simple, and readily available sensing devices.
Publisher: MDPI AG
Date: 25-09-2022
DOI: 10.3390/MI13101593
Abstract: The COVID-19 pandemic, caused by a new coronavirus, has affected economic and social standards as governments and healthcare regulatory agencies throughout the world expressed worry and explored harsh preventative measures to counteract the disease’s spread and intensity. Several academics and experts are primarily concerned with halting the continuous spread of the unique virus. Social separation, the closing of borders, the avoidance of big gatherings, contactless transit, and quarantine are important methods. Multiple nations employ autonomous, digital, wireless, and other promising technologies to tackle this coronary pneumonia. This research examines a number of potential technologies, including unmanned aerial vehicles (UAVs), artificial intelligence (AI), blockchain, deep learning (DL), the Internet of Things (IoT), edge computing, and virtual reality (VR), in an effort to mitigate the danger of COVID-19. Due to their ability to transport food and medical supplies to a specific location, UAVs are currently being utilized as an innovative method to combat this illness. This research intends to examine the possibilities of UAVs in the context of the COVID-19 pandemic from several angles. UAVs offer intriguing options for delivering medical supplies, spraying disinfectants, broadcasting communications, conducting surveillance, inspecting, and screening patients for infection. This article examines the use of drones in healthcare as well as the advantages and disadvantages of strict adoption. Finally, challenges, opportunities, and future work are discussed to assist in adopting drone technology to tackle COVID-19-like diseases.
Publisher: Elsevier BV
Date: 12-2022
Publisher: Elsevier BV
Date: 10-2022
Publisher: MDPI AG
Date: 05-09-2023
Publisher: Informa UK Limited
Date: 03-07-2022
Publisher: Bolan Society for Pure and Applied Biology (BSPAB)
Date: 10-12-2016
Publisher: Elsevier BV
Date: 08-2022
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D3TB00641G
Abstract: Fabricating an organic–inorganic nanocomposite hydrogel platform with antibacterial, anti-inflammatory, and osteoinductive properties that mimic bone extracellular matrix composition is decisive for guiding bone development in orthopedic practice.
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0AN00460J
Abstract: We have developed an aptamer discovery method that combines a capillary-based integrated droplet digital PCR system with cytometer sorting of beads in a high-throughput way.
Publisher: Elsevier BV
Date: 05-2022
DOI: 10.1016/J.TALANTA.2022.123302
Abstract: In this paper, we report the development of a new strategy termed integrated digital PCR-fluorescence activated sorting based SELEX (IFS-SELEX) that enables rapid screening of aptamers against fluorescent targets. Initially, this strategy employs an integrated digital PCR system to lify each sequence of a preliminarily enriched library, which is obtained by a traditional SELEX method, on the surface of polystyrene beads. Then, the as-prepared beads are incubated with the fluorescent target and then subjected to fluorescence-activated sorting. Since only those sequences with high binding affinity for the target are collected and sequenced, unnecessary analysis of ineligible sequences is avoided by this method, and the aptamer selection process is thereby greatly streamlined. As a proof-of-concept, we applied this strategy for the screening of aptamers against two fluorescent targets, i.e., ciprofloxacin (CFX) and thioflavin T (ThT), and successfully obtained corresponding sequences with low dissociation constants. The binding affinities of aptamers for ThT were well associated with the sorting regions defined in the fluorescence channel of the flow cytometry process. The experimental results demonstrated that the as-designed IFS-SELEX method can serve as a universal platform for rapid, facile, and efficient aptamer selection against various fluorescent targets.
Publisher: Elsevier BV
Date: 12-2023
Publisher: Informa UK Limited
Date: 27-01-2022
Publisher: MDPI AG
Date: 25-03-2021
DOI: 10.3390/NANO11040840
Abstract: Misuse of antibiotics has recently been considered a global issue because of its harmful effects on human health. Since conventional methods have numerous limitations, it is necessary to develop fast, simple, sensitive, and reproducible methods for the detection of antibiotics. Among numerous recently developed methods, aptasensors are fascinating because of their good specificity, sensitivity and selectivity. These kinds of biosensors combining aptamer with colorimetric applications of gold nanoparticles to recognize small molecules are becoming more popular owing to their advantageous features, for ex le, low cost, ease of use, on-site analysis ability using naked eye and no prerequisite for modern equipment. In this review, we have highlighted the recent advances and working principle of gold nanoparticles based colorimetric aptasensors as promising methods for antibiotics detection in different food and environmental s les (2011–2020). Furthermore, possible advantages and disadvantages have also been summarized for these methods. Finally, the recent challenges, outlook, and promising future perspectives for developing novel aptasensors are also considered.
Publisher: Medwin Publishers
Date: 08-01-2021
Abstract: Background: Nosocomial infections are great threat for hospitalized patients and Pseudomonas aeruginosa has emerged as one of the most potent nosocomial pathogens along with its erse mechanisms to counter the various antimicrobial agents such as aminoglycosides, fluoroquinolones, monobactems, third generation cephalosporins, carbapenams and broadspectrum penicillins. P. aeruginosa is one of the well-known pyogenic bacteria and is 3rd leading cause of pyogenic infections with the variable frequency depending on geographical region and clinical setting. P. aeruginosa is intimately associated with pyogenic nosocomial infections. Objectives: Since multidrug resistant strains of P. aeruginosa have posed serious threats and are frequently implicated in nosocomial infections. Methods: Pus swab were s led under aseptic conditions and cultured on blood and Muller Hinton agar. Gram reaction, pigment production, Oxidase, indole reaction and citrate test were used to confirm isolate. Antibiotic susceptibility was performed b Kirby Bauer technique. Results compiled by us in this cross sectional study, showed 58 cases of P. aeruginosa out of 289 cases. This included 43% males and 57% females. Majority of the patients were of young age, with mean age 38 years. Antibiotic sensitivity revealed resistance to gentamicin was 50%, amikacin was 64%, ciprofloxacin and Aztronem 66%, Cefaparazone 69%, Tzaocin 71% and meropenem and sulzone was 79%. While Colistin and Ceftazidime were the most effective in 85% and 89% of cases respectively. The multidrug resistant strains of P. aeruginosa infections accounted for 32.76% of total P. aeruginosa infections. This study reveals high prevalence of multidrug resistant organisms at the set of our study. Based on this study, we suggest adopting the strategies to minimize the risk of nosocomial infections to slow down the rapidly growing multidrug resistance. These strategies may include, stricter antiseptic measures, fastening the recovery process and reducing the hospital stay and considering other alternates. Besides this, we would like to suggest the precise use of antibiotic susceptibility facility to reduce the nosocomial infection associated complications.
Publisher: Elsevier BV
Date: 07-2022
Publisher: Informa UK Limited
Date: 26-08-2022
Publisher: Journal of Pure and Applied Microbiology
Date: 28-04-2020
No related grants have been discovered for Qurat ul Ain Zahra.