ORCID Profile
0000-0003-4023-9537
Current Organisation
University of Tasmania
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Publisher: Elsevier BV
Date: 02-2016
DOI: 10.1016/J.FOODCHEM.2015.07.090
Abstract: Sugar degradation during grain germination is important for malt quality. In malting industry, gibberellin (GA) is frequently used for improvement of malting quality. In this study, the changes of metabolite profiles and starch-degrading enzymes during grain germination, and as affected by GA and abscisic acid (ABA) were investigated using two wild barley accessions XZ72 and XZ95. Totally fifty-two metabolites with known structures were detected and the change of metabolite during germination was time- and genotype dependent. Sugars and amino acids were the most dramatically changed compounds. Addition of GA enhanced the activities of starch-degrading enzymes, and increased most metabolites, especially sugars and amino acids, whereas ABA had the opposite effect. The effect varied with the barley accessions. The current study is the first attempt in investigating the effect of hormones on metabolite profiles in germinating barley grain, being helpful for identifying the factors affecting barley germination or malt quality.
Publisher: Elsevier BV
Date: 12-2014
DOI: 10.1016/J.FOODCHEM.2014.05.058
Abstract: Our previous study found that the critical protein in SE (silica eluted) proteins is BTI-CMe, and assumed that SE-ve malt for brewing may improve the haze stability in beer. In this study, we investigated the difference in gene sequence and corresponding amino acid sequence of BTI-CMe between SE+ve and SE-ve types. The results showed that there were 7 amino acid differences between Yerong (SE-ve) and Franklin (SE+ve). Two types BTI-CMe were expressed in vitro and purified successfully. By adding the purified BTI-CMe into commercial beer, we found that both original turbidity and alcohol chill haze degree of beer were increased. BTI-CMe of SE-ve haplotype showed a lower level of haze formation in beer than SE+ve haplotype. Response surface methodology (RSM) was conducted to determine the relationship between BTI-CMe and tannic acid, and their effects on haze formation. It was found that (1) higher content of BTI-CMe and/or tannic acid in beer would give rise to higher turbidity (2) there was a significant interaction between BTI-CMe and tannic acid (3) haze activity disparity of BTI-CMe between two types was significantly and positively correlated with the tannic acid concentration.
Publisher: Springer Science and Business Media LLC
Date: 26-04-2017
DOI: 10.1007/S00122-017-2910-8
Abstract: We report the first study on the unique allele from wild barley that can improve waterlogging tolerance in cultivated barley with a substantially higher contribution to aerenchyma formation. Waterlogging is one of the major abiotic stresses that dramatically reduce barley crop yield. Direct selection on waterlogging tolerance in the field is less effective due to its viability to environment. The most effective way of selection is to choose traits that make significant contributions to the overall tolerance and are easy to score. Aerenchyma formation under waterlogging stress is one of the most effective mechanisms to provide adequate oxygen supply and overcome stress-induced hypoxia imposed on plants. In this study, a new allele for aerenchyma formation was identified from a wild barley accession TAM407227 on chromosome 4H. Compared to that identified in cultivated barley, this allele not only produced a greater proportion of aerenchyma but made a greater contribution to the overall waterlogging tolerance. The QTL explained 76.8% of phenotypic variance in aerenchyma formation with a LOD value of 51.4. Markers co-segregating with the trait were identified and can be effectively used in marker assisted selection.
Publisher: Wiley
Date: 29-10-2014
DOI: 10.1002/ETC.2776
Abstract: Phosphate (PO4 (3-) ) has been reported to suppress arsenate (As(v) ) uptake in plants. However, its effects on controlling the availability of As(v) in tobacco genotypes with different arsenic (As) tolerances has not been fully explored. In the present study, the effects of PO4 (3-) on As(v) uptake were investigated in a hydroponic culture using 2 tobacco (Nicotiana tabacum) genotypes (ZY90 and FSMY) that differed in As(v) tolerance. A total of 9 treatment combinations comprising As(v) treatments of 0 µM, 10 µM, and 100 µM and PO4 (3-) treatments of 0 µM, 50 µM, and 500 µM were used. The results showed that ZY90 had greater reductions in leaf photosynthetic parameters, root and shoot dry weight, length, and nutrient content than did FSMY when exposed to As(v) stress. The addition of 500 µM external PO4 (3-) significantly suppressed As(v) (100 µM) uptake in both FSMY and ZY90, with the effect being more pronounced in FSMY. Greater PO4 (3-) uptake in plants significantly reduced the influx of As(v) , causing an increase in photosynthesis and nutrient uptake. Phosphate supply increased superoxide dismutase activity, catalase activity, and malondialdehyde content. The present study showed that PO4 (3-) is an effective competitive inhibitor of As(v) , and it can be effectively used to control As(v) accumulation in tobacco plants.
Publisher: Springer Science and Business Media LLC
Date: 10-07-2017
Publisher: Springer Science and Business Media LLC
Date: 28-02-2015
No related grants have been discovered for Hongliang Hu.