ORCID Profile
0000-0002-8418-5916
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: MDPI AG
Date: 15-09-2022
DOI: 10.3390/MOLECULES27186024
Abstract: Potato (Solanum tuberosum L.) exhibits broad variations in cultivar resistance to tuber and root infections by the soilborne, obligate biotrophic pathogen Spongospora subterranea. Host resistance has been recognised as an important approach in potato disease management, whereas zoospore root attachment has been identified as an effective indicator for the host resistance to Spongospora root infection. However, the mechanism of host resistance to zoospore root attachment is currently not well understood. To identify the potential basis for host resistance to S. subterranea at the molecular level, twelve potato cultivars differing in host resistance to zoospore root attachment were used for comparative proteomic analysis. In total, 3723 proteins were quantified from root s les across the twelve cultivars using a data-independent acquisition mass spectrometry approach. Statistical analysis identified 454 proteins that were significantly more abundant in the resistant cultivars 626 proteins were more abundant in the susceptible cultivars. In resistant cultivars, functional annotation of the proteomic data indicated that Gene Ontology terms related to the oxidative stress and metabolic processes were significantly over-represented. KEGG pathway analysis identified that the phenylpropanoid biosynthesis pathway was associated with the resistant cultivars, suggesting the potential role of lignin biosynthesis in the host resistance to S. subterranea. Several enzymes involved in pectin biosynthesis and remodelling, such as pectinesterase and pectin acetylesterase, were more abundant in the resistant cultivars. Further investigation of the potential role of root cell wall pectin revealed that the pectinase treatment of roots resulted in a significant reduction in zoospore root attachment in both resistant and susceptible cultivars. This study provides a comprehensive proteome-level overview of resistance to S. subterranea zoospore root attachment across twelve potato cultivars and has identified a potential role for cell wall pectin in regulating zoospore root attachment.
Publisher: Shanghai Institute of Organic Chemistry
Date: 2012
DOI: 10.6023/CJOC1112192
Publisher: Wiley
Date: 25-10-2023
DOI: 10.1111/PPA.13659
Abstract: Spongospora subterranea f. sp. subterranea is a major pathogen of potatoes leading to losses in tuber quality and yield. Disease can be expressed as root infection, root galling and tuber lesions, the latter known as powdery scab. Attachment of zoospores to potato root hairs is the first step before infection of roots and disease development. Root hair infection results in root dysfunction leading to impaired plant productivity and yield. Varieties vary in their susceptibility to root and tuber disease however, varietal screening is both time and resource intensive. Furthermore, traditional screens assess root galling or tuber disease and not root infection. In this study, we determined optimal conditions for zoospore release and attachment of zoospores to plant roots and used this information to develop an in vitro bioassay to assess resistance to zoospore root attachment among 153 potato lines and cultivars. Optimal zoospore release occurred at 20°C in Hoagland's solution in a rapid and synchronized manner over the first 2 days, followed by a steep decline. The extent of zoospore root attachment varied with cultivar (Iwa Agria Russet Burbank Gladiator), region of the root maturation zone (lower middle upper) and temperature (greatest zoospore root attachment occurring at 15°C). Further comparisons suggested efficiency of zoospore root attachment was also generally associated with known variety resistance to powdery scab, zoosporangial infection and root galling, with a few notable exceptions. The bioassay proved to be a rapid and robust method for screening cultivar resistance to zoospore root attachment.
Publisher: MDPI AG
Date: 09-02-2023
DOI: 10.3390/PROTEOMES11010007
Abstract: For potato crops, host resistance is currently the most effective and sustainable tool to manage diseases caused by the plasmodiophorid Spongospora subterranea. Arguably, zoospore root attachment is the most critical phase of infection however, the underlying mechanisms remain unknown. This study investigated the potential role of root-surface cell-wall polysaccharides and proteins in cultivars resistant/susceptible to zoospore attachment. We first compared the effects of enzymatic removal of root cell-wall proteins, N-linked glycans and polysaccharides on S. subterranea attachment. Subsequent analysis of peptides released by trypsin shaving (TS) of root segments identified 262 proteins that were differentially abundant between cultivars. These were enriched in root-surface-derived peptides but also included intracellular proteins, e.g., proteins associated with glutathione metabolism and lignin biosynthesis, which were more abundant in the resistant cultivar. Comparison with whole-root proteomic analysis of the same cultivars identified 226 proteins specific to the TS dataset, of which 188 were significantly different. Among these, the pathogen-defence-related cell-wall protein stem 28 kDa glycoprotein and two major latex proteins were significantly less abundant in the resistant cultivar. A further major latex protein was reduced in the resistant cultivar in both the TS and whole-root datasets. In contrast, three glutathione S-transferase proteins were more abundant in the resistant cultivar (TS-specific), while the protein glucan endo-1,3-beta-glucosidase was increased in both datasets. These results imply a particular role for major latex proteins and glucan endo-1,3-beta-glucosidase in regulating zoospore binding to potato roots and susceptibility to S. subterranea.
Location: Australia
No related grants have been discovered for XIAN YU.