ORCID Profile
0000-0002-0525-8954
Current Organisation
Murdoch University
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Publisher: Wiley
Date: 04-2021
DOI: 10.1111/AVJ.13061
Publisher: University of Toronto Press Inc. (UTPress)
Date: 09-2017
Abstract: Four years after the institution of a new curriculum at the University of Pretoria's Faculty of Veterinary Science, a second major curricular renewal was initiated as a result of several pressing drivers, many of which were unique to the national and institutional context. During the project, South African higher education was disrupted by student protests prompted by financial pressures on students, the overt colonial structure of higher education, and the need to accelerate transformation of the sector. A structured curricular renewal process was followed, including the laying down of the principles, the structuring the macro-curriculum as a story, the design of a meso- and micro-curriculum, and the mapping of the curriculum to Day One outcomes. The resulting program is a 6-year bachelor's degree with a blend of discipline and species modules, with the first cohort graduating in 2016. There is a strong focus on skills embodied in a 62-week experiential component, managed using a unique custom-designed online platform for booking placements, documenting exposure, assessing competency, and providing feedback to students. The experiential training includes a large elective component. Several causes of loss of impetus during the process are discussed and proposals are made for avoiding these. The value of accreditation as a driver and a source of inputs is evident. The process has succeeded in producing a significantly reshaped curriculum that has been well received by external stakeholders.
Publisher: American Veterinary Medical Association (AVMA)
Date: 15-10-2014
Abstract: Objective —To determine the perceived importance of specific competencies in professional veterinary practice and education among veterinarians in several countries. Design —Survey-based prospective study. S le —1,137 veterinarians in 10 countries. Procedures —Veterinarians were invited via email to participate in the study. A framework of 18 competencies grouped into 7 domains (veterinary expertise, communication, collaboration, entrepreneurship, health and welfare, scholarship, and personal development) was used. Respondents rated the importance of each competency for veterinary professional practice and for veterinary education by use of a 9-point Likert scale in an online questionnaire. Quantitative statistical analyses were performed to assess the data. Results —All described competencies were perceived as having importance (with overall mean ratings [all countries] ≥ 6.45/9) for professional practice and education. Competencies related to veterinary expertise had the highest ratings (overall mean, 8.33/9 for both professional practice and education). For the veterinary expertise, entrepreneurship, and scholarship domains, substantial differences (determined on the basis of statistical significance and effect size) were found in importance ratings among veterinarians in different countries. Conclusions and Clinical Relevance —Results indicated a general consensus regarding the importance of specific types of competencies in veterinary professional practice and education. Further research into the definition of competencies essential for veterinary professionals is needed to help inform an international dialogue on the subject.
Publisher: Wiley
Date: 05-04-2022
DOI: 10.1111/AVJ.13162
Abstract: The objective of this study was to determine the prevalence of gastrointestinal nematodes among post‐weaned calves aged between 4 and 12 months old within a pasture‐based system of south west Australia and quantify the level of anthelmintic resistance. Pre‐treatment FECs were monitored on 14 dairy farms. Anthelmintic resistance was assessed on 11 of the farms. Control FECs were compared with anthelmintic FECs at 14 days post‐treatment with doramectin (injectable), levamisole (oral), fenbendazole (oral) and a levamisole/abamectin combination (pour‐on). Results demonstrate a strong level of anthelmintic resistance, with at least one class of anthelmintic failing to achieve a 95% reduction in FEC in one or more gastrointestinal nematode species. Doramectin was fully effective against Ostertagia , but C. oncophora displayed resistance in 91% of the farms. Conversely, levamisole was fully effective against C. oncophora , but Ostertagia displayed resistance in 80% of the farms. Fenbendazole resistance was present in both C. onocphora and Ostertagia in 64% and 70% of the farms, respectively. Trichostrongylus showed low resistance, occurring in doramectin (14%) and levamisole/abamectin combination (14%). This study confirms that anthelmintic resistance is common. Regular FEC reduction testing is recommended to monitor and guide decision‐making for appropriate anthelmintic usage.
Publisher: Elsevier BV
Date: 03-2007
DOI: 10.1016/J.VACCINE.2006.12.010
Abstract: Twelve serologically negative bulls were used, six were vaccinated with a modified live LSD vaccine and six unvaccinated. All were then experimentally infected with a virulent field strain of LSDV. No clinical abnormality was detected following vaccination, and mild clinical signs were seen in four vaccinated bulls following challenge. Virus was not found in semen of vaccinated bulls. Two of the unvaccinated bulls developed severe LSD and four showed mild symptoms, all excreted the virus in the semen following challenge. This study confirmed the ability of LSD vaccination to prevent the excretion of LSDV in semen of vaccinated bulls.
Publisher: Wiley
Date: 03-03-2022
DOI: 10.1111/AVJ.13152
Abstract: This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to purulent vaginal discharge 60–100 days postpartum. Vaginal discharge s les were obtained, cultured, identified and tested for antimicrobial susceptibility. A total of 118 bacterial isolates were grown from 46 animals, representing 36 species. The bacteria isolated from both aerobic and anaerobic cultures included Bacillus (60.2%), Streptococcus (12.7%), Trueperella (10.1%), Escherichia (6.7%) and Staphylococcus (5.9%). The remaining genera % were Histophilus , Aeroccocus , Enterococcus and Moraxella . Resistance was variable between isolates, but the highest resistance levels were observed in Streptococcal and Bacillus isolates to enrofloxacin, clindamycin and erythromycin, respectively. All Streptococcal isolates exhibited 100% resistance to enrofloxacin, and the greatest resistance levels were found in Streptococcus luteinises to trimethoprim‐sulfamethoxazole 83%, clindamycin 66% and 33% quinupristin‐dalfopristin. There was 84.5% resistance to clindamycin and 35.2% to erythromycin in the Bacillus isolates, with the highest resistance found in Bacillus licheniformis and Bacillus subtilis . Escherichia coli exhibited 12.5% resistance to gentamycin, ceftiofur, whereas amoxicillin‐clavulanic acid exhibited 37.5%. Within the Staphylococcal isolates, 28.5%, 28.5%, 42.8% and 14.2% resistance to ceftiofur, erythromycin, cefoxitin, penicillin and tetracycline were observed, respectively. The presence of resistance to important antimicrobials for human use, such as cephalosporins, macrolides and fluoroquinolones, highlights the need for judicious use of antimicrobials in dairy cattle.
Publisher: CSIRO Publishing
Date: 2008
DOI: 10.1071/WR07136
Abstract: The aim of this study was to evaluate the effect of active immunisation against GnRH on ovarian activity and serum progesterone concentrations in a large group of mares (10 control and 55 experimental) under field conditions as a model for wildlife species such as zebra and African elephants. Within the experimental group, mares were sub ided into three age categories: Category 1 (4 years and younger, n = 26), Category 2 (4–10 years old, n = 18), and Category 3 (≥11 years old, n = 11). Experimental mares were vaccinated intramuscularly with 2 mL (400 μg) of the GnRH vaccine Improvac (Pfizer Animal Health, Sandton, South Africa). Control mares received the same amount of saline solution. The vaccinations were repeated 35 days later. The ovaries and reproductive tracts of each mare were examined by means of rectal palpation and ultrasonography on Days 0, 35 and 70. Blood was collected weekly for determination of serum progesterone concentration until Day 175. On Day 35 after primary vaccination all of the control mares and 14.5% of the experimental mares showed evidence of ovarian activity on the basis of clinical examination and serum progesterone concentration. On Day 70, all control mares and none of the experimental mares showed evidence of cyclic activity. No age-related effect within treatment groups was found. The serum progesterone concentration indicated that all experimental mares remained in anoestrus until Day 175. Five of the control mares fell pregnant between Days 35 and 70. The five non-pregnant control mares continued to cycle until the end of the observation period. Having achieved such promising results in this trial we now plan to test the GnRH vaccine in Burchell’s zebra mares and African elephant cows.
Publisher: University of Toronto Press Inc. (UTPress)
Date: 05-2018
Abstract: To optimize bovine pregnancy diagnosis (PD) training, factors influencing student performance were investigated. The objective was to determine whether training method, gender, background (farm, urban, or mixed), previous experience in bovine PD, and current career interest influenced the accuracy of bovine PD by trans-rectal palpation (TRP). Fourth-year (of a 6-year program) veterinary students (n=138) received one PD training session in groups using either simulator training on Breed'n Betsy (BB) or training on live cows (C). Students completed a questionnaire on gender, background, and career interest. Students' PD accuracy (pregnancy status and stage) was determined after training when each student palpated six cows with known pregnancy status. Students' accuracy in determining pregnancy status was measured as sensitivity and specificity (the ability to correctly identify the presence and absence of pregnancy respectively). Factors that influenced overall accuracy with a higher student sensitivity of bovine PD by TRP were training method, farming background, an interest in a mixed animal career, and stage of gestation. Gender of students and previous experience in bovine PD did not have an influence. Training on BB simulators was associated with lower student sensitivity for pregnancy detection in cows months pregnant. Student sensitivity for pregnancy detection in cows months pregnant was similar for training on BB simulators and live cows. No evaluated factors were significantly associated with specificity of PD. Teaching efforts focusing on specificity of PD and repeated simulator-based training in conjunction with live cow exposure are recommended.
Publisher: Hindawi Limited
Date: 02-09-2020
DOI: 10.1111/TBED.13670
Publisher: Medpharm Publications
Date: 09-2022
DOI: 10.36303/JSAVA.02
Publisher: Wiley
Date: 04-2010
DOI: 10.1111/J.1439-0531.2008.01274.X
Abstract: The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell-rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen s le collected on day 28 p.i. were slaughtered and tissue s les from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell-rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem s les of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate.
Publisher: Hindawi Limited
Date: 05-01-2014
DOI: 10.1111/TBED.12045
Abstract: It is known that lumpy skin disease virus (LSDV) can be shed in bull semen following infection and also that artificial insemination (AI) poses a biosecurity risk. However, it is not known whether the use of LSDV infected semen in AI poses a biosecurity risk. The aim of this study was to investigate whether LSDV, transmitted through semen, can infect cows and their embryos. Two controlled trials were performed simultaneously. Eleven young beef heifers, naïve to LSDV, were synchronized using an OvSynch protocol and inseminated on Day 0 with fresh semen spiked with a field strain of LSDV on day 0. Six of the heifers were superovulated on Day 1 using pregnant mare serum gonadotropin, and embryos were flushed from these heifers on Day 6. Blood and serum s les were collected from Day 4 until Day 27 to determine the presence of LSDV by PCR and virus isolation, and the presence of antibodies against LSDV by SNT. The first clinical signs of LSD were noticed on Day 10, followed by severe generalized LSD in three heifers and mild LSD in two more heifers. Two heifers were humanely euthanized due to severe unresponsive stranguria. LSDV was detected by PCR, virus isolation or electron microscopy in blood, embryos and organs of experimentally infected animals and eight heifers had seroconverted by Day 27. Two control animals were not affected. This is the first report of experimental seminal transmission of LSDV in cattle.
Publisher: AOSIS
Date: 27-02-2015
Abstract: Rift Valley fever (RVF) is an arthropod-borne viral disease of importance in livestock and humans. Epidemics occur periodically in domestic ruminants. People in contact with infected livestock may develop disease that varies from mild flu-like symptoms to fatal viraemia. Livestock vaccination may assist in disease control. Rift Valley fever virus (RVFV) Clone 13 is a relatively new vaccine against RVF, derived from an avirulent natural mutant strain of RVFV, and has been shown to confer protective immunity against experimental infection with RVFV. The hypothesis tested in the current trial was that rams vaccinated with RVFV Clone 13 vaccine would not experience a reduction in semen quality (measured by evaluating the percentage progressively motile and percentage morphologically normal spermatozoa in successive ejaculates) relative to unvaccinated control animals. Ram lambs were screened for antibodies to RVFV using a serum neutralisation test. Animals without detectable antibodies (n = 23) were randomly allocated to either a test group (n = 12) or a control group (n = 11). Animals in the test group were vaccinated with RVFV Clone 13 vaccine. Daily rectal temperature measurements and weekly semen and blood s les were taken from all animals. Seven animals were eliminated from the statistical analysis because of potential confounding factors. Logistic regression analysis was performed on data gathered from the remaining animals to determine whether an association existed between animal group, rectal temperature and semen quality parameters. No correlation existed between the treatment group and values obtained for the semen quality parameters measured. There was no statistically significant post-vaccination decline in the percentage of live morphologically normal spermatozoa, or the percentage of progressively motile spermatozoa, either when assessed amongst all animals or when assessed within in idual groups. A repeat study with a larger s le size and a more comprehensive pre-screening process may be indicated to avoid the inclusion of unsuitable animals.
Publisher: Hindawi Limited
Date: 07-04-2019
DOI: 10.1111/TBED.13179
Abstract: Lumpy skin disease (LSD) is an important transboundary animal disease of cattle with significant economic impact because of the implications for international trade in live animals and animal products. LSD is caused by a Capripoxvirus, LSD virus (LSDV), and results in extensive hide and udder damage, fever and pneumonia. LSDV can be shed in semen of infected bulls for prolonged periods and transmitted venereally to cows at high doses. This study examined the effects of LSDV in frozen-thawed semen on in vitro embryo production parameters, including viral status of media and resulting embryos. Bovine oocytes were harvested from abattoir-collected ovaries and split into three experimental groups. After maturation, the oocytes were fertilized in vitro with frozen-thawed semen spiked with a high (HD) or a lower (LD) dose of LSDV, or with LSDV-free semen (control). Following day 7 and day 8 blastocyst evaluation, PCR and virus isolation were performed on all embryonic structures. After completing sufficient replicates to reach 1,000 inseminated oocytes, further in vitro fertilization (IVF) runs were performed to provide material for electron microscopy (EM) and embryo washing procedures. Overall, in vitro embryo yield was significantly reduced by the presence of LSDV in frozen-thawed semen, irrespective of viral dose. When semen with a lower viral dose was used, significantly lower oocyte cleavage rates were observed. LSDV could be detected in fertilization media and all embryo structures, when higher doses of LSDV were present in the frozen-thawed semen used for IVF. Electron microscopy demonstrated LSDV virions inside blastocysts. Following the International Embryo Transfer Society washing procedure resulted in embryos free of viral DNA however, this may be attributable to a s ling dilution effect and should be interpreted with caution. Further research is required to better quantify the risk of LSDV transmission via assisted reproductive procedures.
Publisher: Medpharm Publications
Date: 13-08-2015
Abstract: Salmonellosis is a common healthcare-associated infection in large-animal hospitals, and surveillance for Salmonella is an integral part of comprehensive infection control programmes in populations at risk. The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental s les obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. Environmental s les were collected using household cleaning cloths that were incubated overnight in buffered peptone water (BPW). Aliquots of BPW were then processed using two different selective enrichment and culture techniques. In the first technique (TBG-RV-XLT4) s les were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. The second technique (SEL-XLD) involved incubation at 37 °C in selenite broth before plating on XLD agar. Salmonella was recovered from 49.7% (73/147) of s les using the TBG-RV-XLT4 technique, but only 10.2% (15/147) of s les using the SEL-XLD method. Fourteen s les (9.5%) were culture-positive using both methods, and 73 (49.7%) were culture-negative using both techniques. There were discordant results for 60 s les, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one s le that was only culturepositive using the SEL-XLD method. Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. Environmental contamination with Salmonella may be underestimated by certain culture techniques, which may impair efforts to control spread in veterinary hospitals.
Publisher: Springer Science and Business Media LLC
Date: 2012
Publisher: Elsevier BV
Date: 12-2021
DOI: 10.1016/J.RVSC.2021.10.005
Abstract: Mastitis is an economically important production disease in the dairy industry worldwide. There is limited information on the aetiology of clinical mastitis (CM) and subclinical mastitis (SCM) in Australia's Mediterranean pasture-based production system. A prospective study was conducted in the south-west region of Western Australia to characterise the bacterial pathogens associated with CM and SCM cases and their associated antimicrobial susceptibility profiles. A total of 102 CM and 132 SCM milk s les were collected in twelve dairy farms between April 2020 and September 2020 recovering a total of 310 bacterial isolates. The isolates were evaluated for their antimicrobial susceptibility to twelve antibiotics using the agar disk diffusion (ADD) method. The most common pathogens associated with CM was Bacillus spp. (35.29%), followed by coagulase-negative staphylococci (CNS) (22.55%), Pseudomonas spp. (19.61%), Staphylococcus aureus (10.78%), Escherichia coli (5.88%) and Streptococcus uberis (2.94%). The most common pathogens associated with SCM was CNS (44.70%), followed by Bacillus spp. (30.30%), S. aureus (20.45%), Strep. uberis (15.91%), coliforms (Citrobacter spp., Cronobacter spp., Enterobacter spp., Klebsiella spp., Kosakonia spp., Morganella spp., Serratia spp.) (9.86%), environmental Streptococci (6.06%) and E. coli (6.06%). Beta-lactams resistance was the most common resistance observed in the Staphylococcal isolates and a high proportion of Streptococcal isolates exhibited resistance to enrofloxacin. Overall, the proportion of bacterial pathogens isolated in this study was comparable to the figures reported in other studies in Australia. Future research should focus on risk factors and the determination of resistant genetic components among the common isolates.
Publisher: Medpharm Publications
Date: 05-12-2018
Abstract: Salmonellosis is a disease of major zoonotic importance and canine parvovirus is a potentially fatal cause of canine enteritis with a world-wide distribution. Persistent isolation of Salmonella during routine environmental s ling surveys of a hospital ward, reserved for the treatment of dogs with canine parvovirus infection, prompted investigation into a possible source. We hypothesised that dogs affected by canine parvovirus would have a higher prevalence of faecal salmonellae compared to an apparently healthy cohort. Seventy-four client-owned dogs naturally infected with canine parvovirus and 42 apparently healthy client-owned dogs were included in the study. This prospective, longitudinal, observational study was conducted over an 18-month period. Fresh faecal s les were collected from dogs aged 6 weeks to 9 months diagnosed with canine parvovirus infection and admitted for treatment, and from apparently healthy dogs presented for vaccination or routine hospital procedures. Faeces were submitted for the isolation, antimicrobial susceptibility testing and serotyping of salmonellae. The prevalence of faecal Salmonella shedding was 22% and 31% for the affected and apparently healthy dogs, respectively, which was not statistically different. No significant associations between Salmonella status and possible risk factors or continuous variables such as age, body weight and duration of hospitalisation were identified. All the Salmonella isolates (n = 32) were resistant to penicillin G, lincomycin and tylosin. Salmonellae from nine different serotypes were identified. The prevalence of Salmonella shedding in both groups was higher than that commonly reported, yet similar to those in previous reports on young dogs, shelter dogs or dogs fed a raw meat diet.
Publisher: AOSIS
Date: 17-07-2018
Abstract: The prevalence of nasal carrier status of methicillin-resistant Staphylococcus aureus (MRSA) in pigs has been described elsewhere, but is unknown in South Africa. To address concerns that exist regarding the zoonotic risk that carriers pose to workers, the herd-level prevalence of MRSA was determined among 25 large ( 500 sows) commercial pig herds in South Africa, representing 45% of the large commercial herds in the country. From each herd, the nasal contents of 18 finisher pigs were s led at the abattoir, pooled into three and selectively cultured to determine the presence of MRSA. A herd was classified as MRSA-positive if one or more of the three pooled s les cultured positive. Three of the 25 herds tested positive for MRSA, equating to a 12% herd prevalence (95% CI: 7% – 23%) among South African commercial piggeries. The prevalence of nasal MRSA carriers among large commercial pig herds in South Africa was low compared to what has been reported elsewhere and suggests a relatively low zoonotic MRSA risk to workers in South African commercial piggeries and abattoirs.
Publisher: Elsevier BV
Date: 08-2018
DOI: 10.1016/J.ANIREPROSCI.2018.04.080
Abstract: Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR. The ejaculate was split into a control s le (C), a s le spiked with a 4 log TCID
Publisher: MDPI AG
Date: 31-08-2023
DOI: 10.3390/V15091861
Publisher: Society for Theriogenology
Date: 30-05-2023
DOI: 10.58292/CT.V15.9590
Abstract: This article describes the implementation of an educational intervention, ‘bovine pregnancy diagnosis challenge’ over an 8-year period, and how evidence-based changes were applied to ensure the best learning outcome for bovine pregnancy diagnosis (PD) via transrectal palpation. With the worldwide shift from mainly knowledge-based to competency-based veterinary courses, many new and innovative teaching interventions have been introduced into veterinary education. These teaching interventions include but are not limited to simulators, models, virtual realities, applications, and computer-assisted learning tools of which, many have been implemented into various veterinary training programs. While these advances are noteworthy, the question still remains as to whether or not the implementation of these teaching interventions is optimal. Do we know the ‘when,’ ‘who’, and ‘how’ of a specific intervention that will optimize the educational outcome? The described evolution of the bovine PD challenge shows how these questions were investigated for transrectal palpation skills, and how changes were implemented to optimize student training. This is an ex le of an approach that could be applied widely for validation and skills training investigations within veterinary education to optimize learning outcomes.
No related grants have been discovered for Cornelius Annandale.