ORCID Profile
0000-0002-1922-0009
Current Organisations
Murdoch University
,
University of Glasgow
,
Perron Institute for Neurological and Translational Sciences
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Publications
Knowledge reuse in edge computing environments
Publisher: Elsevier BV
Date: 10-2022
Goat endometrial heat shock protein-90 (Hsp-90): development of an expedient method for its purification and observations on its intracellular movement.
Publisher: Elsevier BV
Date: 05-2009
DOI: 10.1016/J.PEP.2009.11.006
Abstract: An expedient method has been developed by which goat uterine Hsp-90 could be isolated and purified to homogeneity in less than 1day. The yield is roughly 1mg from 60g tissue. This method takes into advantage three of our earlier observation that (a) Hsp-90 gets linked to the non-activated estrogen receptor (naER) in the presence of 10mM sodium molybdate (b) naER, but not Hsp-90 binds to phosphocellulose and (c) exposure to estradiol facilitates dissociation of Hsp-90 from naER through estradiol binding to naER and the possible change in naER conformation. Intracellular movement of Hsp-90 and naER was monitored in goat endometrial cells in culture following exposure of the cells to estradiol. Confocal microscopic analysis revealed a clear presence of both proteins within the nucleus within 3h after exposure to estradiol. Whether Hsp-90 has its own nuclear-transport machinery is debatable. Being an actin-binding protein, there is a distinct possibility that the nuclear entry of Hsp-90 is actin dependent. The functional significance of the nuclear entry of Hsp-90, along with naER, remains to be determined it may, however, be speculated that the Hsp-90 might be directly involved in the naER to nER II transformation by functioning as a molecular chaperone and helping the protein in re-orienting its structural organization.
Implications of increased S100β and Tau5 proteins in dystrophic nerves of two mdx mouse models for Duchenne muscular dystrophy.
Publisher: Elsevier BV
Date: 06-2020
Age-related loss of VGLUT1 excitatory, but not VGAT inhibitory, immunoreactive terminals on motor neurons in spinal cords of old sarcopenic male mice.
Publisher: Springer Science and Business Media LLC
Date: 06-08-2018
DOI: 10.1007/S10522-018-9765-5
Abstract: Age-related changes in ventral lumbar spinal cord (L3-L5) were compared in young [3 month, (M)] and old (27 M) C57BL/6J male mice. The aged mice had previously been shown to exhibit sarcopenia and changes to peripheral nerve morphology. The putative connectivity of β-III tubulin positive α-motor neurons was compared in immunostained transverse sections using excitatory and inhibitory terminal markers vesicular glutamate transporter-1 (VGLUT1) and vesicular GABA transporter (VGAT). Glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) immunostaining was used to monitor changes in astrocyte and microglial phenotype respectively. For a given motor neuron, the neuronal perimeter was outlined and terminals immunoreactive for VGLUT1 or VGAT in close apposition to the soma were identified. By 27 M, the percentage coverage and total number of VGLUT1 immunoreactive terminals immediately adjacent to the soma of α-motor neurons was significantly decreased compared with young mice. However, percentage coverage of immunoreactive VGAT inhibitory terminals did not change significantly with age. The gray matter of 27 M spinal cords showed increased astrocytic and microglial activity. The loss of VGLUT1 terminals on α-motor neurons, terminals known to be derived from proprioceptive muscle afferents, may further impair sensorimotor control of hind limb skeletal muscle function in old mice.
Regeneration of adult rat sensory and motor neuron axons through chimeric peroneal nerve grafts containing donor Schwann cells engineered to express different neurotrophic factors
Publisher: Elsevier BV
Date: 08-2020
Resistance wheel exercise from mid-life has minimal effect on sciatic nerves from old mice in which sarcopenia was prevented
Publisher: Springer Science and Business Media LLC
Date: 08-06-2017
DOI: 10.1007/S10522-017-9714-8
Abstract: The ability of resistance exercise, initiated from mid-life, to prevent age-related changes in old sciatic nerves, was investigated in male and female C57BL/6J mice. Aging is associated with cellular changes in old sciatic nerves and also loss of skeletal muscle mass and function (sarcopenia). Mature adult mice aged 15 months (M) were subjected to increasing voluntary resistance wheel exercise (RWE) over a period of 8 M until 23 M of age. This prevented sarcopenia in the old 23 M aged male and female mice. Nerves of control sedentary (SED) males at 3, 15 and 23 M of age, showed a decrease in the myelinated axon numbers at 15 and 23 M, a decreased g-ratio and a significantly increased proportion of myelinated nerves containing electron-dense aggregates at 23 M. Myelinated axon and nerve diameter, and axonal area, were increased at 15 M compared with 3 and 23 M. Exercise increased myelinated nerve profiles containing aggregates at 23 M. S100 protein, detected with immunoblotting was increased in sciatic nerves of 23 M old SED females, but not males, compared with 15 M, with no effect of exercise. Other neuronal proteins showed no significant alterations with age, gender or exercise. Overall the RWE had no cellular impact on the aging nerves, apart from an increased number of old nerves containing aggregates. Thus the relationship between cellular changes in aging nerves, and their sustained capacity for stimulation of old skeletal muscles to help maintain healthy muscle mass in response to exercise remains unclear.
Dystrophic Dmdmdx rats show early neuronal changes (increased S100β and Tau5) at 8 months, supporting severe dystropathology in this rodent model of Duchenne muscular dystrophy.
Publisher: Elsevier BV
Date: 10-2020
Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate.
Publisher: Hindawi Limited
Date: 2015
DOI: 10.1155/2015/109353
Abstract: Autologous fat grafting for soft tissue reconstruction is challenged by unpredictable long-term graft survival. Fat derived stromal vascular fraction (SVF) is gaining popularity in tissue reconstruction as SVF-enriched fat grafts demonstrate improved engraftment. SVF also has potential in regenerative medicine for remodeling of ischemic tissues by promoting angiogenesis. Since SVF cells do not require culture expansion, attempts are being made to develop automated devices to isolate SVF at the point of care. We report development of a closed, automated system to process up to 500 mL lipoaspirate using cell size-dependent filtration technology. The yield of SVF obtained by automated tissue digestion and filtration (1.17 ± 0.5 × 10 5 cells/gram) was equivalent to that obtained by manual isolation (1.15 ± 0.3 × 10 5 p = 0.8), and the viability of the cells isolated by both methods was greater than 90%. Cell composition included CD34+CD31− adipose stromal cells, CD34+CD31+ endothelial progenitor cells, and CD34−CD31+ endothelial cells, and their relative percentages were equivalent to SVF isolated by the manual method. CFU-F capacity and expression of angiogenic factors were also comparable with the manual method, establishing proof-of-concept for fully automated SVF isolation, suitable for use in reconstructive surgeries and regenerative medicine applications.
Cytoskeletal elements and intracellular transport.
Publisher: Wiley
Date: 30-04-2007
DOI: 10.1002/JCB.21347
Abstract: Recent advances in the understanding of the functions of various components of the cytoskeleton indicate that, besides serving a structural role, the cytoskeletal elements may regulate the transport of several proteins in the cell. Studies reveal that there are co-operative interactions between the actin and microtubule cytoskeletons including functional overlap in the transport influenced by different motor families. Multiple motors are probably involved in the control of the dynamics of many proteins and intriguing hints about how these motors are co-ordinated are appearing. It has been shown that some of the intermediate elements also participate in selected intracellular transport mechanisms. In view of the author's preoccupation with the steroid receptor systems, special attention has been given to the role of the cytoskeletal elements, particularly actin, in the intracellular transport of steroid receptors and receptor-related proteins.
A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice
Publisher: Oxford University Press (OUP)
Date: 30-03-2016
DOI: 10.1093/JNEN/NLW019
Abstract: To elucidate the neural basis for age-related sarcopenia, we quantified morphologic and molecular changes within sciatic nerves of aging male and female C57BL/6J mice aged between 3 and 27 months using immunoblotting, immunohistochemistry, and electron microscopy. Protein analyses by immunoblotting of nerves of male mice aged 4, 15, 18, 22, and 24 months showed increased levels of heavy chain SMI-32-positive neurofilaments, vimentin, tau5, choline acetyltransferase (ChAT), and p62 by 18-22 months. Similar protein increases were seen in 26-month-old compared with 3-month-old female mice. Immunostaining of longitudinal sections of old (27-month-old) male sciatic nerves revealed intense staining for tau5 and p62 that was increased compared with that at 3 months, but there were decreased numbers of axon profiles stained for ChAT or isolectin B4 (motor and sensory axons, respectively). Ultrastructural analysis revealed electron-dense aggregates within axons in peripheral nerves of old male mice the proportion of axons that contained aggregates more than doubled between 15 and 27 months. Overall, the observed age-related accumulation of many proteins from about 18 months of age onward suggests impaired mechanisms for axonal transport and protein turnover. These peripheral nerve changes may contribute to the morphological and functional muscle deficits associated with sarcopenia.
Related Organisations
University Of Glasgow
Location: United Kingdom of Great Britain and Northern Ireland
Perron Institute For Neurological And Translational Sciences
Location: Australia
Related Funding Activities
No related grants have been discovered for Vidya Krishnan.